ALK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ALK1
Entrez Gene ID	852863
Full Name	protein kinase ALK1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180886.1	NP_011494.1	protein kinase ALK1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C11583g	XP_452717.1
Eremothecium gossypii	AGOS_AFR492W	NP_986039.1
Saccharomyces cerevisiae (baker's yeast)	ALK2	NP_009544.1
Saccharomyces cerevisiae (baker's yeast)	ALK1	NP_011494.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that deletion of ALK1 and ALK2 haspin paralogs causes the mislocalization of polarisome components.
Title: Yeast haspin kinase regulates polarity cues necessary for mitotic spindle positioning and is required to tolerate mitotic arrest.

Overexpression of ALK2, but not of ALK1, causes a mitotic arrest, which is correlated to the kinase activity of the protein
Title: Alk1 and Alk2 are two new cell cycle-regulated haspin-like proteins in budding yeast.

The following ALK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02214	NM_001180886.1 Latest version!	Saccharomyces cerevisiae S288C protein kinase ALK1 (ALK1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALK1 ( NM_001180886.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011494.1 Saccharomyces cerevisiae S288C protein kinase ALK1 (ALK1), partial mRNA.

Price & Availability↑

CloneID	OSi02214
Clone ID Related Accession (Same CDS sequence)	NM_001180886.1
Accession Version	NM_001180886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase ALK1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTACTTC ATTTTGACAT TGTAATTCAA TTACTATCAA GTCATACCCT TAAATCGCAC 
CAAGTAGAGC CCCCCATGGA TTTTGAAACG TCGTTCGAAG AATTTGTCGA AGATAAACGA 
TTCATTGCTC TAGAGGTATC TGATAATGAT GACGATTGTG ACACTGATTT GACTGCTGAC 
ACAGCTGACG AGCTGGAAAG CTCTGCTATC TTAAAGATGA GGGAGTCTGA TGCATCTTTA 
AATGTTACCA CCGGCAATAA TACATCAAGG AAAACGACTT CCAATTCCAA AAAACGATGG 
TCTCTATTAT CCAATCATTC TGCTGTTTCA TCATCTAAAT CTAAGAAAAG ATGGTCTGTA 
CTTTCTTCAA GCTTTACCAG TGAATCTCAT AAGGACAGGG AATCGAGGAA CGTTTTGCAA 
CAAAAAAGAA AATCCTTGCA GAGTTATTCA AGCTTAGATA CGGTAGCATC TAATAGCTCT 
ATCTCTGCTT CTTCTTCTTT GAAAAGATCG TCCACTGGTC TTTCCCTCAG ACAATTGTTT 
ACCAAGATAG GCATAAACGA TGATATATCA CAACCTGGGA TCGGGATTCC GCAAGGTAAG 
GAAAATCTGC CTCCAACTAT GGGCAAAAAA AATTCATCAA TTGCTTCCAC TAGTTCGGAA 
AACAGACTTA GGACACCATT GAAGCCGCTT GTTAATCATT CTAAAAGGCC CACTTCACAA 
CCACAACAGC AACAACCACT ATATAATGCA TCATTGTCCT CGAGAAGATC GTCGATTTCT 
TCCACTGTCT CTTCTTCCAG CTCGTCTAAA TGGAGATTCT GGAAGAGAAA TAAGAACCAG 
ACACCTGCAT TGTTGCAACC AGACCACCAT TCTTTAAAAA CCTTTCCTGC AGTAAACCGT 
CGTGATTCCA TGACGCCCGT TGAACCAAGA AATATGGTAA AACATAAGAC ATCCTTTTCA 
GATTTCCATA AGACTATCTT CAGTTCAAAT ACGTATTCTG AAAGCAGCGA TACAATATCT 
TCCATGGAAA TTACTTTGAA AAACAAAGCT TCTTCCTCAA GTCTATCTTT GAACGTGTTG 
AAGAAAAGGA ATTCGCAATC AAGTTTGAAG CACAAAAGTT CACATGCATC CTTACAAAAA 
TTCAAAAGAA ACAAAGGAAA GTCCTCCATG ATAGCACCAT CGACAGCAAC TAATAGCAGC 
AATGATGACT CTTGTAGCTA CAGTTCCAAA AATTCTACGT TGAGTCACCG AATATCGTTA 
CCTGTACCGG ACCAAGTTTC CCGTGATAAA ATTCAGAACA AATTAAGATA TTCGACCTCA 
TTACTCTCAT TAAATTCTAA ATCATCTTTA CCGATGAACA AAAATGATCA TGATGAGACA 
CTTCTAAGAC AGATTTTACT AAATTGTGAT ATAAAGAGAA TTTTAAACCC CGCAAAAGGA 
GATGTATTGC CTTTAATCAA CGATGTAAAC CATTTGTCAT CAATACAATT AACATCAAAT 
GTTTGGCAAA TTGGTGAAGT AATCTGTAAA AAAGTTTCTC TCGGTACTAT TGATGACATT 
ACATGGGATA GAAAGTTCTT ATCTTTGCAG GAACTGGAAA AACTCAAAAT CATGCAACAA 
AAATTTGATG GCATACCGCA ACTATTGAAA AGTTTTGTTG TTAAAGAGGC AAATGGGGGG 
TTGTATTTGT ACTTACTATT CAAAGATCAC GGTACCCCAA TTTCGTTAAT CTCACTGAAA 
AACTGGAAGC AGATTCTAAA GATTTTTTGG TCTTGTGCTG GTATAATTCA TGGGTTGGAA 
AAAAATTTGA AATTTGAACA TCGTAATCTT ACTTTGGATA ATATTTTGAT AGATGGTAAT 
GGGAATATAA CAATTATTGA TTTTAAATGC AGTAGATTGC AAACTCCTCA AGATGATGTT 
TTATATCTAC GGCTAGATCA TCCACTCTTT TTTCTAAATG GGAAAGACAA GAGTAAAATA 
AACGAATACC AGTATCAATT TGAGTTTGAA ATCTATCAAA GTATGCGGAT CTTGTTGAAC 
ATGGACGCGT CAGCTTTTGA GCCCATGACA AATTTGTATT GGTTATATTA CCTGTCACGC 
GTTTTACTAA AGTTTGGTGA CAGGAAGCTT GGTAAGAATG ATGCAAACAG GGATAAAATG 
GCAAGGGTGA TAAACCATTT GGAAATGAAC TTGGCAGTTC ATAAAAGAGG GGGGCAGTTA 
TTCAAAAGAT TGGAGACGGA GGACATTAAG AATACTGGAG ATTTACTAAA ACTTTATAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011494.1
CDS	1..2283
Translation	MLLHFDIVIQLLSSHTLKSHQVEPPMDFETSFEEFVEDKRFIALEVSDNDDDCDTDLTADTADELESSAILKMRESDASLNVTTGNNTSRKTTSNSKKRWSLLSNHSAVSSSKSKKRWSVLSSSFTSESHKDRESRNVLQQKRKSLQSYSSLDTVASNSSISASSSLKRSSTGLSLRQLFTKIGINDDISQPGIGIPQGKENLPPTMGKKNSSIASTSSENRLRTPLKPLVNHSKRPTSQPQQQQPLYNASLSSRRSSISSTVSSSSSSKWRFWKRNKNQTPALLQPDHHSLKTFPAVNRRDSMTPVEPRNMVKHKTSFSDFHKTIFSSNTYSESSDTISSMEITLKNKASSSSLSLNVLKKRNSQSSLKHKSSHASLQKFKRNKGKSSMIAPSTATNSSNDDSCSYSSKNSTLSHRISLPVPDQVSRDKIQNKLRYSTSLLSLNSKSSLPMNKNDHDETLLRQILLNCDIKRILNPAKGDVLPLINDVNHLSSIQLTSNVWQIGEVICKKVSLGTIDDITWDRKFLSLQELEKLKIMQQKFDGIPQLLKSFVVKEANGGLYLYLLFKDHGTPISLISLKNWKQILKIFWSCAGIIHGLEKNLKFEHRNLTLDNILIDGNGNITIIDFKCSRLQTPQDDVLYLRLDHPLFFLNGKDKSKINEYQYQFEFEIYQSMRILLNMDASAFEPMTNLYWLYYLSRVLLKFGDRKLGKNDANRDKMARVINHLEMNLAVHKRGGQLFKRLETEDIKNTGDLLKLYK

Target ORF information:

RefSeq Version	NM_001180886.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase ALK1 (ALK1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180886.1

ORF Insert Sequence:

ATGCTACTTC ATTTTGACAT TGTAATTCAA TTACTATCAA GTCATACCCT TAAATCGCAC 
CAAGTAGAGC CCCCCATGGA TTTTGAAACG TCGTTCGAAG AATTTGTCGA AGATAAACGA 
TTCATTGCTC TAGAGGTATC TGATAATGAT GACGATTGTG ACACTGATTT GACTGCTGAC 
ACAGCTGACG AGCTGGAAAG CTCTGCTATC TTAAAGATGA GGGAGTCTGA TGCATCTTTA 
AATGTTACCA CCGGCAATAA TACATCAAGG AAAACGACTT CCAATTCCAA AAAACGATGG 
TCTCTATTAT CCAATCATTC TGCTGTTTCA TCATCTAAAT CTAAGAAAAG ATGGTCTGTA 
CTTTCTTCAA GCTTTACCAG TGAATCTCAT AAGGACAGGG AATCGAGGAA CGTTTTGCAA 
CAAAAAAGAA AATCCTTGCA GAGTTATTCA AGCTTAGATA CGGTAGCATC TAATAGCTCT 
ATCTCTGCTT CTTCTTCTTT GAAAAGATCG TCCACTGGTC TTTCCCTCAG ACAATTGTTT 
ACCAAGATAG GCATAAACGA TGATATATCA CAACCTGGGA TCGGGATTCC GCAAGGTAAG 
GAAAATCTGC CTCCAACTAT GGGCAAAAAA AATTCATCAA TTGCTTCCAC TAGTTCGGAA 
AACAGACTTA GGACACCATT GAAGCCGCTT GTTAATCATT CTAAAAGGCC CACTTCACAA 
CCACAACAGC AACAACCACT ATATAATGCA TCATTGTCCT CGAGAAGATC GTCGATTTCT 
TCCACTGTCT CTTCTTCCAG CTCGTCTAAA TGGAGATTCT GGAAGAGAAA TAAGAACCAG 
ACACCTGCAT TGTTGCAACC AGACCACCAT TCTTTAAAAA CCTTTCCTGC AGTAAACCGT 
CGTGATTCCA TGACGCCCGT TGAACCAAGA AATATGGTAA AACATAAGAC ATCCTTTTCA 
GATTTCCATA AGACTATCTT CAGTTCAAAT ACGTATTCTG AAAGCAGCGA TACAATATCT 
TCCATGGAAA TTACTTTGAA AAACAAAGCT TCTTCCTCAA GTCTATCTTT GAACGTGTTG 
AAGAAAAGGA ATTCGCAATC AAGTTTGAAG CACAAAAGTT CACATGCATC CTTACAAAAA 
TTCAAAAGAA ACAAAGGAAA GTCCTCCATG ATAGCACCAT CGACAGCAAC TAATAGCAGC 
AATGATGACT CTTGTAGCTA CAGTTCCAAA AATTCTACGT TGAGTCACCG AATATCGTTA 
CCTGTACCGG ACCAAGTTTC CCGTGATAAA ATTCAGAACA AATTAAGATA TTCGACCTCA 
TTACTCTCAT TAAATTCTAA ATCATCTTTA CCGATGAACA AAAATGATCA TGATGAGACA 
CTTCTAAGAC AGATTTTACT AAATTGTGAT ATAAAGAGAA TTTTAAACCC CGCAAAAGGA 
GATGTATTGC CTTTAATCAA CGATGTAAAC CATTTGTCAT CAATACAATT AACATCAAAT 
GTTTGGCAAA TTGGTGAAGT AATCTGTAAA AAAGTTTCTC TCGGTACTAT TGATGACATT 
ACATGGGATA GAAAGTTCTT ATCTTTGCAG GAACTGGAAA AACTCAAAAT CATGCAACAA 
AAATTTGATG GCATACCGCA ACTATTGAAA AGTTTTGTTG TTAAAGAGGC AAATGGGGGG 
TTGTATTTGT ACTTACTATT CAAAGATCAC GGTACCCCAA TTTCGTTAAT CTCACTGAAA 
AACTGGAAGC AGATTCTAAA GATTTTTTGG TCTTGTGCTG GTATAATTCA TGGGTTGGAA 
AAAAATTTGA AATTTGAACA TCGTAATCTT ACTTTGGATA ATATTTTGAT AGATGGTAAT 
GGGAATATAA CAATTATTGA TTTTAAATGC AGTAGATTGC AAACTCCTCA AGATGATGTT 
TTATATCTAC GGCTAGATCA TCCACTCTTT TTTCTAAATG GGAAAGACAA GAGTAAAATA 
AACGAATACC AGTATCAATT TGAGTTTGAA ATCTATCAAA GTATGCGGAT CTTGTTGAAC 
ATGGACGCGT CAGCTTTTGA GCCCATGACA AATTTGTATT GGTTATATTA CCTGTCACGC 
GTTTTACTAA AGTTTGGTGA CAGGAAGCTT GGTAAGAATG ATGCAAACAG GGATAAAATG 
GCAAGGGTGA TAAACCATTT GGAAATGAAC TTGGCAGTTC ATAAAAGAGG GGGGCAGTTA 
TTCAAAAGAT TGGAGACGGA GGACATTAAG AATACTGGAG ATTTACTAAA ACTTTATAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ALK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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