SAF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SAF1
Entrez Gene ID	852583
Full Name	SCF ubiquitin ligase complex subunit SAF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178628.3	NP_009839.4	SCF ubiquitin ligase complex subunit SAF1

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AEL155C	NP_984706.1
Saccharomyces cerevisiae (baker's yeast)	SAF1	NP_009839.4
Kluyveromyces lactis	KLLA0F01969g	XP_455169.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Saf1 does not require a conventional degron as do other F-box proteins but instead recognizes the catalytic site itself.
Title: Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1.

Saf1 selectively promotes ubiquitination of the unprocessed form of the zymogen. This suggests that Saf1 is part of a pathway that targets protein precursors for proteasomal degradation.
Title: Ubiquitin ligase trapping identifies an SCF(Saf1) pathway targeting unprocessed vacuolar/lysosomal proteins.

Saf1p is being a bona fide Skp1-Cullin-F-box subunit
Title: Skp1-Cullin-F-box-dependent degradation of Aah1p requires its interaction with the F-box protein Saf1p.

Saf1p targets Aah1p for proteasome-dependent degradation upon entry into quiescence.
Title: Proteasome- and SCF-dependent degradation of yeast adenine deaminase upon transition from proliferation to quiescence requires a new F-box protein named Saf1p.

The following SAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01975	NM_001178628.3 Latest version!	Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit SAF1 (SAF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SAF1 ( NM_001178628.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009839.4 Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit SAF1 (SAF1), partial mRNA.

Price & Availability↑

CloneID	OSi01975
Clone ID Related Accession (Same CDS sequence)	NM_001178628.3
Accession Version	NM_001178628.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SCF ubiquitin ligase complex subunit SAF1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178628.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGAAG TTGAGAGCAG AGAAAAGGAG CCTGACGCAG GATTGTCCCC AGATATCGTT 
CAAGCTACGC TACCATTTTT AAGCAGTGAT GATATTAAGA ACCTCTCGCA GACAAATAAG 
TATTATAATA CATTACTAGA TTTTGACCAC TCTAAAATTT TGTGGCATGA ACTATTTCAT 
AAGGCATTTG GGACATTAAA GACAAATGAC GAGCCTTTCC AGGGTCGAAA TTCGGCGGAA 
TTTAAGACCT GCACAGAGAC GATTTTAAGA GAAGCATTCC CCACCTTGTC ATGGCAGGAA 
GTATACCAAT TACGGGCATA TGATGCAAAG TTTTATAGTT GGGGTTATTT AAAGCATGGA 
AGGTTGGGCT ATACCGCGAG TTCCAATAAT GAACTTGTCG CCACTTCACT AAACGGGCCA 
AGTCCGCGGT TCAAATATGG TGTCAATACC CCCACTGAAG TTCCTTGGTT TAACAGTAGG 
ACAACATCTA GAACTAGTAA CTTCACTCCT TCAGAGGACC CGTTAAGCGC CATCAAGAAG 
GATGGAGACG AAATCATAGC TCAAGTATCT AGTGGTGGTT TTTCTTTCCA AATATTGACT 
GAGTCAGGTA ACCTTTACAG CAGCGGATCC ACTTTTTCTG GAGGGTTAAA AGGACCAGGT 
CCCAGTGGTT CACAGCATGA TTATAATCCC TTCAGAGAAA TGATTCACAA TATGGAAAGA 
TCGTATCCGC GGATCACTAG CCGCAGTAAT GGGAGTACTG TCAATACCAC GGGTACATTT 
TCTGGAAGGA GAATGAGCGG TAGCCATCCA TCTACTGCTT ATGAACCAGG AAATGCAACT 
ACAGCTCAGC ATATTACCAT AGATAGCGGA GGTGCGCCTG CAGCCTCTCC CGGAGGGAGC 
CATAGTGGTG TCCCTAGAAC TACAATGCCT TCAATGGGGC CGCATGAGAA TATTTACAGC 
CAGATAGAAA TGCTGGAACG AAGTGCTAAC AAGGCTGTAC CAGGAAATAA TCATATCAGG 
AGAATGTTCG CAAGAAATTC TTTCCCATTA TATAGTGGTA GGGATGAAAA TTTAGGATCA 
TTTAATGATA TTCAATTTGT CGCGGTAAGC TCTGGGAGAA GTCATTTCCT AGCAATGGAT 
ACAGATAACA ATATTTATTC TTGGGACTCA ACGGAATCTG ACCAAGGTGT CAAAATTGAG 
TTTGCTAACC TACCTAGTCG TGCTACGAAT CCTATTTTGA AAATTGCTAG TGGTTGGAAT 
TTCAATTGTT GCTATATATA TAAGGTCGGG TTGGTTGCAT GGAAAGAAAG AGATGCTATT 
CAAAAGGGCG AGAGCTTTGC TTTTGCAAAA TACGAGATTG TCCCGAATAC GAATGATGTT 
AATGGAGACT CCAGGATTGT CGACTTTGCA TGTTTACAAG ATAACTGCGT TTTCTTCATT 
AATAACAACG GCGATAAACT ATGGAAGTAC CACAATGGAC TGAACCAGAT AGTAGATTTA 
AATATTGTCG GAAAACTGTG CAAAATTAAC GCCTGTTTTG CTTCATTAGT CCTCTTCACC 
GATACTCACT GTTATACTTT GAAAGTAACA AATGGAGATG TCGATAAAGA TAGTTTAACG 
GAGCTAGACA TCAATGAAAA TGTTATCTCC GTTGCAAGTG GTGACTATCA CACGGTGGCA 
CTAACCGAAC GCGGTCATTT GTATTCATGG GGTATTGAAA GTCAGGATTG TGGGTGCTTA 
GGATTGGGCC CTTCTGAAAA AATAGTTAAC GAGCTACATA TTGGCAATTG GGAAGGGCAA 
AGAAACATTA GGGTCGTTAA GCCCACGAAA ATAGAACTAC CTGAAGATTA TATTTGTGTC 
AGTGTAACTG CTGGTGGCTG GCAAACCGGT GCATTAATCA TCAAGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009839.4
CDS	1..1914
Translation	MSEVESREKEPDAGLSPDIVQATLPFLSSDDIKNLSQTNKYYNTLLDFDHSKILWHELFHKAFGTLKTNDEPFQGRNSAEFKTCTETILREAFPTLSWQEVYQLRAYDAKFYSWGYLKHGRLGYTASSNNELVATSLNGPSPRFKYGVNTPTEVPWFNSRTTSRTSNFTPSEDPLSAIKKDGDEIIAQVSSGGFSFQILTESGNLYSSGSTFSGGLKGPGPSGSQHDYNPFREMIHNMERSYPRITSRSNGSTVNTTGTFSGRRMSGSHPSTAYEPGNATTAQHITIDSGGAPAASPGGSHSGVPRTTMPSMGPHENIYSQIEMLERSANKAVPGNNHIRRMFARNSFPLYSGRDENLGSFNDIQFVAVSSGRSHFLAMDTDNNIYSWDSTESDQGVKIEFANLPSRATNPILKIASGWNFNCCYIYKVGLVAWKERDAIQKGESFAFAKYEIVPNTNDVNGDSRIVDFACLQDNCVFFINNNGDKLWKYHNGLNQIVDLNIVGKLCKINACFASLVLFTDTHCYTLKVTNGDVDKDSLTELDINENVISVASGDYHTVALTERGHLYSWGIESQDCGCLGLGPSEKIVNELHIGNWEGQRNIRVVKPTKIELPEDYICVSVTAGGWQTGALIIKKH

Target ORF information:

RefSeq Version	NM_001178628.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit SAF1 (SAF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178628.3

ORF Insert Sequence:

ATGAGTGAAG TTGAGAGCAG AGAAAAGGAG CCTGACGCAG GATTGTCCCC AGATATCGTT 
CAAGCTACGC TACCATTTTT AAGCAGTGAT GATATTAAGA ACCTCTCGCA GACAAATAAG 
TATTATAATA CATTACTAGA TTTTGACCAC TCTAAAATTT TGTGGCATGA ACTATTTCAT 
AAGGCATTTG GGACATTAAA GACAAATGAC GAGCCTTTCC AGGGTCGAAA TTCGGCGGAA 
TTTAAGACCT GCACAGAGAC GATTTTAAGA GAAGCATTCC CCACCTTGTC ATGGCAGGAA 
GTATACCAAT TACGGGCATA TGATGCAAAG TTTTATAGTT GGGGTTATTT AAAGCATGGA 
AGGTTGGGCT ATACCGCGAG TTCCAATAAT GAACTTGTCG CCACTTCACT AAACGGGCCA 
AGTCCGCGGT TCAAATATGG TGTCAATACC CCCACTGAAG TTCCTTGGTT TAACAGTAGG 
ACAACATCTA GAACTAGTAA CTTCACTCCT TCAGAGGACC CGTTAAGCGC CATCAAGAAG 
GATGGAGACG AAATCATAGC TCAAGTATCT AGTGGTGGTT TTTCTTTCCA AATATTGACT 
GAGTCAGGTA ACCTTTACAG CAGCGGATCC ACTTTTTCTG GAGGGTTAAA AGGACCAGGT 
CCCAGTGGTT CACAGCATGA TTATAATCCC TTCAGAGAAA TGATTCACAA TATGGAAAGA 
TCGTATCCGC GGATCACTAG CCGCAGTAAT GGGAGTACTG TCAATACCAC GGGTACATTT 
TCTGGAAGGA GAATGAGCGG TAGCCATCCA TCTACTGCTT ATGAACCAGG AAATGCAACT 
ACAGCTCAGC ATATTACCAT AGATAGCGGA GGTGCGCCTG CAGCCTCTCC CGGAGGGAGC 
CATAGTGGTG TCCCTAGAAC TACAATGCCT TCAATGGGGC CGCATGAGAA TATTTACAGC 
CAGATAGAAA TGCTGGAACG AAGTGCTAAC AAGGCTGTAC CAGGAAATAA TCATATCAGG 
AGAATGTTCG CAAGAAATTC TTTCCCATTA TATAGTGGTA GGGATGAAAA TTTAGGATCA 
TTTAATGATA TTCAATTTGT CGCGGTAAGC TCTGGGAGAA GTCATTTCCT AGCAATGGAT 
ACAGATAACA ATATTTATTC TTGGGACTCA ACGGAATCTG ACCAAGGTGT CAAAATTGAG 
TTTGCTAACC TACCTAGTCG TGCTACGAAT CCTATTTTGA AAATTGCTAG TGGTTGGAAT 
TTCAATTGTT GCTATATATA TAAGGTCGGG TTGGTTGCAT GGAAAGAAAG AGATGCTATT 
CAAAAGGGCG AGAGCTTTGC TTTTGCAAAA TACGAGATTG TCCCGAATAC GAATGATGTT 
AATGGAGACT CCAGGATTGT CGACTTTGCA TGTTTACAAG ATAACTGCGT TTTCTTCATT 
AATAACAACG GCGATAAACT ATGGAAGTAC CACAATGGAC TGAACCAGAT AGTAGATTTA 
AATATTGTCG GAAAACTGTG CAAAATTAAC GCCTGTTTTG CTTCATTAGT CCTCTTCACC 
GATACTCACT GTTATACTTT GAAAGTAACA AATGGAGATG TCGATAAAGA TAGTTTAACG 
GAGCTAGACA TCAATGAAAA TGTTATCTCC GTTGCAAGTG GTGACTATCA CACGGTGGCA 
CTAACCGAAC GCGGTCATTT GTATTCATGG GGTATTGAAA GTCAGGATTG TGGGTGCTTA 
GGATTGGGCC CTTCTGAAAA AATAGTTAAC GAGCTACATA TTGGCAATTG GGAAGGGCAA 
AGAAACATTA GGGTCGTTAA GCCCACGAAA ATAGAACTAC CTGAAGATTA TATTTGTGTC 
AGTGTAACTG CTGGTGGCTG GCAAACCGGT GCATTAATCA TCAAGAAGCA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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SAF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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