ORF » Species Summary » Saccharomyces cerevisiae S288C » VAS1 cDNA ORF clone

VAS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following VAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02320 NM_001181223.1
Latest version!		 Saccharomyces cerevisiae S288C valine--tRNA ligase (VAS1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    VAS1 ( NM_001181223.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011608.1 Saccharomyces cerevisiae S288C valine--tRNA ligase (VAS1), partial mRNA.
    Price & Availability↑
    CloneID OSi02320
    Clone ID Related Accession (Same CDS sequence) NM_001181223.1
    Accession Version NM_001181223.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product valine--tRNA ligase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATAAGT GGTTAAACAC ATTATCTAAG ACATTCACTT TTCGGCTTTT GAACTGTCAT 
    TATAGGCGAT CATTACCACT TTGTCAAAAC TTTTCTCTGA AGAAGTCGTT AACTCATAAT 
    CAAGTCAGGT TCTTTAAAAT GAGCGATCTT GATAATTTGC CTCCAGTTGA CCCAAAGACT 
    GGTGAGGTCA TCATTAATCC GTTAAAGGAA GATGGCTCTC CAAAGACTCC TAAGGAAATT 
    GAAAAAGAGA AGAAAAAGGC TGAAAAACTG TTAAAGTTCG CTGCCAAACA AGCTAAAAAA 
    AATGCTGCTG CCACCACAGG TGCATCTCAA AAGAAACCTA AGAAAAAGAA GGAAGTTGAG 
    CCAATCCCTG AATTTATTGA CAAAACTGTT CCAGGTGAGA AAAAAATCTT AGTATCCTTG 
    GATGATCCGG CTTTAAAAGC TTATAACCCT GCTAACGTTG AAAGTTCTTG GTATGACTGG 
    TGGATCAAGA CTGGTGTTTT TGAACCTGAG TTTACCGCTG ATGGTAAGGT TAAACCAGAA 
    GGTGTATTTT GCATTCCAGC ACCTCCACCA AACGTCACTG GTGCCTTACA TATTGGTCAT 
    GCTTTGACTA TTGCTATCCA AGATTCTTTG ATCAGATATA ACAGAATGAA AGGTAAAACT 
    GTCTTATTCT TGCCAGGTTT CGACCATGCT GGTATTGCTA CTCAGTCCGT TGTGGAGAAG 
    CAAATCTGGG CTAAGGACAG AAAGACTAGA CATGACTATG GAAGAGAAGC TTTTGTTGGT 
    AAGGTCTGGG AATGGAAAGA GGAATACCAT AGCAGAATTA AGAACCAAAT TCAAAAATTG 
    GGGGCTTCTT ATGATTGGAG CCGCGAAGCT TTCACTTTGA GTCCAGAATT GACCAAGTCT 
    GTTGAAGAAG CTTTTGTTAG ACTACATGAT GAAGGTGTTA TTTATCGTGC GTCCAGATTA 
    GTTAATTGGT CTGTTAAATT GAATACCGCT ATCTCTAATT TGGAAGTCGA AAATAAGGAC 
    GTTAAAAGTA GAACGCTTTT ATCAGTCCCA GGCTATGATG AAAAGGTTGA ATTTGGTGTT 
    TTAACATCAT TTGCTTATCC AGTTATCGGT AGCGATGAAA AACTGATCAT TGCTACAACT 
    AGACCTGAAA CTATATTTGG TGATACTGCC GTTGCAGTTC ATCCTGATGA TGACCGTTAC 
    AAACACTTGC ATGGTAAGTT CATCCAACAT CCTTTCTTAC CAAGAAAAAT TCCAATTATC 
    ACCGACAAGG AAGCTGTTGA CATGGAATTC GGTACTGGTG CCGTTAAGAT CACTCCAGCC 
    CATGACCAAA ACGATTACAA TACCGGTAAG CGTCACAATT TGGAATTCAT CAATATTTTG 
    ACTGACGATG GTTTATTAAA CGAGGAGTGT GGTCCAGAGT GGCAAGGCAT GAAGAGGTTT 
    GATGCCAGAA AGAAGGTCAT TGAGCAGCTG AAGGAAAAGA ACCTATACGT TGGCCAAGAA 
    GATAATGAAA TGACCATTCC AACTTGTTCC AGATCTGGTG ACATTATTGA ACCTTTATTG 
    AAACCTCAAT GGTGGGTTTC TCAAAGTGAA ATGGCCAAAG ATGCTATTAA GGTTGTTAGG 
    GATGGTCAAA TTACCATCAC CCCCAAATCT TCTGAGGCTG AATATTTCCA TTGGTTGGGT 
    AACATCCAAG ATTGGTGTAT TTCCAGACAA TTATGGTGGG GTCATCGTTG TCCAGTTTAC 
    TTTATTAATA TCGAAGGCGA AGAACACGAT AGAATTGATG GTGACTATTG GGTTGCTGGT 
    AGGAGCATGG AGGAAGCTGA AAAGAAGGCT GCTGCCAAAT ACCCTAATTC CAAATTTACT 
    CTGGAACAAG ATGAAGATGT TTTAGACACC TGGTTCTCGT CCGGTTTGTG GCCTTTCTCC 
    ACTTTGGGTT GGCCAGAGAA GACTAAAGAC ATGGAAACTT TTTACCCCTT TTCTATGTTG 
    GAAACTGGTT GGGATATTCT TTTCTTCTGG GTTACTAGAA TGATTCTATT GGGCTTAAAA 
    TTGACCGGTT CAGTTCCATT CAAGGAAGTT TTCTGCCACT CTTTAGTCCG TGACGCTCAA 
    GGTCGTAAGA TGTCTAAATC TTTAGGTAAT GTTATTGACC CACTAGACGT TATTACTGGT 
    ATTAAGTTGG ATGATTTGCA TGCAAAATTA TTACAAGGTA ACTTAGATCC AAGAGAAGTT 
    GAAAAAGCTA AGATCGGTCA AAAGGAATCC TACCCTAACG GTATTCCTCA ATGTGGTACC 
    GATGCTATGA GGTTTGCATT ATGTGCTTAT ACCACTGGTG GTCGTGATAT TAACTTAGAT 
    ATCTTACGTG TCGAAGGTTA CAGAAAGTTC TGTAACAAAA TCTACCAAGC TACCAAGTTT 
    GCATTGATGA GACTCGGTGA CGATTATCAA CCACCTGCCA CTGAAGGTCT ATCAGGTAAC 
    GAATCCTTGG TTGAAAAATG GATCTTGCAC AAGCTGACTG AAACCTCGAA AATTGTCAAT 
    GAAGCTCTAG ATAAACGTGA CTTCTTGACG TCCACTAGCA GTATTTACGA ATTCTGGTAT 
    TTGATTTGTG ATGTTTACAT CGAGAACTCT AAATACTTGA TTCAAGAAGG CTCTGCTATT 
    GAAAAGAAGT CCGCAAAGGA TACATTGTAT ATCTTGCTGG ACAACGCTTT GAAATTAATC 
    CATCCATTCA TGCCATTCAT TTCTGAAGAA ATGTGGCAAA GACTTCCAAA GCGTTCCACT 
    GAGAAGGCTG CCTCAATTGT AAAAGCTTCT TATCCAGTTT ACGTATCTGA GTACGATGAT 
    GTCAAATCGG CCAATGCTTA CGACTTGGTC TTGAACATTA CCAAAGAAGC TCGTTCCTTG 
    TTATCTGAGT ACAATATTTT GAAGAATGGT AAGGTTTTCG TTGAATCTAA CCACGAGGAA 
    TACTTCAAAA CTGCTGAAGA TCAGAAAGAT TCTATTGTCT CGTTGATCAA GGCCATCGAC 
    GAAGTCACTG TTGTTCGTGA TGCTTCCGAA ATTCCAGAAG GTTGCGTATT GCAATCTGTT 
    AACCCAGAAG TCAATGTACA TCTTCTCGTC AAGGGACACG TTGATATTGA TGCTGAAATT 
    GCGAAAGTTC AAAAGAAACT TGAAAAGGCT AAAAAATCCA AGAACGGTAT TGAACAAACC 
    ATTAACAGTA AGGATTACGA AACAAAGGCT AATACACAGG CCAAGGAAGC CAATAAAAGC 
    AAGCTGGATA ACACTGTTGC CGAAATCGAA GGTTTGGAAG CTACTATTGA AAACTTGAAG 
    CGTTTGAAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011608.1
    CDS1..3315
    Translation

    Target ORF information:

    RefSeq Version NM_001181223.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C valine--tRNA ligase (VAS1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATAAGT GGTTAAACAC ATTATCTAAG ACATTCACTT TTCGGCTTTT GAACTGTCAT 
    TATAGGCGAT CATTACCACT TTGTCAAAAC TTTTCTCTGA AGAAGTCGTT AACTCATAAT 
    CAAGTCAGGT TCTTTAAAAT GAGCGATCTT GATAATTTGC CTCCAGTTGA CCCAAAGACT 
    GGTGAGGTCA TCATTAATCC GTTAAAGGAA GATGGCTCTC CAAAGACTCC TAAGGAAATT 
    GAAAAAGAGA AGAAAAAGGC TGAAAAACTG TTAAAGTTCG CTGCCAAACA AGCTAAAAAA 
    AATGCTGCTG CCACCACAGG TGCATCTCAA AAGAAACCTA AGAAAAAGAA GGAAGTTGAG 
    CCAATCCCTG AATTTATTGA CAAAACTGTT CCAGGTGAGA AAAAAATCTT AGTATCCTTG 
    GATGATCCGG CTTTAAAAGC TTATAACCCT GCTAACGTTG AAAGTTCTTG GTATGACTGG 
    TGGATCAAGA CTGGTGTTTT TGAACCTGAG TTTACCGCTG ATGGTAAGGT TAAACCAGAA 
    GGTGTATTTT GCATTCCAGC ACCTCCACCA AACGTCACTG GTGCCTTACA TATTGGTCAT 
    GCTTTGACTA TTGCTATCCA AGATTCTTTG ATCAGATATA ACAGAATGAA AGGTAAAACT 
    GTCTTATTCT TGCCAGGTTT CGACCATGCT GGTATTGCTA CTCAGTCCGT TGTGGAGAAG 
    CAAATCTGGG CTAAGGACAG AAAGACTAGA CATGACTATG GAAGAGAAGC TTTTGTTGGT 
    AAGGTCTGGG AATGGAAAGA GGAATACCAT AGCAGAATTA AGAACCAAAT TCAAAAATTG 
    GGGGCTTCTT ATGATTGGAG CCGCGAAGCT TTCACTTTGA GTCCAGAATT GACCAAGTCT 
    GTTGAAGAAG CTTTTGTTAG ACTACATGAT GAAGGTGTTA TTTATCGTGC GTCCAGATTA 
    GTTAATTGGT CTGTTAAATT GAATACCGCT ATCTCTAATT TGGAAGTCGA AAATAAGGAC 
    GTTAAAAGTA GAACGCTTTT ATCAGTCCCA GGCTATGATG AAAAGGTTGA ATTTGGTGTT 
    TTAACATCAT TTGCTTATCC AGTTATCGGT AGCGATGAAA AACTGATCAT TGCTACAACT 
    AGACCTGAAA CTATATTTGG TGATACTGCC GTTGCAGTTC ATCCTGATGA TGACCGTTAC 
    AAACACTTGC ATGGTAAGTT CATCCAACAT CCTTTCTTAC CAAGAAAAAT TCCAATTATC 
    ACCGACAAGG AAGCTGTTGA CATGGAATTC GGTACTGGTG CCGTTAAGAT CACTCCAGCC 
    CATGACCAAA ACGATTACAA TACCGGTAAG CGTCACAATT TGGAATTCAT CAATATTTTG 
    ACTGACGATG GTTTATTAAA CGAGGAGTGT GGTCCAGAGT GGCAAGGCAT GAAGAGGTTT 
    GATGCCAGAA AGAAGGTCAT TGAGCAGCTG AAGGAAAAGA ACCTATACGT TGGCCAAGAA 
    GATAATGAAA TGACCATTCC AACTTGTTCC AGATCTGGTG ACATTATTGA ACCTTTATTG 
    AAACCTCAAT GGTGGGTTTC TCAAAGTGAA ATGGCCAAAG ATGCTATTAA GGTTGTTAGG 
    GATGGTCAAA TTACCATCAC CCCCAAATCT TCTGAGGCTG AATATTTCCA TTGGTTGGGT 
    AACATCCAAG ATTGGTGTAT TTCCAGACAA TTATGGTGGG GTCATCGTTG TCCAGTTTAC 
    TTTATTAATA TCGAAGGCGA AGAACACGAT AGAATTGATG GTGACTATTG GGTTGCTGGT 
    AGGAGCATGG AGGAAGCTGA AAAGAAGGCT GCTGCCAAAT ACCCTAATTC CAAATTTACT 
    CTGGAACAAG ATGAAGATGT TTTAGACACC TGGTTCTCGT CCGGTTTGTG GCCTTTCTCC 
    ACTTTGGGTT GGCCAGAGAA GACTAAAGAC ATGGAAACTT TTTACCCCTT TTCTATGTTG 
    GAAACTGGTT GGGATATTCT TTTCTTCTGG GTTACTAGAA TGATTCTATT GGGCTTAAAA 
    TTGACCGGTT CAGTTCCATT CAAGGAAGTT TTCTGCCACT CTTTAGTCCG TGACGCTCAA 
    GGTCGTAAGA TGTCTAAATC TTTAGGTAAT GTTATTGACC CACTAGACGT TATTACTGGT 
    ATTAAGTTGG ATGATTTGCA TGCAAAATTA TTACAAGGTA ACTTAGATCC AAGAGAAGTT 
    GAAAAAGCTA AGATCGGTCA AAAGGAATCC TACCCTAACG GTATTCCTCA ATGTGGTACC 
    GATGCTATGA GGTTTGCATT ATGTGCTTAT ACCACTGGTG GTCGTGATAT TAACTTAGAT 
    ATCTTACGTG TCGAAGGTTA CAGAAAGTTC TGTAACAAAA TCTACCAAGC TACCAAGTTT 
    GCATTGATGA GACTCGGTGA CGATTATCAA CCACCTGCCA CTGAAGGTCT ATCAGGTAAC 
    GAATCCTTGG TTGAAAAATG GATCTTGCAC AAGCTGACTG AAACCTCGAA AATTGTCAAT 
    GAAGCTCTAG ATAAACGTGA CTTCTTGACG TCCACTAGCA GTATTTACGA ATTCTGGTAT 
    TTGATTTGTG ATGTTTACAT CGAGAACTCT AAATACTTGA TTCAAGAAGG CTCTGCTATT 
    GAAAAGAAGT CCGCAAAGGA TACATTGTAT ATCTTGCTGG ACAACGCTTT GAAATTAATC 
    CATCCATTCA TGCCATTCAT TTCTGAAGAA ATGTGGCAAA GACTTCCAAA GCGTTCCACT 
    GAGAAGGCTG CCTCAATTGT AAAAGCTTCT TATCCAGTTT ACGTATCTGA GTACGATGAT 
    GTCAAATCGG CCAATGCTTA CGACTTGGTC TTGAACATTA CCAAAGAAGC TCGTTCCTTG 
    TTATCTGAGT ACAATATTTT GAAGAATGGT AAGGTTTTCG TTGAATCTAA CCACGAGGAA 
    TACTTCAAAA CTGCTGAAGA TCAGAAAGAT TCTATTGTCT CGTTGATCAA GGCCATCGAC 
    GAAGTCACTG TTGTTCGTGA TGCTTCCGAA ATTCCAGAAG GTTGCGTATT GCAATCTGTT 
    AACCCAGAAG TCAATGTACA TCTTCTCGTC AAGGGACACG TTGATATTGA TGCTGAAATT 
    GCGAAAGTTC AAAAGAAACT TGAAAAGGCT AAAAAATCCA AGAACGGTAT TGAACAAACC 
    ATTAACAGTA AGGATTACGA AACAAAGGCT AATACACAGG CCAAGGAAGC CAATAAAAGC 
    AAGCTGGATA ACACTGTTGC CGAAATCGAA GGTTTGGAAG CTACTATTGA AAACTTGAAG 
    CGTTTGAAAT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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