ATG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ATG1
Entrez Gene ID	852695
Full Name	serine/threonine protein kinase ATG1
Synonyms	APG1,AUT3,CVT10
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181045.1	NP_011335.1	serine/threonine protein kinase ATG1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C17160g	XP_452967.1
Magnaporthe oryzae (rice blast fungus)	MGG_06393	XP_003717178.1
Neurospora crassa	NCU00188	XP_956411.1
Saccharomyces cerevisiae (baker's yeast)	ATG1	NP_011335.1
Eremothecium gossypii	AGOS_ACL054W	NP_983350.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that phosphorylation by Atg1 inhibits Atg4 at autophagosome formation sites.
Title: Atg4 proteolytic activity can be inhibited by Atg1 phosphorylation.

The article shows how selective autophagy is tightly regulated in space and time to prevent aberrant Atg1 kinase activation and autophagy induction.
Title: Two Independent Pathways within Selective Autophagy Converge to Activate Atg1 Kinase at the Vacuole.

In vitro studies show that Atg1 kinase is activated by selective autophagy targets in yeast requiring the Atg11 scaffold.
Title: Receptor-Bound Targets of Selective Autophagy Use a Scaffold Protein to Activate the Atg1 Kinase.

Atg1 complex provide insights into assembly and regulatory mechanisms
Title: Molecular interactions of the Saccharomyces cerevisiae Atg1 complex provide insights into assembly and regulatory mechanisms.

These results lead to a model for autophagy initiation in which Atg1 and Atg13 are tightly associated with one another and assemble transiently into the pentameric Atg1 complex during starvation.
Title: Assembly and dynamics of the autophagy-initiating Atg1 complex.

The following ATG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02073	NM_001181045.1 Latest version!	Saccharomyces cerevisiae S288C serine/threonine protein kinase ATG1 (ATG1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATG1 ( NM_001181045.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011335.1 Saccharomyces cerevisiae S288C serine/threonine protein kinase ATG1 (ATG1), partial mRNA.

Price & Availability↑

CloneID	OSi02073
Clone ID Related Accession (Same CDS sequence)	NM_001181045.1
Accession Version	NM_001181045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	serine/threonine protein kinase ATG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGAGACA TTAAAAATAA AGATCACACA ACCTCTGTGA ACCATAATCT AATGGCAAGT 
GCAGGAAATT ACACCGCTGA GAAAGAAATC GGAAAGGGTT CGTTTGCCAC TGTATATAGA 
GGGCATCTGA CATCCGACAA ATCTCAGCAT GTAGCCATAA AGGAAGTATC AAGGGCGAAA 
TTAAAAAATA AGAAATTACT AGAGAATTTG GAAATAGAAA TCGCTATCTT GAAGAAAATC 
AAGCATCCTC ATATCGTCGG ACTTATTGAC TGTGAACGAA CATCAACAGA TTTTTATTTG 
ATCATGGAGT ACTGTGCTCT TGGGGACCTA ACATTTCTGT TGAAAAGGCG TAAAGAATTG 
ATGGAGAATC ATCCTCTACT AAGGACCGTA TTTGAAAAAT ACCCTCCACC GAGTGAGAAC 
CATAACGGCC TGCATAGAGC GTTTGTCTTG AGTTATTTAC AGCAGTTAGC GTCCGCTTTG 
AAATTTTTGA GGTCTAAAAA CTTGGTTCAT AGAGACATCA AGCCTCAAAA CTTACTATTA 
TCTACACCCC TAATTGGATA TCATGATTCA AAAAGCTTCC ATGAACTTGG ATTTGTTGGG 
ATCTACAACT TACCCATTTT AAAGATAGCA GATTTCGGGT TTGCAAGATT TTTGCCAAAC 
ACGTCATTAG CAGAAACTCT TTGTGGCTCA CCATTATATA TGGCACCAGA AATTTTGAAT 
TATCAAAAAT ATAACGCTAA AGCAGATCTG TGGTCTGTCG GTACAGTGGT ATTCGAAATG 
TGCTGTGGCA CCCCACCGTT TAGAGCTTCC AATCATTTGG AGTTATTCAA GAAAATTAAA 
AGAGCAAACG ATGTCATAAC GTTTCCTTCA TATTGCAATA TTGAACCAGA GTTAAAAGAG 
TTGATATGTA GTTTATTGAC ATTTGATCCA GCCCAAAGAA TAGGATTTGA GGAGTTTTTT 
GCTAACAAGG TAGTCAACGA AGACTTGTCT TCTTATGAAT TGGAAGATGA TTTACCTGAG 
TTAGAATCCA AATCAAAAGG TATTGTAGAA AGTAATATGT TCGTTTCTGA GTATTTATCT 
AAACAGCCAA AGAGTCCGAA CAGTAATCTT GCAGGTCATC AATCAATGGC AGATAATCCC 
GCGGAGCTCA GTGATGCCCT CAAGAACAGC AATATATTAA CTGCCCCAGC CGTTAAAACA 
GACCATACAC AAGCCGTAGA TAAAAAGGCT TCAAATAATA AATACCATAA TAGCCTAGTT 
TCAGATAGAA GCTTTGAAAG AGAATATGTG GTGGTAGAGA AGAAATCGGT TGAAGTTAAT 
TCATTGGCAG ACGAGGTTGC TCAAGCAGGA TTCAATCCAA ATCCTATTAA GCACCCAACT 
TCAACTCAAA ATCAGAACGT CTTATTGAAT GAGCAATTCT CTCCAAACAA TCAACAGTAT 
TTTCAAAATC AAGGAGAAAA TCCGAGGTTA CTGAGGGCCA CATCATCTTC CAGTGGAGGT 
AGTGATGGGT CTAGGCGACC ATCTTTGGTG GATAGACGCT TGTCTATATC CTCGCTGAAT 
CCATCTAATG CATTATCAAG AGCCCTCGGT ATTGCATCAA CGAGATTGTT TGGTGGTGCA 
AATCAACAGC AGCAACAGCA ACAAATCACA TCTTCCCCAC CGTACAGTCA AACTTTGTTG 
AATTCCCAAC TTTTTCATGA ACTTACTGAG AATATAATAT TAAGAATAGA TCACCTACAG 
CATCCAGAGA CACTGAAATT AGATAATACT AATATCGTTA GTATTTTGGA ATCTCTGGCC 
GCAAAGGCAT TTGTTGTTTA CTCTTATGCA GAAGTGAAAT TTTCTCAAAT TGTTCCATTA 
TCAACAACAT TAAAAGGCAT GGCTAACTTT GAGAACAGGC GCAGTATGGA TAGTAATGCT 
ATTGCAGAAG AACAAGACTC AGACGATGCA GAAGAAGAGG ATGAAACGTT GAAAAAGTAC 
AAGGAAGATT GCTTATCCAC GAAAACTTTT GGAAAGGGTA GAACTTTATC TGCCACATCT 
CAGTTGAGTG CAACTTTCAA TAAACTACCA CGTTCGGAAA TGATCCTTCT ATGTAATGAG 
GCCATTGTCT TATATATGAA GGCATTATCC ATTTTATCAA AATCTATGCA GGTAACGTCC 
AACTGGTGGT ATGAATCTCA AGAAAAATCA TGTTCTCTAA GAGTTAACGT GTTGGTACAG 
TGGCTAAGGG AAAAATTTAA TGAATGTTTA GAAAAAGCTG ATTTCCTGAG ATTAAAAATT 
AACGACTTGA GATTCAAGCA TGCTTCTGAG GTAGCTGAAA ATCAAACTTT GGAAGAAAAA 
GGTAGTTCGG AAGAGCCAGT ATATTTAGAA AAGCTATTAT ATGATCGTGC ATTAGAAATA 
TCTAAGATGG CCGCACATAT GGAATTAAAA GGAGAAAATT TGTACAACTG TGAATTAGCT 
TATGCAACTT CACTTTGGAT GTTAGAAACT TCTCTGGACG ATGATGATTT TACGAATGCC 
TATGGTGACT ACCCTTTTAA AACTAACATA CATCTAAAAA GTAACGACGT TGAAGATAAA 
GAGAAGTATC ATAGTGTGTT GGATGAAAAT GACAGAATAA TCATAAGAAA GTATATTGAT 
AGTATTGCAA ACAGGTTGAA AATATTGAGG CAGAAGATGA ACCACCAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011335.1
CDS	1..2694
Translation	MGDIKNKDHTTSVNHNLMASAGNYTAEKEIGKGSFATVYRGHLTSDKSQHVAIKEVSRAKLKNKKLLENLEIEIAILKKIKHPHIVGLIDCERTSTDFYLIMEYCALGDLTFLLKRRKELMENHPLLRTVFEKYPPPSENHNGLHRAFVLSYLQQLASALKFLRSKNLVHRDIKPQNLLLSTPLIGYHDSKSFHELGFVGIYNLPILKIADFGFARFLPNTSLAETLCGSPLYMAPEILNYQKYNAKADLWSVGTVVFEMCCGTPPFRASNHLELFKKIKRANDVITFPSYCNIEPELKELICSLLTFDPAQRIGFEEFFANKVVNEDLSSYELEDDLPELESKSKGIVESNMFVSEYLSKQPKSPNSNLAGHQSMADNPAELSDALKNSNILTAPAVKTDHTQAVDKKASNNKYHNSLVSDRSFEREYVVVEKKSVEVNSLADEVAQAGFNPNPIKHPTSTQNQNVLLNEQFSPNNQQYFQNQGENPRLLRATSSSSGGSDGSRRPSLVDRRLSISSLNPSNALSRALGIASTRLFGGANQQQQQQQITSSPPYSQTLLNSQLFHELTENIILRIDHLQHPETLKLDNTNIVSILESLAAKAFVVYSYAEVKFSQIVPLSTTLKGMANFENRRSMDSNAIAEEQDSDDAEEEDETLKKYKEDCLSTKTFGKGRTLSATSQLSATFNKLPRSEMILLCNEAIVLYMKALSILSKSMQVTSNWWYESQEKSCSLRVNVLVQWLREKFNECLEKADFLRLKINDLRFKHASEVAENQTLEEKGSSEEPVYLEKLLYDRALEISKMAAHMELKGENLYNCELAYATSLWMLETSLDDDDFTNAYGDYPFKTNIHLKSNDVEDKEKYHSVLDENDRIIIRKYIDSIANRLKILRQKMNHQN

Target ORF information:

RefSeq Version	NM_001181045.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C serine/threonine protein kinase ATG1 (ATG1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181045.1

ORF Insert Sequence:

ATGGGAGACA TTAAAAATAA AGATCACACA ACCTCTGTGA ACCATAATCT AATGGCAAGT 
GCAGGAAATT ACACCGCTGA GAAAGAAATC GGAAAGGGTT CGTTTGCCAC TGTATATAGA 
GGGCATCTGA CATCCGACAA ATCTCAGCAT GTAGCCATAA AGGAAGTATC AAGGGCGAAA 
TTAAAAAATA AGAAATTACT AGAGAATTTG GAAATAGAAA TCGCTATCTT GAAGAAAATC 
AAGCATCCTC ATATCGTCGG ACTTATTGAC TGTGAACGAA CATCAACAGA TTTTTATTTG 
ATCATGGAGT ACTGTGCTCT TGGGGACCTA ACATTTCTGT TGAAAAGGCG TAAAGAATTG 
ATGGAGAATC ATCCTCTACT AAGGACCGTA TTTGAAAAAT ACCCTCCACC GAGTGAGAAC 
CATAACGGCC TGCATAGAGC GTTTGTCTTG AGTTATTTAC AGCAGTTAGC GTCCGCTTTG 
AAATTTTTGA GGTCTAAAAA CTTGGTTCAT AGAGACATCA AGCCTCAAAA CTTACTATTA 
TCTACACCCC TAATTGGATA TCATGATTCA AAAAGCTTCC ATGAACTTGG ATTTGTTGGG 
ATCTACAACT TACCCATTTT AAAGATAGCA GATTTCGGGT TTGCAAGATT TTTGCCAAAC 
ACGTCATTAG CAGAAACTCT TTGTGGCTCA CCATTATATA TGGCACCAGA AATTTTGAAT 
TATCAAAAAT ATAACGCTAA AGCAGATCTG TGGTCTGTCG GTACAGTGGT ATTCGAAATG 
TGCTGTGGCA CCCCACCGTT TAGAGCTTCC AATCATTTGG AGTTATTCAA GAAAATTAAA 
AGAGCAAACG ATGTCATAAC GTTTCCTTCA TATTGCAATA TTGAACCAGA GTTAAAAGAG 
TTGATATGTA GTTTATTGAC ATTTGATCCA GCCCAAAGAA TAGGATTTGA GGAGTTTTTT 
GCTAACAAGG TAGTCAACGA AGACTTGTCT TCTTATGAAT TGGAAGATGA TTTACCTGAG 
TTAGAATCCA AATCAAAAGG TATTGTAGAA AGTAATATGT TCGTTTCTGA GTATTTATCT 
AAACAGCCAA AGAGTCCGAA CAGTAATCTT GCAGGTCATC AATCAATGGC AGATAATCCC 
GCGGAGCTCA GTGATGCCCT CAAGAACAGC AATATATTAA CTGCCCCAGC CGTTAAAACA 
GACCATACAC AAGCCGTAGA TAAAAAGGCT TCAAATAATA AATACCATAA TAGCCTAGTT 
TCAGATAGAA GCTTTGAAAG AGAATATGTG GTGGTAGAGA AGAAATCGGT TGAAGTTAAT 
TCATTGGCAG ACGAGGTTGC TCAAGCAGGA TTCAATCCAA ATCCTATTAA GCACCCAACT 
TCAACTCAAA ATCAGAACGT CTTATTGAAT GAGCAATTCT CTCCAAACAA TCAACAGTAT 
TTTCAAAATC AAGGAGAAAA TCCGAGGTTA CTGAGGGCCA CATCATCTTC CAGTGGAGGT 
AGTGATGGGT CTAGGCGACC ATCTTTGGTG GATAGACGCT TGTCTATATC CTCGCTGAAT 
CCATCTAATG CATTATCAAG AGCCCTCGGT ATTGCATCAA CGAGATTGTT TGGTGGTGCA 
AATCAACAGC AGCAACAGCA ACAAATCACA TCTTCCCCAC CGTACAGTCA AACTTTGTTG 
AATTCCCAAC TTTTTCATGA ACTTACTGAG AATATAATAT TAAGAATAGA TCACCTACAG 
CATCCAGAGA CACTGAAATT AGATAATACT AATATCGTTA GTATTTTGGA ATCTCTGGCC 
GCAAAGGCAT TTGTTGTTTA CTCTTATGCA GAAGTGAAAT TTTCTCAAAT TGTTCCATTA 
TCAACAACAT TAAAAGGCAT GGCTAACTTT GAGAACAGGC GCAGTATGGA TAGTAATGCT 
ATTGCAGAAG AACAAGACTC AGACGATGCA GAAGAAGAGG ATGAAACGTT GAAAAAGTAC 
AAGGAAGATT GCTTATCCAC GAAAACTTTT GGAAAGGGTA GAACTTTATC TGCCACATCT 
CAGTTGAGTG CAACTTTCAA TAAACTACCA CGTTCGGAAA TGATCCTTCT ATGTAATGAG 
GCCATTGTCT TATATATGAA GGCATTATCC ATTTTATCAA AATCTATGCA GGTAACGTCC 
AACTGGTGGT ATGAATCTCA AGAAAAATCA TGTTCTCTAA GAGTTAACGT GTTGGTACAG 
TGGCTAAGGG AAAAATTTAA TGAATGTTTA GAAAAAGCTG ATTTCCTGAG ATTAAAAATT 
AACGACTTGA GATTCAAGCA TGCTTCTGAG GTAGCTGAAA ATCAAACTTT GGAAGAAAAA 
GGTAGTTCGG AAGAGCCAGT ATATTTAGAA AAGCTATTAT ATGATCGTGC ATTAGAAATA 
TCTAAGATGG CCGCACATAT GGAATTAAAA GGAGAAAATT TGTACAACTG TGAATTAGCT 
TATGCAACTT CACTTTGGAT GTTAGAAACT TCTCTGGACG ATGATGATTT TACGAATGCC 
TATGGTGACT ACCCTTTTAA AACTAACATA CATCTAAAAA GTAACGACGT TGAAGATAAA 
GAGAAGTATC ATAGTGTGTT GGATGAAAAT GACAGAATAA TCATAAGAAA GTATATTGAT 
AGTATTGCAA ACAGGTTGAA AATATTGAGG CAGAAGATGA ACCACCAAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

ATG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報