PCA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PCA1
Entrez Gene ID	852598
Full Name	cation-transporting P-type ATPase PCA1
Synonyms	CAD2,PAY2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178643.2	NP_009854.2	cation-transporting P-type ATPase PCA1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PCA1	NP_009854.2
Neurospora crassa	NCU07531	XP_959044.1
Magnaporthe oryzae (rice blast fungus)	MGG_10941	XP_003713256.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Determined the roles for the molecular factors of ERAD in dislodging Pca1p from the endoplasmic reticulum (ER). Inactivation of the 20S proteasome leads to accumulation of ubiquitinated Pca1p in the ER membrane, suggesting a role for the proteasome in extraction of Pca1p from the ER.
Title: Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane.

Data indicate that specific cysteine residues in a degron of cation-transporting P-type ATPase PCA1 (Pca1p) sense cadmium.
Title: Cadmium and Secondary Structure-dependent Function of a Degron in the Pca1p Cadmium Exporter.

cadmium resistance is primarily caused by an enhanced function of a metal efflux pump, PCA1.
Title: A tradeoff drives the evolution of reduced metal resistance in natural populations of yeast.

Direct cadmium sensing at the endoplasmic reticulum by a degron in Pca1 leads to an escape of Pca1 from endoplasmic reticulum-associated degradation.
Title: Cadmium-mediated rescue from ER-associated degradation induces expression of its exporter.

Cadmium sensing by cysteine residues within this domain circumvents ubiquitination and degradation of Pca1
Title: Expressional control of a cadmium-transporting P1B-type ATPase by a metal sensing degradation signal.

The following PCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01989	NM_001178643.2 Latest version!	Saccharomyces cerevisiae S288C cation-transporting P-type ATPase PCA1 (PCA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCA1 ( NM_001178643.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009854.2 Saccharomyces cerevisiae S288C cation-transporting P-type ATPase PCA1 (PCA1), partial mRNA.

Price & Availability↑

CloneID	OSi01989
Clone ID Related Accession (Same CDS sequence)	NM_001178643.2
Accession Version	NM_001178643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cation-transporting P-type ATPase PCA1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178643.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGCCGG AAAAACTCTT CTCCGGTTTA GGTACCAGCG ATGGCGAGTA TGGAGTGGTA 
AATAGCGAGA ACATATCAAT AGATGCTATG CAAGATAATA GAGGCGAGTG TCATCGTAGA 
TCCATAGAAA TGCATGCTAA TGACAATCTG GGATTGGTCT CCCAGCGAGA CTGTACCAAT 
CGGCCCAAAA TTACTCCTCA GGAATGTTTG AGTGAGACTG AGCAGATATG CCATCATGGC 
GAGAATAGGA CTAAGGCTGG GTTAGATGTA GACGATGCTG AAACCGGTGG TGATCACACC 
AATGAATCCC GTGTAGATGA ATGTTGTGCT GAGAAAGTGA ACGACACTGA GACTGGCTTG 
GATGTGGACA GCTGTTGCGG CGATGCTCAA ACAGGTGGTG ACCACACCAA TGAATCCTGT 
GTTGATGGAT GCTGCGTTAG AGATTCTTCA GTGATGGTCG AGGAAGTTAC AGGCAGCTGC 
GAAGCTGTAT CTTCTAAAGA ACAATTGCTG ACCAGCTTTG AAGTTGTTCC AAGCAAATCT 
GAAGGTCTGC AATCTATTCA TGATATTAGA GAGACAACTC GCTGCAATAC CAATTCCAAC 
CAACACACAG GGAAGGGTAG GCTTTGCATC GAATCAAGTG ACTCTACACT CAAGAAAAGA 
AGTTGTAAAG TTAGCAGACA GAAAATTGAG GTTTCCAGTA AGCCTGAGTG CTGCAACATT 
TCATGTGTTG AGCGGATTGC ATCTCGTAGC TGTGAAAAGA GGACTTTTAA AGGTAGCACC 
AATGTTGGAA TTTCTGGGAG TAGCTCTACC GACAGTTTAA GCGAGAAGTT CTTCAGCGAA 
CAGTACTCTA GAATGTACAA TCGTTACTCC TCAATTTTGA AAAATTTAGG TTGTATCTGT 
AACTATTTGC GTACTTTGGG AAAAGAATCT TGTTGTCTCC CAAAGGTGCG TTTTTGTAGC 
GGGGAGGGTG CTTCTAAAAA GACAAAATAC TCCTACCGCA ACAGTTCTGG ATGTTTAACA 
AAGAAAAAGA CCCATGGAGA CAAAGAAAGG TTGAGCAATG ACAATGGCCA TGCTGATTTT 
GTTTGTTCTA AAAGTTGTTG CACTAAAATG AAGGATTGTG CTGTTACCTC AACTATTTCT 
GGACACTCTT CGAGTGAAAT TTCAAGAATC GTATCAATGG AACCAATTGA AAATCATCTT 
AATCTTGAGG CAGGATCTAC CGGTACCGAG CACATTGTTC TTAGTGTTTC AGGAATGTCC 
TGTACTGGTT GTGAATCAAA ACTTAAGAAA TCATTTGGCG CGCTCAAATG TGTTCACGGT 
TTGAAGACCA GTTTAATATT ATCGCAAGCT GAATTTAATC TGGATCTTGC TCAGGGATCT 
GTCAAGGATG TTATCAAGCA CTTGAGCAAA ACTACTGAAT TCAAGTACGA ACAGATTTCA 
AATCATGGTT CAACTATAGA TGTTGTTGTT CCTTATGCAG CAAAAGATTT TATTAATGAG 
GAATGGCCAC AAGGTGTCAC AGAGCTGAAA ATTGTTGAGA GAAATATCAT TCGTATTTAC 
TTTGACCCAA AGGTTATAGG TGCCAGAGAT CTTGTCAATG AAGGATGGAG CGTGCCTGTT 
AGTATTGCTC CATTCAGTTG TCATCCAACC ATTGAAGTGG GAAGGAAGCA TTTAGTTCGT 
GTAGGCTGCA CGACCGCTTT ATCCATAATA TTGACGATCC CGATTCTCGT GATGGCTTGG 
GCTCCACAGC TTCGTGAAAA AATTTCCACT ATCTCCGCGT CAATGGTGCT AGCAACTATT 
ATTCAATTTG TTATTGCAGG ACCGTTTTAC TTAAATGCCT TGAAGAGTTT GATATTTTCC 
AGGCTCATTG AAATGGATCT ACTAATAGTT TTAAGCACTA GCGCTGCCTA CATATTTTCG 
ATTGTATCAT TTGGATATTT CGTTGTTGGA CGCCCGTTAT CTACGGAGCA GTTTTTTGAA 
ACCAGCTCTT TGCTTGTAAC TCTCATCATG GTTGGTCGCT TTGTGAGTGA ACTAGCTAGA 
CATAGGGCTG TAAAGTCAAT ATCAGTACGT TCCTTACAGG CCTCTTCTGC TATTCTGGTG 
GATAAAACTG GTAAAGAGAC GGAAATTAAT ATTAGACTTC TCCAATATGG CGATATTTTT 
AAGGTTTTAC CTGATTCAAG AATCCCAACT GATGGAACAG TCATTTCTGG GTCTTCTGAA 
GTCGACGAAG CGTTAATCAC AGGTGAATCT ATGCCAGTCC CGAAAAAGTG TCAATCAATT 
GTTGTTGCAG GATCGGTGAA TGGCACTGGT ACTTTGTTTG TGAAGCTGAG CAAGCTTCCA 
GGGAACAATA CTATTAGTAC CATAGCCACG ATGGTTGATG AAGCAAAATT AACTAAGCCA 
AAGATCCAAA ATATCGCTGA TAAAATTGCA AGTTATTTCG TGCCAACTAT CATTGGAATC 
ACTGTCGTCA CCTTTTGCGT TTGGATAGCG GTTGGAATCC GTGTGGAAAA GCAATCCCGC 
TCCGATGCCG TAATTCAGGC CATTATATAT GCCATTACGG TACTTATCGT TTCGTGTCCC 
TGCGTGATTG GACTTGCCGT TCCTATCGTA TTTGTTATTG CAAGTGGCGT CGCTGCGAAA 
AGAGGGGTAA TCTTCAAATC AGCAGAGAGT ATAGAAGTTG CTCACAACAC TTCGCATGTT 
GTCTTCGATA AAACGGGTAC CCTAACTGAA GGAAAACTTA CTGTAGTACA TGAAACTGTT 
AGGGGTGATC GTCATAATTC TCAATCTTTG TTGCTTGGAT TAACTGAAGG AATAAAACAC 
CCAGTTTCCA TGGCAATAGC ATCCTATCTC AAAGAAAAAG GTGTTTCTGC TCAAAATGTT 
TCTAATACAA AAGCTGTGAC TGGTAAGCGG GTAGAAGGAA CATCATACTC TGGTTTGAAG 
TTACAAGGAG GGAACTGTCG TTGGCTTGGT CATAACAATG ATCCTGACGT TCGAAAAGCC 
CTTGAACAAG GATATTCTGT ATTCTGTTTT AGTGTTAATG GTTCAGTCAC TGCAGTTTAT 
GCATTAGAGG ACTCTTTACG GGCAGATGCT GTCTCCACTA TTAACTTGTT ACGCCAAAGA 
GGGATTTCAC TACACATTTT ATCAGGGGAT GACGATGGAG CCGTTCGTTC TATGGCAGCC 
CGTCTTGGAA TTGAAAGCTC CAATATTCGT TCTCACGCAA CGCCTGCAGA AAAGAGTGAA 
TATATTAAGG ATATTGTCGA AGGAAGAAAT TGCGATAGTT CTTCGCAGTC AAAAAGACCG 
GTTGTTGTTT TTTGCGGTGA CGGAACAAAC GACGCAATTG GTTTGACTCA AGCAACGATT 
GGAGTTCATA TCAATGAGGG AAGTGAGGTT GCCAAGCTAG CTGCTGATGT AGTTATGTTA 
AAGCCAAAGC TCAACAATAT TTTGACTATG ATAACTGTAA GTCAAAAGGC CATGTTTAGG 
GTCAAATTGA ATTTCTTATG GAGTTTTACT TACAACTTAT TTGCTATCCT TTTGGCGGCT 
GGTGCCTTTG TTGACTTTCA TATTCCACCA GAGTATGCGG GTTTAGGGGA ACTAGTTAGT 
ATCCTCCCTG TAATTTTTGT AGCTATACTT CTGCGTTATG CAAAGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009854.2
CDS	1..3651
Translation	MKPEKLFSGLGTSDGEYGVVNSENISIDAMQDNRGECHRRSIEMHANDNLGLVSQRDCTNRPKITPQECLSETEQICHHGENRTKAGLDVDDAETGGDHTNESRVDECCAEKVNDTETGLDVDSCCGDAQTGGDHTNESCVDGCCVRDSSVMVEEVTGSCEAVSSKEQLLTSFEVVPSKSEGLQSIHDIRETTRCNTNSNQHTGKGRLCIESSDSTLKKRSCKVSRQKIEVSSKPECCNISCVERIASRSCEKRTFKGSTNVGISGSSSTDSLSEKFFSEQYSRMYNRYSSILKNLGCICNYLRTLGKESCCLPKVRFCSGEGASKKTKYSYRNSSGCLTKKKTHGDKERLSNDNGHADFVCSKSCCTKMKDCAVTSTISGHSSSEISRIVSMEPIENHLNLEAGSTGTEHIVLSVSGMSCTGCESKLKKSFGALKCVHGLKTSLILSQAEFNLDLAQGSVKDVIKHLSKTTEFKYEQISNHGSTIDVVVPYAAKDFINEEWPQGVTELKIVERNIIRIYFDPKVIGARDLVNEGWSVPVSIAPFSCHPTIEVGRKHLVRVGCTTALSIILTIPILVMAWAPQLREKISTISASMVLATIIQFVIAGPFYLNALKSLIFSRLIEMDLLIVLSTSAAYIFSIVSFGYFVVGRPLSTEQFFETSSLLVTLIMVGRFVSELARHRAVKSISVRSLQASSAILVDKTGKETEINIRLLQYGDIFKVLPDSRIPTDGTVISGSSEVDEALITGESMPVPKKCQSIVVAGSVNGTGTLFVKLSKLPGNNTISTIATMVDEAKLTKPKIQNIADKIASYFVPTIIGITVVTFCVWIAVGIRVEKQSRSDAVIQAIIYAITVLIVSCPCVIGLAVPIVFVIASGVAAKRGVIFKSAESIEVAHNTSHVVFDKTGTLTEGKLTVVHETVRGDRHNSQSLLLGLTEGIKHPVSMAIASYLKEKGVSAQNVSNTKAVTGKRVEGTSYSGLKLQGGNCRWLGHNNDPDVRKALEQGYSVFCFSVNGSVTAVYALEDSLRADAVSTINLLRQRGISLHILSGDDDGAVRSMAARLGIESSNIRSHATPAEKSEYIKDIVEGRNCDSSSQSKRPVVVFCGDGTNDAIGLTQATIGVHINEGSEVAKLAADVVMLKPKLNNILTMITVSQKAMFRVKLNFLWSFTYNLFAILLAAGAFVDFHIPPEYAGLGELVSILPVIFVAILLRYAKI

Target ORF information:

RefSeq Version	NM_001178643.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cation-transporting P-type ATPase PCA1 (PCA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178643.2

ORF Insert Sequence:

ATGAAGCCGG AAAAACTCTT CTCCGGTTTA GGTACCAGCG ATGGCGAGTA TGGAGTGGTA 
AATAGCGAGA ACATATCAAT AGATGCTATG CAAGATAATA GAGGCGAGTG TCATCGTAGA 
TCCATAGAAA TGCATGCTAA TGACAATCTG GGATTGGTCT CCCAGCGAGA CTGTACCAAT 
CGGCCCAAAA TTACTCCTCA GGAATGTTTG AGTGAGACTG AGCAGATATG CCATCATGGC 
GAGAATAGGA CTAAGGCTGG GTTAGATGTA GACGATGCTG AAACCGGTGG TGATCACACC 
AATGAATCCC GTGTAGATGA ATGTTGTGCT GAGAAAGTGA ACGACACTGA GACTGGCTTG 
GATGTGGACA GCTGTTGCGG CGATGCTCAA ACAGGTGGTG ACCACACCAA TGAATCCTGT 
GTTGATGGAT GCTGCGTTAG AGATTCTTCA GTGATGGTCG AGGAAGTTAC AGGCAGCTGC 
GAAGCTGTAT CTTCTAAAGA ACAATTGCTG ACCAGCTTTG AAGTTGTTCC AAGCAAATCT 
GAAGGTCTGC AATCTATTCA TGATATTAGA GAGACAACTC GCTGCAATAC CAATTCCAAC 
CAACACACAG GGAAGGGTAG GCTTTGCATC GAATCAAGTG ACTCTACACT CAAGAAAAGA 
AGTTGTAAAG TTAGCAGACA GAAAATTGAG GTTTCCAGTA AGCCTGAGTG CTGCAACATT 
TCATGTGTTG AGCGGATTGC ATCTCGTAGC TGTGAAAAGA GGACTTTTAA AGGTAGCACC 
AATGTTGGAA TTTCTGGGAG TAGCTCTACC GACAGTTTAA GCGAGAAGTT CTTCAGCGAA 
CAGTACTCTA GAATGTACAA TCGTTACTCC TCAATTTTGA AAAATTTAGG TTGTATCTGT 
AACTATTTGC GTACTTTGGG AAAAGAATCT TGTTGTCTCC CAAAGGTGCG TTTTTGTAGC 
GGGGAGGGTG CTTCTAAAAA GACAAAATAC TCCTACCGCA ACAGTTCTGG ATGTTTAACA 
AAGAAAAAGA CCCATGGAGA CAAAGAAAGG TTGAGCAATG ACAATGGCCA TGCTGATTTT 
GTTTGTTCTA AAAGTTGTTG CACTAAAATG AAGGATTGTG CTGTTACCTC AACTATTTCT 
GGACACTCTT CGAGTGAAAT TTCAAGAATC GTATCAATGG AACCAATTGA AAATCATCTT 
AATCTTGAGG CAGGATCTAC CGGTACCGAG CACATTGTTC TTAGTGTTTC AGGAATGTCC 
TGTACTGGTT GTGAATCAAA ACTTAAGAAA TCATTTGGCG CGCTCAAATG TGTTCACGGT 
TTGAAGACCA GTTTAATATT ATCGCAAGCT GAATTTAATC TGGATCTTGC TCAGGGATCT 
GTCAAGGATG TTATCAAGCA CTTGAGCAAA ACTACTGAAT TCAAGTACGA ACAGATTTCA 
AATCATGGTT CAACTATAGA TGTTGTTGTT CCTTATGCAG CAAAAGATTT TATTAATGAG 
GAATGGCCAC AAGGTGTCAC AGAGCTGAAA ATTGTTGAGA GAAATATCAT TCGTATTTAC 
TTTGACCCAA AGGTTATAGG TGCCAGAGAT CTTGTCAATG AAGGATGGAG CGTGCCTGTT 
AGTATTGCTC CATTCAGTTG TCATCCAACC ATTGAAGTGG GAAGGAAGCA TTTAGTTCGT 
GTAGGCTGCA CGACCGCTTT ATCCATAATA TTGACGATCC CGATTCTCGT GATGGCTTGG 
GCTCCACAGC TTCGTGAAAA AATTTCCACT ATCTCCGCGT CAATGGTGCT AGCAACTATT 
ATTCAATTTG TTATTGCAGG ACCGTTTTAC TTAAATGCCT TGAAGAGTTT GATATTTTCC 
AGGCTCATTG AAATGGATCT ACTAATAGTT TTAAGCACTA GCGCTGCCTA CATATTTTCG 
ATTGTATCAT TTGGATATTT CGTTGTTGGA CGCCCGTTAT CTACGGAGCA GTTTTTTGAA 
ACCAGCTCTT TGCTTGTAAC TCTCATCATG GTTGGTCGCT TTGTGAGTGA ACTAGCTAGA 
CATAGGGCTG TAAAGTCAAT ATCAGTACGT TCCTTACAGG CCTCTTCTGC TATTCTGGTG 
GATAAAACTG GTAAAGAGAC GGAAATTAAT ATTAGACTTC TCCAATATGG CGATATTTTT 
AAGGTTTTAC CTGATTCAAG AATCCCAACT GATGGAACAG TCATTTCTGG GTCTTCTGAA 
GTCGACGAAG CGTTAATCAC AGGTGAATCT ATGCCAGTCC CGAAAAAGTG TCAATCAATT 
GTTGTTGCAG GATCGGTGAA TGGCACTGGT ACTTTGTTTG TGAAGCTGAG CAAGCTTCCA 
GGGAACAATA CTATTAGTAC CATAGCCACG ATGGTTGATG AAGCAAAATT AACTAAGCCA 
AAGATCCAAA ATATCGCTGA TAAAATTGCA AGTTATTTCG TGCCAACTAT CATTGGAATC 
ACTGTCGTCA CCTTTTGCGT TTGGATAGCG GTTGGAATCC GTGTGGAAAA GCAATCCCGC 
TCCGATGCCG TAATTCAGGC CATTATATAT GCCATTACGG TACTTATCGT TTCGTGTCCC 
TGCGTGATTG GACTTGCCGT TCCTATCGTA TTTGTTATTG CAAGTGGCGT CGCTGCGAAA 
AGAGGGGTAA TCTTCAAATC AGCAGAGAGT ATAGAAGTTG CTCACAACAC TTCGCATGTT 
GTCTTCGATA AAACGGGTAC CCTAACTGAA GGAAAACTTA CTGTAGTACA TGAAACTGTT 
AGGGGTGATC GTCATAATTC TCAATCTTTG TTGCTTGGAT TAACTGAAGG AATAAAACAC 
CCAGTTTCCA TGGCAATAGC ATCCTATCTC AAAGAAAAAG GTGTTTCTGC TCAAAATGTT 
TCTAATACAA AAGCTGTGAC TGGTAAGCGG GTAGAAGGAA CATCATACTC TGGTTTGAAG 
TTACAAGGAG GGAACTGTCG TTGGCTTGGT CATAACAATG ATCCTGACGT TCGAAAAGCC 
CTTGAACAAG GATATTCTGT ATTCTGTTTT AGTGTTAATG GTTCAGTCAC TGCAGTTTAT 
GCATTAGAGG ACTCTTTACG GGCAGATGCT GTCTCCACTA TTAACTTGTT ACGCCAAAGA 
GGGATTTCAC TACACATTTT ATCAGGGGAT GACGATGGAG CCGTTCGTTC TATGGCAGCC 
CGTCTTGGAA TTGAAAGCTC CAATATTCGT TCTCACGCAA CGCCTGCAGA AAAGAGTGAA 
TATATTAAGG ATATTGTCGA AGGAAGAAAT TGCGATAGTT CTTCGCAGTC AAAAAGACCG 
GTTGTTGTTT TTTGCGGTGA CGGAACAAAC GACGCAATTG GTTTGACTCA AGCAACGATT 
GGAGTTCATA TCAATGAGGG AAGTGAGGTT GCCAAGCTAG CTGCTGATGT AGTTATGTTA 
AAGCCAAAGC TCAACAATAT TTTGACTATG ATAACTGTAA GTCAAAAGGC CATGTTTAGG 
GTCAAATTGA ATTTCTTATG GAGTTTTACT TACAACTTAT TTGCTATCCT TTTGGCGGCT 
GGTGCCTTTG TTGACTTTCA TATTCCACCA GAGTATGCGG GTTTAGGGGA ACTAGTTAGT 
ATCCTCCCTG TAATTTTTGT AGCTATACTT CTGCGTTATG CAAAGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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PCA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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