MSB2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MSB2
Entrez Gene ID	852897
Full Name	Msb2p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181143.3	NP_011528.3	Msb2p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The extracellular cysteine-rich domain of the transmembrane-anchor protein Opy2 binds to the Hkr1-Msb2 homology domain of the putative osmosensor Msb2 and the formation of the Opy2-Msb2 complex is essential for osmotic activation of Hog1.
Title: Binding of the Extracellular Eight-Cysteine Motif of Opy2 to the Putative Osmosensor Msb2 Is Essential for Activation of the Yeast High-Osmolarity Glycerol Pathway.

Our results confirm the existence of negative feedback systems targeting the protein levels of Ste11 and Msb2 and also hint at changes in the dissociation rates of intermediate signaling complexes
Title: Msb2 is a Ste11 membrane concentrator required for full activation of the HOG pathway.

interaction between Ste20 and Sho1 contributed to the activation of Hog1 by Hkr1, but not by Msb2.
Title: Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms.

The osmosensor Hkr1 is required for activation of the yeast cell wall integrity MAPK pathway signaling but Msb2 is not.
Title: Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2.

Data showed that Msb2p had a function in cell polarization through the adaptor protein Sho1p that Hkr1p did not.
Title: The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response.

The following MSB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02246	NM_001181143.3 Latest version!	Saccharomyces cerevisiae S288C Msb2p (MSB2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSB2 ( NM_001181143.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011528.3 Saccharomyces cerevisiae S288C Msb2p (MSB2), partial mRNA.

Price & Availability↑

CloneID	OSi02246
Clone ID Related Accession (Same CDS sequence)	NM_001181143.3
Accession Version	NM_001181143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Msb2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181143.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCAGTTTC CATTCGCTTG TCTCCTATCG ACCCTTGTAA TTAGTGGGTC ATTGGCCCGG 
GCCAGCCCCT TCGACTTTAT ATTCGGCAAT GGAACGCAAC AAGCTCAGAG CCAAAGCGAG 
AGTCAAGGTC AAGTTTCTTT CACCAATGAA GCTTCTCAGG ATAGTTCCAC CACCTCTTTG 
GTAACAGCCT ATTCTCAAGG TGTTCATTCG CACCAGTCTG CAACAATAGT GAGTGCCACA 
ATCTCTTCCC TCCCATCTAC TTGGTATGAT GCGAGCTCCA CTTCCCAGAC TTCTGTGTCA 
TATGCCAGTC AAGAATCCGA CTATGCCGTT AATCAAAACT CTTGGAGCGC GTCTACTAAT 
CAACTGCCAT CTACCAGTAC GACAAGCTAC TATGCGCCAA CCTTCAGTAC ATCGGCCGAT 
TTTGCTGCTT CTAGTGTAAA TGCAGCTTCT GATGTCTCCA CTGCCAGTGT TCCCATTGAT 
ACGAGTGCTA ATTCTATCCC TTTCACAACT ACAAGTAACA TAGAGACTAC AACGAGTGCA 
CCTCTCACTT CGGACACTCC ACTTATTTCC ACTAGTACGA TGTCCGCAGC TGATAACGTA 
TTTTCGTCAG CAAACCCTAT TTCTGCCTCC CTAACAACCA CCGATAGTTC AGAAAGTTTT 
GACCAAACTT CGACTGCTGG TGCCATTCCG GTGCAAAGTT CAGCAGATTT TAGTAGTTCT 
AGTGAAATTT TAGTACAAAG TTCGGCGGAT TTCAGTAGCC CTAGTTCTCC AACTACTACC 
GATATATCGC TATCAGCTGC CCCACTGCAA ACAAGTGAAT CAAGCAGTTT TACCACTGCA 
TCAGCAGCTC TACCAGTAAG TTCAACAGAC GTTGATGGCT CAAGCGCCTC ACCTGTAGTG 
AGCATGAGCG CCGCAGGACA AATAGCTAGC TCAAGCAGCA CAGATAATCC AACTATGTCA 
GAAACCTTTT CGTTAACATC TACAGAAGTT GATGGTTCCG ATGTTTCATC AACAGTGAGC 
GCATTATTAT CGGCTCCTTT TTTACAAACA AGTACTTCCA ACAGTTTCAG CATTGTTAGC 
CCATCGGTAT CTTTTGTTCC ATCACAGAGT TCCTCAGACG TTGCTAGCTC CAGTACTGCA 
AATGTAGTTA GTTCATCCTT TTCTGATATT CCACCGCAAA CTAGTACCTC AGGGAGCGTA 
GTTTCGGTAG CGCAATCCGC ATCTGCCCTC GCATTTCAAA GTTCAACAGA GGTATATGGT 
GCCAGTGCCT CGAGCACAAT GAGTTCATTA TTATCAACTA CTTCGCTACA GTCTACTACT 
TTGGATAGCT CAAGTTTAGC TAGCTCCTCT GCGTCGAGTT CAGACCTTAC AGATTATGGC 
GTCTCCAGTA CAGCAAGCAT ACCGCTGTTG TCAGCCTCAG AACAAGCAAG TACTTCCAGC 
AGTTTTAGCG TTGTTAGCCC TTCGGTATCT TTTGTTCCAT CACAAAGTTC CTCAGATGTT 
GCTAGCACCA GTGCTCCAAG TGTAGTTAGT TCATCCTTTT CTTATACTTC ACTGCAAGCA 
GGTGGCTCTA GCATGACCAA TCCCTCTTCA TCAACTATAG TATATTCAAG TAGTACTGGC 
AGTTCTGAGG AATCCGCTGC ATCTACAGCT TCTGCAACAC TGTCGGGCTC CTCGTCTACT 
TATATGGCAG GAAATTTGCA ATCACAGCCT CCATCCACTT CAAGTTTGCT TTCGGAGTCT 
CAAGCTACAA GCACTTCAGC TGTGCTAGCT AGCAGTTCTG TTTCTACAAC TTCACCCTAT 
ACCACTGCAG GTGGTGCATC TACAGAGGCC TCATCCCTCA TATCATCTAC ATCTGCGGAA 
ACTTCCCAGG TAAGTTATTC ACAAAGCACA ACTGCATTGC AAACTTCCTC ATTCGCATCG 
TCTTCAACAA CAGAAGGAAG TGAAACATCT AGTCAAGGTT TTTCTACCAG CTCTGTTTTA 
GTTCAAATGC CTTCTTCGAT TTCCAGCGAA TTCTCACCCT CTCAGACGAC AACTCAAATG 
AATTCTGCAA GCTCATCATC TCAGTACACT ATATCATCCA CTGGTATACT TTCTCAGGTT 
TCAGACACAT CGGTGTCTTA TACAACTTCA AGTTCGTCTG TTTCTCAAGT TTCAGACACA 
CCAGTTTCTT ATACAACTTC AAGTTCGTCT GTTTCTCAAG TTTCAGACAC ACCGGTTTCT 
TATACAACTT CAAGTTCGTC TGTTTCTCAA GTTTCAGACA CACCAGTTTC TTATACAACT 
TCAAGTTCAT CTGTTTCTCA AGTTTCAGAC ACACCGGTTT CTTATACAAC TTCAAGTTCG 
TCCGTTTCTC AAGTTTCAGA CACGTCAGTA CCTTCTACAA GTTCCAGATC GTCCGTTTCT 
CAAGTCTCAG ACACTCCGGT GCCTTCTACA AGTTCAAGGT CGTCCGTTTC TCAAACATCT 
AGCTCACTAC AGCCCACCAC TACATCCTCC CAACGTTTCA CCATTTCCAC TCATGGAGCG 
CTTTCTGAAA GTAGTTCTGT TAGCCAACAA GCTTCTGAGA TTACTAGCTC AATCAATGCA 
ACAGCTTCCG AATACCATAG CATCCAGACA ACCGCGGCTA CTCAATCCAC AACTCTATCT 
TTTACCGACG CAAACAGCAG TTCTGCTTCC GCTCCATTGG AAGTGGCAAC GTCTACGCCA 
ACCCCATCTT CAAAGGCATC CTCTCTGTTG CTTACACCAT CAACATCCTC TTTAAGTCAG 
GTTGCTACAA ATACTAATGT ACAGACGAGT TTAACAACGG AATCGACGAC CGTTTTAGAA 
CCATCAACGA CTAACAGTTC CAGTACGTTT AGTCTGGTCA CTTCAAGTGA CAACAATTGG 
TGGATTCCAA CTGAGTTAAT CACGCAGGCA CCAGAAGCTG CATCCACTGC ATCTTCTACC 
GTTGGAGGAA CACAAACTAT GACTTTGCCC CATGCAATTG CAGCCGCGAC ACAAGTTCCC 
GAGCCTGAGG GCTACACCCT AATCACAATA GGGTTCAAAA AAGCTTTGAA CTACGAATTT 
GTTGTATCAG AACCAAAATC ATCGGCTCAA ATCTTCGGAT ACTTGCCTGA AGCTCTGAAC 
ACACCTTTTA AGAACGTATT CACAAACATT ACGGTACTAC AAATAGTGCC ATTACAGGAT 
GACTCACTCA ACTACTTAGT AAGTGTTGCT GAAGTATACT TTCCAACTGC AGAAATAGAG 
GAGCTGTCAA ATCTAATTAC CAACTCTTCA AGCGCTTTTT ACACGGATGG AATGGGTACA 
GCAAAATCTA TGGCTGCAAT GGTTGATTCC TCAATACCGC TAACGGGCCT CTTACACGAT 
AGTAACAGCA ACTCTGGCGG ATCTTCGGAC GGATCCTCCT CCAGTAATTC GAACTCAGGA 
TCTTCAGGTT CAGGATCTAA TTCGAACTCC GGTGTGTCTT CATCTTCCGG AAATTCCTAT 
CAAGATGCCG GTACTTTGGA ATATTCATCC AAATCTAACT CCAACGTATC CACTTCTAGC 
AAATCAAAGA AAAAAATCAT TGGTTTAGTT ATCGGCGTTG TTGTTGGTGG ATGCTTATAT 
ATTTTATTCA TGATTTTTGC TTTCAAGTAT ATCATAAGAA GGCGGATTCA AAGTCAAGAA 
ATTATCAAGA ACCCAGAAAT TTCCAGTATC AGTTCAAGTG AATTTGGTGG AGAGAAAAAT 
TACAATAATG AAAAGAGAAT GAGCGTTCAA GAATCCATAA CACAATCTAT GCGAATTCAA 
AATTGGATGG ATGATAGTTA CTATGGTCAC GGGTTGACAA ATAATGACTC AACTCCAACC 
AGGCACAATA CATCGAGTTC CATACCAAAA ATTTCAAGAC CAATTGCTAG CCAAAACTCC 
CTGGGTTGGA ACGAAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011528.3
CDS	1..3921
Translation	MQFPFACLLSTLVISGSLARASPFDFIFGNGTQQAQSQSESQGQVSFTNEASQDSSTTSLVTAYSQGVHSHQSATIVSATISSLPSTWYDASSTSQTSVSYASQESDYAVNQNSWSASTNQLPSTSTTSYYAPTFSTSADFAASSVNAASDVSTASVPIDTSANSIPFTTTSNIETTTSAPLTSDTPLISTSTMSAADNVFSSANPISASLTTTDSSESFDQTSTAGAIPVQSSADFSSSSEILVQSSADFSSPSSPTTTDISLSAAPLQTSESSSFTTASAALPVSSTDVDGSSASPVVSMSAAGQIASSSSTDNPTMSETFSLTSTEVDGSDVSSTVSALLSAPFLQTSTSNSFSIVSPSVSFVPSQSSSDVASSSTANVVSSSFSDIPPQTSTSGSVVSVAQSASALAFQSSTEVYGASASSTMSSLLSTTSLQSTTLDSSSLASSSASSSDLTDYGVSSTASIPLLSASEQASTSSSFSVVSPSVSFVPSQSSSDVASTSAPSVVSSSFSYTSLQAGGSSMTNPSSSTIVYSSSTGSSEESAASTASATLSGSSSTYMAGNLQSQPPSTSSLLSESQATSTSAVLASSSVSTTSPYTTAGGASTEASSLISSTSAETSQVSYSQSTTALQTSSFASSSTTEGSETSSQGFSTSSVLVQMPSSISSEFSPSQTTTQMNSASSSSQYTISSTGILSQVSDTSVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTPVSYTTSSSSVSQVSDTSVPSTSSRSSVSQVSDTPVPSTSSRSSVSQTSSSLQPTTTSSQRFTISTHGALSESSSVSQQASEITSSINATASEYHSIQTTAATQSTTLSFTDANSSSASAPLEVATSTPTPSSKASSLLLTPSTSSLSQVATNTNVQTSLTTESTTVLEPSTTNSSSTFSLVTSSDNNWWIPTELITQAPEAASTASSTVGGTQTMTLPHAIAAATQVPEPEGYTLITIGFKKALNYEFVVSEPKSSAQIFGYLPEALNTPFKNVFTNITVLQIVPLQDDSLNYLVSVAEVYFPTAEIEELSNLITNSSSAFYTDGMGTAKSMAAMVDSSIPLTGLLHDSNSNSGGSSDGSSSSNSNSGSSGSGSNSNSGVSSSSGNSYQDAGTLEYSSKSNSNVSTSSKSKKKIIGLVIGVVVGGCLYILFMIFAFKYIIRRRIQSQEIIKNPEISSISSSEFGGEKNYNNEKRMSVQESITQSMRIQNWMDDSYYGHGLTNNDSTPTRHNTSSSIPKISRPIASQNSLGWNEV

Target ORF information:

RefSeq Version	NM_001181143.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Msb2p (MSB2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181143.3

ORF Insert Sequence:

ATGCAGTTTC CATTCGCTTG TCTCCTATCG ACCCTTGTAA TTAGTGGGTC ATTGGCCCGG 
GCCAGCCCCT TCGACTTTAT ATTCGGCAAT GGAACGCAAC AAGCTCAGAG CCAAAGCGAG 
AGTCAAGGTC AAGTTTCTTT CACCAATGAA GCTTCTCAGG ATAGTTCCAC CACCTCTTTG 
GTAACAGCCT ATTCTCAAGG TGTTCATTCG CACCAGTCTG CAACAATAGT GAGTGCCACA 
ATCTCTTCCC TCCCATCTAC TTGGTATGAT GCGAGCTCCA CTTCCCAGAC TTCTGTGTCA 
TATGCCAGTC AAGAATCCGA CTATGCCGTT AATCAAAACT CTTGGAGCGC GTCTACTAAT 
CAACTGCCAT CTACCAGTAC GACAAGCTAC TATGCGCCAA CCTTCAGTAC ATCGGCCGAT 
TTTGCTGCTT CTAGTGTAAA TGCAGCTTCT GATGTCTCCA CTGCCAGTGT TCCCATTGAT 
ACGAGTGCTA ATTCTATCCC TTTCACAACT ACAAGTAACA TAGAGACTAC AACGAGTGCA 
CCTCTCACTT CGGACACTCC ACTTATTTCC ACTAGTACGA TGTCCGCAGC TGATAACGTA 
TTTTCGTCAG CAAACCCTAT TTCTGCCTCC CTAACAACCA CCGATAGTTC AGAAAGTTTT 
GACCAAACTT CGACTGCTGG TGCCATTCCG GTGCAAAGTT CAGCAGATTT TAGTAGTTCT 
AGTGAAATTT TAGTACAAAG TTCGGCGGAT TTCAGTAGCC CTAGTTCTCC AACTACTACC 
GATATATCGC TATCAGCTGC CCCACTGCAA ACAAGTGAAT CAAGCAGTTT TACCACTGCA 
TCAGCAGCTC TACCAGTAAG TTCAACAGAC GTTGATGGCT CAAGCGCCTC ACCTGTAGTG 
AGCATGAGCG CCGCAGGACA AATAGCTAGC TCAAGCAGCA CAGATAATCC AACTATGTCA 
GAAACCTTTT CGTTAACATC TACAGAAGTT GATGGTTCCG ATGTTTCATC AACAGTGAGC 
GCATTATTAT CGGCTCCTTT TTTACAAACA AGTACTTCCA ACAGTTTCAG CATTGTTAGC 
CCATCGGTAT CTTTTGTTCC ATCACAGAGT TCCTCAGACG TTGCTAGCTC CAGTACTGCA 
AATGTAGTTA GTTCATCCTT TTCTGATATT CCACCGCAAA CTAGTACCTC AGGGAGCGTA 
GTTTCGGTAG CGCAATCCGC ATCTGCCCTC GCATTTCAAA GTTCAACAGA GGTATATGGT 
GCCAGTGCCT CGAGCACAAT GAGTTCATTA TTATCAACTA CTTCGCTACA GTCTACTACT 
TTGGATAGCT CAAGTTTAGC TAGCTCCTCT GCGTCGAGTT CAGACCTTAC AGATTATGGC 
GTCTCCAGTA CAGCAAGCAT ACCGCTGTTG TCAGCCTCAG AACAAGCAAG TACTTCCAGC 
AGTTTTAGCG TTGTTAGCCC TTCGGTATCT TTTGTTCCAT CACAAAGTTC CTCAGATGTT 
GCTAGCACCA GTGCTCCAAG TGTAGTTAGT TCATCCTTTT CTTATACTTC ACTGCAAGCA 
GGTGGCTCTA GCATGACCAA TCCCTCTTCA TCAACTATAG TATATTCAAG TAGTACTGGC 
AGTTCTGAGG AATCCGCTGC ATCTACAGCT TCTGCAACAC TGTCGGGCTC CTCGTCTACT 
TATATGGCAG GAAATTTGCA ATCACAGCCT CCATCCACTT CAAGTTTGCT TTCGGAGTCT 
CAAGCTACAA GCACTTCAGC TGTGCTAGCT AGCAGTTCTG TTTCTACAAC TTCACCCTAT 
ACCACTGCAG GTGGTGCATC TACAGAGGCC TCATCCCTCA TATCATCTAC ATCTGCGGAA 
ACTTCCCAGG TAAGTTATTC ACAAAGCACA ACTGCATTGC AAACTTCCTC ATTCGCATCG 
TCTTCAACAA CAGAAGGAAG TGAAACATCT AGTCAAGGTT TTTCTACCAG CTCTGTTTTA 
GTTCAAATGC CTTCTTCGAT TTCCAGCGAA TTCTCACCCT CTCAGACGAC AACTCAAATG 
AATTCTGCAA GCTCATCATC TCAGTACACT ATATCATCCA CTGGTATACT TTCTCAGGTT 
TCAGACACAT CGGTGTCTTA TACAACTTCA AGTTCGTCTG TTTCTCAAGT TTCAGACACA 
CCAGTTTCTT ATACAACTTC AAGTTCGTCT GTTTCTCAAG TTTCAGACAC ACCGGTTTCT 
TATACAACTT CAAGTTCGTC TGTTTCTCAA GTTTCAGACA CACCAGTTTC TTATACAACT 
TCAAGTTCAT CTGTTTCTCA AGTTTCAGAC ACACCGGTTT CTTATACAAC TTCAAGTTCG 
TCCGTTTCTC AAGTTTCAGA CACGTCAGTA CCTTCTACAA GTTCCAGATC GTCCGTTTCT 
CAAGTCTCAG ACACTCCGGT GCCTTCTACA AGTTCAAGGT CGTCCGTTTC TCAAACATCT 
AGCTCACTAC AGCCCACCAC TACATCCTCC CAACGTTTCA CCATTTCCAC TCATGGAGCG 
CTTTCTGAAA GTAGTTCTGT TAGCCAACAA GCTTCTGAGA TTACTAGCTC AATCAATGCA 
ACAGCTTCCG AATACCATAG CATCCAGACA ACCGCGGCTA CTCAATCCAC AACTCTATCT 
TTTACCGACG CAAACAGCAG TTCTGCTTCC GCTCCATTGG AAGTGGCAAC GTCTACGCCA 
ACCCCATCTT CAAAGGCATC CTCTCTGTTG CTTACACCAT CAACATCCTC TTTAAGTCAG 
GTTGCTACAA ATACTAATGT ACAGACGAGT TTAACAACGG AATCGACGAC CGTTTTAGAA 
CCATCAACGA CTAACAGTTC CAGTACGTTT AGTCTGGTCA CTTCAAGTGA CAACAATTGG 
TGGATTCCAA CTGAGTTAAT CACGCAGGCA CCAGAAGCTG CATCCACTGC ATCTTCTACC 
GTTGGAGGAA CACAAACTAT GACTTTGCCC CATGCAATTG CAGCCGCGAC ACAAGTTCCC 
GAGCCTGAGG GCTACACCCT AATCACAATA GGGTTCAAAA AAGCTTTGAA CTACGAATTT 
GTTGTATCAG AACCAAAATC ATCGGCTCAA ATCTTCGGAT ACTTGCCTGA AGCTCTGAAC 
ACACCTTTTA AGAACGTATT CACAAACATT ACGGTACTAC AAATAGTGCC ATTACAGGAT 
GACTCACTCA ACTACTTAGT AAGTGTTGCT GAAGTATACT TTCCAACTGC AGAAATAGAG 
GAGCTGTCAA ATCTAATTAC CAACTCTTCA AGCGCTTTTT ACACGGATGG AATGGGTACA 
GCAAAATCTA TGGCTGCAAT GGTTGATTCC TCAATACCGC TAACGGGCCT CTTACACGAT 
AGTAACAGCA ACTCTGGCGG ATCTTCGGAC GGATCCTCCT CCAGTAATTC GAACTCAGGA 
TCTTCAGGTT CAGGATCTAA TTCGAACTCC GGTGTGTCTT CATCTTCCGG AAATTCCTAT 
CAAGATGCCG GTACTTTGGA ATATTCATCC AAATCTAACT CCAACGTATC CACTTCTAGC 
AAATCAAAGA AAAAAATCAT TGGTTTAGTT ATCGGCGTTG TTGTTGGTGG ATGCTTATAT 
ATTTTATTCA TGATTTTTGC TTTCAAGTAT ATCATAAGAA GGCGGATTCA AAGTCAAGAA 
ATTATCAAGA ACCCAGAAAT TTCCAGTATC AGTTCAAGTG AATTTGGTGG AGAGAAAAAT 
TACAATAATG AAAAGAGAAT GAGCGTTCAA GAATCCATAA CACAATCTAT GCGAATTCAA 
AATTGGATGG ATGATAGTTA CTATGGTCAC GGGTTGACAA ATAATGACTC AACTCCAACC 
AGGCACAATA CATCGAGTTC CATACCAAAA ATTTCAAGAC CAATTGCTAG CCAAAACTCC 
CTGGGTTGGA ACGAAGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MSB2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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