MAD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MAD1
Entrez Gene ID	852794
Full Name	coiled-coil domain-containing protein MAD1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180951.3	NP_011429.3	coiled-coil domain-containing protein MAD1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MAD1	NP_011429.3
Kluyveromyces lactis	KLLA0E19141g	XP_454817.1
Schizosaccharomyces pombe (fission yeast)	mad1	NP_595516.1
Neurospora crassa	NCU04428	XP_957505.2
Magnaporthe oryzae (rice blast fungus)	MGG_04908	XP_003712395.1
Eremothecium gossypii	AGOS_AFL065C	NP_985483.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mad1 recruitment to the kinetochore and it's role in the spindle assembly checkpoint
Title: Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.

Mad1 is recruited by detached kinetochores competing with alternate other sites in nucleus: Mad1 pool is small enough to be fully depleted by one pair of detached kinetochores. Lateral attachment licenses Mad1 removal from kinetochores after kinetic delay
Title: Control of the spindle checkpoint by lateral kinetochore attachment and limited Mad1 recruitment.

Mad1p senses unattached kinetochores and, by altering nuclear pore complex transport activity, regulates the nuclear environment of the spindle.
Title: Mitosis-specific regulation of nuclear transport by the spindle assembly checkpoint protein Mad1p.

cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension.
Title: Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis.

Only the C terminus is required for spindle assembly checkpoint (SAC) function and the nuclear pore complex, through Nup53p, may act to regulate the duration of the SAC response.
Title: Interactions between Mad1p and the nuclear transport machinery in the yeast Saccharomyces cerevisiae.

The following MAD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02158	NM_001180951.3 Latest version!	Saccharomyces cerevisiae S288C coiled-coil domain-containing protein MAD1 (MAD1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAD1 ( NM_001180951.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011429.3 Saccharomyces cerevisiae S288C coiled-coil domain-containing protein MAD1 (MAD1), partial mRNA.

Price & Availability↑

CloneID	OSi02158
Clone ID Related Accession (Same CDS sequence)	NM_001180951.3
Accession Version	NM_001180951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	coiled-coil domain-containing protein MAD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001180951.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGTGA GAGCGGCATT GCAATGTTTC TTCAGTGCAT TGAGTGGACG ATTCACTGGA 
AAAAAATTGG GACTAGAGAT CTATTCTATC CAGTACAAAA TGAGCAATAG TGGTGGGTCC 
TCTCCTTTCT TGGAAAGCCC TGGAGGTTCG CCCGACGTAG GCAGTACAAA TGGACAAAGT 
AATCGTCAGA TTCAGGCGTT ACAATTCAAG CTCAATACTC TACAAAATGA GTATGAGATT 
GAAAAATTGC AGCTACAAAA ACAGACGAAT ATCTTAGAAA AGAAATATAA GGCCACGATT 
GACGAATTAG AGAAAGCATT GAATGATACA AAATATCTTT ATGAAAGCAA TGATAAATTG 
GAGCAAGAGT TAAAGAGTTT GAAAGAAAGA TCAGCTAATT CGATGAATGA TAAAGACAAA 
TGCATAGAGG AATTGCGAAC AACCCTCCAA AACAAAGATT TAGAAATGGA GACTTTAAGG 
CAGCAGTATG ATTCGAAGTT ATCAAAGGTC ACTAACCAAT GTGACCATTT CAAGTTGGAA 
GCAGAGAGCT CTCATTCGCT ACTGATGAAA TATGAGAAAG AAATAAAGAG GCAAAGTGTG 
GATATCAAAG ATTTGCAGCA TCAAGTGATG GAAAAGGATG ATGAATTATC TTCGGTAAAA 
GCATCTAAGA TGATAAACTC ACATCCAAAC TACTCCACCG AAGAGTTCAA CGAGCTCACT 
GAAATGAACA AAATGATCCA AGACCAAGTT CAGTATACTA AAGAATTGGA ACTAGCGAAC 
ATGCAACAAG CCAATGAATT GAAAAAACTT AAACAATCCC AGGACACTTC TACTTTTTGG 
AAATTAGAAA ATGAAAAACT ACAAAACAAA TTAAGTCAAT TACACGTACT CGAAAGTCAA 
TATGAAAATC TTCAATTGGA AAACATAGAT CTAAAATCGA AATTAACCAA ATGGGAAATT 
TATAATGATA GTGACGATGA TGATGATAAC AATGTTAACA ACAATGATAA TAATAATAAT 
AATAAAAATG ATAATAATAA TGATAATAAT AATGATACAA GTAATAACAA CAATATTAAT 
AATAACAACA GAACCAAAAA TAACATAAGA AATAACCCTG AAGAGATTAT CCGGGACTGG 
AAACTTACCA AAAAGGAATG TTTAATTTTG ACAGATATGA ATGATAAGTT AAGATTGGAC 
AATAACAATT TAAAGCTACT GAATGATGAA ATGGCTTTAG AGAGAAACCA AATACTAGAT 
TTGAACAAGA ATTATGAAAA CAACATAGTA AATTTGAAAA GATTAAATCA CGAGTTAGAA 
CAACAGAAGA GTTTATCTTT CGAAGAATGT CGATTACTGC GCGAACAATT AGATGGCCTA 
TATTCCGCAC AAAATAATGC TTTACTAGAG GTCGAAAATA GTGAAACACA TGCGTCAAAC 
AAGAACGTAA ACGAAGATAT GAATAATTTG ATCGATACTT ATAAAAATAA GACAGAAGAT 
TTAACAAACG AACTAAAGAA GCTAAACGAT CAGTTATTAT CAAATTCCAA TGATGTAGAA 
ACACAAAGAA AAAAGAGGAA GTTGACAAGT GACCAAATTG GTTTAAATTA TTCACAGAGG 
CTCAACGAAT TGCAACTGGA AAATGTAAGT GTCTCGAGGG AGTTGAGTAA AGCACAAACT 
ACAATTCAGC TACTACAAGA AAAATTAGAA AAGTTAACTA AACTAAAGGA GAAAAAAATA 
CGTATACTGC AATTACGTGA CGGCCCCTTC ATAAAAGATC AATTTATCAA GAAAAACAAA 
TTACTTCTTC TAGAAAAGGA AAATGCAGAT TTACTTAACG AATTGAAAAA GAATAATCCA 
GCAGTGGAAA CAGTACCCAT TTCTGTTTAT GATTCACTAA ATTTCGAGTT GAAACAGTTC 
GAACAGGAAG TTTTTAAGTC AAATAAAAGA TTTTCCAGAC TAAAGCAAGT TTTCAATAAT 
AAATCTTTGG AGTTTATTGA CGTTGTAAAC TCATTATTAG GTTTTAAATT GGAATTTCAA 
CAAGATAGTC GTGTGAAAAT ATTTTCCTGT TTCAAACCTG AAAAATATTT GATTGCTGAT 
CTAAATGAAA ACACTTTGAA ATCTAACCTT GATGCAGATA TAGAAGGATG GGATGATCTC 
ATGAATCTAT GGGTGGAAGA TAGAGGTCAA CTTCCGTGCT TTTTGGCAAC AATAACATTG 
CGTCTGTGGG AACAGCGACA AGCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011429.3
CDS	1..2250
Translation	MDVRAALQCFFSALSGRFTGKKLGLEIYSIQYKMSNSGGSSPFLESPGGSPDVGSTNGQSNRQIQALQFKLNTLQNEYEIEKLQLQKQTNILEKKYKATIDELEKALNDTKYLYESNDKLEQELKSLKERSANSMNDKDKCIEELRTTLQNKDLEMETLRQQYDSKLSKVTNQCDHFKLEAESSHSLLMKYEKEIKRQSVDIKDLQHQVMEKDDELSSVKASKMINSHPNYSTEEFNELTEMNKMIQDQVQYTKELELANMQQANELKKLKQSQDTSTFWKLENEKLQNKLSQLHVLESQYENLQLENIDLKSKLTKWEIYNDSDDDDDNNVNNNDNNNNNKNDNNNDNNNDTSNNNNINNNNRTKNNIRNNPEEIIRDWKLTKKECLILTDMNDKLRLDNNNLKLLNDEMALERNQILDLNKNYENNIVNLKRLNHELEQQKSLSFEECRLLREQLDGLYSAQNNALLEVENSETHASNKNVNEDMNNLIDTYKNKTEDLTNELKKLNDQLLSNSNDVETQRKKRKLTSDQIGLNYSQRLNELQLENVSVSRELSKAQTTIQLLQEKLEKLTKLKEKKIRILQLRDGPFIKDQFIKKNKLLLLEKENADLLNELKKNNPAVETVPISVYDSLNFELKQFEQEVFKSNKRFSRLKQVFNNKSLEFIDVVNSLLGFKLEFQQDSRVKIFSCFKPEKYLIADLNENTLKSNLDADIEGWDDLMNLWVEDRGQLPCFLATITLRLWEQRQAK

Target ORF information:

RefSeq Version	NM_001180951.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C coiled-coil domain-containing protein MAD1 (MAD1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180951.3

ORF Insert Sequence:

ATGGATGTGA GAGCGGCATT GCAATGTTTC TTCAGTGCAT TGAGTGGACG ATTCACTGGA 
AAAAAATTGG GACTAGAGAT CTATTCTATC CAGTACAAAA TGAGCAATAG TGGTGGGTCC 
TCTCCTTTCT TGGAAAGCCC TGGAGGTTCG CCCGACGTAG GCAGTACAAA TGGACAAAGT 
AATCGTCAGA TTCAGGCGTT ACAATTCAAG CTCAATACTC TACAAAATGA GTATGAGATT 
GAAAAATTGC AGCTACAAAA ACAGACGAAT ATCTTAGAAA AGAAATATAA GGCCACGATT 
GACGAATTAG AGAAAGCATT GAATGATACA AAATATCTTT ATGAAAGCAA TGATAAATTG 
GAGCAAGAGT TAAAGAGTTT GAAAGAAAGA TCAGCTAATT CGATGAATGA TAAAGACAAA 
TGCATAGAGG AATTGCGAAC AACCCTCCAA AACAAAGATT TAGAAATGGA GACTTTAAGG 
CAGCAGTATG ATTCGAAGTT ATCAAAGGTC ACTAACCAAT GTGACCATTT CAAGTTGGAA 
GCAGAGAGCT CTCATTCGCT ACTGATGAAA TATGAGAAAG AAATAAAGAG GCAAAGTGTG 
GATATCAAAG ATTTGCAGCA TCAAGTGATG GAAAAGGATG ATGAATTATC TTCGGTAAAA 
GCATCTAAGA TGATAAACTC ACATCCAAAC TACTCCACCG AAGAGTTCAA CGAGCTCACT 
GAAATGAACA AAATGATCCA AGACCAAGTT CAGTATACTA AAGAATTGGA ACTAGCGAAC 
ATGCAACAAG CCAATGAATT GAAAAAACTT AAACAATCCC AGGACACTTC TACTTTTTGG 
AAATTAGAAA ATGAAAAACT ACAAAACAAA TTAAGTCAAT TACACGTACT CGAAAGTCAA 
TATGAAAATC TTCAATTGGA AAACATAGAT CTAAAATCGA AATTAACCAA ATGGGAAATT 
TATAATGATA GTGACGATGA TGATGATAAC AATGTTAACA ACAATGATAA TAATAATAAT 
AATAAAAATG ATAATAATAA TGATAATAAT AATGATACAA GTAATAACAA CAATATTAAT 
AATAACAACA GAACCAAAAA TAACATAAGA AATAACCCTG AAGAGATTAT CCGGGACTGG 
AAACTTACCA AAAAGGAATG TTTAATTTTG ACAGATATGA ATGATAAGTT AAGATTGGAC 
AATAACAATT TAAAGCTACT GAATGATGAA ATGGCTTTAG AGAGAAACCA AATACTAGAT 
TTGAACAAGA ATTATGAAAA CAACATAGTA AATTTGAAAA GATTAAATCA CGAGTTAGAA 
CAACAGAAGA GTTTATCTTT CGAAGAATGT CGATTACTGC GCGAACAATT AGATGGCCTA 
TATTCCGCAC AAAATAATGC TTTACTAGAG GTCGAAAATA GTGAAACACA TGCGTCAAAC 
AAGAACGTAA ACGAAGATAT GAATAATTTG ATCGATACTT ATAAAAATAA GACAGAAGAT 
TTAACAAACG AACTAAAGAA GCTAAACGAT CAGTTATTAT CAAATTCCAA TGATGTAGAA 
ACACAAAGAA AAAAGAGGAA GTTGACAAGT GACCAAATTG GTTTAAATTA TTCACAGAGG 
CTCAACGAAT TGCAACTGGA AAATGTAAGT GTCTCGAGGG AGTTGAGTAA AGCACAAACT 
ACAATTCAGC TACTACAAGA AAAATTAGAA AAGTTAACTA AACTAAAGGA GAAAAAAATA 
CGTATACTGC AATTACGTGA CGGCCCCTTC ATAAAAGATC AATTTATCAA GAAAAACAAA 
TTACTTCTTC TAGAAAAGGA AAATGCAGAT TTACTTAACG AATTGAAAAA GAATAATCCA 
GCAGTGGAAA CAGTACCCAT TTCTGTTTAT GATTCACTAA ATTTCGAGTT GAAACAGTTC 
GAACAGGAAG TTTTTAAGTC AAATAAAAGA TTTTCCAGAC TAAAGCAAGT TTTCAATAAT 
AAATCTTTGG AGTTTATTGA CGTTGTAAAC TCATTATTAG GTTTTAAATT GGAATTTCAA 
CAAGATAGTC GTGTGAAAAT ATTTTCCTGT TTCAAACCTG AAAAATATTT GATTGCTGAT 
CTAAATGAAA ACACTTTGAA ATCTAACCTT GATGCAGATA TAGAAGGATG GGATGATCTC 
ATGAATCTAT GGGTGGAAGA TAGAGGTCAA CTTCCGTGCT TTTTGGCAAC AATAACATTG 
CGTCTGTGGG AACAGCGACA AGCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MAD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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