ORF » Species Summary » Saccharomyces cerevisiae S288C » PCA1 cDNA ORF clone

PCA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following PCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OSi01989 NM_001178643.2
Latest version!		 Saccharomyces cerevisiae S288C cation-transporting P-type ATPase PCA1 (PCA1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PCA1 ( NM_001178643.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009854.2 Saccharomyces cerevisiae S288C cation-transporting P-type ATPase PCA1 (PCA1), partial mRNA.
    Price & Availability↑
    CloneID OSi01989
    Clone ID Related Accession (Same CDS sequence) NM_001178643.2
    Accession Version NM_001178643.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product cation-transporting P-type ATPase PCA1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178643.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGCCGG AAAAACTCTT CTCCGGTTTA GGTACCAGCG ATGGCGAGTA TGGAGTGGTA 
    AATAGCGAGA ACATATCAAT AGATGCTATG CAAGATAATA GAGGCGAGTG TCATCGTAGA 
    TCCATAGAAA TGCATGCTAA TGACAATCTG GGATTGGTCT CCCAGCGAGA CTGTACCAAT 
    CGGCCCAAAA TTACTCCTCA GGAATGTTTG AGTGAGACTG AGCAGATATG CCATCATGGC 
    GAGAATAGGA CTAAGGCTGG GTTAGATGTA GACGATGCTG AAACCGGTGG TGATCACACC 
    AATGAATCCC GTGTAGATGA ATGTTGTGCT GAGAAAGTGA ACGACACTGA GACTGGCTTG 
    GATGTGGACA GCTGTTGCGG CGATGCTCAA ACAGGTGGTG ACCACACCAA TGAATCCTGT 
    GTTGATGGAT GCTGCGTTAG AGATTCTTCA GTGATGGTCG AGGAAGTTAC AGGCAGCTGC 
    GAAGCTGTAT CTTCTAAAGA ACAATTGCTG ACCAGCTTTG AAGTTGTTCC AAGCAAATCT 
    GAAGGTCTGC AATCTATTCA TGATATTAGA GAGACAACTC GCTGCAATAC CAATTCCAAC 
    CAACACACAG GGAAGGGTAG GCTTTGCATC GAATCAAGTG ACTCTACACT CAAGAAAAGA 
    AGTTGTAAAG TTAGCAGACA GAAAATTGAG GTTTCCAGTA AGCCTGAGTG CTGCAACATT 
    TCATGTGTTG AGCGGATTGC ATCTCGTAGC TGTGAAAAGA GGACTTTTAA AGGTAGCACC 
    AATGTTGGAA TTTCTGGGAG TAGCTCTACC GACAGTTTAA GCGAGAAGTT CTTCAGCGAA 
    CAGTACTCTA GAATGTACAA TCGTTACTCC TCAATTTTGA AAAATTTAGG TTGTATCTGT 
    AACTATTTGC GTACTTTGGG AAAAGAATCT TGTTGTCTCC CAAAGGTGCG TTTTTGTAGC 
    GGGGAGGGTG CTTCTAAAAA GACAAAATAC TCCTACCGCA ACAGTTCTGG ATGTTTAACA 
    AAGAAAAAGA CCCATGGAGA CAAAGAAAGG TTGAGCAATG ACAATGGCCA TGCTGATTTT 
    GTTTGTTCTA AAAGTTGTTG CACTAAAATG AAGGATTGTG CTGTTACCTC AACTATTTCT 
    GGACACTCTT CGAGTGAAAT TTCAAGAATC GTATCAATGG AACCAATTGA AAATCATCTT 
    AATCTTGAGG CAGGATCTAC CGGTACCGAG CACATTGTTC TTAGTGTTTC AGGAATGTCC 
    TGTACTGGTT GTGAATCAAA ACTTAAGAAA TCATTTGGCG CGCTCAAATG TGTTCACGGT 
    TTGAAGACCA GTTTAATATT ATCGCAAGCT GAATTTAATC TGGATCTTGC TCAGGGATCT 
    GTCAAGGATG TTATCAAGCA CTTGAGCAAA ACTACTGAAT TCAAGTACGA ACAGATTTCA 
    AATCATGGTT CAACTATAGA TGTTGTTGTT CCTTATGCAG CAAAAGATTT TATTAATGAG 
    GAATGGCCAC AAGGTGTCAC AGAGCTGAAA ATTGTTGAGA GAAATATCAT TCGTATTTAC 
    TTTGACCCAA AGGTTATAGG TGCCAGAGAT CTTGTCAATG AAGGATGGAG CGTGCCTGTT 
    AGTATTGCTC CATTCAGTTG TCATCCAACC ATTGAAGTGG GAAGGAAGCA TTTAGTTCGT 
    GTAGGCTGCA CGACCGCTTT ATCCATAATA TTGACGATCC CGATTCTCGT GATGGCTTGG 
    GCTCCACAGC TTCGTGAAAA AATTTCCACT ATCTCCGCGT CAATGGTGCT AGCAACTATT 
    ATTCAATTTG TTATTGCAGG ACCGTTTTAC TTAAATGCCT TGAAGAGTTT GATATTTTCC 
    AGGCTCATTG AAATGGATCT ACTAATAGTT TTAAGCACTA GCGCTGCCTA CATATTTTCG 
    ATTGTATCAT TTGGATATTT CGTTGTTGGA CGCCCGTTAT CTACGGAGCA GTTTTTTGAA 
    ACCAGCTCTT TGCTTGTAAC TCTCATCATG GTTGGTCGCT TTGTGAGTGA ACTAGCTAGA 
    CATAGGGCTG TAAAGTCAAT ATCAGTACGT TCCTTACAGG CCTCTTCTGC TATTCTGGTG 
    GATAAAACTG GTAAAGAGAC GGAAATTAAT ATTAGACTTC TCCAATATGG CGATATTTTT 
    AAGGTTTTAC CTGATTCAAG AATCCCAACT GATGGAACAG TCATTTCTGG GTCTTCTGAA 
    GTCGACGAAG CGTTAATCAC AGGTGAATCT ATGCCAGTCC CGAAAAAGTG TCAATCAATT 
    GTTGTTGCAG GATCGGTGAA TGGCACTGGT ACTTTGTTTG TGAAGCTGAG CAAGCTTCCA 
    GGGAACAATA CTATTAGTAC CATAGCCACG ATGGTTGATG AAGCAAAATT AACTAAGCCA 
    AAGATCCAAA ATATCGCTGA TAAAATTGCA AGTTATTTCG TGCCAACTAT CATTGGAATC 
    ACTGTCGTCA CCTTTTGCGT TTGGATAGCG GTTGGAATCC GTGTGGAAAA GCAATCCCGC 
    TCCGATGCCG TAATTCAGGC CATTATATAT GCCATTACGG TACTTATCGT TTCGTGTCCC 
    TGCGTGATTG GACTTGCCGT TCCTATCGTA TTTGTTATTG CAAGTGGCGT CGCTGCGAAA 
    AGAGGGGTAA TCTTCAAATC AGCAGAGAGT ATAGAAGTTG CTCACAACAC TTCGCATGTT 
    GTCTTCGATA AAACGGGTAC CCTAACTGAA GGAAAACTTA CTGTAGTACA TGAAACTGTT 
    AGGGGTGATC GTCATAATTC TCAATCTTTG TTGCTTGGAT TAACTGAAGG AATAAAACAC 
    CCAGTTTCCA TGGCAATAGC ATCCTATCTC AAAGAAAAAG GTGTTTCTGC TCAAAATGTT 
    TCTAATACAA AAGCTGTGAC TGGTAAGCGG GTAGAAGGAA CATCATACTC TGGTTTGAAG 
    TTACAAGGAG GGAACTGTCG TTGGCTTGGT CATAACAATG ATCCTGACGT TCGAAAAGCC 
    CTTGAACAAG GATATTCTGT ATTCTGTTTT AGTGTTAATG GTTCAGTCAC TGCAGTTTAT 
    GCATTAGAGG ACTCTTTACG GGCAGATGCT GTCTCCACTA TTAACTTGTT ACGCCAAAGA 
    GGGATTTCAC TACACATTTT ATCAGGGGAT GACGATGGAG CCGTTCGTTC TATGGCAGCC 
    CGTCTTGGAA TTGAAAGCTC CAATATTCGT TCTCACGCAA CGCCTGCAGA AAAGAGTGAA 
    TATATTAAGG ATATTGTCGA AGGAAGAAAT TGCGATAGTT CTTCGCAGTC AAAAAGACCG 
    GTTGTTGTTT TTTGCGGTGA CGGAACAAAC GACGCAATTG GTTTGACTCA AGCAACGATT 
    GGAGTTCATA TCAATGAGGG AAGTGAGGTT GCCAAGCTAG CTGCTGATGT AGTTATGTTA 
    AAGCCAAAGC TCAACAATAT TTTGACTATG ATAACTGTAA GTCAAAAGGC CATGTTTAGG 
    GTCAAATTGA ATTTCTTATG GAGTTTTACT TACAACTTAT TTGCTATCCT TTTGGCGGCT 
    GGTGCCTTTG TTGACTTTCA TATTCCACCA GAGTATGCGG GTTTAGGGGA ACTAGTTAGT 
    ATCCTCCCTG TAATTTTTGT AGCTATACTT CTGCGTTATG CAAAGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009854.2
    CDS1..3651
    Translation

    Target ORF information:

    RefSeq Version NM_001178643.2
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C cation-transporting P-type ATPase PCA1 (PCA1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178643.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGCCGG AAAAACTCTT CTCCGGTTTA GGTACCAGCG ATGGCGAGTA TGGAGTGGTA 
    AATAGCGAGA ACATATCAAT AGATGCTATG CAAGATAATA GAGGCGAGTG TCATCGTAGA 
    TCCATAGAAA TGCATGCTAA TGACAATCTG GGATTGGTCT CCCAGCGAGA CTGTACCAAT 
    CGGCCCAAAA TTACTCCTCA GGAATGTTTG AGTGAGACTG AGCAGATATG CCATCATGGC 
    GAGAATAGGA CTAAGGCTGG GTTAGATGTA GACGATGCTG AAACCGGTGG TGATCACACC 
    AATGAATCCC GTGTAGATGA ATGTTGTGCT GAGAAAGTGA ACGACACTGA GACTGGCTTG 
    GATGTGGACA GCTGTTGCGG CGATGCTCAA ACAGGTGGTG ACCACACCAA TGAATCCTGT 
    GTTGATGGAT GCTGCGTTAG AGATTCTTCA GTGATGGTCG AGGAAGTTAC AGGCAGCTGC 
    GAAGCTGTAT CTTCTAAAGA ACAATTGCTG ACCAGCTTTG AAGTTGTTCC AAGCAAATCT 
    GAAGGTCTGC AATCTATTCA TGATATTAGA GAGACAACTC GCTGCAATAC CAATTCCAAC 
    CAACACACAG GGAAGGGTAG GCTTTGCATC GAATCAAGTG ACTCTACACT CAAGAAAAGA 
    AGTTGTAAAG TTAGCAGACA GAAAATTGAG GTTTCCAGTA AGCCTGAGTG CTGCAACATT 
    TCATGTGTTG AGCGGATTGC ATCTCGTAGC TGTGAAAAGA GGACTTTTAA AGGTAGCACC 
    AATGTTGGAA TTTCTGGGAG TAGCTCTACC GACAGTTTAA GCGAGAAGTT CTTCAGCGAA 
    CAGTACTCTA GAATGTACAA TCGTTACTCC TCAATTTTGA AAAATTTAGG TTGTATCTGT 
    AACTATTTGC GTACTTTGGG AAAAGAATCT TGTTGTCTCC CAAAGGTGCG TTTTTGTAGC 
    GGGGAGGGTG CTTCTAAAAA GACAAAATAC TCCTACCGCA ACAGTTCTGG ATGTTTAACA 
    AAGAAAAAGA CCCATGGAGA CAAAGAAAGG TTGAGCAATG ACAATGGCCA TGCTGATTTT 
    GTTTGTTCTA AAAGTTGTTG CACTAAAATG AAGGATTGTG CTGTTACCTC AACTATTTCT 
    GGACACTCTT CGAGTGAAAT TTCAAGAATC GTATCAATGG AACCAATTGA AAATCATCTT 
    AATCTTGAGG CAGGATCTAC CGGTACCGAG CACATTGTTC TTAGTGTTTC AGGAATGTCC 
    TGTACTGGTT GTGAATCAAA ACTTAAGAAA TCATTTGGCG CGCTCAAATG TGTTCACGGT 
    TTGAAGACCA GTTTAATATT ATCGCAAGCT GAATTTAATC TGGATCTTGC TCAGGGATCT 
    GTCAAGGATG TTATCAAGCA CTTGAGCAAA ACTACTGAAT TCAAGTACGA ACAGATTTCA 
    AATCATGGTT CAACTATAGA TGTTGTTGTT CCTTATGCAG CAAAAGATTT TATTAATGAG 
    GAATGGCCAC AAGGTGTCAC AGAGCTGAAA ATTGTTGAGA GAAATATCAT TCGTATTTAC 
    TTTGACCCAA AGGTTATAGG TGCCAGAGAT CTTGTCAATG AAGGATGGAG CGTGCCTGTT 
    AGTATTGCTC CATTCAGTTG TCATCCAACC ATTGAAGTGG GAAGGAAGCA TTTAGTTCGT 
    GTAGGCTGCA CGACCGCTTT ATCCATAATA TTGACGATCC CGATTCTCGT GATGGCTTGG 
    GCTCCACAGC TTCGTGAAAA AATTTCCACT ATCTCCGCGT CAATGGTGCT AGCAACTATT 
    ATTCAATTTG TTATTGCAGG ACCGTTTTAC TTAAATGCCT TGAAGAGTTT GATATTTTCC 
    AGGCTCATTG AAATGGATCT ACTAATAGTT TTAAGCACTA GCGCTGCCTA CATATTTTCG 
    ATTGTATCAT TTGGATATTT CGTTGTTGGA CGCCCGTTAT CTACGGAGCA GTTTTTTGAA 
    ACCAGCTCTT TGCTTGTAAC TCTCATCATG GTTGGTCGCT TTGTGAGTGA ACTAGCTAGA 
    CATAGGGCTG TAAAGTCAAT ATCAGTACGT TCCTTACAGG CCTCTTCTGC TATTCTGGTG 
    GATAAAACTG GTAAAGAGAC GGAAATTAAT ATTAGACTTC TCCAATATGG CGATATTTTT 
    AAGGTTTTAC CTGATTCAAG AATCCCAACT GATGGAACAG TCATTTCTGG GTCTTCTGAA 
    GTCGACGAAG CGTTAATCAC AGGTGAATCT ATGCCAGTCC CGAAAAAGTG TCAATCAATT 
    GTTGTTGCAG GATCGGTGAA TGGCACTGGT ACTTTGTTTG TGAAGCTGAG CAAGCTTCCA 
    GGGAACAATA CTATTAGTAC CATAGCCACG ATGGTTGATG AAGCAAAATT AACTAAGCCA 
    AAGATCCAAA ATATCGCTGA TAAAATTGCA AGTTATTTCG TGCCAACTAT CATTGGAATC 
    ACTGTCGTCA CCTTTTGCGT TTGGATAGCG GTTGGAATCC GTGTGGAAAA GCAATCCCGC 
    TCCGATGCCG TAATTCAGGC CATTATATAT GCCATTACGG TACTTATCGT TTCGTGTCCC 
    TGCGTGATTG GACTTGCCGT TCCTATCGTA TTTGTTATTG CAAGTGGCGT CGCTGCGAAA 
    AGAGGGGTAA TCTTCAAATC AGCAGAGAGT ATAGAAGTTG CTCACAACAC TTCGCATGTT 
    GTCTTCGATA AAACGGGTAC CCTAACTGAA GGAAAACTTA CTGTAGTACA TGAAACTGTT 
    AGGGGTGATC GTCATAATTC TCAATCTTTG TTGCTTGGAT TAACTGAAGG AATAAAACAC 
    CCAGTTTCCA TGGCAATAGC ATCCTATCTC AAAGAAAAAG GTGTTTCTGC TCAAAATGTT 
    TCTAATACAA AAGCTGTGAC TGGTAAGCGG GTAGAAGGAA CATCATACTC TGGTTTGAAG 
    TTACAAGGAG GGAACTGTCG TTGGCTTGGT CATAACAATG ATCCTGACGT TCGAAAAGCC 
    CTTGAACAAG GATATTCTGT ATTCTGTTTT AGTGTTAATG GTTCAGTCAC TGCAGTTTAT 
    GCATTAGAGG ACTCTTTACG GGCAGATGCT GTCTCCACTA TTAACTTGTT ACGCCAAAGA 
    GGGATTTCAC TACACATTTT ATCAGGGGAT GACGATGGAG CCGTTCGTTC TATGGCAGCC 
    CGTCTTGGAA TTGAAAGCTC CAATATTCGT TCTCACGCAA CGCCTGCAGA AAAGAGTGAA 
    TATATTAAGG ATATTGTCGA AGGAAGAAAT TGCGATAGTT CTTCGCAGTC AAAAAGACCG 
    GTTGTTGTTT TTTGCGGTGA CGGAACAAAC GACGCAATTG GTTTGACTCA AGCAACGATT 
    GGAGTTCATA TCAATGAGGG AAGTGAGGTT GCCAAGCTAG CTGCTGATGT AGTTATGTTA 
    AAGCCAAAGC TCAACAATAT TTTGACTATG ATAACTGTAA GTCAAAAGGC CATGTTTAGG 
    GTCAAATTGA ATTTCTTATG GAGTTTTACT TACAACTTAT TTGCTATCCT TTTGGCGGCT 
    GGTGCCTTTG TTGACTTTCA TATTCCACCA GAGTATGCGG GTTTAGGGGA ACTAGTTAGT 
    ATCCTCCCTG TAATTTTTGT AGCTATACTT CTGCGTTATG CAAAGATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome II.
    The EMBO journal13(24)5795-809(1994 Dec)
    Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K


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