TIF4632 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TIF4632
Entrez Gene ID	852833
Full Name	translation initiation factor eIF4G
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180914.1	NP_011466.1	translation initiation factor eIF4G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results reveal and characterize an unexpected interdependence between the two RNA helicases and eIF4G, and suggest that Ded1p is an integral part of eIF4F, the complex comprising eIF4G, eIF4A, and eIF4E.
Title: Coupling between the DEAD-box RNA helicases Ded1p and eIF4A.

Data show that that deletion of eIF4G1, but not eIF4G2, impairs growth and global translation initiation rates.
Title: Functional overlap between eIF4G isoforms in Saccharomyces cerevisiae.

involvement of yeast eIF4G in pre-mRNA metabolism; Tif4631p and Tif4632p interact efficiently with U1 snRNA; Tif4631p and Tif4632p associate with protein components of the splicing machinery, namely Snu71p and Prp11p.
Title: Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events.

structure of the complex formed by yeast eIF4G's middle domain and full-length eIF4A at 2.6-A resolution
Title: Crystal structure of the yeast eIF4A-eIF4G complex: an RNA-helicase controlled by protein-protein interactions.

The following TIF4632 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIF4632 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02191	NM_001180914.1 Latest version!	Saccharomyces cerevisiae S288C translation initiation factor eIF4G (TIF4632), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIF4632 ( NM_001180914.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011466.1 Saccharomyces cerevisiae S288C translation initiation factor eIF4G (TIF4632), partial mRNA.

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CloneID	OSi02191
Clone ID Related Accession (Same CDS sequence)	NM_001180914.1
Accession Version	NM_001180914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	translation initiation factor eIF4G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTGACC AAAGAGGTCC ACCGCCCCCA CACCCGCAGC AAGCCAATGG CTACAAGAAA 
TTTCCTCCTC ATGATAACCA ATACTCTGGA GCCAATAATA GTCAGCCAAA TAACCACTAC 
AATGAAAATC TTTACAGTGC AAGGGAACCT CACAATAACA AGCAATACCA GTCGAAAAAT 
GGCAAATACG GGACAAATAA ATACAATAAT CGTAATAATA GTCAAGGAAA TGCACAGTAC 
TACAATAACA GATTCAATAA TGGCTACAGA CTGAATAACA ACGACTATAA CCCGGCAATG 
TTGCCAGGTA TGCAATGGCC AGCTAACTAC TATGCTCCTC AGATGTATTA TATACCCCAA 
CAAATGGTTC CAGTTGCTTC TCCACCATAT ACACACCAAC CACTCAACAC TAATCCCGAA 
CCTCCCTCTA CACCTAAAAC TACAAAAATA GAAATCACTA CGAAAACTGG TGAACGTTTA 
AATTTGAAAA AATTCCACGA AGAAAAGAAA GCTTCGAAAG GTGAAGAGAA GAATGATGGG 
GTAGAGCAGA AATCTAAATC AGGAACCCCT TTTGAAAAGG AGGCAACTCC TGTACTACCT 
GCTAATGAAG CAGTTAAAGA TACGTTAACC GAAACATCTA ATGAAAAATC TACCTCTGAA 
GCGGAAAATA CTAAGAGATT ATTTTTAGAG CAGGTGAGAT TGCGCAAAGC TGCCATGGAA 
AGAAAGAAAA ACGGTTTAAT CTCCGAAACT GAGAAGAAGC AAGAAACTTC AAATCATGAT 
AATACTGATA CAACTAAGCC CAACTCGGTT ATTGAATCTG AACCTATTAA GGAAGCGCCG 
AAACCAACGG GAGAAGCTAA TGAAGTTGTG ATTGATGGAA AATCAGGGGC AAGTGTCAAA 
ACTCCACAAC ATGTAACTGG AAGTGTAACT AAATCCGTGA CTTTTAACGA ACCCGAGAAT 
GAGAGCAGTT CTCAGGATGT TGATGAGCTT GTTAAAGATG ATGATACTAC TGAAATTTCG 
GATACGACTG GTGGAAAAAC TGTAAATAAA AGTGACGACG AAACAATAAA TTCCGTAATC 
ACCACAGAGG AGAACACAGT GAAGGAGACA GAACCTTCCA CTTCCGATAT TGAGATGCCA 
ACTGTATCTC AACTACTTGA AACTTTAGGT AAGGCTCAAC CAATTTCTGA CATATATGAG 
TTTGCCTATC CGGAAAATGT TGAAAGGCCT GATATCAAAT ACAAGAAACC AAGCGTTAAG 
TACACTTATG GCCCTACCTT CTTACTGCAA TTCAAAGATA AACTAAAATT CAGGCCTGAT 
CCTGCGTGGG TTGAAGCTGT ATCCTCGAAA ATTGTTATAC CTCCTCATAT AGCCAGAAAT 
AAACCAAAAG ATAGTGGCAG ATTTGGAGGC GATTTCAGAA GTCCATCTAT GCGCGGTATG 
GACCATACTT CCAGTTCAAG AGTTTCATCG AAAAGAAGAT CAAAGAGAAT GGGTGACGAC 
AGAAGATCTA ATAGAGGATA TACCTCCAGA AAAGATCGCG AAAAAGCTGC AGAAAAGGCA 
GAAGAGCAAG CTCCAAAGGA AGAAATCGCC CCGTTGGTTC CGAGTGCTAA TAGATGGATA 
CCTAAATCAA GGGTTAAAAA AACAGAAAAG AAGTTAGCTC CTGACGGCAA AACTGAATTA 
TTTGACAAGG AAGAAGTAGA ACGAAAAATG AAGTCTCTAT TAAACAAGTT AACTTTAGAA 
ATGTTTGATT CAATCTCCTC TGAGATTTTG GATATAGCAA ACCAATCAAA GTGGGAAGAT 
GATGGCGAAA CATTGAAAAT AGTTATTGAA CAAATTTTCC ACAAGGCTTG TGATGAACCT 
CATTGGTCTT CAATGTATGC TCAGTTATGT GGCAAAGTGG TCAAAGACCT TGACCCAAAC 
ATTAAAGATA AAGAGAACGA AGGAAAGAAT GGACCAAAGC TTGTGTTGCA TTATTTAGTG 
GCAAGATGTC ACGAAGAATT TGAGAAAGGT TGGGCTGATA AGTTGCCTGC AGGAGAAGAT 
GGTAATCCAC TAGAACCTGA AATGATGTCC GATGAGTATT ATATTGCGGC TGCCGCCAAG 
AGAAGAGGGT TAGGTTTAGT ACGTTTCATA GGGTACTTAT ATTGTTTGAA CTTATTAACT 
GGTAAGATGA TGTTTGAGTG CTTCCGGAGA TTAATGAAAG ATCTAAACAA CGATCCATCC 
GAGGAGACAT TAGAGTCCGT TATTGAATTA CTGAATACTG TGGGTGAACA ATTTGAACAT 
GATAAATTTG TTACGCCTCA GGCAACTCTT GAAGGTTCGG TACTACTCGA CAATCTATTT 
ATGTTGTTAC AACATATCAT TGACGGTGGC ACCATATCGA ACAGAATAAA GTTCAAGTTG 
ATAGACGTGA AGGAATTGAG GGAAATTAAG CATTGGAACA GCGCTAAGAA AGACGCCGGA 
CCAAAGACAA TTCAACAAAT CCACCAAGAA GAAGAACAAT TACGTCAGAA AAAGAACAGT 
CAGAGATCAA ATTCTAGATT CAACAATCAC AACCAATCGA ACAGCAACAG ATATTCTTCT 
AACAGAAGGA ACATGCAAAA CACCCAAAGA GATAGTTTTG CTTCCACGAA AACAGGTTCT 
TTCAGAAATA ACCAAAGAAA TGCTCGTAAA GTCGAAGAAG TTTCTCAAGC TCCAAGAGCT 
AATATGTTCG ACGCATTAAT GAATAACGAT GGGGACAGTG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011466.1
CDS	1..2745
Translation	MTDQRGPPPPHPQQANGYKKFPPHDNQYSGANNSQPNNHYNENLYSAREPHNNKQYQSKNGKYGTNKYNNRNNSQGNAQYYNNRFNNGYRLNNNDYNPAMLPGMQWPANYYAPQMYYIPQQMVPVASPPYTHQPLNTNPEPPSTPKTTKIEITTKTGERLNLKKFHEEKKASKGEEKNDGVEQKSKSGTPFEKEATPVLPANEAVKDTLTETSNEKSTSEAENTKRLFLEQVRLRKAAMERKKNGLISETEKKQETSNHDNTDTTKPNSVIESEPIKEAPKPTGEANEVVIDGKSGASVKTPQHVTGSVTKSVTFNEPENESSSQDVDELVKDDDTTEISDTTGGKTVNKSDDETINSVITTEENTVKETEPSTSDIEMPTVSQLLETLGKAQPISDIYEFAYPENVERPDIKYKKPSVKYTYGPTFLLQFKDKLKFRPDPAWVEAVSSKIVIPPHIARNKPKDSGRFGGDFRSPSMRGMDHTSSSRVSSKRRSKRMGDDRRSNRGYTSRKDREKAAEKAEEQAPKEEIAPLVPSANRWIPKSRVKKTEKKLAPDGKTELFDKEEVERKMKSLLNKLTLEMFDSISSEILDIANQSKWEDDGETLKIVIEQIFHKACDEPHWSSMYAQLCGKVVKDLDPNIKDKENEGKNGPKLVLHYLVARCHEEFEKGWADKLPAGEDGNPLEPEMMSDEYYIAAAAKRRGLGLVRFIGYLYCLNLLTGKMMFECFRRLMKDLNNDPSEETLESVIELLNTVGEQFEHDKFVTPQATLEGSVLLDNLFMLLQHIIDGGTISNRIKFKLIDVKELREIKHWNSAKKDAGPKTIQQIHQEEEQLRQKKNSQRSNSRFNNHNQSNSNRYSSNRRNMQNTQRDSFASTKTGSFRNNQRNARKVEEVSQAPRANMFDALMNNDGDSD

Target ORF information:

RefSeq Version	NM_001180914.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C translation initiation factor eIF4G (TIF4632), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180914.1

ORF Insert Sequence:

ATGACTGACC AAAGAGGTCC ACCGCCCCCA CACCCGCAGC AAGCCAATGG CTACAAGAAA 
TTTCCTCCTC ATGATAACCA ATACTCTGGA GCCAATAATA GTCAGCCAAA TAACCACTAC 
AATGAAAATC TTTACAGTGC AAGGGAACCT CACAATAACA AGCAATACCA GTCGAAAAAT 
GGCAAATACG GGACAAATAA ATACAATAAT CGTAATAATA GTCAAGGAAA TGCACAGTAC 
TACAATAACA GATTCAATAA TGGCTACAGA CTGAATAACA ACGACTATAA CCCGGCAATG 
TTGCCAGGTA TGCAATGGCC AGCTAACTAC TATGCTCCTC AGATGTATTA TATACCCCAA 
CAAATGGTTC CAGTTGCTTC TCCACCATAT ACACACCAAC CACTCAACAC TAATCCCGAA 
CCTCCCTCTA CACCTAAAAC TACAAAAATA GAAATCACTA CGAAAACTGG TGAACGTTTA 
AATTTGAAAA AATTCCACGA AGAAAAGAAA GCTTCGAAAG GTGAAGAGAA GAATGATGGG 
GTAGAGCAGA AATCTAAATC AGGAACCCCT TTTGAAAAGG AGGCAACTCC TGTACTACCT 
GCTAATGAAG CAGTTAAAGA TACGTTAACC GAAACATCTA ATGAAAAATC TACCTCTGAA 
GCGGAAAATA CTAAGAGATT ATTTTTAGAG CAGGTGAGAT TGCGCAAAGC TGCCATGGAA 
AGAAAGAAAA ACGGTTTAAT CTCCGAAACT GAGAAGAAGC AAGAAACTTC AAATCATGAT 
AATACTGATA CAACTAAGCC CAACTCGGTT ATTGAATCTG AACCTATTAA GGAAGCGCCG 
AAACCAACGG GAGAAGCTAA TGAAGTTGTG ATTGATGGAA AATCAGGGGC AAGTGTCAAA 
ACTCCACAAC ATGTAACTGG AAGTGTAACT AAATCCGTGA CTTTTAACGA ACCCGAGAAT 
GAGAGCAGTT CTCAGGATGT TGATGAGCTT GTTAAAGATG ATGATACTAC TGAAATTTCG 
GATACGACTG GTGGAAAAAC TGTAAATAAA AGTGACGACG AAACAATAAA TTCCGTAATC 
ACCACAGAGG AGAACACAGT GAAGGAGACA GAACCTTCCA CTTCCGATAT TGAGATGCCA 
ACTGTATCTC AACTACTTGA AACTTTAGGT AAGGCTCAAC CAATTTCTGA CATATATGAG 
TTTGCCTATC CGGAAAATGT TGAAAGGCCT GATATCAAAT ACAAGAAACC AAGCGTTAAG 
TACACTTATG GCCCTACCTT CTTACTGCAA TTCAAAGATA AACTAAAATT CAGGCCTGAT 
CCTGCGTGGG TTGAAGCTGT ATCCTCGAAA ATTGTTATAC CTCCTCATAT AGCCAGAAAT 
AAACCAAAAG ATAGTGGCAG ATTTGGAGGC GATTTCAGAA GTCCATCTAT GCGCGGTATG 
GACCATACTT CCAGTTCAAG AGTTTCATCG AAAAGAAGAT CAAAGAGAAT GGGTGACGAC 
AGAAGATCTA ATAGAGGATA TACCTCCAGA AAAGATCGCG AAAAAGCTGC AGAAAAGGCA 
GAAGAGCAAG CTCCAAAGGA AGAAATCGCC CCGTTGGTTC CGAGTGCTAA TAGATGGATA 
CCTAAATCAA GGGTTAAAAA AACAGAAAAG AAGTTAGCTC CTGACGGCAA AACTGAATTA 
TTTGACAAGG AAGAAGTAGA ACGAAAAATG AAGTCTCTAT TAAACAAGTT AACTTTAGAA 
ATGTTTGATT CAATCTCCTC TGAGATTTTG GATATAGCAA ACCAATCAAA GTGGGAAGAT 
GATGGCGAAA CATTGAAAAT AGTTATTGAA CAAATTTTCC ACAAGGCTTG TGATGAACCT 
CATTGGTCTT CAATGTATGC TCAGTTATGT GGCAAAGTGG TCAAAGACCT TGACCCAAAC 
ATTAAAGATA AAGAGAACGA AGGAAAGAAT GGACCAAAGC TTGTGTTGCA TTATTTAGTG 
GCAAGATGTC ACGAAGAATT TGAGAAAGGT TGGGCTGATA AGTTGCCTGC AGGAGAAGAT 
GGTAATCCAC TAGAACCTGA AATGATGTCC GATGAGTATT ATATTGCGGC TGCCGCCAAG 
AGAAGAGGGT TAGGTTTAGT ACGTTTCATA GGGTACTTAT ATTGTTTGAA CTTATTAACT 
GGTAAGATGA TGTTTGAGTG CTTCCGGAGA TTAATGAAAG ATCTAAACAA CGATCCATCC 
GAGGAGACAT TAGAGTCCGT TATTGAATTA CTGAATACTG TGGGTGAACA ATTTGAACAT 
GATAAATTTG TTACGCCTCA GGCAACTCTT GAAGGTTCGG TACTACTCGA CAATCTATTT 
ATGTTGTTAC AACATATCAT TGACGGTGGC ACCATATCGA ACAGAATAAA GTTCAAGTTG 
ATAGACGTGA AGGAATTGAG GGAAATTAAG CATTGGAACA GCGCTAAGAA AGACGCCGGA 
CCAAAGACAA TTCAACAAAT CCACCAAGAA GAAGAACAAT TACGTCAGAA AAAGAACAGT 
CAGAGATCAA ATTCTAGATT CAACAATCAC AACCAATCGA ACAGCAACAG ATATTCTTCT 
AACAGAAGGA ACATGCAAAA CACCCAAAGA GATAGTTTTG CTTCCACGAA AACAGGTTCT 
TTCAGAAATA ACCAAAGAAA TGCTCGTAAA GTCGAAGAAG TTTCTCAAGC TCCAAGAGCT 
AATATGTTCG ACGCATTAAT GAATAACGAT GGGGACAGTG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TIF4632 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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