ESP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ESP1
Entrez Gene ID	852990
Full Name	separase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181227.3	NP_011612.3	separase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	ESP1	NP_011612.3
Kluyveromyces lactis	KLLA0D15136g	XP_453732.1
Eremothecium gossypii	AGOS_AAR089C	NP_982630.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

crystal structures at up to 2.6 A resolution of the yeast Saccharomyces cerevisiae separase-securin complex
Title: Molecular mechanism for the regulation of yeast separase by securin.

We propose that Esp1 serves two roles to mediate Ty1 transposition - one to remove cohesin and the second to target Ty1-IN to chromatin
Title: A role for the budding yeast separase, Esp1, in Ty1 element retrotransposition.

Separase acts directly on Scc1 and also indirectly, through inhibition of PP2A(Cdc55), to stimulate cohesin cleavage, providing a feedforward loop that may contribute to a robust and timely anaphase.
Title: Separase biosensor reveals that cohesin cleavage timing depends on phosphatase PP2A(Cdc55) regulation.

phosphorylation either of separase or cohesin may be necessary for Rec8 cleavage
Title: Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis.

Sgo1 can inhibit separase activity independently of securin.
Title: Shugoshin prevents cohesin cleavage by PP2A(Cdc55)-dependent inhibition of separase.

The following ESP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02324	NM_001181227.3 Latest version!	Saccharomyces cerevisiae S288C separase (ESP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESP1 ( NM_001181227.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011612.3 Saccharomyces cerevisiae S288C separase (ESP1), partial mRNA.

Price & Availability↑

CloneID	OSi02324
Clone ID Related Accession (Same CDS sequence)	NM_001181227.3
Accession Version	NM_001181227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	separase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181227.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATGGTAA AACAGGAGGA ACCGCTGAAT GAGATTAGTC CGAATACTCC GATGACTAGC 
AAGTCCTACC TTTTAAACGA CACGCTTTCG AAAGTACATC ATAGTGGCCA AACTAGACCA 
CTGACCTCGG TGTTGTCTGG AGACGCCTCA TCCAATTCTA TTGGCATACT AGCAATGCAT 
AATAACATAA TACGTGACTT TACAAAAATT GCAAGCAATA ACATAGACTT AGCCATTGAG 
GATATTACCA CTGTTGACCA TTCATTAAAT TCAATATATA GCCTATTGAA GAGCCATCAC 
ATGTGGGGCC ATATAAACTC CACTGTTAAA CAACATTTGA TGATAATTGT TAAACTAATA 
AATAATAACG CATTAGGATT GGCCTCCTCT GAAATTATTT TTCTTTTCAA TGAAACGAAT 
TTATTTCAAG CTCATAGTCT GAAAAACATT CTTCTAGCAG ACTTCAGTAC GTGGAACGAC 
TACTATCTTT CAAATCTGAA AATATTAGCG CTGCAGATTA TACTGAAAAG AAAGCTAGTA 
GACGAATACT TACCGCATAT ATTAGAACTA TTTTCCCATG ATAAAAGATA TCTTTTGAAA 
GACCCTAACC TCAAAGCTCA CGCTCTCACA AAAATCGTGC TGAGCTTTTT TTCTGTCACG 
ACGAGTTGCA AAGTTCTATT CGGGCTAAAA TTTCTACAAT ATATCAAACA GTTCAAATTA 
CCTTTTAAAA AGTTCATTAG CAACATTACC GTCGAGTGTT TTAGCAAAAA CCTTCTTCAT 
AAAAATTATT TGGAGATGGG ACCTAATAAA ATATATTTAA ATTCATTTTA CTTATCCTAT 
TCCATGCTGT ACGATGGATT GGATAAAATA ATGCTTCTAG ACATTTTAAG TTACGAAGAA 
ACTACTGAGG TACAAAGAGC GATAAAAAGT AAAAAAGAAT TCAATGAATA CTGCAACATG 
AGTGAAAACC GTCTTCTCTG GTCCTGTATA TCTGTCGATG ATTTGAACGT AATACTCGAA 
AATGCAACGA ATTTTCTACA AAATAAAGGG AAACATATAT CCGCAACATT AAAATGTTTA 
GTCTGTCTTT GGTCTACTAT TCGCCTAGAG GGATTACCTA AAAATAAGGA TATATTGAGG 
CAGTTTGATT GTACTGTTAT TTATATCAAC TCAAATATTA AATCCATTAA TGACGAATCT 
GCGGCGGCAT TATTATCCGA ACTTCTGGGA GTTTTATCAG AAATTTGTAT CGATTATAAA 
GAGCCTAAAC GACTGTCAAA TATTATCAGT GTCCTTTTCA ATGCTTCTGT TTTATTTAAG 
AGTCATTCGT TTTTGTTAAA GACTGCTAAT TTGGAAATCT CAAATGTTTT AATCAGTAAT 
GATTCAAAAA CATCTCATCG TACTATACTG AAATTTGAAA AATTTATATC TAGTGCTCAG 
AGTGCCCAGA AAAAAATTGA AATCTTTTCC TGTTTGTTCA ATGTATATTG CATGCTACGA 
AATGATACGC TATCATTTGT TTTTGACTTT TGCCAAAATG CATTCATTCA TTGCTTTACA 
CGCCTAAAAA TCACAAAATT CATCGAGTTT TCAAACAGTT CAGAAATTAT GCTTTCAGTA 
CTGTATGGAA ATTCATCTAT CGAAAACATT CCTTCCGAAA ATTGGTCACA ACTAAGCAGA 
ATGATATTTT GCTCACTGAG GGGTATCTTT GATTTGGATC CTTTAGAGCT AAATAATACG 
TTTGATAAAT TACACTTGCT CAACAAATAT GAGCTATTAA TACGAATAGT GTACCTATTG 
AACCTGGACA TGAGTAAACA CCTGACAACG AATCTATCGA AAATAACTAA GCTGTACATA 
AACAAATGGC TACAAAAATC AGATGAAAAG GCCGAGAGAA TATCGTCATT TGAAATGGAC 
TTCGTTAAAA TGCTATTGTG CTACTTGAAC TTTAATAATT TTGATAAGTT ATCCATTGAA 
CTTTCACTCT GTATTAAATC AAAAGAAAAG TATTACTCCA GTATTGTGCC TTATGCCGAC 
AATTATTTGC TAGAGGCTTA TCTGAGCTTA TATATGATTG ATGATGCCCT CATGATGAAG 
AATCAACTAC AAAAAACAAT GAATTTATCA ACGGCGAAAA TAGAGCAGGC GTTATTACAT 
GCAAGTTCTT TAATAAATGT CCACTTGTGG GACTCTGACT TGACAGCGTT TCAAATATAT 
TTTGGAAAAA CACTGCCTGC TATGAAGCCT GAATTATTTG ATATCAATAA CGACCACAAC 
CTACCCATGT CACTTTACAT CAAAGTCATA CTTTTGAACA TAAAAATTTT TAATGAAAGC 
GCGAAATTAA ATATTAAAGC TGGTAATGTT ATATCCGCAG TAATTGATTG TAGAAAAGCA 
CAAAATTTAG CATTATCGTT GCTCAAAAAG AAGAACAAAT TGTCACAAGG ATCCAGGCTA 
GCATTACTAA AAAGTCTATC GTTTAGTTTT TTCCAACTCA TTAAAATACA TATACGCATT 
GGTTCTGCCA GAGACTGTGA GTTCTACAGT AAAGAACTGT CTAGAATTAT TTCTGACTTA 
GAGGAACCTA TTATCGTCTA CAGATGTCTG CATTTTCTTC ACAGATACTA TATGATTACA 
GAGCAAACCT GTCTTCAAAA TATAACTTTG GGAAAAGCCA ATAAGGCATT TGATTATCTT 
GATGCTGAGG CAGACATTAC AAGTTTGACA ATGTTTCTGT ATGATAATAA GGAATTTGTA 
AAGTTAGAGC AGTCGTTGGT CCTATATTTC GGTGACCAGC TAGAAAAAAC ATTCCTACCT 
AATTTGTGGA AGCTTCACTT GGGTAAGGAT ATTGACGACA GCATTTGTCT GTCCGAATAT 
ATGCCCAAAA ATGTAATCAA TAGAGTACAC AATATGTGGC AAAAAGTAAT GAGCCAATTG 
GAGGAAGATC CTTTCTTCAA AGGTATGTTT GAATCTACCC TTGGGATTCC CTCTTCTCTG 
CCTGTTATTC CTAGCACGAT GCCCAACAAC ATTTTAAAAA CTCCTTCCAA GCATAGTACA 
GGATTGAAGC TTTGCGATTC ACCAAGATCG TCGAGCATGA CGCCTAGAGG TAAGAATATA 
AGACAGAAAT TTGATAGAAT TGCTGCTATT AGTAAATTAA AACAGATGAA AGAACTTTTG 
GAGAGTTTGA AACTAGACAC ATTAGATAAT CACGAACTGT CCAAAATTTC CTCCCTTTCT 
TCATTAACTC TGACTATCCT TTCAAACATA ACTAGTATCC ATAATGCTGA GAGTAGTTTG 
ATAACTAATT TTTCGCTAAC TGACTTGCCA AGGCATATGC CTTTGTTATT CGATAAGGTA 
TTGAATAATA TTGATAATAA AAATTACCGA GAATTTCGCG TTTCATCACT AATAGCTCCG 
AACAATATAT CCACAATTAC AGAGAGTATT AGAGTATCTG CCGCCCAGAA GGACTTAATG 
GAGAGCAACC TTAATATTAA TGTTATAACT ATTGACTTCT GTCCCATAAC TGGAAATTTA 
CTTCTATCCA AATTAGAACC GCGACGTAAG AGAAGGACGC ATTTAAGGCT ACCGCTAATA 
CGATCTAATT CAAGAGACCT CGACGAAGTT CATCTCTCAT TTCCGGAGGC TACAAAAAAA 
CTACTTTCTA TTATCAATGA AAGCAACCAA ACGACGTCAG TGGAAGTTAC CAACAAGATT 
AAAACAAGAG AAGAAAGAAA ATCTTGGTGG ACCACAAGAT ATGATTTGGA TAAAAGAATG 
CAACAATTGT TGAATAACAT TGAAAATAGT TGGTTCAATG GCGTTCAAGG GTTTTTTAGC 
CCTGAAGTAG TCGACAACTC GTTATTTGAA AAATTTAAAG ATAAATTTTA CGAAATTCTG 
CATCAAAATC TTCCTTCCAG AAAACTCTAT GGCAATCCGG CAATGTTTAT AAAAGTTGAA 
GATTGGGTAA TCGAATTGTT CTTGAAACTT AATCCGCAAG AAATCGATTT TCTTTCGAAG 
ATGGAAGACC TGATATACTT TGTTCTTGAT ATTCTTCTCT TCCATGGTGA AGAAAATGCT 
TACGACGAAA TTGATTTCAG CATGCTTCAC GTGCAGTTAG AAGAGCAAAT AAAGAAATAT 
AGGGCAACTA TGACAACTAA TAGTATATTT CATACATTTT TAGTCGTTAG CAGTTCATGC 
CATTTATTCC CTTGGGAGTG TCTTTCATTT TTGAAAGATT TGTCTATCAC ACGTGTTCCG 
TCCTATGTTT GTCTGAATAA ACTTTTATCG CGTTTCCATT ACCAACTACC CCTTCAAGTA 
ACTATTGAGG ACAACATTAG TATGATATTA AATCCGAACG GAGATTTGTC CAGGACTGAA 
TCTAAATTCA AGGGTATGTT CCAAAAAATA ATCGATGCAA AACCTTCCTC TCAATTAGTG 
ATGAACGAGA AGCCGGAAGA GGAAACACTT TTGAAAATGC TGCAAAATTC TAACTTATTC 
GTTTATATAG GGCATGGCGG AGGTGAACAA TATGTAAGGA GTAAGGAGAT TAAGAAATGC 
ACCAAAATTG CCCCCAGCTT TTTACTGGGC TGCTCTTCAG CAGCGATGAA ATATTACGGC 
AAGTTAGAAC CTACTGGAAC AATCTATACT TATTTGCTGG GAGGATGCCC TATGGTACTA 
GGTAATCTGT GGGATGTAAC TGACAAAGAT ATTGATAAAT TTAGCGAGGA ACTTTTTGAA 
AAGATGGGAT TTCGCTGTAA TACTGACGAT TTGAATGGTA ATAGCCTATC TGTTTCATAT 
GCCGTCTCTA AATCTAGAGG TGTATGCCAT CTACGTTACT TGAACGGCGC AGCTCCTGTT 
ATTTATGGGT TACCGATCAA GTTCGTATCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011612.3
CDS	1..4893
Translation	MMVKQEEPLNEISPNTPMTSKSYLLNDTLSKVHHSGQTRPLTSVLSGDASSNSIGILAMHNNIIRDFTKIASNNIDLAIEDITTVDHSLNSIYSLLKSHHMWGHINSTVKQHLMIIVKLINNNALGLASSEIIFLFNETNLFQAHSLKNILLADFSTWNDYYLSNLKILALQIILKRKLVDEYLPHILELFSHDKRYLLKDPNLKAHALTKIVLSFFSVTTSCKVLFGLKFLQYIKQFKLPFKKFISNITVECFSKNLLHKNYLEMGPNKIYLNSFYLSYSMLYDGLDKIMLLDILSYEETTEVQRAIKSKKEFNEYCNMSENRLLWSCISVDDLNVILENATNFLQNKGKHISATLKCLVCLWSTIRLEGLPKNKDILRQFDCTVIYINSNIKSINDESAAALLSELLGVLSEICIDYKEPKRLSNIISVLFNASVLFKSHSFLLKTANLEISNVLISNDSKTSHRTILKFEKFISSAQSAQKKIEIFSCLFNVYCMLRNDTLSFVFDFCQNAFIHCFTRLKITKFIEFSNSSEIMLSVLYGNSSIENIPSENWSQLSRMIFCSLRGIFDLDPLELNNTFDKLHLLNKYELLIRIVYLLNLDMSKHLTTNLSKITKLYINKWLQKSDEKAERISSFEMDFVKMLLCYLNFNNFDKLSIELSLCIKSKEKYYSSIVPYADNYLLEAYLSLYMIDDALMMKNQLQKTMNLSTAKIEQALLHASSLINVHLWDSDLTAFQIYFGKTLPAMKPELFDINNDHNLPMSLYIKVILLNIKIFNESAKLNIKAGNVISAVIDCRKAQNLALSLLKKKNKLSQGSRLALLKSLSFSFFQLIKIHIRIGSARDCEFYSKELSRIISDLEEPIIVYRCLHFLHRYYMITEQTCLQNITLGKANKAFDYLDAEADITSLTMFLYDNKEFVKLEQSLVLYFGDQLEKTFLPNLWKLHLGKDIDDSICLSEYMPKNVINRVHNMWQKVMSQLEEDPFFKGMFESTLGIPSSLPVIPSTMPNNILKTPSKHSTGLKLCDSPRSSSMTPRGKNIRQKFDRIAAISKLKQMKELLESLKLDTLDNHELSKISSLSSLTLTILSNITSIHNAESSLITNFSLTDLPRHMPLLFDKVLNNIDNKNYREFRVSSLIAPNNISTITESIRVSAAQKDLMESNLNINVITIDFCPITGNLLLSKLEPRRKRRTHLRLPLIRSNSRDLDEVHLSFPEATKKLLSIINESNQTTSVEVTNKIKTREERKSWWTTRYDLDKRMQQLLNNIENSWFNGVQGFFSPEVVDNSLFEKFKDKFYEILHQNLPSRKLYGNPAMFIKVEDWVIELFLKLNPQEIDFLSKMEDLIYFVLDILLFHGEENAYDEIDFSMLHVQLEEQIKKYRATMTTNSIFHTFLVVSSSCHLFPWECLSFLKDLSITRVPSYVCLNKLLSRFHYQLPLQVTIEDNISMILNPNGDLSRTESKFKGMFQKIIDAKPSSQLVMNEKPEEETLLKMLQNSNLFVYIGHGGGEQYVRSKEIKKCTKIAPSFLLGCSSAAMKYYGKLEPTGTIYTYLLGGCPMVLGNLWDVTDKDIDKFSEELFEKMGFRCNTDDLNGNSLSVSYAVSKSRGVCHLRYLNGAAPVIYGLPIKFVS

Target ORF information:

RefSeq Version	NM_001181227.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C separase (ESP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181227.3

ORF Insert Sequence:

ATGATGGTAA AACAGGAGGA ACCGCTGAAT GAGATTAGTC CGAATACTCC GATGACTAGC 
AAGTCCTACC TTTTAAACGA CACGCTTTCG AAAGTACATC ATAGTGGCCA AACTAGACCA 
CTGACCTCGG TGTTGTCTGG AGACGCCTCA TCCAATTCTA TTGGCATACT AGCAATGCAT 
AATAACATAA TACGTGACTT TACAAAAATT GCAAGCAATA ACATAGACTT AGCCATTGAG 
GATATTACCA CTGTTGACCA TTCATTAAAT TCAATATATA GCCTATTGAA GAGCCATCAC 
ATGTGGGGCC ATATAAACTC CACTGTTAAA CAACATTTGA TGATAATTGT TAAACTAATA 
AATAATAACG CATTAGGATT GGCCTCCTCT GAAATTATTT TTCTTTTCAA TGAAACGAAT 
TTATTTCAAG CTCATAGTCT GAAAAACATT CTTCTAGCAG ACTTCAGTAC GTGGAACGAC 
TACTATCTTT CAAATCTGAA AATATTAGCG CTGCAGATTA TACTGAAAAG AAAGCTAGTA 
GACGAATACT TACCGCATAT ATTAGAACTA TTTTCCCATG ATAAAAGATA TCTTTTGAAA 
GACCCTAACC TCAAAGCTCA CGCTCTCACA AAAATCGTGC TGAGCTTTTT TTCTGTCACG 
ACGAGTTGCA AAGTTCTATT CGGGCTAAAA TTTCTACAAT ATATCAAACA GTTCAAATTA 
CCTTTTAAAA AGTTCATTAG CAACATTACC GTCGAGTGTT TTAGCAAAAA CCTTCTTCAT 
AAAAATTATT TGGAGATGGG ACCTAATAAA ATATATTTAA ATTCATTTTA CTTATCCTAT 
TCCATGCTGT ACGATGGATT GGATAAAATA ATGCTTCTAG ACATTTTAAG TTACGAAGAA 
ACTACTGAGG TACAAAGAGC GATAAAAAGT AAAAAAGAAT TCAATGAATA CTGCAACATG 
AGTGAAAACC GTCTTCTCTG GTCCTGTATA TCTGTCGATG ATTTGAACGT AATACTCGAA 
AATGCAACGA ATTTTCTACA AAATAAAGGG AAACATATAT CCGCAACATT AAAATGTTTA 
GTCTGTCTTT GGTCTACTAT TCGCCTAGAG GGATTACCTA AAAATAAGGA TATATTGAGG 
CAGTTTGATT GTACTGTTAT TTATATCAAC TCAAATATTA AATCCATTAA TGACGAATCT 
GCGGCGGCAT TATTATCCGA ACTTCTGGGA GTTTTATCAG AAATTTGTAT CGATTATAAA 
GAGCCTAAAC GACTGTCAAA TATTATCAGT GTCCTTTTCA ATGCTTCTGT TTTATTTAAG 
AGTCATTCGT TTTTGTTAAA GACTGCTAAT TTGGAAATCT CAAATGTTTT AATCAGTAAT 
GATTCAAAAA CATCTCATCG TACTATACTG AAATTTGAAA AATTTATATC TAGTGCTCAG 
AGTGCCCAGA AAAAAATTGA AATCTTTTCC TGTTTGTTCA ATGTATATTG CATGCTACGA 
AATGATACGC TATCATTTGT TTTTGACTTT TGCCAAAATG CATTCATTCA TTGCTTTACA 
CGCCTAAAAA TCACAAAATT CATCGAGTTT TCAAACAGTT CAGAAATTAT GCTTTCAGTA 
CTGTATGGAA ATTCATCTAT CGAAAACATT CCTTCCGAAA ATTGGTCACA ACTAAGCAGA 
ATGATATTTT GCTCACTGAG GGGTATCTTT GATTTGGATC CTTTAGAGCT AAATAATACG 
TTTGATAAAT TACACTTGCT CAACAAATAT GAGCTATTAA TACGAATAGT GTACCTATTG 
AACCTGGACA TGAGTAAACA CCTGACAACG AATCTATCGA AAATAACTAA GCTGTACATA 
AACAAATGGC TACAAAAATC AGATGAAAAG GCCGAGAGAA TATCGTCATT TGAAATGGAC 
TTCGTTAAAA TGCTATTGTG CTACTTGAAC TTTAATAATT TTGATAAGTT ATCCATTGAA 
CTTTCACTCT GTATTAAATC AAAAGAAAAG TATTACTCCA GTATTGTGCC TTATGCCGAC 
AATTATTTGC TAGAGGCTTA TCTGAGCTTA TATATGATTG ATGATGCCCT CATGATGAAG 
AATCAACTAC AAAAAACAAT GAATTTATCA ACGGCGAAAA TAGAGCAGGC GTTATTACAT 
GCAAGTTCTT TAATAAATGT CCACTTGTGG GACTCTGACT TGACAGCGTT TCAAATATAT 
TTTGGAAAAA CACTGCCTGC TATGAAGCCT GAATTATTTG ATATCAATAA CGACCACAAC 
CTACCCATGT CACTTTACAT CAAAGTCATA CTTTTGAACA TAAAAATTTT TAATGAAAGC 
GCGAAATTAA ATATTAAAGC TGGTAATGTT ATATCCGCAG TAATTGATTG TAGAAAAGCA 
CAAAATTTAG CATTATCGTT GCTCAAAAAG AAGAACAAAT TGTCACAAGG ATCCAGGCTA 
GCATTACTAA AAAGTCTATC GTTTAGTTTT TTCCAACTCA TTAAAATACA TATACGCATT 
GGTTCTGCCA GAGACTGTGA GTTCTACAGT AAAGAACTGT CTAGAATTAT TTCTGACTTA 
GAGGAACCTA TTATCGTCTA CAGATGTCTG CATTTTCTTC ACAGATACTA TATGATTACA 
GAGCAAACCT GTCTTCAAAA TATAACTTTG GGAAAAGCCA ATAAGGCATT TGATTATCTT 
GATGCTGAGG CAGACATTAC AAGTTTGACA ATGTTTCTGT ATGATAATAA GGAATTTGTA 
AAGTTAGAGC AGTCGTTGGT CCTATATTTC GGTGACCAGC TAGAAAAAAC ATTCCTACCT 
AATTTGTGGA AGCTTCACTT GGGTAAGGAT ATTGACGACA GCATTTGTCT GTCCGAATAT 
ATGCCCAAAA ATGTAATCAA TAGAGTACAC AATATGTGGC AAAAAGTAAT GAGCCAATTG 
GAGGAAGATC CTTTCTTCAA AGGTATGTTT GAATCTACCC TTGGGATTCC CTCTTCTCTG 
CCTGTTATTC CTAGCACGAT GCCCAACAAC ATTTTAAAAA CTCCTTCCAA GCATAGTACA 
GGATTGAAGC TTTGCGATTC ACCAAGATCG TCGAGCATGA CGCCTAGAGG TAAGAATATA 
AGACAGAAAT TTGATAGAAT TGCTGCTATT AGTAAATTAA AACAGATGAA AGAACTTTTG 
GAGAGTTTGA AACTAGACAC ATTAGATAAT CACGAACTGT CCAAAATTTC CTCCCTTTCT 
TCATTAACTC TGACTATCCT TTCAAACATA ACTAGTATCC ATAATGCTGA GAGTAGTTTG 
ATAACTAATT TTTCGCTAAC TGACTTGCCA AGGCATATGC CTTTGTTATT CGATAAGGTA 
TTGAATAATA TTGATAATAA AAATTACCGA GAATTTCGCG TTTCATCACT AATAGCTCCG 
AACAATATAT CCACAATTAC AGAGAGTATT AGAGTATCTG CCGCCCAGAA GGACTTAATG 
GAGAGCAACC TTAATATTAA TGTTATAACT ATTGACTTCT GTCCCATAAC TGGAAATTTA 
CTTCTATCCA AATTAGAACC GCGACGTAAG AGAAGGACGC ATTTAAGGCT ACCGCTAATA 
CGATCTAATT CAAGAGACCT CGACGAAGTT CATCTCTCAT TTCCGGAGGC TACAAAAAAA 
CTACTTTCTA TTATCAATGA AAGCAACCAA ACGACGTCAG TGGAAGTTAC CAACAAGATT 
AAAACAAGAG AAGAAAGAAA ATCTTGGTGG ACCACAAGAT ATGATTTGGA TAAAAGAATG 
CAACAATTGT TGAATAACAT TGAAAATAGT TGGTTCAATG GCGTTCAAGG GTTTTTTAGC 
CCTGAAGTAG TCGACAACTC GTTATTTGAA AAATTTAAAG ATAAATTTTA CGAAATTCTG 
CATCAAAATC TTCCTTCCAG AAAACTCTAT GGCAATCCGG CAATGTTTAT AAAAGTTGAA 
GATTGGGTAA TCGAATTGTT CTTGAAACTT AATCCGCAAG AAATCGATTT TCTTTCGAAG 
ATGGAAGACC TGATATACTT TGTTCTTGAT ATTCTTCTCT TCCATGGTGA AGAAAATGCT 
TACGACGAAA TTGATTTCAG CATGCTTCAC GTGCAGTTAG AAGAGCAAAT AAAGAAATAT 
AGGGCAACTA TGACAACTAA TAGTATATTT CATACATTTT TAGTCGTTAG CAGTTCATGC 
CATTTATTCC CTTGGGAGTG TCTTTCATTT TTGAAAGATT TGTCTATCAC ACGTGTTCCG 
TCCTATGTTT GTCTGAATAA ACTTTTATCG CGTTTCCATT ACCAACTACC CCTTCAAGTA 
ACTATTGAGG ACAACATTAG TATGATATTA AATCCGAACG GAGATTTGTC CAGGACTGAA 
TCTAAATTCA AGGGTATGTT CCAAAAAATA ATCGATGCAA AACCTTCCTC TCAATTAGTG 
ATGAACGAGA AGCCGGAAGA GGAAACACTT TTGAAAATGC TGCAAAATTC TAACTTATTC 
GTTTATATAG GGCATGGCGG AGGTGAACAA TATGTAAGGA GTAAGGAGAT TAAGAAATGC 
ACCAAAATTG CCCCCAGCTT TTTACTGGGC TGCTCTTCAG CAGCGATGAA ATATTACGGC 
AAGTTAGAAC CTACTGGAAC AATCTATACT TATTTGCTGG GAGGATGCCC TATGGTACTA 
GGTAATCTGT GGGATGTAAC TGACAAAGAT ATTGATAAAT TTAGCGAGGA ACTTTTTGAA 
AAGATGGGAT TTCGCTGTAA TACTGACGAT TTGAATGGTA ATAGCCTATC TGTTTCATAT 
GCCGTCTCTA AATCTAGAGG TGTATGCCAT CTACGTTACT TGAACGGCGC AGCTCCTGTT 
ATTTATGGGT TACCGATCAA GTTCGTATCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ESP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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