ORF » Species Summary » Saccharomyces cerevisiae S288C » LEU1 cDNA ORF clone

LEU1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following LEU1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEU1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02226 NM_001180874.1
Latest version!		 Saccharomyces cerevisiae S288C 3-isopropylmalate dehydratase LEU1 (LEU1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LEU1 ( NM_001180874.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011506.1 Saccharomyces cerevisiae S288C 3-isopropylmalate dehydratase LEU1 (LEU1), partial mRNA.
    Price & Availability↑
    CloneID OSi02226
    Clone ID Related Accession (Same CDS sequence) NM_001180874.1
    Accession Version NM_001180874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product 3-isopropylmalate dehydratase LEU1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGTTTACA CTCCATCCAA GGGTCCAAGA ACTCTTTACG ATAAGGTTTT TGATGCACAT 
    GTTGTCCATC AAGATGAAAA TGGTTCCTTT TTGTTGTATA TCGACAGACA CTTGGTTCAT 
    GAAGTCACCT CTCCACAAGC TTTCGAAGGC CTAGAAAATG CCGGCAGAAA GGTCAGAAGA 
    GTTGATTGTA CTCTAGCTAC TGTAGATCAT AATATTCCAA CTGAATCAAG AAAGAATTTC 
    AAATCACTAG ACACATTTAT CAAGCAAACT GATTCGCGTT TACAAGTCAA AACTTTAGAG 
    AATAATGTTA AACAGTTCGG CGTTCCATAT TTCGGTATGA GTGATGCCAG ACAAGGTATC 
    GTCCACACCA TCGGTCCCGA AGAAGGTTTC ACTTTACCAG GTACCACTGT TGTTTGTGGT 
    GACTCTCATA CCTCTACTCA CGGTGCCTTT GGTTCGCTGG CCTTTGGTAT TGGTACTTCT 
    GAAGTTGAAC ACGTCTTGGC CACTCAAACA ATTATCCAAG CTAAATCGAA AAACATGAGA 
    ATTACTGTTA ATGGTAAGCT ATCACCTGGT ATCACATCTA AAGATTTGAT TTTGTATATT 
    ATTGGTCTGA TAGGTACTGC AGGTGGTACT GGTTGCGTTA TTGAATTCGC AGGTGAAGCC 
    ATTGAAGCTC TTTCTATGGA AGCACGTATG TCCATGTGTA ACATGGCCAT CGAAGCTGGT 
    GCAAGAGCAG GTATGATTAA ACCAGACGAA ACTACTTTCC AATACACCAA AGGCAGACCT 
    TTGGCTCCAA AAGGTGCCGA ATGGGAAAAG GCTGTTGCCT ACTGGAAAAC TTTGAAAACA 
    GACGAAGGTG CCAAATTTGA CCACGAAATC AACATTGAAG CTGTCGACGT GATTCCAACT 
    ATTACATGGG GTACTTCTCC TCAAGATGCC TTACCAATTA CTGGTTCCGT ACCAGATCCA 
    AAAAATGTTA CAGACCCAAT AAAGAAATCT GGTATGGAAA GAGCCTTAGC CTATATGGGT 
    TTGGAACCTA ACACACCGCT AAAGAGCATC AAAGTAGATA AAGTCTTTAT TGGATCTTGT 
    ACCAATGGCC GTATTGAAGA TTTAAGAAGT GCTGCAGCTG TAGTTAGGGG TCAAAAATTG 
    GCTAGTAACA TCAAATTAGC TATGGTTGTT CCGGGATCTG GTTTGGTCAA AAAACAAGCC 
    GAAGCAGAAG GTTTGGACAA GATTTTCCAA GAAGCTGGTT TTGAATGGAG AGAAGCTGGT 
    TGTTCAATAT GTTTAGGTAT GAACCCTGAT ATTTTGGATG CCTATGAACG TTGTGCTTCT 
    ACTTCCAACA GAAATTTCGA AGGTCGTCAA GGTGCCTTGT CTCGTACACA TCTAATGTCC 
    CCAGCCATGG CGGCTGCTGC AGGTATCGCA GGTCACTTCG TAGATATTAG AGAATTCGAA 
    TATAAGGATC AAGACCAAAG TAGTCCAAAG GTTGAGGTCA CTTCCGAAGA TGAAAAAGAG 
    CTTGAAAGTG CTGCTTACGA TCACGCTGAA CCTGTCCAAC CCGAAGACGC TCCCCAAGAT 
    ATTGCCAATG ATGAATTAAA AGATATTCCA GTGAAGTCAG ATGATACACC TGCTAAACCT 
    AGCTCTTCCG GCATGAAGCC ATTTTTGACT TTGGAAGGTA TTAGCGCACC ATTAGATAAG 
    GCCAATGTTG ATACAGACGC TATTATCCCA AAGCAATTTT TAAAGACAAT TAAGAGAACG 
    GGTTTGAAAA AAGGGTTGTT TTATGAATGG CGTTTCCGTA AAGACGATCA AGGTAAGGAT 
    CAAGAAACCG ATTTTGTATT AAATGTTGAA CCTTGGAGGG AAGCTGAAAT ATTGGTTGTT 
    ACTGGTGACA ATTTCGGATG TGGTTCTTCC AGAGAACACG CACCATGGGC TCTAAAAGAC 
    TTTGGTATAA AATCCATCAT TGCACCTTCT TACGGTGATA TTTTCTACAA CAACTCTTTC 
    AAGAACGGCT TATTACCTAT TAGATTGGAT CAACAAATAA TCATTGACAA ATTGATCCCT 
    ATTGCTAATA AGGGTGGTAA GCTCTGCGTC GATTTACCAA ATCAAAAAAT TTTGGATTCC 
    GATGGCAATG TGCTCGTTGA TCATTTTGAA ATAGAACCTT TCAGAAAGCA CTGCCTAGTT 
    AACGGTCTTG ATGATATTGG TATTACATTG CAAAAGGAAG AATACATTTC CAGATACGAA 
    GCTTTGAGAA GAGAGAAATA CTCATTCTTG GAAGGTGGAT CAAAATTATT AAAATTTGAC 
    AACGTTCCAA AAAGAAAAGC TGTTACAACT ACTTTCGATA AAGTCCACCA GGATTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011506.1
    CDS1..2340
    Translation

    Target ORF information:

    RefSeq Version NM_001180874.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C 3-isopropylmalate dehydratase LEU1 (LEU1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001180874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGTTTACA CTCCATCCAA GGGTCCAAGA ACTCTTTACG ATAAGGTTTT TGATGCACAT 
    GTTGTCCATC AAGATGAAAA TGGTTCCTTT TTGTTGTATA TCGACAGACA CTTGGTTCAT 
    GAAGTCACCT CTCCACAAGC TTTCGAAGGC CTAGAAAATG CCGGCAGAAA GGTCAGAAGA 
    GTTGATTGTA CTCTAGCTAC TGTAGATCAT AATATTCCAA CTGAATCAAG AAAGAATTTC 
    AAATCACTAG ACACATTTAT CAAGCAAACT GATTCGCGTT TACAAGTCAA AACTTTAGAG 
    AATAATGTTA AACAGTTCGG CGTTCCATAT TTCGGTATGA GTGATGCCAG ACAAGGTATC 
    GTCCACACCA TCGGTCCCGA AGAAGGTTTC ACTTTACCAG GTACCACTGT TGTTTGTGGT 
    GACTCTCATA CCTCTACTCA CGGTGCCTTT GGTTCGCTGG CCTTTGGTAT TGGTACTTCT 
    GAAGTTGAAC ACGTCTTGGC CACTCAAACA ATTATCCAAG CTAAATCGAA AAACATGAGA 
    ATTACTGTTA ATGGTAAGCT ATCACCTGGT ATCACATCTA AAGATTTGAT TTTGTATATT 
    ATTGGTCTGA TAGGTACTGC AGGTGGTACT GGTTGCGTTA TTGAATTCGC AGGTGAAGCC 
    ATTGAAGCTC TTTCTATGGA AGCACGTATG TCCATGTGTA ACATGGCCAT CGAAGCTGGT 
    GCAAGAGCAG GTATGATTAA ACCAGACGAA ACTACTTTCC AATACACCAA AGGCAGACCT 
    TTGGCTCCAA AAGGTGCCGA ATGGGAAAAG GCTGTTGCCT ACTGGAAAAC TTTGAAAACA 
    GACGAAGGTG CCAAATTTGA CCACGAAATC AACATTGAAG CTGTCGACGT GATTCCAACT 
    ATTACATGGG GTACTTCTCC TCAAGATGCC TTACCAATTA CTGGTTCCGT ACCAGATCCA 
    AAAAATGTTA CAGACCCAAT AAAGAAATCT GGTATGGAAA GAGCCTTAGC CTATATGGGT 
    TTGGAACCTA ACACACCGCT AAAGAGCATC AAAGTAGATA AAGTCTTTAT TGGATCTTGT 
    ACCAATGGCC GTATTGAAGA TTTAAGAAGT GCTGCAGCTG TAGTTAGGGG TCAAAAATTG 
    GCTAGTAACA TCAAATTAGC TATGGTTGTT CCGGGATCTG GTTTGGTCAA AAAACAAGCC 
    GAAGCAGAAG GTTTGGACAA GATTTTCCAA GAAGCTGGTT TTGAATGGAG AGAAGCTGGT 
    TGTTCAATAT GTTTAGGTAT GAACCCTGAT ATTTTGGATG CCTATGAACG TTGTGCTTCT 
    ACTTCCAACA GAAATTTCGA AGGTCGTCAA GGTGCCTTGT CTCGTACACA TCTAATGTCC 
    CCAGCCATGG CGGCTGCTGC AGGTATCGCA GGTCACTTCG TAGATATTAG AGAATTCGAA 
    TATAAGGATC AAGACCAAAG TAGTCCAAAG GTTGAGGTCA CTTCCGAAGA TGAAAAAGAG 
    CTTGAAAGTG CTGCTTACGA TCACGCTGAA CCTGTCCAAC CCGAAGACGC TCCCCAAGAT 
    ATTGCCAATG ATGAATTAAA AGATATTCCA GTGAAGTCAG ATGATACACC TGCTAAACCT 
    AGCTCTTCCG GCATGAAGCC ATTTTTGACT TTGGAAGGTA TTAGCGCACC ATTAGATAAG 
    GCCAATGTTG ATACAGACGC TATTATCCCA AAGCAATTTT TAAAGACAAT TAAGAGAACG 
    GGTTTGAAAA AAGGGTTGTT TTATGAATGG CGTTTCCGTA AAGACGATCA AGGTAAGGAT 
    CAAGAAACCG ATTTTGTATT AAATGTTGAA CCTTGGAGGG AAGCTGAAAT ATTGGTTGTT 
    ACTGGTGACA ATTTCGGATG TGGTTCTTCC AGAGAACACG CACCATGGGC TCTAAAAGAC 
    TTTGGTATAA AATCCATCAT TGCACCTTCT TACGGTGATA TTTTCTACAA CAACTCTTTC 
    AAGAACGGCT TATTACCTAT TAGATTGGAT CAACAAATAA TCATTGACAA ATTGATCCCT 
    ATTGCTAATA AGGGTGGTAA GCTCTGCGTC GATTTACCAA ATCAAAAAAT TTTGGATTCC 
    GATGGCAATG TGCTCGTTGA TCATTTTGAA ATAGAACCTT TCAGAAAGCA CTGCCTAGTT 
    AACGGTCTTG ATGATATTGG TATTACATTG CAAAAGGAAG AATACATTTC CAGATACGAA 
    GCTTTGAGAA GAGAGAAATA CTCATTCTTG GAAGGTGGAT CAAAATTATT AAAATTTGAC 
    AACGTTCCAA AAAGAAAAGC TGTTACAACT ACTTTCGATA AAGTCCACCA GGATTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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