PRP43 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP43
Entrez Gene ID	852757
Full Name	DEAH-box ATP-dependent RNA helicase PRP43
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180985.1	NP_011395.1	DEAH-box ATP-dependent RNA helicase PRP43

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dhx15	NP_031865.2
Saccharomyces cerevisiae (baker's yeast)	PRP43	NP_011395.1
Schizosaccharomyces pombe (fission yeast)	prp43	NP_595937.1
Oryza sativa (rice)	Os03g0314100	NP_001049931.1
Rattus norvegicus (Norway rat)	Dhx15	NP_001178526.1
Gallus gallus (chicken)	DHX15	NP_001026330.1
Danio rerio (zebrafish)	dhx15	NP_001108613.1
Kluyveromyces lactis	KLLA0B00561g	XP_451555.1
Magnaporthe oryzae (rice blast fungus)	MGG_03893	XP_003719983.1
Xenopus tropicalis (tropical clawed frog)	dhx15	NP_001135571.1
	DHX15	XP_001106067.1
Bos taurus (cattle)	DHX15	NP_001091487.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011149	XP_309498.4
Caenorhabditis elegans (roundworm)	F56D2.6	NP_741147.1
Homo sapiens (human)	DHX15	NP_001349.2
Drosophila melanogaster (fruit fly)	CG11107	NP_610269.1
Arabidopsis thaliana (thale cress)	AT3G62310	NP_191790.1
Pan troglodytes (chimpanzee)	DHX15	XP_001164599.1
Canis lupus familiaris (dog)	DHX15	XP_851535.1
Eremothecium gossypii	AGOS_AAR180C	NP_982723.1
Neurospora crassa	NCU01612	XP_956523.2
Arabidopsis thaliana (thale cress)	AT2G47250	NP_182247.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows the crystal structure of Prp43p bound to single-stranded poly-uracil RNA and reveals differences with viral ATPases in the RecA1 domain but with striking similarities with viral ATPases in the RecA2 domain of the catalytic core, including both a prominent beta-hairpin that bookends the 5' end of the RNA stack and a dynamic motif Va that is implicated in mediating translocation.
Title: Structure of the DEAH/RHA ATPase Prp43p bound to RNA implicates a pair of hairpins and motif Va in translocation along RNA.

Ntr1 and Ntr2 may act as 'doorkeepers' and both cofactors may inspect the RNP structure of spliceosomal complexes thereby targeting suboptimal spliceosomes for Prp43-mediated disassembly.
Title: Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2.

Using Prp43 F357A mutants or pyrimidine nucleotides, we show that the lack of stacking of the nucleotide base to the F-motif decouples the NTPase and helicase activities of Prp43. In contrast the R159A mutant (R-motif) showed reduced ATPase and helicase activities.
Title: Functional link between DEAH/RHA helicase Prp43 activation and ATP base binding.

These data indicate that the U2 snRNP-intron interaction is Prp43's major target.
Title: The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction.

Results show that Prp43 expression is regulated by Cmg1 which stimulates its RNA helicase activity.
Title: Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.

The following PRP43 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP43 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02128	NM_001180985.1 Latest version!	Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP43 (PRP43), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP43 ( NM_001180985.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011395.1 Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP43 (PRP43), partial mRNA.

Price & Availability↑

CloneID	OSi02128
Clone ID Related Accession (Same CDS sequence)	NM_001180985.1
Accession Version	NM_001180985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DEAH-box ATP-dependent RNA helicase PRP43
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTTCCA AAAGAAGATT CTCGTCCGAA CACCCGGATC CAGTTGAGAC CTCTATTCCT 
GAGCAGGCCG CCGAGATTGC GGAAGAATTA TCCAAACAGC ATCCTCTTCC ATCCGAAGAG 
CCACTTGTCC ATCATGATGC TGGTGAATTC AAGGGCTTGC AACGTCACCA TACTTCAGCA 
GAAGAGGCCC AAAAACTTGA AGATGGAAAA ATAAATCCAT TTACCGGTAG GGAGTTTACA 
CCAAAATATG TTGATATCCT GAAAATTAGA AGAGAATTGC CAGTACATGC CCAGAGAGAT 
GAGTTTTTAA AACTTTATCA AAATAACCAG ATTATGGTTT TTGTTGGTGA AACAGGTTCC 
GGTAAAACGA CACAAATTCC TCAATTCGTT TTATTCGATG AGATGCCACA CTTAGAGAAC 
ACTCAAGTTG CATGTACTCA ACCTCGTCGT GTCGCAGCAA TGTCTGTTGC ACAGAGAGTT 
GCTGAGGAAA TGGATGTCAA GTTGGGCGAA GAAGTTGGTT ATTCTATCAG ATTCGAAAAT 
AAAACTTCTA ATAAAACGAT ATTGAAGTAT ATGACTGATG GTATGTTGTT GAGAGAGGCA 
ATGGAAGACC ATGACTTGTC GCGTTATTCA TGTATCATTC TGGATGAAGC GCACGAACGT 
ACACTAGCTA CTGATATTTT GATGGGTCTT TTGAAGCAAG TAGTCAAGAG GAGACCTGAT 
TTGAAGATAA TTATTATGTC TGCTACTCTG GATGCAGAGA AGTTCCAACG CTACTTCAAC 
GATGCTCCAT TACTTGCCGT TCCGGGCAGA ACATATCCAG TTGAGCTATA CTATACACCG 
GAATTTCAAA GGGACTATTT AGATTCTGCC ATTCGTACTG TTTTGCAAAT TCATGCCACT 
GAAGAGGCTG GCGATATTTT GTTATTTTTA ACTGGTGAAG ATGAAATTGA AGACGCTGTC 
AGAAAGATTT CCTTAGAAGG TGATCAATTG GTTAGAGAAG AGGGATGCGG ACCTTTATCC 
GTATACCCAC TGTATGGTTC ATTACCACCG CATCAACAAC AAAGAATATT CGAGCCTGCT 
CCAGAGTCAC ATAATGGTAG ACCAGGCAGG AAAGTTGTTA TCTCCACGAA CATCGCTGAA 
ACTTCTTTAA CAATTGATGG TATTGTCTAC GTCGTTGACC CAGGATTTTC AAAGCAAAAA 
GTTTACAATC CAAGAATTAG AGTTGAGTCT TTACTTGTTT CCCCTATCTC CAAGGCTTCT 
GCCCAACAAA GAGCTGGTCG TGCTGGTCGT ACAAGGCCTG GTAAATGTTT CAGATTATAC 
ACTGAAGAGG CATTCCAAAA GGAACTGATA GAGCAAAGTT ACCCAGAAAT TTTACGTTCC 
AATTTATCCT CCACCGTTCT AGAACTAAAA AAATTAGGTA TTGATGACTT GGTCCATTTC 
GATTTTATGG ATCCACCGGC TCCGGAAACA ATGATGAGAG CCCTTGAAGA ACTAAATTAT 
TTGGCATGTC TGGACGACGA AGGTAACTTA ACACCCCTAG GTAGATTGGC ATCCCAGTTT 
CCCTTGGATC CTATGCTAGC GGTGATGTTA ATTGGGTCCT TTGAGTTCCA ATGTTCTCAA 
GAAATACTAA CTATAGTGGC CATGCTATCG GTTCCAAACG TGTTTATTCG CCCAACAAAG 
GACAAGAAGC GAGCAGATGA TGCCAAGAAT ATCTTTGCTC ATCCAGACGG TGATCATATC 
ACCTTACTAA ATGTATATCA TGCGTTCAAA TCGGATGAAG CTTATGAATA TGGTATCCAT 
AAGTGGTGCC GTGACCACTA TCTAAATTAC AGGTCCCTTT CCGCTGCTGA TAATATCCGC 
TCCCAATTAG AAAGATTAAT GAACCGTTAC AACCTAGAAT TAAACACTAC AGACTATGAA 
AGCCCTAAAT ACTTTGACAA CATCAGAAAG GCTCTTGCGT CTGGGTTTTT CATGCAAGTA 
GCAAAAAAAA GATCGGGAGC TAAAGGATAC ATCACAGTTA AAGATAACCA AGATGTTCTG 
ATCCATCCTA GTACGGTTCT TGGCCACGAC GCAGAATGGG TCATTTACAA TGAATTTGTG 
CTCACATCGA AGAACTACAT AAGAACTGTG ACCTCGGTCA GGCCCGAATG GCTGATTGAA 
ATAGCGCCTG CATACTACGA CTTAAGCAAT TTCCAAAAAG GTGATGTCAA ATTATCTTTG 
GAAAGGATCA AGGAAAAAGT TGATAGATTA AACGAGTTGA AACAAGGTAA AAACAAAAAG 
AAGAGTAAGC ACTCCAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011395.1
CDS	1..2304
Translation	MGSKRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVHHDAGEFKGLQRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEFLKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRVAEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLSRYSCIILDEAHERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAEKFQRYFNDAPLLAVPGRTYPVELYYTPEFQRDYLDSAIRTVLQIHATEEAGDILLFLTGEDEIEDAVRKISLEGDQLVREEGCGPLSVYPLYGSLPPHQQQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELIEQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDEGNLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRADDAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRDHYLNYRSLSAADNIRSQLERLMNRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGAKGYITVKDNQDVLIHPSTVLGHDAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSLERIKEKVDRLNELKQGKNKKKSKHSKK

Target ORF information:

RefSeq Version	NM_001180985.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DEAH-box ATP-dependent RNA helicase PRP43 (PRP43), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180985.1

ORF Insert Sequence:

ATGGGTTCCA AAAGAAGATT CTCGTCCGAA CACCCGGATC CAGTTGAGAC CTCTATTCCT 
GAGCAGGCCG CCGAGATTGC GGAAGAATTA TCCAAACAGC ATCCTCTTCC ATCCGAAGAG 
CCACTTGTCC ATCATGATGC TGGTGAATTC AAGGGCTTGC AACGTCACCA TACTTCAGCA 
GAAGAGGCCC AAAAACTTGA AGATGGAAAA ATAAATCCAT TTACCGGTAG GGAGTTTACA 
CCAAAATATG TTGATATCCT GAAAATTAGA AGAGAATTGC CAGTACATGC CCAGAGAGAT 
GAGTTTTTAA AACTTTATCA AAATAACCAG ATTATGGTTT TTGTTGGTGA AACAGGTTCC 
GGTAAAACGA CACAAATTCC TCAATTCGTT TTATTCGATG AGATGCCACA CTTAGAGAAC 
ACTCAAGTTG CATGTACTCA ACCTCGTCGT GTCGCAGCAA TGTCTGTTGC ACAGAGAGTT 
GCTGAGGAAA TGGATGTCAA GTTGGGCGAA GAAGTTGGTT ATTCTATCAG ATTCGAAAAT 
AAAACTTCTA ATAAAACGAT ATTGAAGTAT ATGACTGATG GTATGTTGTT GAGAGAGGCA 
ATGGAAGACC ATGACTTGTC GCGTTATTCA TGTATCATTC TGGATGAAGC GCACGAACGT 
ACACTAGCTA CTGATATTTT GATGGGTCTT TTGAAGCAAG TAGTCAAGAG GAGACCTGAT 
TTGAAGATAA TTATTATGTC TGCTACTCTG GATGCAGAGA AGTTCCAACG CTACTTCAAC 
GATGCTCCAT TACTTGCCGT TCCGGGCAGA ACATATCCAG TTGAGCTATA CTATACACCG 
GAATTTCAAA GGGACTATTT AGATTCTGCC ATTCGTACTG TTTTGCAAAT TCATGCCACT 
GAAGAGGCTG GCGATATTTT GTTATTTTTA ACTGGTGAAG ATGAAATTGA AGACGCTGTC 
AGAAAGATTT CCTTAGAAGG TGATCAATTG GTTAGAGAAG AGGGATGCGG ACCTTTATCC 
GTATACCCAC TGTATGGTTC ATTACCACCG CATCAACAAC AAAGAATATT CGAGCCTGCT 
CCAGAGTCAC ATAATGGTAG ACCAGGCAGG AAAGTTGTTA TCTCCACGAA CATCGCTGAA 
ACTTCTTTAA CAATTGATGG TATTGTCTAC GTCGTTGACC CAGGATTTTC AAAGCAAAAA 
GTTTACAATC CAAGAATTAG AGTTGAGTCT TTACTTGTTT CCCCTATCTC CAAGGCTTCT 
GCCCAACAAA GAGCTGGTCG TGCTGGTCGT ACAAGGCCTG GTAAATGTTT CAGATTATAC 
ACTGAAGAGG CATTCCAAAA GGAACTGATA GAGCAAAGTT ACCCAGAAAT TTTACGTTCC 
AATTTATCCT CCACCGTTCT AGAACTAAAA AAATTAGGTA TTGATGACTT GGTCCATTTC 
GATTTTATGG ATCCACCGGC TCCGGAAACA ATGATGAGAG CCCTTGAAGA ACTAAATTAT 
TTGGCATGTC TGGACGACGA AGGTAACTTA ACACCCCTAG GTAGATTGGC ATCCCAGTTT 
CCCTTGGATC CTATGCTAGC GGTGATGTTA ATTGGGTCCT TTGAGTTCCA ATGTTCTCAA 
GAAATACTAA CTATAGTGGC CATGCTATCG GTTCCAAACG TGTTTATTCG CCCAACAAAG 
GACAAGAAGC GAGCAGATGA TGCCAAGAAT ATCTTTGCTC ATCCAGACGG TGATCATATC 
ACCTTACTAA ATGTATATCA TGCGTTCAAA TCGGATGAAG CTTATGAATA TGGTATCCAT 
AAGTGGTGCC GTGACCACTA TCTAAATTAC AGGTCCCTTT CCGCTGCTGA TAATATCCGC 
TCCCAATTAG AAAGATTAAT GAACCGTTAC AACCTAGAAT TAAACACTAC AGACTATGAA 
AGCCCTAAAT ACTTTGACAA CATCAGAAAG GCTCTTGCGT CTGGGTTTTT CATGCAAGTA 
GCAAAAAAAA GATCGGGAGC TAAAGGATAC ATCACAGTTA AAGATAACCA AGATGTTCTG 
ATCCATCCTA GTACGGTTCT TGGCCACGAC GCAGAATGGG TCATTTACAA TGAATTTGTG 
CTCACATCGA AGAACTACAT AAGAACTGTG ACCTCGGTCA GGCCCGAATG GCTGATTGAA 
ATAGCGCCTG CATACTACGA CTTAAGCAAT TTCCAAAAAG GTGATGTCAA ATTATCTTTG 
GAAAGGATCA AGGAAAAAGT TGATAGATTA AACGAGTTGA AACAAGGTAA AAACAAAAAG 
AAGAGTAAGC ACTCCAAGAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

PRP43 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報