DUG2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DUG2
Entrez Gene ID	852584
Full Name	glutamine amidotransferase subunit DUG2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178629.3	NP_009840.3	glutamine amidotransferase subunit DUG2

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_04227	XP_003719586.1
Neurospora crassa	NCU05622	XP_960166.2
Saccharomyces cerevisiae (baker's yeast)	DUG2	NP_009840.3
Kluyveromyces lactis	KLLA0F01991g	XP_455170.1
Eremothecium gossypii	AGOS_AEL154C	NP_984707.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dug2p and Dug3p were required together for the cleavage of glutathione into glutamate and Cys-Gly.
Title: Glutathione degradation by the alternative pathway (DUG pathway) in Saccharomyces cerevisiae is initiated by (Dug2p-Dug3p)2 complex, a novel glutamine amidotransferase (GATase) enzyme acting on glutathione.

The following DUG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DUG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01976	NM_001178629.3 Latest version!	Saccharomyces cerevisiae S288C glutamine amidotransferase subunit DUG2 (DUG2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DUG2 ( NM_001178629.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009840.3 Saccharomyces cerevisiae S288C glutamine amidotransferase subunit DUG2 (DUG2), partial mRNA.

Price & Availability↑

CloneID	OSi01976
Clone ID Related Accession (Same CDS sequence)	NM_001178629.3
Accession Version	NM_001178629.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	glutamine amidotransferase subunit DUG2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178629.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTACGATA GCAGGGGTGT CGCATTGCAC TCTGAACTAA TTCATAGGTG GAATCACGCT 
TTTTCCATCT TATCCATTGT TGCATTTCCA AAAAAAAGAT TGTTGTTTGC TGGTAGCCAA 
GACTCCAAGA TATTGGTTTT TGATCTTCCC ACATATAATC TAATACATAC GATTAGACTA 
GGCGAATCAC AAGAGGAAAC GCATACCAGA TCTTCGGTTT TGTGCTTGAC AGGATCAGAA 
GATGAAAACT TTTTATTTTC AGGCGGCGCA GATTCTTTGG TGAGAATTTG GTCCATTGGC 
GAAAAGACCA TTAGAGATGA CTTTCTACCT GTTACTGAAA TAGCCACTGT TTACTCAGTG 
ACCGATATTG GTGATATTTT CTCATTGGCA TATTTGGATT CGTTGGAAAC TATTGTCTTT 
GGCTGTCAAA ATGCAAGCTT ACTATATGTT GAGAACTTGA TTCAGAAGAT TGAAAAAAAA 
TCCTCTGATG GAGTAGAAAA TATCAATAAA TTACCGCATA GAAGATATGA TAAGTTCTTT 
GATTCGTTAG GTCCGACTGG ATATAGTTCA AATTCATTAT CTCAAACTTC ATTGACCTCT 
CTACAAGAAA ACTGTGGCGC TGCCATCATA GAGGTTCCCT CCGAAAACAT TATTAAATAT 
GCACATTATG GGTTTATTTA TTCTATTAAT AAATTGTGTC CCAGGTTTAA TCAGTTATTG 
GAAAAAAGCT CACGAACTTC TGGTGCAGAG CACATAATTT CATCCGCTGG TGATGGAATA 
AGCAAGCTTT GGGAGTTCTC TAAAGATAAA GGGCAAAACA CTGTTAAAAT CTCCTTGATA 
AACGATAAAA TCGACAATGA AGATAGCGTC ATATCCCAAA CTATTGAGTT CCCATTTTTA 
TATTGCGGGT TGACTGACGG GATTATAAAA ATCTGGGACT TAAATACTCA GCAAATAATC 
TCAACTCTGA AAACAAAGCA TGAATCGGAC GTCATTTCAA TATCAGTTTA TATGGACCAT 
GTATTTGCCA TTGATGAATC AGGCATCACG CATTTTTACC AGAACCAAGT TAACCATTGG 
AACCCTCAAC AAGGAAAAAT ATTAAGCTCA GAGATTTTCA GTAAATCAAA TGCTGGATCT 
GTTAGTTTAT TAACGGGTGG TAGCGATGGG TCATTGACTC TCTGGGATAT AACCTCATTA 
TTATCTGCGG TGCCTCTGTC AAGCAACTCA CCCATCAATG CATCATCTAC ATTACAAACC 
ACCAATTTAT GGGCCGCCTA TCAATCTGCC TCTCTAAATA ACGAGGAGAT GTTAAACACA 
CTGAGAGAAC TTATTTCTTT CCAGACAGTG TCTCAAAGCA AAGACACTAC TAATACTTTG 
TCATTAAGGC GTTGTGCAAT CTACCTGCAA CAATTATTCT TGAAGTTTGG AGCTACAAAC 
TCCCAATTAT TTCCATTGCC TGATGGTGGT AATCCTGTTG TATTCGCATA TTTTCAAGGG 
AATGGGAAAG TTTCACAGGT GAAGGGTGCC AAGAAAAAGC GTATTCTATG GTATGGGCAT 
TATGACGTTA TATCGTCTGG AAATACTTTC AACTGGAATA CCGACCCATT TACTTTAACT 
TGTGAAAACG GATACTTAAA AGGTCGAGGA GTATCAGATA ATAAAGGGCC ATTGGTGAGC 
GCTATTCATA GTGTGGCGTA TTTGTTTCAA CAGGGAGAAT TGGTGAACGA TGTCGTATTT 
TTGGTGGAAG GAAGTGAAGA AATCGGATCT GCTAGTTTGA AGCAAGTTTG TGAAAAGTAC 
CATGATATTA TTGGAAAAGA TATTGACTGG ATTTTATTAA GTAATTCTAC TTGGGTTGAT 
CAAGAACATC CATGCTTGAA TTATGGATTG AGAGGTGTTA TTAATGCCCA AATAAAAGTC 
TGGAGCGATA AGCCTGACGG ACATTCTGGT CTTAACGGTG GTGTTTATGA TGAACCTATG 
GTTAATTTAG TGAAAATCGT GTCCAAACTA CAAAACGAAC AAAATGAAAT TATGATTCCA 
AATTTTTATT CACCATTGAA AGATCTGACT GAAGAAGAAT ATCAAAGGTT TCAGAAAATT 
ACCGAGCTTG CAAATATCGA CGAAAATACT ACTGTTCAAG ATTTAATTAC CAACTGGACT 
AAGCCTTCCT TGTCTATGAC AACAGTCAAG TTTAGCGGTC CTGGTAACAT AACAGTTATT 
CCAAAAAGTG TCACCATGGG TATCTCCATC AGGTTAGTAC CTGAGCAAAG TGTAGAACAA 
GTCAAGAGAG ATCTCAAAGC GTATTTAGAA GAAAGTTTCA AGCAATTGAA ATCTCAAAAT 
CATCTAGAAA TTAAAGTTTT AAACGAAGCA GAAGGTTGGT TGGGTGACCC AACAAATCAT 
GCATACCAAA TATTAAAGGA CGAAATTACT ACTGCATGGG ACGTAGAACC ATTGCTAGTA 
AGAGAAGGAG GTTCTATTTC ATGTTTGAGA ATGTTGGAAA GAATATTCGA CGCGCCAGCT 
GTTCAAATAC CATGTGGCCA ATCCACTGAC AATGGCCATT TAGCCAACGA AAATCTCAGA 
ATCAAAAATT GGTCTAATTT GACTGAGATT TTGTCTAAAG TATTCAATAG GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009840.3
CDS	1..2637
Translation	MYDSRGVALHSELIHRWNHAFSILSIVAFPKKRLLFAGSQDSKILVFDLPTYNLIHTIRLGESQEETHTRSSVLCLTGSEDENFLFSGGADSLVRIWSIGEKTIRDDFLPVTEIATVYSVTDIGDIFSLAYLDSLETIVFGCQNASLLYVENLIQKIEKKSSDGVENINKLPHRRYDKFFDSLGPTGYSSNSLSQTSLTSLQENCGAAIIEVPSENIIKYAHYGFIYSINKLCPRFNQLLEKSSRTSGAEHIISSAGDGISKLWEFSKDKGQNTVKISLINDKIDNEDSVISQTIEFPFLYCGLTDGIIKIWDLNTQQIISTLKTKHESDVISISVYMDHVFAIDESGITHFYQNQVNHWNPQQGKILSSEIFSKSNAGSVSLLTGGSDGSLTLWDITSLLSAVPLSSNSPINASSTLQTTNLWAAYQSASLNNEEMLNTLRELISFQTVSQSKDTTNTLSLRRCAIYLQQLFLKFGATNSQLFPLPDGGNPVVFAYFQGNGKVSQVKGAKKKRILWYGHYDVISSGNTFNWNTDPFTLTCENGYLKGRGVSDNKGPLVSAIHSVAYLFQQGELVNDVVFLVEGSEEIGSASLKQVCEKYHDIIGKDIDWILLSNSTWVDQEHPCLNYGLRGVINAQIKVWSDKPDGHSGLNGGVYDEPMVNLVKIVSKLQNEQNEIMIPNFYSPLKDLTEEEYQRFQKITELANIDENTTVQDLITNWTKPSLSMTTVKFSGPGNITVIPKSVTMGISIRLVPEQSVEQVKRDLKAYLEESFKQLKSQNHLEIKVLNEAEGWLGDPTNHAYQILKDEITTAWDVEPLLVREGGSISCLRMLERIFDAPAVQIPCGQSTDNGHLANENLRIKNWSNLTEILSKVFNRL

Target ORF information:

RefSeq Version	NM_001178629.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C glutamine amidotransferase subunit DUG2 (DUG2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178629.3

ORF Insert Sequence:

ATGTACGATA GCAGGGGTGT CGCATTGCAC TCTGAACTAA TTCATAGGTG GAATCACGCT 
TTTTCCATCT TATCCATTGT TGCATTTCCA AAAAAAAGAT TGTTGTTTGC TGGTAGCCAA 
GACTCCAAGA TATTGGTTTT TGATCTTCCC ACATATAATC TAATACATAC GATTAGACTA 
GGCGAATCAC AAGAGGAAAC GCATACCAGA TCTTCGGTTT TGTGCTTGAC AGGATCAGAA 
GATGAAAACT TTTTATTTTC AGGCGGCGCA GATTCTTTGG TGAGAATTTG GTCCATTGGC 
GAAAAGACCA TTAGAGATGA CTTTCTACCT GTTACTGAAA TAGCCACTGT TTACTCAGTG 
ACCGATATTG GTGATATTTT CTCATTGGCA TATTTGGATT CGTTGGAAAC TATTGTCTTT 
GGCTGTCAAA ATGCAAGCTT ACTATATGTT GAGAACTTGA TTCAGAAGAT TGAAAAAAAA 
TCCTCTGATG GAGTAGAAAA TATCAATAAA TTACCGCATA GAAGATATGA TAAGTTCTTT 
GATTCGTTAG GTCCGACTGG ATATAGTTCA AATTCATTAT CTCAAACTTC ATTGACCTCT 
CTACAAGAAA ACTGTGGCGC TGCCATCATA GAGGTTCCCT CCGAAAACAT TATTAAATAT 
GCACATTATG GGTTTATTTA TTCTATTAAT AAATTGTGTC CCAGGTTTAA TCAGTTATTG 
GAAAAAAGCT CACGAACTTC TGGTGCAGAG CACATAATTT CATCCGCTGG TGATGGAATA 
AGCAAGCTTT GGGAGTTCTC TAAAGATAAA GGGCAAAACA CTGTTAAAAT CTCCTTGATA 
AACGATAAAA TCGACAATGA AGATAGCGTC ATATCCCAAA CTATTGAGTT CCCATTTTTA 
TATTGCGGGT TGACTGACGG GATTATAAAA ATCTGGGACT TAAATACTCA GCAAATAATC 
TCAACTCTGA AAACAAAGCA TGAATCGGAC GTCATTTCAA TATCAGTTTA TATGGACCAT 
GTATTTGCCA TTGATGAATC AGGCATCACG CATTTTTACC AGAACCAAGT TAACCATTGG 
AACCCTCAAC AAGGAAAAAT ATTAAGCTCA GAGATTTTCA GTAAATCAAA TGCTGGATCT 
GTTAGTTTAT TAACGGGTGG TAGCGATGGG TCATTGACTC TCTGGGATAT AACCTCATTA 
TTATCTGCGG TGCCTCTGTC AAGCAACTCA CCCATCAATG CATCATCTAC ATTACAAACC 
ACCAATTTAT GGGCCGCCTA TCAATCTGCC TCTCTAAATA ACGAGGAGAT GTTAAACACA 
CTGAGAGAAC TTATTTCTTT CCAGACAGTG TCTCAAAGCA AAGACACTAC TAATACTTTG 
TCATTAAGGC GTTGTGCAAT CTACCTGCAA CAATTATTCT TGAAGTTTGG AGCTACAAAC 
TCCCAATTAT TTCCATTGCC TGATGGTGGT AATCCTGTTG TATTCGCATA TTTTCAAGGG 
AATGGGAAAG TTTCACAGGT GAAGGGTGCC AAGAAAAAGC GTATTCTATG GTATGGGCAT 
TATGACGTTA TATCGTCTGG AAATACTTTC AACTGGAATA CCGACCCATT TACTTTAACT 
TGTGAAAACG GATACTTAAA AGGTCGAGGA GTATCAGATA ATAAAGGGCC ATTGGTGAGC 
GCTATTCATA GTGTGGCGTA TTTGTTTCAA CAGGGAGAAT TGGTGAACGA TGTCGTATTT 
TTGGTGGAAG GAAGTGAAGA AATCGGATCT GCTAGTTTGA AGCAAGTTTG TGAAAAGTAC 
CATGATATTA TTGGAAAAGA TATTGACTGG ATTTTATTAA GTAATTCTAC TTGGGTTGAT 
CAAGAACATC CATGCTTGAA TTATGGATTG AGAGGTGTTA TTAATGCCCA AATAAAAGTC 
TGGAGCGATA AGCCTGACGG ACATTCTGGT CTTAACGGTG GTGTTTATGA TGAACCTATG 
GTTAATTTAG TGAAAATCGT GTCCAAACTA CAAAACGAAC AAAATGAAAT TATGATTCCA 
AATTTTTATT CACCATTGAA AGATCTGACT GAAGAAGAAT ATCAAAGGTT TCAGAAAATT 
ACCGAGCTTG CAAATATCGA CGAAAATACT ACTGTTCAAG ATTTAATTAC CAACTGGACT 
AAGCCTTCCT TGTCTATGAC AACAGTCAAG TTTAGCGGTC CTGGTAACAT AACAGTTATT 
CCAAAAAGTG TCACCATGGG TATCTCCATC AGGTTAGTAC CTGAGCAAAG TGTAGAACAA 
GTCAAGAGAG ATCTCAAAGC GTATTTAGAA GAAAGTTTCA AGCAATTGAA ATCTCAAAAT 
CATCTAGAAA TTAAAGTTTT AAACGAAGCA GAAGGTTGGT TGGGTGACCC AACAAATCAT 
GCATACCAAA TATTAAAGGA CGAAATTACT ACTGCATGGG ACGTAGAACC ATTGCTAGTA 
AGAGAAGGAG GTTCTATTTC ATGTTTGAGA ATGTTGGAAA GAATATTCGA CGCGCCAGCT 
GTTCAAATAC CATGTGGCCA ATCCACTGAC AATGGCCATT TAGCCAACGA AAATCTCAGA 
ATCAAAAATT GGTCTAATTT GACTGAGATT TTGTCTAAAG TATTCAATAG GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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DUG2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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