HSF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HSF1
Entrez Gene ID	852806
Full Name	stress-responsive transcription factor HSF1
Synonyms	EXA3,MAS3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180938.3	NP_011442.3	stress-responsive transcription factor HSF1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies suggest that heat shock factor 1 (Hsf1) controls a set of chaperone protein genes devoted to restoring protein-folding homeostasis in heat shock response.
Title: Size doesn't matter in the heat shock response.

A characteristic residence time for non-specific interactions of HSF1 transcription factor with chromatin
Title: Single molecule tracking of Ace1p in Saccharomyces cerevisiae defines a characteristic residence time for non-specific interactions of transcription factors with chromatin.

Here the authors show that in budding yeast, Hsf1 basally associates with the chaperone Hsp70 and this association is transiently disrupted by heat shock, providing the first evidence that a chaperone repressor directly regulates Hsf1 activity.
Title: Dynamic control of Hsf1 during heat shock by a chaperone switch and phosphorylation.

Yeast chaperone gene expression is an essential housekeeping mechanism and provides a roadmap for defining the function of HSF1 as a driver of oncogenesis.
Title: Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis.

regulation of Hsf1-Skn7 activities by the catalytic subunits of PKA
Title: Complex regulation of Hsf1-Skn7 activities by the catalytic subunits of PKA in Saccharomyces cerevisiae: experimental and computational evidences.

The following HSF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02170	NM_001180938.3 Latest version!	Saccharomyces cerevisiae S288C stress-responsive transcription factor HSF1 (HSF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSF1 ( NM_001180938.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011442.3 Saccharomyces cerevisiae S288C stress-responsive transcription factor HSF1 (HSF1), partial mRNA.

Price & Availability↑

CloneID	OSi02170
Clone ID Related Accession (Same CDS sequence)	NM_001180938.3
Accession Version	NM_001180938.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	stress-responsive transcription factor HSF1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001180938.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATAATG CTGCAAATAC AGGGACGACC AATGAGTCAA ACGTGAGCGA TGCTCCCCGT 
ATTGAGCCTT TACCAAGCTT GAATGATGAT GACATTGAAA AAATCTTACA ACCGAACGAT 
ATCTTTACGA CCGATCGTAC CGATGCAAGT ACTACATCTT CCACAGCCAT TGAAGATATT 
ATTAACCCCT CATTGGATCC GCAGTCAGCA GCATCGCCGG TTCCTTCTTC CTCTTTTTTC 
CATGACTCAA GGAAACCTTC CACCAGTACA CATTTAGTAA GGAGAGGTAC TCCATTGGGA 
ATTTACCAAA CCAATCTATA CGGTCACAAT AGCAGAGAAA ATACTAATCC TAATAGTACA 
TTATTATCTT CTAAGTTACT CGCGCATCCA CCAGTTCCTT ATGGGCAAAA TCCCGATTTA 
CTACAACATG CTGTGTACAG GGCACAGCCG TCAAGTGGAA CCACTAACGC GCAACCGCGC 
CAAACCACAA GAAGATATCA ATCCCATAAA TCACGGCCTG CATTTGTTAA TAAACTATGG 
AGCATGTTAA ACGATGATTC TAATACGAAA CTTATACAGT GGGCGGAGGA TGGAAAATCT 
TTTATTGTCA CGAATAGGGA GGAATTTGTG CACCAAATTT TACCAAAATA TTTTAAACAT 
TCCAATTTCG CTTCCTTTGT AAGACAATTG AACATGTATG GATGGCATAA AGTTCAAGAT 
GTCAAGTCAG GATCAATTCA AAGTAGTTCA GATGATAAGT GGCAATTTGA AAATGAAAAC 
TTCATTAGAG GTAGAGAAGA TTTGCTGGAA AAAATAATCA GGCAGAAAGG TTCCTCCAAT 
AACCATAATA GCCCTAGTGG TAACGGTAAT CCAGCGAATG GTAGCAACAT CCCTCTGGAC 
AATGCCGCAG GAAGTAATAA TAGCAATAAT AACATCAGTA GTAGTAATTC ATTTTTTAAC 
AATGGTCATT TATTGCAGGG TAAAACACTA AGATTAATGA ACGAAGCGAA TCTTGGAGAT 
AAGAATGATG TCACCGCGAT TTTGGGGGAA TTAGAGCAAA TAAAATATAA CCAGATTGCA 
ATTTCCAAAG ATTTACTAAG AATAAACAAA GATAATGAGT TATTATGGCA AGAGAATATG 
ATGGCCAGGG AAAGACATAG AACCCAACAG CAAGCCTTGG AAAAAATGTT CAGATTCTTG 
ACATCTATAG TCCCACACTT AGATCCCAAA ATGATTATGG ACGGGCTGGG AGATCCGAAA 
GTTAATAATG AAAAGCTAAA CAGTGCGAAT AACATTGGGT TAAATCGCGA CAACACAGGC 
ACTATAGATG AACTAAAATC CAACGATTCT TTCATAAACG ATGATCGTAA TTCTTTCACC 
AATGCTACAA CCAACGCCCG TAATAACATG AGTCCCAACA ATGATGACAA TAGTATTGAC 
ACCGCTAGCA CTAATACCAC CAACAGAAAG AAAAATATAG ATGAAAACAT CAAAAATAAC 
AACGACATAA TTAATGACAT TATATTTAAT ACCAACCTTG CCAACAATCT CAGCAATTAC 
AATTCCAACA ATAATGCTGG CTCGCCAATA AGGCCCTATA AACAAAGATA TCTTTTGAAA 
AATAGAGCCA ATTCCTCGAC ATCGAGTGAG AATCCAAGCC TAACGCCCTT TGATATCGAA 
TCTAATAATG ACCGCAAAAT TTCAGAAATT CCTTTTGATG ACGAAGAAGA AGAAGAAACG 
GATTTTAGGC CTTTTACCTC GCGAGATCCT AATAACCAAA CGAGTGAAAA CACTTTTGAT 
CCAAACAGAT TTACGATGCT CTCTGATGAT GATTTAAAAA AAGATTCTCA TACCAATGAC 
AATAAACACA ACGAAAGTGA TCTTTTTTGG GACAACGTAC ATAGAAATAT AGACGAACAA 
GATGCAAGAC TCCAGAACTT GGAAAATATG GTTCACATAC TTTCTCCTGG ATATCCTAAT 
AAGTCGTTCA ACAACAAAAC TTCCTCGACA AACACTAATT CCAATATGGA AAGTGCTGTC 
AACGTTAATA GCCCTGGTTT CAACTTACAG GATTATTTAA CTGGAGAGTC TAATTCCCCC 
AATTCTGTTC ATTCTGTTCC CTCCAATGGC AGCGGCTCCA CACCGTTGCC CATGCCAAAT 
GATAATGACA CCGAGCACGC AAGTACAAGT GTCAATCAAG GCGAAAATGG AAGCGGATTA 
ACGCCCTTCC TCACGGTAGA TGATCACACA CTAAACGACA ATAACACTAG TGAGGGAAGT 
ACAAGGGTGT CCCCCGATAT AAAGTTCAGC GCCACTGAAA ACACTAAAGT GAGTGATAAC 
CTGCCAAGCT TTAATGACCA CAGTTATTCC ACCCAGGCCG ACACGGCGCC CGAGAACGCT 
AAGAAAAGAT TTGTGGAGGA AATACCGGAA CCGGCTATAG TCGAAATACA GGACCCGACA 
GAGTACAACG ATCACCGCCT GCCCAAACGA GCTAAGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011442.3
CDS	1..2502
Translation	MNNAANTGTTNESNVSDAPRIEPLPSLNDDDIEKILQPNDIFTTDRTDASTTSSTAIEDIINPSLDPQSAASPVPSSSFFHDSRKPSTSTHLVRRGTPLGIYQTNLYGHNSRENTNPNSTLLSSKLLAHPPVPYGQNPDLLQHAVYRAQPSSGTTNAQPRQTTRRYQSHKSRPAFVNKLWSMLNDDSNTKLIQWAEDGKSFIVTNREEFVHQILPKYFKHSNFASFVRQLNMYGWHKVQDVKSGSIQSSSDDKWQFENENFIRGREDLLEKIIRQKGSSNNHNSPSGNGNPANGSNIPLDNAAGSNNSNNNISSSNSFFNNGHLLQGKTLRLMNEANLGDKNDVTAILGELEQIKYNQIAISKDLLRINKDNELLWQENMMARERHRTQQQALEKMFRFLTSIVPHLDPKMIMDGLGDPKVNNEKLNSANNIGLNRDNTGTIDELKSNDSFINDDRNSFTNATTNARNNMSPNNDDNSIDTASTNTTNRKKNIDENIKNNNDIINDIIFNTNLANNLSNYNSNNNAGSPIRPYKQRYLLKNRANSSTSSENPSLTPFDIESNNDRKISEIPFDDEEEEETDFRPFTSRDPNNQTSENTFDPNRFTMLSDDDLKKDSHTNDNKHNESDLFWDNVHRNIDEQDARLQNLENMVHILSPGYPNKSFNNKTSSTNTNSNMESAVNVNSPGFNLQDYLTGESNSPNSVHSVPSNGSGSTPLPMPNDNDTEHASTSVNQGENGSGLTPFLTVDDHTLNDNNTSEGSTRVSPDIKFSATENTKVSDNLPSFNDHSYSTQADTAPENAKKRFVEEIPEPAIVEIQDPTEYNDHRLPKRAKK

Target ORF information:

RefSeq Version	NM_001180938.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C stress-responsive transcription factor HSF1 (HSF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180938.3

ORF Insert Sequence:

ATGAATAATG CTGCAAATAC AGGGACGACC AATGAGTCAA ACGTGAGCGA TGCTCCCCGT 
ATTGAGCCTT TACCAAGCTT GAATGATGAT GACATTGAAA AAATCTTACA ACCGAACGAT 
ATCTTTACGA CCGATCGTAC CGATGCAAGT ACTACATCTT CCACAGCCAT TGAAGATATT 
ATTAACCCCT CATTGGATCC GCAGTCAGCA GCATCGCCGG TTCCTTCTTC CTCTTTTTTC 
CATGACTCAA GGAAACCTTC CACCAGTACA CATTTAGTAA GGAGAGGTAC TCCATTGGGA 
ATTTACCAAA CCAATCTATA CGGTCACAAT AGCAGAGAAA ATACTAATCC TAATAGTACA 
TTATTATCTT CTAAGTTACT CGCGCATCCA CCAGTTCCTT ATGGGCAAAA TCCCGATTTA 
CTACAACATG CTGTGTACAG GGCACAGCCG TCAAGTGGAA CCACTAACGC GCAACCGCGC 
CAAACCACAA GAAGATATCA ATCCCATAAA TCACGGCCTG CATTTGTTAA TAAACTATGG 
AGCATGTTAA ACGATGATTC TAATACGAAA CTTATACAGT GGGCGGAGGA TGGAAAATCT 
TTTATTGTCA CGAATAGGGA GGAATTTGTG CACCAAATTT TACCAAAATA TTTTAAACAT 
TCCAATTTCG CTTCCTTTGT AAGACAATTG AACATGTATG GATGGCATAA AGTTCAAGAT 
GTCAAGTCAG GATCAATTCA AAGTAGTTCA GATGATAAGT GGCAATTTGA AAATGAAAAC 
TTCATTAGAG GTAGAGAAGA TTTGCTGGAA AAAATAATCA GGCAGAAAGG TTCCTCCAAT 
AACCATAATA GCCCTAGTGG TAACGGTAAT CCAGCGAATG GTAGCAACAT CCCTCTGGAC 
AATGCCGCAG GAAGTAATAA TAGCAATAAT AACATCAGTA GTAGTAATTC ATTTTTTAAC 
AATGGTCATT TATTGCAGGG TAAAACACTA AGATTAATGA ACGAAGCGAA TCTTGGAGAT 
AAGAATGATG TCACCGCGAT TTTGGGGGAA TTAGAGCAAA TAAAATATAA CCAGATTGCA 
ATTTCCAAAG ATTTACTAAG AATAAACAAA GATAATGAGT TATTATGGCA AGAGAATATG 
ATGGCCAGGG AAAGACATAG AACCCAACAG CAAGCCTTGG AAAAAATGTT CAGATTCTTG 
ACATCTATAG TCCCACACTT AGATCCCAAA ATGATTATGG ACGGGCTGGG AGATCCGAAA 
GTTAATAATG AAAAGCTAAA CAGTGCGAAT AACATTGGGT TAAATCGCGA CAACACAGGC 
ACTATAGATG AACTAAAATC CAACGATTCT TTCATAAACG ATGATCGTAA TTCTTTCACC 
AATGCTACAA CCAACGCCCG TAATAACATG AGTCCCAACA ATGATGACAA TAGTATTGAC 
ACCGCTAGCA CTAATACCAC CAACAGAAAG AAAAATATAG ATGAAAACAT CAAAAATAAC 
AACGACATAA TTAATGACAT TATATTTAAT ACCAACCTTG CCAACAATCT CAGCAATTAC 
AATTCCAACA ATAATGCTGG CTCGCCAATA AGGCCCTATA AACAAAGATA TCTTTTGAAA 
AATAGAGCCA ATTCCTCGAC ATCGAGTGAG AATCCAAGCC TAACGCCCTT TGATATCGAA 
TCTAATAATG ACCGCAAAAT TTCAGAAATT CCTTTTGATG ACGAAGAAGA AGAAGAAACG 
GATTTTAGGC CTTTTACCTC GCGAGATCCT AATAACCAAA CGAGTGAAAA CACTTTTGAT 
CCAAACAGAT TTACGATGCT CTCTGATGAT GATTTAAAAA AAGATTCTCA TACCAATGAC 
AATAAACACA ACGAAAGTGA TCTTTTTTGG GACAACGTAC ATAGAAATAT AGACGAACAA 
GATGCAAGAC TCCAGAACTT GGAAAATATG GTTCACATAC TTTCTCCTGG ATATCCTAAT 
AAGTCGTTCA ACAACAAAAC TTCCTCGACA AACACTAATT CCAATATGGA AAGTGCTGTC 
AACGTTAATA GCCCTGGTTT CAACTTACAG GATTATTTAA CTGGAGAGTC TAATTCCCCC 
AATTCTGTTC ATTCTGTTCC CTCCAATGGC AGCGGCTCCA CACCGTTGCC CATGCCAAAT 
GATAATGACA CCGAGCACGC AAGTACAAGT GTCAATCAAG GCGAAAATGG AAGCGGATTA 
ACGCCCTTCC TCACGGTAGA TGATCACACA CTAAACGACA ATAACACTAG TGAGGGAAGT 
ACAAGGGTGT CCCCCGATAT AAAGTTCAGC GCCACTGAAA ACACTAAAGT GAGTGATAAC 
CTGCCAAGCT TTAATGACCA CAGTTATTCC ACCCAGGCCG ACACGGCGCC CGAGAACGCT 
AAGAAAAGAT TTGTGGAGGA AATACCGGAA CCGGCTATAG TCGAAATACA GGACCCGACA 
GAGTACAACG ATCACCGCCT GCCCAAACGA GCTAAGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HSF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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