AFT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	AFT1
Entrez Gene ID	852809
Full Name	DNA-binding transcription factor AFT1
Synonyms	RCS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180936.1	NP_011444.1	DNA-binding transcription factor AFT1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, these results suggest that the protein-protein interaction between Sit1 and Aft1 is an important factor in the ferrioxamine B uptake activity of Sit1.
Title: Physical interaction between Sit1 and Aft1 upregulates FOB uptake activity by inhibiting protein degradation of Sit1 in Saccharomyces cerevisiae.

The findings suggest that Rad9 functions together with Aft1 on DNA damage-prone chromatin to facilitate genome surveillance, thereby ensuring rapid and effective response to possible DNA damage events.
Title: Rad9 interacts with Aft1 to facilitate genome surveillance in fragile genomic sites under non-DNA damage-inducing conditions in S. cerevisiae.

Aft1p dissociates from its target promoters under iron-replete conditions due to an interaction between Aft1p and the monothiol glutaredoxin Grx3p or Grx4p (Grx3/4p).
Title: Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression.

These data suggest that Aft1p and Mac1p might corporately regulate transcription of CTR2.
Title: Metal-sensing transcription factors Mac1p and Aft1p coordinately regulate vacuolar copper transporter CTR2 in Saccharomyces cerevisiae.

Aft1 associates with the kinetochore complex through Iml3.
Title: Iron-responsive transcription factor Aft1 interacts with kinetochore protein Iml3 and promotes pericentromeric cohesin.

The following AFT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02171	NM_001180936.1 Latest version!	Saccharomyces cerevisiae S288C DNA-binding transcription factor AFT1 (AFT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFT1 ( NM_001180936.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011444.1 Saccharomyces cerevisiae S288C DNA-binding transcription factor AFT1 (AFT1), partial mRNA.

Price & Availability↑

CloneID	OSi02171
Clone ID Related Accession (Same CDS sequence)	NM_001180936.1
Accession Version	NM_001180936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-binding transcription factor AFT1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAGGCT TCAATCCGGC TGACATAGAA CATGCGTCAC CGATTAATTC ATCTGACAGC 
CATTCATCCT CCTTTGTATA TGCTCTACCC AAAAGTGCTA GTGAATATGT AGTCAACCAT 
AATGAGGGTC GTGCAAGTGC AAGTGGAAAT CCAGCCGCAG TGCCGTCTCC CATAATGACA 
CTGAATCTCA AAAGCACACA TTCCCTCAAT ATTGATCAGC ATGTTCATAC CTCAACATCG 
CCGACGGAAA CTATTGGGCA TATTCATCAT GTGGAAAAGC TGAATCAAAA CAATTTGATT 
CATCTGGATC CAGTACCCAA CTTTGAAGAT AAGTCCGATA TTAAGCCTTG GTTGCAAAAG 
ATTTTTTATC CTCAAGGAAT AGAACTTGTG ATAGAAAGGT CGGACGCATT TAAAGTTGTC 
TTCAAGTGTA AAGCTGCTAA AAGGGGAAGG AACGCGAGAA GGAAAAGAAA AGATAAGCCC 
AAAGGACAGG ACCACGAAGA CGAGAAATCC AAGATCAATG ATGACGAATT AGAATATGCG 
AGTCCTTCTA ATGCCACAGT AACCAATGGG CCTCAAACAT CGCCCGATCA AACATCCTCC 
ATAAAGCCAA AGAAAAAAAG ATGTGTATCG AGGTTTAATA ACTGTCCGTT TAGAGTACGA 
GCTACTTATT CGTTAAAGAG GAAAAGATGG AGCATTGTTG TAATGGACAA TAACCATTCA 
CATCAGCTAA AGTTTAACCC TGATTCCGAA GAGTACAAAA AATTCAAAGA AAAATTAAGA 
AAGGATAATG ACGTAGATGC AATCAAGAAA TTCGACGAAT TGGAATACAG AACTTTGGCC 
AATTTGCCCA TTCCAACAGC TACAATCCCC TGTGATTGTG GTTTAACAAA TGAAATACAA 
AGTTTCAATG TCGTATTGCC CACTAACAGT AATGTTACTT CATCAGCATC CTCTTCAACT 
GTATCGTCCA TATCCCTTGA TTCATCGAAT GCATCTAAAA GGCCATGCTT ACCCTCTGTA 
AATAACACCG GTAGTATCAA TACCAATAAC GTAAGGAAAC CGAAAAGCCA GTGTAAGAAT 
AAAGACACAC TCTTAAAAAG AACCACCATG CAGAACTTTC TCACAACTAA ATCAAGGCTG 
CGTAAGACCG GTACGCCAAC ATCTTCGCAA CACTCATCTA CAGCATTTTC AGGATATATT 
GATGATCCTT TCAATTTGAA TGAAATCTTG CCACTGCCGG CATCCGATTT CAAGCTAAAC 
ACTGTAACAA ATTTGAACGA AATTGACTTT ACGAACATTT TTACCAAATC GCCGCATCCA 
CATAGCGGGT CTACCCATCC AAGACAAGTC TTCGACCAAT TGGACGATTG TTCCTCTATA 
CTCTTCTCTC CATTAACTAC AAACACGAAT AATGAATTTG AAGGAGAGTC AGATGATTTT 
GTTCATTCTC CATATTTGAA CTCAGAGGCA GATTTCAGCC AAATTCTTAG TAGTGCTCCC 
CCAGTCCATC ATGACCCAAA TGAAACACAT CAGGAAAACC AGGATATTAT TGATAGATTT 
GCTAATAGTT CCCAAGAACA TAATGAGTAT ATTCTACAAT ATTTGACGCA CTCCGATGCT 
GCTAACCACA ATAACATCGG CGTTCCAAAC AACAATTCAC ATTCGCTAAA TACTCAGCAT 
AACGTTTCTG ATCTGGGCAA CTCACTTTTA AGACAAGAAG CTTTAGTTGG CAGCTCTTCA 
ACAAAAATCT TCGACGAATT GAAATTTGTA CAAAATGGCC CACACGGTTC TCAACATCCT 
ATAGATTTTC AACATGTTGA CCATCGTCAT CTCAGCTCTA ATGAACCTCA AGTACGATCA 
CATCAATATG GTCCGCAACA GCAGCCACCG CAGCAATTGC AATATCACCA AAATCAGCCC 
CACGACGGCC ATAACCACGA ACAGCACCAA ACAGTACAAA AGGATATGCA AACGCATGAA 
TCGCTAGAAA TAATGGGAAA CACATTATTG GAAGAGTTCA AAGACATTAA AATGGTGAAC 
GGCGAGTTGA AGTATGTGAA GCCAGAAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011444.1
CDS	1..2073
Translation	MEGFNPADIEHASPINSSDSHSSSFVYALPKSASEYVVNHNEGRASASGNPAAVPSPIMTLNLKSTHSLNIDQHVHTSTSPTETIGHIHHVEKLNQNNLIHLDPVPNFEDKSDIKPWLQKIFYPQGIELVIERSDAFKVVFKCKAAKRGRNARRKRKDKPKGQDHEDEKSKINDDELEYASPSNATVTNGPQTSPDQTSSIKPKKKRCVSRFNNCPFRVRATYSLKRKRWSIVVMDNNHSHQLKFNPDSEEYKKFKEKLRKDNDVDAIKKFDELEYRTLANLPIPTATIPCDCGLTNEIQSFNVVLPTNSNVTSSASSSTVSSISLDSSNASKRPCLPSVNNTGSINTNNVRKPKSQCKNKDTLLKRTTMQNFLTTKSRLRKTGTPTSSQHSSTAFSGYIDDPFNLNEILPLPASDFKLNTVTNLNEIDFTNIFTKSPHPHSGSTHPRQVFDQLDDCSSILFSPLTTNTNNEFEGESDDFVHSPYLNSEADFSQILSSAPPVHHDPNETHQENQDIIDRFANSSQEHNEYILQYLTHSDAANHNNIGVPNNNSHSLNTQHNVSDLGNSLLRQEALVGSSSTKIFDELKFVQNGPHGSQHPIDFQHVDHRHLSSNEPQVRSHQYGPQQQPPQQLQYHQNQPHDGHNHEQHQTVQKDMQTHESLEIMGNTLLEEFKDIKMVNGELKYVKPED

Target ORF information:

RefSeq Version	NM_001180936.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-binding transcription factor AFT1 (AFT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180936.1

ORF Insert Sequence:

ATGGAAGGCT TCAATCCGGC TGACATAGAA CATGCGTCAC CGATTAATTC ATCTGACAGC 
CATTCATCCT CCTTTGTATA TGCTCTACCC AAAAGTGCTA GTGAATATGT AGTCAACCAT 
AATGAGGGTC GTGCAAGTGC AAGTGGAAAT CCAGCCGCAG TGCCGTCTCC CATAATGACA 
CTGAATCTCA AAAGCACACA TTCCCTCAAT ATTGATCAGC ATGTTCATAC CTCAACATCG 
CCGACGGAAA CTATTGGGCA TATTCATCAT GTGGAAAAGC TGAATCAAAA CAATTTGATT 
CATCTGGATC CAGTACCCAA CTTTGAAGAT AAGTCCGATA TTAAGCCTTG GTTGCAAAAG 
ATTTTTTATC CTCAAGGAAT AGAACTTGTG ATAGAAAGGT CGGACGCATT TAAAGTTGTC 
TTCAAGTGTA AAGCTGCTAA AAGGGGAAGG AACGCGAGAA GGAAAAGAAA AGATAAGCCC 
AAAGGACAGG ACCACGAAGA CGAGAAATCC AAGATCAATG ATGACGAATT AGAATATGCG 
AGTCCTTCTA ATGCCACAGT AACCAATGGG CCTCAAACAT CGCCCGATCA AACATCCTCC 
ATAAAGCCAA AGAAAAAAAG ATGTGTATCG AGGTTTAATA ACTGTCCGTT TAGAGTACGA 
GCTACTTATT CGTTAAAGAG GAAAAGATGG AGCATTGTTG TAATGGACAA TAACCATTCA 
CATCAGCTAA AGTTTAACCC TGATTCCGAA GAGTACAAAA AATTCAAAGA AAAATTAAGA 
AAGGATAATG ACGTAGATGC AATCAAGAAA TTCGACGAAT TGGAATACAG AACTTTGGCC 
AATTTGCCCA TTCCAACAGC TACAATCCCC TGTGATTGTG GTTTAACAAA TGAAATACAA 
AGTTTCAATG TCGTATTGCC CACTAACAGT AATGTTACTT CATCAGCATC CTCTTCAACT 
GTATCGTCCA TATCCCTTGA TTCATCGAAT GCATCTAAAA GGCCATGCTT ACCCTCTGTA 
AATAACACCG GTAGTATCAA TACCAATAAC GTAAGGAAAC CGAAAAGCCA GTGTAAGAAT 
AAAGACACAC TCTTAAAAAG AACCACCATG CAGAACTTTC TCACAACTAA ATCAAGGCTG 
CGTAAGACCG GTACGCCAAC ATCTTCGCAA CACTCATCTA CAGCATTTTC AGGATATATT 
GATGATCCTT TCAATTTGAA TGAAATCTTG CCACTGCCGG CATCCGATTT CAAGCTAAAC 
ACTGTAACAA ATTTGAACGA AATTGACTTT ACGAACATTT TTACCAAATC GCCGCATCCA 
CATAGCGGGT CTACCCATCC AAGACAAGTC TTCGACCAAT TGGACGATTG TTCCTCTATA 
CTCTTCTCTC CATTAACTAC AAACACGAAT AATGAATTTG AAGGAGAGTC AGATGATTTT 
GTTCATTCTC CATATTTGAA CTCAGAGGCA GATTTCAGCC AAATTCTTAG TAGTGCTCCC 
CCAGTCCATC ATGACCCAAA TGAAACACAT CAGGAAAACC AGGATATTAT TGATAGATTT 
GCTAATAGTT CCCAAGAACA TAATGAGTAT ATTCTACAAT ATTTGACGCA CTCCGATGCT 
GCTAACCACA ATAACATCGG CGTTCCAAAC AACAATTCAC ATTCGCTAAA TACTCAGCAT 
AACGTTTCTG ATCTGGGCAA CTCACTTTTA AGACAAGAAG CTTTAGTTGG CAGCTCTTCA 
ACAAAAATCT TCGACGAATT GAAATTTGTA CAAAATGGCC CACACGGTTC TCAACATCCT 
ATAGATTTTC AACATGTTGA CCATCGTCAT CTCAGCTCTA ATGAACCTCA AGTACGATCA 
CATCAATATG GTCCGCAACA GCAGCCACCG CAGCAATTGC AATATCACCA AAATCAGCCC 
CACGACGGCC ATAACCACGA ACAGCACCAA ACAGTACAAA AGGATATGCA AACGCATGAA 
TCGCTAGAAA TAATGGGAAA CACATTATTG GAAGAGTTCA AAGACATTAA AATGGTGAAC 
GGCGAGTTGA AGTATGTGAA GCCAGAAGAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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AFT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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