PRP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP5
Entrez Gene ID	852539
Full Name	DEAD-box RNA helicase PRP5
Synonyms	RNA5
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178585.1	NP_009796.1	DEAD-box RNA helicase PRP5

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PRP5	NP_009796.1
Eremothecium gossypii	AGOS_AFL027C	NP_985521.2
Kluyveromyces lactis	KLLA0F10505g	XP_455558.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

different HSH155 alleles elicit disparate effects on splicing: some increase the fidelity of BS selection while others decrease fidelity. Our data support a model wherein conformational changes in SF3b1 promote U2 association with the BS independently of the action of the DEAD-box ATPase Prp5. We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing
Title: SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.

Taken together, our results indicate that the Psis in U2 snRNA contribute to pre-mRNA splicing by directly altering the binding/ATPase activity of Prp5.
Title: Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly.

Prp5 binds to the spliceosome in association with U2 by interacting with the BSL and is released upon the base-pairing of U2 with the branch site to allow the recruitment of the tri-snRNP.
Title: A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.

The following PRP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01933	NM_001178585.1 Latest version!	Saccharomyces cerevisiae S288C DEAD-box RNA helicase PRP5 (PRP5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP5 ( NM_001178585.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009796.1 Saccharomyces cerevisiae S288C DEAD-box RNA helicase PRP5 (PRP5), partial mRNA.

Price & Availability↑

CloneID	OSi01933
Clone ID Related Accession (Same CDS sequence)	NM_001178585.1
Accession Version	NM_001178585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DEAD-box RNA helicase PRP5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAACTA TTGATTCGAA GCAAAATATT AATAGGGAGT CTTTATTGGA GGAAAGGAGG 
AAAAAACTAG CAAAATGGAA ACAAAAAAAA GCACAATTTG ATGCTCAGAA AGAGCATCAA 
ACTTCACGCA ATGACATTGT TACTAACAGT TTAGAAGGTA AGCAGACTAC TGAAAAGTTT 
ACGGAAAGAC AGGAACGTGT AAAAGAAGAG CTTCGGAAGA GAAAAAATGA ATTTAGGAAA 
TCTGACGAAC CTGTATCGGT TAAGCCTTCG AAGAAAAAGT CGAAGAGAAG TAAGGTAAAG 
AAGAAAATAT CCTTCGACTT CAGTGATGAC GATGATTCTG AAATAGGAGT TTCTTTTAGA 
TCGAAGGAGC ACATTCAAAA AGCTCCAGAG CATGATAATG AAAAAGATCC ACTGGATGAG 
TTTATGACGT CATTGAAGGA GGAGAAAATG AGTAATAGTA AAGGCATGTA CGACAGAGGC 
GACATTCTTG ATGTAGAGGA TCAATTGTTT GAACTTGGAG GAACCGACGA TGAGGATGTT 
GAAGATAATA CGGATAATTC TAATATAGCG AAAATTGCTA AACTCAAAGC AAAAAAACGT 
GTAAAGCAAA TTTATTACTC TCCAGAAGAG CTTGAGCCGT TCCAAAAAAA TTTTTATATA 
GAATCTGAAA CAGTTTCCTC GATGTCAGAG ATGGAAGTTG AGGAGCTTAG ACTTAGTCTG 
GATAATATCA AAATAAAGGG AACAGGTTGC CCCAAACCAG TCACAAAGTG GTCTCAACTG 
GGACTATCAA CGGACACCAT GGTTTTAATT ACAGAAAAGT TGCACTTTGG CTCCTTAACG 
CCTATTCAAT CCCAGGCTCT TCCCGCTATT ATGTCAGGTC GTGATGTTAT AGGAATATCA 
AAAACTGGCT CCGGCAAGAC CATCTCCTAT CTTTTACCAT TGCTAAGACA AGTAAAAGCT 
CAACGGCCAT TATCAAAACA TGAAACAGGG CCCATGGGTT TAATTCTGGC TCCAACTAGA 
GAGCTAGCTT TACAAATACA TGAAGAAGTT ACTAAATTCA CAGAAGCAGA TACATCCATT 
AGATCAGTAT GCTGTACAGG AGGTTCTGAA ATGAAAAAGC AGATTACTGA TCTTAAAAGA 
GGCACTGAGA TTGTTGTTGC CACACCGGGA CGATTTATTG ATATATTAAC ACTAAATGAT 
GGGAAATTAC TTAGTACTAA AAGAATAACG TTCGTAGTAA TGGATGAGGC AGACAGGCTG 
TTCGATTTAG GTTTTGAACC TCAAATAACG CAAATCATGA AAACTGTTCG ACCGGATAAA 
CAGTGTGTTC TATTTAGTGC AACTTTTCCG AACAAACTAC GCAGTTTTGC CGTAAGAGTT 
TTGCATTCCC CAATATCTAT TACGATTAAT TCAAAGGGAA TGGTTAATGA AAACGTAAAA 
CAAAAGTTTA GAATATGCCA TTCAGAAGAT GAAAAATTTG ACAATCTGGT GCAGCTTATC 
CATGAGCGCA GTGAATTTTT TGACGAGGTT CAAAGTGAAA ATGACGGACA GTCAAGCGAT 
GTTGAAGAAG TTGATGCTAA AGCCATTATA TTTGTATCAA GTCAGAATAT TTGCGACTTC 
ATTTCAAAAA AGCTGTTAAA TGCTGGAATT GTGACCTGTG CTATTCATGC AGGTAAGCCA 
TATCAAGAAA GGCTTATGAA CTTAGAAAAG TTCAAACGAG AAAAGAACAG TATTCTTCTC 
TGTACAGAGG TTCTCTCAAG AGGTTTAAAT GTTCCCGAAG TGTCGTTGGT TATTATCTAT 
AACGCCGTCA AAACTTTCGC GCAATATGTT CATACTACTG GAAGAACTGC CAGAGGTAGT 
CGCTCCGGTA CTGCTATTAC TCTTCTATTG CATGATGAAC TGTCAGGTGC TTATATCTTG 
AGCAAGGCAA TGCGCGATGA AGAAATAAAA GCTTTAGACC CTCTGCAAGC AAAAGAACTT 
CAGGAAATGA GTGCCAAGTT TGAATCCGGT ATGAAAAAGG GTAAGTTTAG GTTGTCGAAA 
GGGTTCGGCG GCAAAGGACT GGAAAATATC AAAAGTAAAA GAGAGGAAGC TCAAAATAAA 
GATTTGGAGC TCAAAAAGAA TGATAAGAGG AGCGATGACT TGGAAAAGAA AATCAGCAAT 
CCACGCGAAG GGCATGACAG TGTGTCTGAG TCTTCTGCGC TTATTCCGAG ACTCAATTAT 
GAACTATTCA AAGAGTCTAC AGACGGGTCG ATCATCTTTT ACGCCAAGGT TTATATTAAT 
GATTTACCCC AGATTGTAAG ATGGGAGGCT ACAAAGAATA CAACCTTATT ATTTATCAAG 
CATGAAACCG GATGTAGTAT TACTAACAAG GGTAAATTTT ACCCTGAAGG GAAGGAACCA 
AAGAATGAAA ACGATGAGCC TAAACTATAC TTATTGATCG AAGGCCAAGA TGAGAAAGAC 
ATACAATTAA GTATAGAATT GCTGGAGCAA AAAGTTAAAG AGGGGGTCGT AAAGGCTGCA 
AGCTTGTCTT TGAAGAGTAC TAAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009796.1
CDS	1..2550
Translation	METIDSKQNINRESLLEERRKKLAKWKQKKAQFDAQKEHQTSRNDIVTNSLEGKQTTEKFTERQERVKEELRKRKNEFRKSDEPVSVKPSKKKSKRSKVKKKISFDFSDDDDSEIGVSFRSKEHIQKAPEHDNEKDPLDEFMTSLKEEKMSNSKGMYDRGDILDVEDQLFELGGTDDEDVEDNTDNSNIAKIAKLKAKKRVKQIYYSPEELEPFQKNFYIESETVSSMSEMEVEELRLSLDNIKIKGTGCPKPVTKWSQLGLSTDTMVLITEKLHFGSLTPIQSQALPAIMSGRDVIGISKTGSGKTISYLLPLLRQVKAQRPLSKHETGPMGLILAPTRELALQIHEEVTKFTEADTSIRSVCCTGGSEMKKQITDLKRGTEIVVATPGRFIDILTLNDGKLLSTKRITFVVMDEADRLFDLGFEPQITQIMKTVRPDKQCVLFSATFPNKLRSFAVRVLHSPISITINSKGMVNENVKQKFRICHSEDEKFDNLVQLIHERSEFFDEVQSENDGQSSDVEEVDAKAIIFVSSQNICDFISKKLLNAGIVTCAIHAGKPYQERLMNLEKFKREKNSILLCTEVLSRGLNVPEVSLVIIYNAVKTFAQYVHTTGRTARGSRSGTAITLLLHDELSGAYILSKAMRDEEIKALDPLQAKELQEMSAKFESGMKKGKFRLSKGFGGKGLENIKSKREEAQNKDLELKKNDKRSDDLEKKISNPREGHDSVSESSALIPRLNYELFKESTDGSIIFYAKVYINDLPQIVRWEATKNTTLLFIKHETGCSITNKGKFYPEGKEPKNENDEPKLYLLIEGQDEKDIQLSIELLEQKVKEGVVKAASLSLKSTKY

Target ORF information:

RefSeq Version	NM_001178585.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DEAD-box RNA helicase PRP5 (PRP5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178585.1

ORF Insert Sequence:

ATGGAAACTA TTGATTCGAA GCAAAATATT AATAGGGAGT CTTTATTGGA GGAAAGGAGG 
AAAAAACTAG CAAAATGGAA ACAAAAAAAA GCACAATTTG ATGCTCAGAA AGAGCATCAA 
ACTTCACGCA ATGACATTGT TACTAACAGT TTAGAAGGTA AGCAGACTAC TGAAAAGTTT 
ACGGAAAGAC AGGAACGTGT AAAAGAAGAG CTTCGGAAGA GAAAAAATGA ATTTAGGAAA 
TCTGACGAAC CTGTATCGGT TAAGCCTTCG AAGAAAAAGT CGAAGAGAAG TAAGGTAAAG 
AAGAAAATAT CCTTCGACTT CAGTGATGAC GATGATTCTG AAATAGGAGT TTCTTTTAGA 
TCGAAGGAGC ACATTCAAAA AGCTCCAGAG CATGATAATG AAAAAGATCC ACTGGATGAG 
TTTATGACGT CATTGAAGGA GGAGAAAATG AGTAATAGTA AAGGCATGTA CGACAGAGGC 
GACATTCTTG ATGTAGAGGA TCAATTGTTT GAACTTGGAG GAACCGACGA TGAGGATGTT 
GAAGATAATA CGGATAATTC TAATATAGCG AAAATTGCTA AACTCAAAGC AAAAAAACGT 
GTAAAGCAAA TTTATTACTC TCCAGAAGAG CTTGAGCCGT TCCAAAAAAA TTTTTATATA 
GAATCTGAAA CAGTTTCCTC GATGTCAGAG ATGGAAGTTG AGGAGCTTAG ACTTAGTCTG 
GATAATATCA AAATAAAGGG AACAGGTTGC CCCAAACCAG TCACAAAGTG GTCTCAACTG 
GGACTATCAA CGGACACCAT GGTTTTAATT ACAGAAAAGT TGCACTTTGG CTCCTTAACG 
CCTATTCAAT CCCAGGCTCT TCCCGCTATT ATGTCAGGTC GTGATGTTAT AGGAATATCA 
AAAACTGGCT CCGGCAAGAC CATCTCCTAT CTTTTACCAT TGCTAAGACA AGTAAAAGCT 
CAACGGCCAT TATCAAAACA TGAAACAGGG CCCATGGGTT TAATTCTGGC TCCAACTAGA 
GAGCTAGCTT TACAAATACA TGAAGAAGTT ACTAAATTCA CAGAAGCAGA TACATCCATT 
AGATCAGTAT GCTGTACAGG AGGTTCTGAA ATGAAAAAGC AGATTACTGA TCTTAAAAGA 
GGCACTGAGA TTGTTGTTGC CACACCGGGA CGATTTATTG ATATATTAAC ACTAAATGAT 
GGGAAATTAC TTAGTACTAA AAGAATAACG TTCGTAGTAA TGGATGAGGC AGACAGGCTG 
TTCGATTTAG GTTTTGAACC TCAAATAACG CAAATCATGA AAACTGTTCG ACCGGATAAA 
CAGTGTGTTC TATTTAGTGC AACTTTTCCG AACAAACTAC GCAGTTTTGC CGTAAGAGTT 
TTGCATTCCC CAATATCTAT TACGATTAAT TCAAAGGGAA TGGTTAATGA AAACGTAAAA 
CAAAAGTTTA GAATATGCCA TTCAGAAGAT GAAAAATTTG ACAATCTGGT GCAGCTTATC 
CATGAGCGCA GTGAATTTTT TGACGAGGTT CAAAGTGAAA ATGACGGACA GTCAAGCGAT 
GTTGAAGAAG TTGATGCTAA AGCCATTATA TTTGTATCAA GTCAGAATAT TTGCGACTTC 
ATTTCAAAAA AGCTGTTAAA TGCTGGAATT GTGACCTGTG CTATTCATGC AGGTAAGCCA 
TATCAAGAAA GGCTTATGAA CTTAGAAAAG TTCAAACGAG AAAAGAACAG TATTCTTCTC 
TGTACAGAGG TTCTCTCAAG AGGTTTAAAT GTTCCCGAAG TGTCGTTGGT TATTATCTAT 
AACGCCGTCA AAACTTTCGC GCAATATGTT CATACTACTG GAAGAACTGC CAGAGGTAGT 
CGCTCCGGTA CTGCTATTAC TCTTCTATTG CATGATGAAC TGTCAGGTGC TTATATCTTG 
AGCAAGGCAA TGCGCGATGA AGAAATAAAA GCTTTAGACC CTCTGCAAGC AAAAGAACTT 
CAGGAAATGA GTGCCAAGTT TGAATCCGGT ATGAAAAAGG GTAAGTTTAG GTTGTCGAAA 
GGGTTCGGCG GCAAAGGACT GGAAAATATC AAAAGTAAAA GAGAGGAAGC TCAAAATAAA 
GATTTGGAGC TCAAAAAGAA TGATAAGAGG AGCGATGACT TGGAAAAGAA AATCAGCAAT 
CCACGCGAAG GGCATGACAG TGTGTCTGAG TCTTCTGCGC TTATTCCGAG ACTCAATTAT 
GAACTATTCA AAGAGTCTAC AGACGGGTCG ATCATCTTTT ACGCCAAGGT TTATATTAAT 
GATTTACCCC AGATTGTAAG ATGGGAGGCT ACAAAGAATA CAACCTTATT ATTTATCAAG 
CATGAAACCG GATGTAGTAT TACTAACAAG GGTAAATTTT ACCCTGAAGG GAAGGAACCA 
AAGAATGAAA ACGATGAGCC TAAACTATAC TTATTGATCG AAGGCCAAGA TGAGAAAGAC 
ATACAATTAA GTATAGAATT GCTGGAGCAA AAAGTTAAAG AGGGGGTCGT AAAGGCTGCA 
AGCTTGTCTT TGAAGAGTAC TAAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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PRP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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