RIF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RIF1
Entrez Gene ID	852578
Full Name	DNA-binding protein RIF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178623.4	NP_009834.4	DNA-binding protein RIF1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Engagement of the Rif1 N-terminal domain with telomeres proved essential for checkpoint control and telomere length regulation. Unexpectedly, Rif1 N-terminal domain also promoted NHEJ repair at DNA breaks in yeast, revealing a conserved role of Rif1 in DNA repair.
Title: Rif1 maintains telomeres and mediates DNA repair by encasing DNA ends.

These data suggest that the repression of origin activation by Rif1-Glc7 is important to avoid the deleterious accumulation of stalled replication forks at the rDNA RFB, which become lethal when fork stability is compromised. Finally, we show that Rif1-Glc7, unlike Sir2, has an important effect on origin firing outside of the rDNA locus that serves to prevent activation of the DNA replication checkpoint
Title: Budding Yeast Rif1 Controls Genome Integrity by Inhibiting rDNA Replication.

Rif1 is phosphorylated by Tel1 during early replication.Tel1 is required for phosphorylation of Rif1 Serine-1308.
Title: At short telomeres Tel1 directs early replication and phosphorylates Rif1.

Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection.
Title: Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection.

Rif1 mechanism regulates origin timing irrespective of proximity to a chromosome end.
Title: Rif1 regulates initiation timing of late replication origins throughout the S. cerevisiae genome.

The following RIF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01971	NM_001178623.4 Latest version!	Saccharomyces cerevisiae S288C DNA-binding protein RIF1 (RIF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIF1 ( NM_001178623.4 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009834.4 Saccharomyces cerevisiae S288C DNA-binding protein RIF1 (RIF1), partial mRNA.

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CloneID	OSi01971
Clone ID Related Accession (Same CDS sequence)	NM_001178623.4
Accession Version	NM_001178623.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-binding protein RIF1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178623.3. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGAAAG ATTTTTCAGA TAAAAAGAAA CATACGATAG ATCGAATTGA TCAGCATATC 
CTCCGTAGGT CTCAACACGA TAATTATTCC AATGGAAGCT CTCCGTGGAT GAAAACTAAC 
TTGCCACCTC CATCTCCACA AGCTCATATG CATATACAAA GCGATCTATC GCCAACGCCA 
AAGAGGCGAA AGCTAGCGTC TTCAAGTGAT TGCGAGAATA AACAATTTGA TTTATCAGCT 
ATTAACAAGA ATTTATATCC AGAAGATACA GGAAGCAGGC TAATGCAAAG TCTTCCAGAG 
TTGTCTGCCT CTAATAGTGA TAATGTATCA CCGGTTACAA AAAGTGTAGC TTTTTCTGAT 
AGGATAGAAT CATCACCGAT TTACCGCATA CCAGGTTCCT CACCTAAACC CTCTCCATCA 
AGCAAACCAG GAAAATCAAT TTTGAGGAAT AGGTTGCCTT CAGTGAGGAC TGTAAGCGAC 
TTGAGTTACA ATAAACTCCA ATATACCCAA CACAAATTAC ACAACGGTAA TATTTTCACA 
TCACCTTATA AGGAAACAAG GGTAAATCCT CGCGCCTTAG AGTATTGGGT GAGTGGTGAG 
ATCCATGGTT TAGTTGATAA TGAAAGTGTT TCGGAGTTTA AAGAAATTAT TGAGGGCGGA 
TTGGGCATCT TGCGACAAGA ATCTGAAGAT TACGTCGCGA GGAGGTTTGA GGTATACGCA 
ACCTTTAATA ATATTATACC CATTCTAACC ACTAAAAATG TTAACGAGGT TGATCAGAAA 
TTCAATATTC TAATTGTGAA TATAGAAAGT ATCATTGAAA TATGCATACC GCATCTACAA 
ATAGCACAGG ACACATTATT AAGTAGTAGT GAAAAAAAAA ATCCATTTGT AATCAGACTA 
TATGTTCAAA TAGTTCGATT CTTCAGTGCT ATTATGTCTA ATTTCAAAAT CGTTAAGTGG 
CTAACGAAAA GGCCAGATTT GGTTAATAAG TTAAAAGTTA TTTACAGGTG GACCACCGGT 
GCGCTAAGAA ATGAAAATTC AAACAAGATA ATCATAACAG CACAAGTCTC TTTCTTGAGA 
GACGAAAAAT TTGGTACTTT TTTCTTGAGC AACGAAGAAA TTAAACCTAT CATTAGCACT 
TTCACTGAAA TAATGGAAAT CAATAGTCAC AACCTAATAT ATGAAAAATT GCTACTTATA 
AGGGGATTTT TGAGCAAGTA TCCAAAATTA ATGATTGAGA CGGTAACTTC TTGGTTACCA 
GGTGAGGTAC TACCTAGGAT TATTATCGGA GATGAAATTT ACTCCATGAA AATTCTCATA 
ACTTCAATAG TTGTTTTACT GGAACTACTA AAAAAATGCT TAGATTTTGT TGATGAACAT 
GAAAGGATTT ACCAGTGTAT CATGCTATCT CCAGTATGCG AAACAATCCC GGAAAAATTT 
TTATCTAAAC TACCGTTAAA TTCATATGAC AGCGCTAATT TAGATAAAGT GACAATTGGT 
CATCTTCTTA CACAGCAGAT AAAAAACTAT ATTGTGGTCA AAAATGACAA TAAAATAGCA 
ATGGATTTGT GGCTTTCAAT GACCGGATTA CTATACGATA GCGGAAAGAG AGTTTATGAT 
TTGACATCAG AATCTAACAA AGTTTGGTTT GATTTAAATA ACCTGTGCTT TATCAATAAT 
CACCCCAAGA CTCGCTTAAT GTCCATCAAA GTTTGGAGAA TTATTACCTA CTGCATATGC 
ACGAAAATAT CACAGAAAAA TCAGGAAGGA AACAAAAGTT TACTGTCACT TTTACGAACC 
CCTTTTCAAA TGACGCTTCC TTATGTGAAT GATCCAAGCG CTAGAGAAGG GATCATCTAC 
CACCTTTTAG GTGTTGTTTA CACTGCATTT ACAAGTAATA AGAATTTGTC AACAGATATG 
TTTGAACTAT TTTGGGACCA CCTAATAACC CCGATTTATG AAGATTATGT TTTCAAATAT 
GATTCGATTC ACTTGCAAAA TGTACTCTTC ACAGTTTTGC ATTTGTTAAT TGGGGGAAAG 
AATGCAGATG TTGCATTGGA AAGAAAATAT AAAAAGCATA TTCACCCAAT GAGTGTTATA 
GCCTCAGAAG GTGTAAAGTT AAAAGACATT TCAAGCCTGC CCCCGCAGAT CATCAAGCGT 
GAATACGATA AAATCATGAA AGTTGTGTTC CAGGCTGTTG AAGTAGCCAT TTCTAACGTT 
AATTTGGCTC ATGATCTGAT CCTTACATCT TTGAAGCATC TTCCAGAGGA TAGAAAGGAC 
CAAACTCATT TAGAATCCTT TAGCAGTCTT ATATTAAAAG TCACGCAAAA CAACAAAGAT 
ACACCCATTT TTCGTGACTT TTTTGGTGCT GTTACTTCAT CGTTTGTTTA TACATTTTTG 
GACCTGTTTT TAAGAAAGAA TGATTCATCA TTAGTAAATT TCAATATCCA AATTTCCAAA 
GTTGGTATTT CGCAAGGAAA CATGACCCTT GATCTTTTAA AAGATGTTAT CAGGAAAGCT 
CGAAACGAAA CATCTGAATT TCTGATAATA GAAAAGTTTC TAGAATTGGA TGATAAGAAA 
ACAGAAGTAT ATGCCCAAAA TTGGGTTGGA TCAACGTTAT TACCACCAAA TATATCTTTC 
AGAGAATTTC AGAGTTTGGC GAATATTGTG AACAAAGTTC CGAATGAAAA TTCCATTGAA 
AATTTCTTGG ACCTATGTTT GAAGCTCAGC TTTCCAGTTA ATTTGTTTAC ACTACTTCAT 
GTGTCTATGT GGTCGAACAA TAATTTTATT TATTTCATTC AAAGCTATGT TTCAAAAAAT 
GAAAACAAAT TAAACGTGGA TCTGATCACG CTGTTAAAAA CTTCTTTACC AGGGAATCCT 
GAACTGTTTT CCGGTCTGCT CCCATTTTTA AGGCGTAACA AATTTATGGA TATTTTGGAG 
TACTGTATTC ATTCCAATCC AAATTTACTA AATTCCATCC CTGATCTAAA TTCAGATCTC 
CTATTGAAGC TGTTACCACG TAGCAGAGCT TCATATTTTG CAGCAAACAT AAAACTATTC 
AAGTGCTCCG AACAGCTTAC ATTAGTTCGT TGGCTGTTGA AGGGTCAACA ACTTGAACAG 
CTAAATCAAA ATTTTTCAGA AATCGAGAAC GTTTTACAAA ATGCGTCTGA TAGCGAGTTA 
GAAAAATCAG AAATAATCAG AGAACTCCTT CATCTGGCGA TGGCAAATCC CATTGAACCA 
TTATTCAGTG GCCTATTGAA TTTTTGCATA AAGAATAATA TGGCGGATCA TTTGGATGAA 
TTTTGCGGAA ATATGACCAG CGAAGTTCTT TTCAAAATAA GTCCAGAGCT ACTGTTAAAA 
TTACTGACGT ATAAGGAGAA GCCTAATGGG AAGTTACTGG CAGCAGTTAT TGAAAAAATT 
GAAAATGGAG ATGATGATTA TATATTGGAG TTGCTTGAAA AGATTATTAT ACAAAAAGAG 
ATCCAAATTT TGGAAAAATT AAAGGAGCCA CTTTTAGTTT TCTTCTTAAA TCCAGTTAGC 
TCTAATATGC AAAAACACAA AAAGAGTACA AATATGCTCC GTGAATTAGT CTTATTATAT 
CTCACTAAAC CGCTTTCTAG AAGTGCCGCC AAAAAATTTT TTTCCATGTT AATTAGTATA 
TTACCGCCAA ACCCTAACTA TCAAACTATT GATATGGTTA ATCTGTTGAT TGACCTCATC 
AAATCACATA ATCGTAAGTT TAAAGATAAG AGAACCTATA ATGCAACATT AAAGACAATA 
GGAAAGTGGA TACAGGAATC GGGCGTGGTT CATCAAGGGG ACTCAAGTAA GGAAATTGAA 
GCTATACCTG ACACTAAGTC GATGTATATT CCATGCGAAG GGAGTGAAAA TAAACTTTCA 
AACTTACAGA GAAAAGTGGA TTCTCAAGAT ATCCAGGTTC CGGCTACGCA GGGAATGAAA 
GAGCCTCCCT CCTCAATTCA AATATCTTCC CAGATTAGTG CGAAAGATTC AGATTCAATA 
TCGCTTAAAA ATACTGCAAT AATGAATTCT TCACAGCAAG AATCACATGC TAACCGAAGC 
AGATCAATTG ATGATGAAAC ACTAGAGGAA GTGGATAATG AAAGCATTAG AGAAATAGAT 
CAGCAGATGA AGAGTACGCA GTTAGATAAA AACGTGGCCA ATCATAGCAA CATTTGTTCT 
ACTAAAAGCG ATGAGGTGGA TGTTACTGAG CTGCATGAAA GTATTGATAC ACAATCCTCG 
GAAGTGAACG CATACCAACC GATAGAAGTT CTCACTAGCG AACTGAAGGC GGTAACAAAT 
AGATCTATCA AAACGAATCC TGATCATAAC GTTGTTAACA GTGATAATCC TCTAAAACGA 
CCTTCCAAAG AGACGCCTAC CTCTGAAAAT AAAAGATCGA AAGGTCATGA AACGATGGTG 
GACGTTTTAG TTTCTGAGGA ACAAGCGGTG TCGCCTAGCA GTGACGTTAT ATGTACTAAC 
ATCAAGAGTA TAGCCAACGA AGAATCTTCG TTAGCTTTAA GGAATAGCAT AAAAGTGGAA 
ACAAACTGTA ATGAAAATTC CTTGAATGTC ACTTTAGATC TTGACCAGCA AACCATAACA 
AAGGAAGATG GAAAAGGGCA AGTTGAACAT GTTCAAAGAC AGGAGAACCA GGAAAGTATG 
AATAAAATCA ACAGTAAAAG TTTTACGCAA GACAATATTG CACAGTACAA GAGCGTGAAG 
AAAGCCCGTC CTAATAATGA GGGTGAAAAC AACGATTATG CTTGTAATGT TGAACAAGCC 
TCTCCAGTAA GGAATGAGGT GCCAGGAGAC GGCATTCAGA TCCCGAGTGG GACTATACTT 
CTCAATAGTT CAAAGCAGAC AGAAAAATCA AAAGTTGATG ACTTGCGTAG TGATGAAGAT 
GAACATGGAA CGGTTGCGCA AGAGAAACAT CAAGTGGGTG CAATAAACAG TAGAAACAAA 
AATAATGACA GGATGGACAG CACACCCATT CAAGGCACTG AAGAAGAATC TAGGGAAGTT 
GTTATGACCG AAGAAGGTAT CAATGTACGC TTAGAAGATA GTGGAACATG CGAACTTAAT 
AAAAATTTGA AGGGACCGCT TAAAGGAGAT AAGGATGCCA ATATTAATGA CGATTTTGTA 
CCTGTTGAAG AAAATGTCAG AGATGAGGGC TTTTTAAAAT CAATGGAACA TGCGGTATCT 
AAAGAGACAG GTCTAGAAGA ACAACCCGAA GTTGCTGATA TTTCAGTGTT ACCAGAAATT 
CGTATACCCA TTTTCAATTC TTTGAAAATG CAAGGGTCAA AGAGCCAAAT AAAAGAGAAA 
TTGAAAAAGA GATTGCAAAG GAATGAGTTA ATGCCACCAG ATTCACCTCC TCGAATGACA 
GAAAATACAA ATATCAACGC GCAGAATGGC TTAGACACCG TACCAAAAAC AATTGGTGGA 
AAAGAAAAAC ACCATGAAAT TCAATTAGGC CAAGCCCACA CAGAAGCAGA TGGCGAACCT 
TTGTTGGGGG GAGATGGTAA CGAAGATGCT ACATCTAGGG AAGCCACACC TTCATTAAAA 
GTGCACTTTT TTTCAAAAAA GTCGAGAAGG TTAGTGGCCA GATTGAGAGG GTTCACACCG 
GGCGACTTGA ACGGAATATC TGTGGAAGAA AGAAGAAACT TGAGAATTGA ATTATTAGAT 
TTTATGATGA GGCTCGAATA TTACTCAAAC AGGGATAATG ATATGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009834.4
CDS	1..5751
Translation	MSKDFSDKKKHTIDRIDQHILRRSQHDNYSNGSSPWMKTNLPPPSPQAHMHIQSDLSPTPKRRKLASSSDCENKQFDLSAINKNLYPEDTGSRLMQSLPELSASNSDNVSPVTKSVAFSDRIESSPIYRIPGSSPKPSPSSKPGKSILRNRLPSVRTVSDLSYNKLQYTQHKLHNGNIFTSPYKETRVNPRALEYWVSGEIHGLVDNESVSEFKEIIEGGLGILRQESEDYVARRFEVYATFNNIIPILTTKNVNEVDQKFNILIVNIESIIEICIPHLQIAQDTLLSSSEKKNPFVIRLYVQIVRFFSAIMSNFKIVKWLTKRPDLVNKLKVIYRWTTGALRNENSNKIIITAQVSFLRDEKFGTFFLSNEEIKPIISTFTEIMEINSHNLIYEKLLLIRGFLSKYPKLMIETVTSWLPGEVLPRIIIGDEIYSMKILITSIVVLLELLKKCLDFVDEHERIYQCIMLSPVCETIPEKFLSKLPLNSYDSANLDKVTIGHLLTQQIKNYIVVKNDNKIAMDLWLSMTGLLYDSGKRVYDLTSESNKVWFDLNNLCFINNHPKTRLMSIKVWRIITYCICTKISQKNQEGNKSLLSLLRTPFQMTLPYVNDPSAREGIIYHLLGVVYTAFTSNKNLSTDMFELFWDHLITPIYEDYVFKYDSIHLQNVLFTVLHLLIGGKNADVALERKYKKHIHPMSVIASEGVKLKDISSLPPQIIKREYDKIMKVVFQAVEVAISNVNLAHDLILTSLKHLPEDRKDQTHLESFSSLILKVTQNNKDTPIFRDFFGAVTSSFVYTFLDLFLRKNDSSLVNFNIQISKVGISQGNMTLDLLKDVIRKARNETSEFLIIEKFLELDDKKTEVYAQNWVGSTLLPPNISFREFQSLANIVNKVPNENSIENFLDLCLKLSFPVNLFTLLHVSMWSNNNFIYFIQSYVSKNENKLNVDLITLLKTSLPGNPELFSGLLPFLRRNKFMDILEYCIHSNPNLLNSIPDLNSDLLLKLLPRSRASYFAANIKLFKCSEQLTLVRWLLKGQQLEQLNQNFSEIENVLQNASDSELEKSEIIRELLHLAMANPIEPLFSGLLNFCIKNNMADHLDEFCGNMTSEVLFKISPELLLKLLTYKEKPNGKLLAAVIEKIENGDDDYILELLEKIIIQKEIQILEKLKEPLLVFFLNPVSSNMQKHKKSTNMLRELVLLYLTKPLSRSAAKKFFSMLISILPPNPNYQTIDMVNLLIDLIKSHNRKFKDKRTYNATLKTIGKWIQESGVVHQGDSSKEIEAIPDTKSMYIPCEGSENKLSNLQRKVDSQDIQVPATQGMKEPPSSIQISSQISAKDSDSISLKNTAIMNSSQQESHANRSRSIDDETLEEVDNESIREIDQQMKSTQLDKNVANHSNICSTKSDEVDVTELHESIDTQSSEVNAYQPIEVLTSELKAVTNRSIKTNPDHNVVNSDNPLKRPSKETPTSENKRSKGHETMVDVLVSEEQAVSPSSDVICTNIKSIANEESSLALRNSIKVETNCNENSLNVTLDLDQQTITKEDGKGQVEHVQRQENQESMNKINSKSFTQDNIAQYKSVKKARPNNEGENNDYACNVEQASPVRNEVPGDGIQIPSGTILLNSSKQTEKSKVDDLRSDEDEHGTVAQEKHQVGAINSRNKNNDRMDSTPIQGTEEESREVVMTEEGINVRLEDSGTCELNKNLKGPLKGDKDANINDDFVPVEENVRDEGFLKSMEHAVSKETGLEEQPEVADISVLPEIRIPIFNSLKMQGSKSQIKEKLKKRLQRNELMPPDSPPRMTENTNINAQNGLDTVPKTIGGKEKHHEIQLGQAHTEADGEPLLGGDGNEDATSREATPSLKVHFFSKKSRRLVARLRGFTPGDLNGISVEERRNLRIELLDFMMRLEYYSNRDNDMN

Target ORF information:

RefSeq Version	NM_001178623.4
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-binding protein RIF1 (RIF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178623.4

ORF Insert Sequence:

ATGTCGAAAG ATTTTTCAGA TAAAAAGAAA CATACGATAG ATCGAATTGA TCAGCATATC 
CTCCGTAGGT CTCAACACGA TAATTATTCC AATGGAAGCT CTCCGTGGAT GAAAACTAAC 
TTGCCACCTC CATCTCCACA AGCTCATATG CATATACAAA GCGATCTATC GCCAACGCCA 
AAGAGGCGAA AGCTAGCGTC TTCAAGTGAT TGCGAGAATA AACAATTTGA TTTATCAGCT 
ATTAACAAGA ATTTATATCC AGAAGATACA GGAAGCAGGC TAATGCAAAG TCTTCCAGAG 
TTGTCTGCCT CTAATAGTGA TAATGTATCA CCGGTTACAA AAAGTGTAGC TTTTTCTGAT 
AGGATAGAAT CATCACCGAT TTACCGCATA CCAGGTTCCT CACCTAAACC CTCTCCATCA 
AGCAAACCAG GAAAATCAAT TTTGAGGAAT AGGTTGCCTT CAGTGAGGAC TGTAAGCGAC 
TTGAGTTACA ATAAACTCCA ATATACCCAA CACAAATTAC ACAACGGTAA TATTTTCACA 
TCACCTTATA AGGAAACAAG GGTAAATCCT CGCGCCTTAG AGTATTGGGT GAGTGGTGAG 
ATCCATGGTT TAGTTGATAA TGAAAGTGTT TCGGAGTTTA AAGAAATTAT TGAGGGCGGA 
TTGGGCATCT TGCGACAAGA ATCTGAAGAT TACGTCGCGA GGAGGTTTGA GGTATACGCA 
ACCTTTAATA ATATTATACC CATTCTAACC ACTAAAAATG TTAACGAGGT TGATCAGAAA 
TTCAATATTC TAATTGTGAA TATAGAAAGT ATCATTGAAA TATGCATACC GCATCTACAA 
ATAGCACAGG ACACATTATT AAGTAGTAGT GAAAAAAAAA ATCCATTTGT AATCAGACTA 
TATGTTCAAA TAGTTCGATT CTTCAGTGCT ATTATGTCTA ATTTCAAAAT CGTTAAGTGG 
CTAACGAAAA GGCCAGATTT GGTTAATAAG TTAAAAGTTA TTTACAGGTG GACCACCGGT 
GCGCTAAGAA ATGAAAATTC AAACAAGATA ATCATAACAG CACAAGTCTC TTTCTTGAGA 
GACGAAAAAT TTGGTACTTT TTTCTTGAGC AACGAAGAAA TTAAACCTAT CATTAGCACT 
TTCACTGAAA TAATGGAAAT CAATAGTCAC AACCTAATAT ATGAAAAATT GCTACTTATA 
AGGGGATTTT TGAGCAAGTA TCCAAAATTA ATGATTGAGA CGGTAACTTC TTGGTTACCA 
GGTGAGGTAC TACCTAGGAT TATTATCGGA GATGAAATTT ACTCCATGAA AATTCTCATA 
ACTTCAATAG TTGTTTTACT GGAACTACTA AAAAAATGCT TAGATTTTGT TGATGAACAT 
GAAAGGATTT ACCAGTGTAT CATGCTATCT CCAGTATGCG AAACAATCCC GGAAAAATTT 
TTATCTAAAC TACCGTTAAA TTCATATGAC AGCGCTAATT TAGATAAAGT GACAATTGGT 
CATCTTCTTA CACAGCAGAT AAAAAACTAT ATTGTGGTCA AAAATGACAA TAAAATAGCA 
ATGGATTTGT GGCTTTCAAT GACCGGATTA CTATACGATA GCGGAAAGAG AGTTTATGAT 
TTGACATCAG AATCTAACAA AGTTTGGTTT GATTTAAATA ACCTGTGCTT TATCAATAAT 
CACCCCAAGA CTCGCTTAAT GTCCATCAAA GTTTGGAGAA TTATTACCTA CTGCATATGC 
ACGAAAATAT CACAGAAAAA TCAGGAAGGA AACAAAAGTT TACTGTCACT TTTACGAACC 
CCTTTTCAAA TGACGCTTCC TTATGTGAAT GATCCAAGCG CTAGAGAAGG GATCATCTAC 
CACCTTTTAG GTGTTGTTTA CACTGCATTT ACAAGTAATA AGAATTTGTC AACAGATATG 
TTTGAACTAT TTTGGGACCA CCTAATAACC CCGATTTATG AAGATTATGT TTTCAAATAT 
GATTCGATTC ACTTGCAAAA TGTACTCTTC ACAGTTTTGC ATTTGTTAAT TGGGGGAAAG 
AATGCAGATG TTGCATTGGA AAGAAAATAT AAAAAGCATA TTCACCCAAT GAGTGTTATA 
GCCTCAGAAG GTGTAAAGTT AAAAGACATT TCAAGCCTGC CCCCGCAGAT CATCAAGCGT 
GAATACGATA AAATCATGAA AGTTGTGTTC CAGGCTGTTG AAGTAGCCAT TTCTAACGTT 
AATTTGGCTC ATGATCTGAT CCTTACATCT TTGAAGCATC TTCCAGAGGA TAGAAAGGAC 
CAAACTCATT TAGAATCCTT TAGCAGTCTT ATATTAAAAG TCACGCAAAA CAACAAAGAT 
ACACCCATTT TTCGTGACTT TTTTGGTGCT GTTACTTCAT CGTTTGTTTA TACATTTTTG 
GACCTGTTTT TAAGAAAGAA TGATTCATCA TTAGTAAATT TCAATATCCA AATTTCCAAA 
GTTGGTATTT CGCAAGGAAA CATGACCCTT GATCTTTTAA AAGATGTTAT CAGGAAAGCT 
CGAAACGAAA CATCTGAATT TCTGATAATA GAAAAGTTTC TAGAATTGGA TGATAAGAAA 
ACAGAAGTAT ATGCCCAAAA TTGGGTTGGA TCAACGTTAT TACCACCAAA TATATCTTTC 
AGAGAATTTC AGAGTTTGGC GAATATTGTG AACAAAGTTC CGAATGAAAA TTCCATTGAA 
AATTTCTTGG ACCTATGTTT GAAGCTCAGC TTTCCAGTTA ATTTGTTTAC ACTACTTCAT 
GTGTCTATGT GGTCGAACAA TAATTTTATT TATTTCATTC AAAGCTATGT TTCAAAAAAT 
GAAAACAAAT TAAACGTGGA TCTGATCACG CTGTTAAAAA CTTCTTTACC AGGGAATCCT 
GAACTGTTTT CCGGTCTGCT CCCATTTTTA AGGCGTAACA AATTTATGGA TATTTTGGAG 
TACTGTATTC ATTCCAATCC AAATTTACTA AATTCCATCC CTGATCTAAA TTCAGATCTC 
CTATTGAAGC TGTTACCACG TAGCAGAGCT TCATATTTTG CAGCAAACAT AAAACTATTC 
AAGTGCTCCG AACAGCTTAC ATTAGTTCGT TGGCTGTTGA AGGGTCAACA ACTTGAACAG 
CTAAATCAAA ATTTTTCAGA AATCGAGAAC GTTTTACAAA ATGCGTCTGA TAGCGAGTTA 
GAAAAATCAG AAATAATCAG AGAACTCCTT CATCTGGCGA TGGCAAATCC CATTGAACCA 
TTATTCAGTG GCCTATTGAA TTTTTGCATA AAGAATAATA TGGCGGATCA TTTGGATGAA 
TTTTGCGGAA ATATGACCAG CGAAGTTCTT TTCAAAATAA GTCCAGAGCT ACTGTTAAAA 
TTACTGACGT ATAAGGAGAA GCCTAATGGG AAGTTACTGG CAGCAGTTAT TGAAAAAATT 
GAAAATGGAG ATGATGATTA TATATTGGAG TTGCTTGAAA AGATTATTAT ACAAAAAGAG 
ATCCAAATTT TGGAAAAATT AAAGGAGCCA CTTTTAGTTT TCTTCTTAAA TCCAGTTAGC 
TCTAATATGC AAAAACACAA AAAGAGTACA AATATGCTCC GTGAATTAGT CTTATTATAT 
CTCACTAAAC CGCTTTCTAG AAGTGCCGCC AAAAAATTTT TTTCCATGTT AATTAGTATA 
TTACCGCCAA ACCCTAACTA TCAAACTATT GATATGGTTA ATCTGTTGAT TGACCTCATC 
AAATCACATA ATCGTAAGTT TAAAGATAAG AGAACCTATA ATGCAACATT AAAGACAATA 
GGAAAGTGGA TACAGGAATC GGGCGTGGTT CATCAAGGGG ACTCAAGTAA GGAAATTGAA 
GCTATACCTG ACACTAAGTC GATGTATATT CCATGCGAAG GGAGTGAAAA TAAACTTTCA 
AACTTACAGA GAAAAGTGGA TTCTCAAGAT ATCCAGGTTC CGGCTACGCA GGGAATGAAA 
GAGCCTCCCT CCTCAATTCA AATATCTTCC CAGATTAGTG CGAAAGATTC AGATTCAATA 
TCGCTTAAAA ATACTGCAAT AATGAATTCT TCACAGCAAG AATCACATGC TAACCGAAGC 
AGATCAATTG ATGATGAAAC ACTAGAGGAA GTGGATAATG AAAGCATTAG AGAAATAGAT 
CAGCAGATGA AGAGTACGCA GTTAGATAAA AACGTGGCCA ATCATAGCAA CATTTGTTCT 
ACTAAAAGCG ATGAGGTGGA TGTTACTGAG CTGCATGAAA GTATTGATAC ACAATCCTCG 
GAAGTGAACG CATACCAACC GATAGAAGTT CTCACTAGCG AACTGAAGGC GGTAACAAAT 
AGATCTATCA AAACGAATCC TGATCATAAC GTTGTTAACA GTGATAATCC TCTAAAACGA 
CCTTCCAAAG AGACGCCTAC CTCTGAAAAT AAAAGATCGA AAGGTCATGA AACGATGGTG 
GACGTTTTAG TTTCTGAGGA ACAAGCGGTG TCGCCTAGCA GTGACGTTAT ATGTACTAAC 
ATCAAGAGTA TAGCCAACGA AGAATCTTCG TTAGCTTTAA GGAATAGCAT AAAAGTGGAA 
ACAAACTGTA ATGAAAATTC CTTGAATGTC ACTTTAGATC TTGACCAGCA AACCATAACA 
AAGGAAGATG GAAAAGGGCA AGTTGAACAT GTTCAAAGAC AGGAGAACCA GGAAAGTATG 
AATAAAATCA ACAGTAAAAG TTTTACGCAA GACAATATTG CACAGTACAA GAGCGTGAAG 
AAAGCCCGTC CTAATAATGA GGGTGAAAAC AACGATTATG CTTGTAATGT TGAACAAGCC 
TCTCCAGTAA GGAATGAGGT GCCAGGAGAC GGCATTCAGA TCCCGAGTGG GACTATACTT 
CTCAATAGTT CAAAGCAGAC AGAAAAATCA AAAGTTGATG ACTTGCGTAG TGATGAAGAT 
GAACATGGAA CGGTTGCGCA AGAGAAACAT CAAGTGGGTG CAATAAACAG TAGAAACAAA 
AATAATGACA GGATGGACAG CACACCCATT CAAGGCACTG AAGAAGAATC TAGGGAAGTT 
GTTATGACCG AAGAAGGTAT CAATGTACGC TTAGAAGATA GTGGAACATG CGAACTTAAT 
AAAAATTTGA AGGGACCGCT TAAAGGAGAT AAGGATGCCA ATATTAATGA CGATTTTGTA 
CCTGTTGAAG AAAATGTCAG AGATGAGGGC TTTTTAAAAT CAATGGAACA TGCGGTATCT 
AAAGAGACAG GTCTAGAAGA ACAACCCGAA GTTGCTGATA TTTCAGTGTT ACCAGAAATT 
CGTATACCCA TTTTCAATTC TTTGAAAATG CAAGGGTCAA AGAGCCAAAT AAAAGAGAAA 
TTGAAAAAGA GATTGCAAAG GAATGAGTTA ATGCCACCAG ATTCACCTCC TCGAATGACA 
GAAAATACAA ATATCAACGC GCAGAATGGC TTAGACACCG TACCAAAAAC AATTGGTGGA 
AAAGAAAAAC ACCATGAAAT TCAATTAGGC CAAGCCCACA CAGAAGCAGA TGGCGAACCT 
TTGTTGGGGG GAGATGGTAA CGAAGATGCT ACATCTAGGG AAGCCACACC TTCATTAAAA 
GTGCACTTTT TTTCAAAAAA GTCGAGAAGG TTAGTGGCCA GATTGAGAGG GTTCACACCG 
GGCGACTTGA ACGGAATATC TGTGGAAGAA AGAAGAAACT TGAGAATTGA ATTATTAGAT 
TTTATGATGA GGCTCGAATA TTACTCAAAC AGGGATAATG ATATGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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RIF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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