CLB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CLB1
Entrez Gene ID	853002
Full Name	B-type cyclin CLB1
Synonyms	SCB1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181237.1	NP_011622.1	B-type cyclin CLB1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that meiosis-specific phosphorylation of B-cyclin Clb1 requires its import to the nucleus.
Title: CDK-dependent nuclear localization of B-cyclin Clb1 promotes FEAR activation during meiosis I in budding yeast.

inactivation of the negative regulatory system by deletion of the HIR1 gene relieved the dependence of HTA1-HTB1 transcription on the mitotic Clb1/Cb2 cyclins
Title: The mitotic Clb cyclins are required to alleviate HIR-mediated repression of the yeast histone genes at the G1/S transition.

Deletion of CLB1 or CLB3 allows cdc14-arrested cells to complete cytokinesis
Title: Functions of the mitotic B-type cyclins CLB1, CLB2, and CLB3 at mitotic exit antagonized by the CDC14 phosphatase.

results show that the FEAR network acts in part by stimulating phosphorylation of Net1 by Clb-Cdk, which directly weakens the interaction of Cdc14 with Net1, allowing for Cdc14 activation and mitotic exit
Title: Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus.

The following CLB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02333	NM_001181237.1 Latest version!	Saccharomyces cerevisiae S288C B-type cyclin CLB1 (CLB1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLB1 ( NM_001181237.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011622.1 Saccharomyces cerevisiae S288C B-type cyclin CLB1 (CLB1), partial mRNA.

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CloneID	OSi02333
Clone ID Related Accession (Same CDS sequence)	NM_001181237.1
Accession Version	NM_001181237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	B-type cyclin CLB1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCACGAT CCCTTTTGGT AGAGAATAGT AGAACCATTA ATAGTAATGA AGAGAAGGGG 
GTAAACGAAA GTCAATATAT CTTACAAAAG AGAAACGTCC CAAGGACCAT TCTCGGTAAT 
GTCACAAATA ATGCAAATAT CCTGCAAGAG ATTTCTATGA ACAGAAAAAT TGGGATGAAG 
AACTTCTCAA AATTGAACAA CTTCTTTCCT TTAAAGGATG ATGTTTCGAG AGCCGATGAC 
TTCACCTCCT CTTTTAATGA CAGTAGACAG GGGGTGAAAC AAGAAGTACT AAACAATAAG 
GAAAATATTC CTGAATATGG ATATTCCGAG CAAGAAAAGC AGCAGTGCTC AAATGATGAC 
TCTTTTCATA CGAATTCGAC TGCCTTGAGC TGTAATCGGT TGATATACTC TGAGAACAAG 
TCTATTTCCA CTCAAATGGA ATGGCAGAAA AAAATAATGA GAGAAGATTC CAAAAAAAAA 
AGGCCAATAT CAACTCTTGT AGAGCAGGAT GACCAGAAAA AGTTCAAACT CCATGAATTG 
ACTACAGAAG AAGAGGTTCT GGAGGAGTAC GAATGGGATG ACCTAGATGA AGAAGATTGT 
GATGATCCCT TAATGGTGAG CGAAGAGGTT AATGATATCT TCGATTATTT GCATCACTTG 
GAAATAATCA CATTGCCAAA TAAGGCGAAT CTCTATAAGC ATAAAAATAT TAAGCAAAAC 
AGAGACATTT TGGTGAACTG GATAATCAAG ATTCATAATA AGTTTGGCCT TCTGCCGGAA 
ACTCTATATT TGGCCATAAA CATAATGGAT AGATTTCTTT GTGAAGAGGT GGTTCAGTTA 
AATAGATTGC AACTGGTTGG TACATCATGC CTGTTCATCG CATCTAAGTA TGAGGAAATA 
TACTCCCCTA GCATAAAACA TTTTGCGTAC GAGACAGACG GTGCATGCTC TGTGGAAGAT 
ATTAAAGAGG GTGAAAGATT TATATTGGAA AAACTCGATT TTCAAATTAG TTTCGCTAAC 
CCAATGAATT TCTTAAGGAG AATATCAAAA GCGGATGACT ATGATATCCA GTCTAGGACG 
TTAGCGAAGT TCCTAATGGA AATATCTATA GTGGACTTCA AGTTCATCGG GATATTACCG 
TCATTATGTG CCTCAGCGGC AATGTTCCTT TCGAGAAAAA TGCTGGGCAA GGGCACCTGG 
GATGGCAACT TAATCCATTA TAGTGGTGGT TACACAAAGG CAAAACTATA TCCCGTTTGC 
CAATTACTGA TGGATTATCT TGTTGGATCT ACTATTCACG ACGAGTTCTT GAAAAAATAT 
CAATCGAGAA GATTTTTAAA GGCTTCCATA ATTTCTATCG AATGGGCATT GAAGGTAAGG 
AAAAATGGAT ATGATATTAT GACATTGCAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011622.1
CDS	1..1416
Translation	MSRSLLVENSRTINSNEEKGVNESQYILQKRNVPRTILGNVTNNANILQEISMNRKIGMKNFSKLNNFFPLKDDVSRADDFTSSFNDSRQGVKQEVLNNKENIPEYGYSEQEKQQCSNDDSFHTNSTALSCNRLIYSENKSISTQMEWQKKIMREDSKKKRPISTLVEQDDQKKFKLHELTTEEEVLEEYEWDDLDEEDCDDPLMVSEEVNDIFDYLHHLEIITLPNKANLYKHKNIKQNRDILVNWIIKIHNKFGLLPETLYLAINIMDRFLCEEVVQLNRLQLVGTSCLFIASKYEEIYSPSIKHFAYETDGACSVEDIKEGERFILEKLDFQISFANPMNFLRRISKADDYDIQSRTLAKFLMEISIVDFKFIGILPSLCASAAMFLSRKMLGKGTWDGNLIHYSGGYTKAKLYPVCQLLMDYLVGSTIHDEFLKKYQSRRFLKASIISIEWALKVRKNGYDIMTLHE

Target ORF information:

RefSeq Version	NM_001181237.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C B-type cyclin CLB1 (CLB1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181237.1

ORF Insert Sequence:

ATGTCACGAT CCCTTTTGGT AGAGAATAGT AGAACCATTA ATAGTAATGA AGAGAAGGGG 
GTAAACGAAA GTCAATATAT CTTACAAAAG AGAAACGTCC CAAGGACCAT TCTCGGTAAT 
GTCACAAATA ATGCAAATAT CCTGCAAGAG ATTTCTATGA ACAGAAAAAT TGGGATGAAG 
AACTTCTCAA AATTGAACAA CTTCTTTCCT TTAAAGGATG ATGTTTCGAG AGCCGATGAC 
TTCACCTCCT CTTTTAATGA CAGTAGACAG GGGGTGAAAC AAGAAGTACT AAACAATAAG 
GAAAATATTC CTGAATATGG ATATTCCGAG CAAGAAAAGC AGCAGTGCTC AAATGATGAC 
TCTTTTCATA CGAATTCGAC TGCCTTGAGC TGTAATCGGT TGATATACTC TGAGAACAAG 
TCTATTTCCA CTCAAATGGA ATGGCAGAAA AAAATAATGA GAGAAGATTC CAAAAAAAAA 
AGGCCAATAT CAACTCTTGT AGAGCAGGAT GACCAGAAAA AGTTCAAACT CCATGAATTG 
ACTACAGAAG AAGAGGTTCT GGAGGAGTAC GAATGGGATG ACCTAGATGA AGAAGATTGT 
GATGATCCCT TAATGGTGAG CGAAGAGGTT AATGATATCT TCGATTATTT GCATCACTTG 
GAAATAATCA CATTGCCAAA TAAGGCGAAT CTCTATAAGC ATAAAAATAT TAAGCAAAAC 
AGAGACATTT TGGTGAACTG GATAATCAAG ATTCATAATA AGTTTGGCCT TCTGCCGGAA 
ACTCTATATT TGGCCATAAA CATAATGGAT AGATTTCTTT GTGAAGAGGT GGTTCAGTTA 
AATAGATTGC AACTGGTTGG TACATCATGC CTGTTCATCG CATCTAAGTA TGAGGAAATA 
TACTCCCCTA GCATAAAACA TTTTGCGTAC GAGACAGACG GTGCATGCTC TGTGGAAGAT 
ATTAAAGAGG GTGAAAGATT TATATTGGAA AAACTCGATT TTCAAATTAG TTTCGCTAAC 
CCAATGAATT TCTTAAGGAG AATATCAAAA GCGGATGACT ATGATATCCA GTCTAGGACG 
TTAGCGAAGT TCCTAATGGA AATATCTATA GTGGACTTCA AGTTCATCGG GATATTACCG 
TCATTATGTG CCTCAGCGGC AATGTTCCTT TCGAGAAAAA TGCTGGGCAA GGGCACCTGG 
GATGGCAACT TAATCCATTA TAGTGGTGGT TACACAAAGG CAAAACTATA TCCCGTTTGC 
CAATTACTGA TGGATTATCT TGTTGGATCT ACTATTCACG ACGAGTTCTT GAAAAAATAT 
CAATCGAGAA GATTTTTAAA GGCTTCCATA ATTTCTATCG AATGGGCATT GAAGGTAAGG 
AAAAATGGAT ATGATATTAT GACATTGCAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CLB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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