TSC10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC10
Entrez Gene ID	852568
Full Name	3-dehydrosphinganine reductase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178613.2	NP_009824.2	3-dehydrosphinganine reductase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F01749g	XP_455159.1
Eremothecium gossypii	AGOS_AEL164C	NP_984697.1
Saccharomyces cerevisiae (baker's yeast)	TSC10	NP_009824.2
Neurospora crassa	NCU00302	XP_957773.2
Gallus gallus (chicken)	KDSR	NP_001026173.1
Schizosaccharomyces pombe (fission yeast)	SPCC1450.15	NP_588314.2
Magnaporthe oryzae (rice blast fungus)	MGG_00362	XP_003718719.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TSC10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01962	NM_001178613.2 Latest version!	Saccharomyces cerevisiae S288C 3-dehydrosphinganine reductase (TSC10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC10 ( NM_001178613.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009824.2 Saccharomyces cerevisiae S288C 3-dehydrosphinganine reductase (TSC10), partial mRNA.

Price & Availability↑

CloneID	OSi01962
Clone ID Related Accession (Same CDS sequence)	NM_001178613.2
Accession Version	NM_001178613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	3-dehydrosphinganine reductase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178613.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGTTTA CGTTAGAAGA CCAAGTTGTG TTGATCACTG GTGGTTCACA AGGTCTTGGA 
AAGGAATTCG CCAAAAAATA TTATAATGAG GCTGAAAACA CAAAGATTAT TATCGTCAGT 
AGGTCAGAGG CTAGACTGCT GGACACATGC AACGAAATTA GGATTGAAGC TCACCTGAGA 
AGGGAAACCA CTGACGAGGG CCAAGTGCAA CATAAGTTGG CTGCGCCCTT GGACCTTGAG 
CAACGGTTAT TTTACTACCC ATGCGACTTG TCCTGCTACG AATCCGTGGA ATGTTTGTTC 
AATGCCCTGA GAGACTTGGA TTTACTCCCT ACACAAACGT TATGCTGTGC AGGGGGGGCT 
GTTCCTAAGT TATTTCGTGG GCTAAGCGGA CATGAGTTGA ACTTGGGTAT GGACATCAAC 
TATAAAACAA CTTTGAACGT GGCACATCAG ATTGCCCTTG CAGAGCAAAC CAAGGAACAC 
CACCTCATCA TCTTTTCTAG TGCCACCGCG CTTTACCCAT TTGTGGGCTA TTCCCAGTAT 
GCGCCTGCAA AAGCTGCAAT CAAATCACTG GTAGCAATCT TAAGACAAGA ACTGACGAAC 
TTCCGTATCA GTTGTGTTTA TCCTGGTAAT TTTGAAAGCG AAGGTTTCAC TGTAGAGCAG 
CTAACGAAAC CCGAAATTAC AAAGTTGATC GAAGGCCCCT CAGACGCTAT CCCATGCAAA 
CAAGCATGTG ATATCATTGC CAAGTCGCTG GCCAGAGGTG ATGATGACGT TTTTACAGAT 
TTTGTCGGAT GGATGATAAT GGGGATGGAC CTTGGGCTCA CCGCAAAGAA AAGCCGCTTT 
GTTCCGTTGC AATGGATTTT TGGTGTCCTA TCAAACATTC TGGTCGTGCC ATTCTACATG 
GTTGGCTGTT CCTGGTATAT CAGGAAATGG TTTCGTGAAA ATGACGGCAA GAAGGCCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009824.2
CDS	1..963
Translation	MKFTLEDQVVLITGGSQGLGKEFAKKYYNEAENTKIIIVSRSEARLLDTCNEIRIEAHLRRETTDEGQVQHKLAAPLDLEQRLFYYPCDLSCYESVECLFNALRDLDLLPTQTLCCAGGAVPKLFRGLSGHELNLGMDINYKTTLNVAHQIALAEQTKEHHLIIFSSATALYPFVGYSQYAPAKAAIKSLVAILRQELTNFRISCVYPGNFESEGFTVEQLTKPEITKLIEGPSDAIPCKQACDIIAKSLARGDDDVFTDFVGWMIMGMDLGLTAKKSRFVPLQWIFGVLSNILVVPFYMVGCSWYIRKWFRENDGKKAN

Target ORF information:

RefSeq Version	NM_001178613.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 3-dehydrosphinganine reductase (TSC10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178613.2

ORF Insert Sequence:

ATGAAGTTTA CGTTAGAAGA CCAAGTTGTG TTGATCACTG GTGGTTCACA AGGTCTTGGA 
AAGGAATTCG CCAAAAAATA TTATAATGAG GCTGAAAACA CAAAGATTAT TATCGTCAGT 
AGGTCAGAGG CTAGACTGCT GGACACATGC AACGAAATTA GGATTGAAGC TCACCTGAGA 
AGGGAAACCA CTGACGAGGG CCAAGTGCAA CATAAGTTGG CTGCGCCCTT GGACCTTGAG 
CAACGGTTAT TTTACTACCC ATGCGACTTG TCCTGCTACG AATCCGTGGA ATGTTTGTTC 
AATGCCCTGA GAGACTTGGA TTTACTCCCT ACACAAACGT TATGCTGTGC AGGGGGGGCT 
GTTCCTAAGT TATTTCGTGG GCTAAGCGGA CATGAGTTGA ACTTGGGTAT GGACATCAAC 
TATAAAACAA CTTTGAACGT GGCACATCAG ATTGCCCTTG CAGAGCAAAC CAAGGAACAC 
CACCTCATCA TCTTTTCTAG TGCCACCGCG CTTTACCCAT TTGTGGGCTA TTCCCAGTAT 
GCGCCTGCAA AAGCTGCAAT CAAATCACTG GTAGCAATCT TAAGACAAGA ACTGACGAAC 
TTCCGTATCA GTTGTGTTTA TCCTGGTAAT TTTGAAAGCG AAGGTTTCAC TGTAGAGCAG 
CTAACGAAAC CCGAAATTAC AAAGTTGATC GAAGGCCCCT CAGACGCTAT CCCATGCAAA 
CAAGCATGTG ATATCATTGC CAAGTCGCTG GCCAGAGGTG ATGATGACGT TTTTACAGAT 
TTTGTCGGAT GGATGATAAT GGGGATGGAC CTTGGGCTCA CCGCAAAGAA AAGCCGCTTT 
GTTCCGTTGC AATGGATTTT TGGTGTCCTA TCAAACATTC TGGTCGTGCC ATTCTACATG 
GTTGGCTGTT CCTGGTATAT CAGGAAATGG TTTCGTGAAA ATGACGGCAA GAAGGCCAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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TSC10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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