DUR1,2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DUR1,2
Entrez Gene ID	852507
Full Name	bifunctional urea carboxylase/allophanate hydrolase
Synonyms	DUR80
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178556.1	NP_009767.1	bifunctional urea carboxylase/allophanate hydrolase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADR051C	NP_984147.2
Saccharomyces cerevisiae (baker's yeast)	DUR1,2	NP_009767.1
Kluyveromyces lactis	KLLA0E08119g	XP_454317.1
Magnaporthe oryzae (rice blast fungus)	MGG_15535	XP_003713501.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DUR1,2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DUR1,2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01907	NM_001178556.1 Latest version!	Saccharomyces cerevisiae S288C bifunctional urea carboxylase/allophanate hydrolase (DUR1,2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DUR1,2 ( NM_001178556.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009767.1 Saccharomyces cerevisiae S288C bifunctional urea carboxylase/allophanate hydrolase (DUR1,2), partial mRNA.

Price & Availability↑

CloneID	OSi01907
Clone ID Related Accession (Same CDS sequence)	NM_001178556.1
Accession Version	NM_001178556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	bifunctional urea carboxylase/allophanate hydrolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACAGTTA GTTCCGATAC AACTGCTGAA ATATCGTTAG GTTGGTCAAT CCAAGACTGG 
ATTGATTTCC ACAAGTCATC AAGCTCCCAG GCTTCACTAA GGCTTCTTGA ATCACTACTA 
GACTCTCAAA ATGTTGCGCC AGTCGATAAT GCGTGGATAT CGCTAATTTC AAAGGAAAAT 
TTACTGCACC AATTCCAAAT TTTAAAGAGC AGAGAAAATA AAGAAACTCT ACCTCTCTAC 
GGTGTCCCTA TTGCTGTTAA GGACAACATC GACGTTAGAG GTCTACCCAC CACCGCTGCA 
TGTCCATCCT TTGCATATGA GCCTTCCAAA GACTCTAAAG TAGTAGAACT ACTAAGAAAT 
GCAGGTGCGA TAATCGTGGG TAAGACAAAC TTGGACCAAT TTGCCACAGG ATTAGTCGGC 
ACACGGTCTC CATATGGGAA AACACCTTGC GCTTTTAGCA AAGAGCATGT ATCTGGTGGT 
TCCTCCGCTG GGTCAGCATC GGTGGTCGCC AGAGGTATCG TACCAATTGC ATTGGGTACT 
GATACAGCAG GTTCTGGTAG AGTCCCAGCC GCCTTGAACA ACCTGATTGG CCTAAAGCCA 
ACAAAGGGCG TCTTTTCCTG TCAAGGTGTA GTTCCCGCTT GTAAATCTTT AGACTGCGTC 
TCCATCTTTG CATTAAACCT AAGTGATGCT GAACGCTGCT TCCGCATCAT GTGCCAGCCA 
GATCCTGATA ATGATGAATA TTCTAGACCC TATGTTTCCA ACCCTTTGAA AAAATTTTCA 
AGCAATGTAA CGATTGCTAT TCCTAAAAAT ATCCCATGGT ATGGTGAAAC CAAGAATCCT 
GTACTGTTTT CCAATGCTGT CGAAAATCTA TCAAGAACGG GCGCTAACGT CATAGAAATT 
GATTTTGAGC CTCTTTTAGA GTTAGCTCGC TGTTTATACG AAGGTACTTG GGTGGCCGAG 
CGTTATCAAG CTATTCAATC GTTTTTGGAC AGTAAACCAC CAAAGGAATC TTTGGACCCT 
ACTGTTATTT CAATTATAGA AGGGGCCAAG AAATACAGTG CAGTAGACTG CTTCAGTTTT 
GAATACAAAA GACAAGGCAT CTTGCAAAAA GTGAGACGAC TTCTCGAATC AGTCGATGTA 
TTGTGTGTGC CCACATGTCC TTTAAATCCT ACTATGCAAC AAGTTGCGGA TGAACCAGTC 
CTAGTCAATT CAAGACAAGG CACATGGACT AATTTTGTCA ACTTGGCAGA TTTGGCAGCC 
CTTGCTGTTC CCGCAGGGTT CCGAGACGAT GGTTTGCCAA ATGGTATTAC TTTAATCGGT 
AAAAAATTCA CAGATTACGC ACTATTAGAG TTGGCTAACC GCTATTTCCA AAATATATTC 
CCCAACGGTT CCAGAACATA CGGTACTTTT ACCTCTTCTT CAGTAAAGCC AGCAAACGAT 
CAATTAGTGG GACCAGACTA TGACCCATCT ACGTCCATAA AATTGGCTGT TGTCGGTGCA 
CATCTTAAGG GTCTGCCTCT ACATTGGCAA TTGGAAAAGG TCAATGCAAC ATATTTATGT 
ACAACAAAAA CATCAAAAGC TTACCAGCTT TTTGCTTTGC CCAAAAATGG ACCAGTTTTA 
AAACCTGGTT TGAGAAGAGT TCAAGATAGC AATGGCTCTC AAATCGAATT AGAAGTGTAC 
AGTGTTCCAA AAGAACTGTT CGGTGCTTTT ATTTCCATGG TTCCTGAACC ATTAGGAATA 
GGTTCAGTGG AGTTAGAATC TGGTGAATGG ATCAAATCCT TTATTTGTGA AGAATCTGGT 
TACAAAGCCA AAGGTACAGT TGATATCACA AAGTATGGTG GATTTAGAGC ATATTTTGAA 
ATGTTGAAGA AAAAAGAGTC CCAAAAGAAG AAGTTATTTG ATACCGTGTT AATTGCCAAT 
AGAGGTGAAA TTGCCGTTCG TATTATCAAG ACATTAAAAA AATTGGGTAT TAGATCAGTT 
GCAGTTTATT CCGACCCTGA TAAATATTCT CAACACGTTA CTGATGCAGA TGTTTCTGTA 
CCCCTTCATG GCACAACCGC AGCCCAAACT TATTTAGACA TGAATAAGAT CATAGATGCC 
GCTAAGCAAA CTAATGCACA GGCCATTATT CCTGGTTATG GTTTCTTGTC GGAAAATGCG 
GATTTTTCTG ATGCGTGCAC CAGTGCTGGC ATTACCTTTG TTGGTCCTTC GGGAGATATT 
ATCAGAGGTT TAGGGTTAAA ACATTCTGCT AGACAGATTG CACAGAAGGC TGGCGTTCCT 
CTAGTGCCAG GCTCTTTGCT TATCACATCA GTTGAAGAGG CTAAGAAAGT CGCAGCGGAA 
TTGGAATACC CAGTTATGGT GAAGTCAACT GCTGGTGGCG GTGGTATTGG TTTGCAGAAA 
GTCGATTCTG AAGAGGACAT CGAGCATATT TTTGAGACTG TGAAACATCA AGGTGAAACA 
TTTTTCGGTG ACGCTGGTGT ATTTCTGGAA CGGTTTATCG AAAATGCCAG GCATGTTGAA 
GTCCAACTTA TGGGAGATGG TTTTGGTAAG GCCATTGCTT TGGGCGAACG TGATTGTTCT 
TTACAGCGTC GTAACCAAAA AGTTATCGAA GAAACTCCTG CACCAAATTT GCCAGAAAAG 
ACGAGGTTGG CGTTAAGAAA GGCAGCTGAA AGTTTGGGAT CTTTATTGAA TTACAAGTGT 
GCTGGTACGG TTGAATTTAT TTACGATGAG AAAAAGGACG AGTTTTACTT TTTAGAAGTT 
AATACAAGAT TACAAGTTGA ACATCCAATA ACAGAAATGG TTACAGGGTT AGACTTGGTC 
GAGTGGATGA TCAGGATTGC CGCTAATGAT GCACCTGATT TTGATTCTAC AAAGGTAGAA 
GTCAATGGGG TTTCAATGGA GGCACGTTTA TATGCTGAAA ATCCATTGAA AAATTTCAGA 
CCTTCTCCAG GTTTACTTGT CGATGTGAAA TTTCCTGATT GGGCAAGAGT GGATACTTGG 
GTTAAGAAAG GTACTAATAT TTCTCCCGAA TATGATCCAA CATTGGCCAA AATTATCGTT 
CATGGGAAAG ACCGTGATGA TGCAATTTCC AAGTTAAATC AAGCGTTAGA AGAAACAAAA 
GTTTACGGAT GTATTACTAA CATTGACTAC CTGAAGTCTA TCATTACCAG TGATTTCTTT 
GCTAAAGCAA AAGTTTCTAC AAACATTTTG AACTCTTATC AATATGAGCC TACCGCCATC 
GAAATTACTT TGCCCGGTGC ACACACTAGT ATTCAGGATT ACCCCGGTAG AGTTGGGTAC 
TGGAGAATTG GTGTTCCGCC CTCTGGTCCA ATGGACGCAT ATTCGTTTAG ATTGGCGAAC 
AGAATTGTTG GTAATGACTA CAGGACTCCT GCCATTGAAG TAACGTTGAC TGGTCCATCC 
ATCGTTTTCC ATTGTGAAAC TGTCATTGCC ATTACTGGTG GTACCGCTCT ATGTACATTA 
GACGGCCAAG AAATTCCCCA ACACAAACCG GTCGAAGTTA AGAGGGGATC TACTTTATCC 
ATTGGCAAGT TGACAAGCGG CTGTAGAGCA TACTTAGGTA TCAGGGGTGG CATTGATGTG 
CCTAAATACT TGGGCTCTTA TTCTACTTTC ACTCTAGGAA ATGTCGGTGG ATACAATGGA 
AGGGTGCTAA AACTTGGAGA CGTACTATTC TTACCAAGCA ATGAAGAAAA TAAATCAGTT 
GAGTGCCTTC CACAGAATAT TCCTCAATCA TTAATTCCTC AAATTTCCGA AACTAAGGAA 
TGGAGAATTG GTGTAACATG TGGTCCCCAT GGGTCTCCAG ATTTTTTTAA ACCTGAGTCC 
ATCGAAGAAT TTTTCAGTGA GAAGTGGAAG GTTCATTACA ACTCCAATAG ATTTGGTGTC 
CGTTTGATTG GACCTAAACC TAAGTGGGCA AGAAGTAATG GTGGTGAAGG TGGTATGCAT 
CCTTCAAACA CTCACGATTA CGTTTATTCT CTGGGTGCAA TTAATTTCAC GGGTGATGAG 
CCAGTTATTA TTACTTGCGA TGGTCCTTCC TTAGGTGGTT TTGTGTGTCA AGCTGTTGTC 
CCAGAAGCAG AACTGTGGAA GGTTGGACAG GTTAAACCCG GTGATTCCAT TCAGTTTGTG 
CCACTTTCTT ACGAAAGCTC GAGATCCTTA AAGGAATCTC AGGATGTTGC AATTAAATCA 
TTGGATGGTA CTAAGTTAAG GCGCTTAGAC TCTGTTTCAA TTTTACCATC ATTCGAAACG 
CCTATTCTTG CACAAATGGA AAAAGTGAAT GAGCTTTCAC CAAAGGTTGT ATACAGACAA 
GCAGGTGATC GTTATGTTTT GGTGGAATAC GGTGATAATG AAATGAATTT TAATATTTCC 
TATAGAATTG AATGCCTGAT CTCCCTTGTG AAAAAGAATA AGACTATTGG TATTGTTGAA 
ATGTCCCAAG GTGTTAGATC TGTATTGATA GAATTTGATG GTTACAAAGT CACTCAAAAA 
GAATTGCTTA AAGTATTGGT GGCATATGAA ACAGAAATCC AGTTTGATGA AAATTGGAAG 
ATAACTTCTA ATATAATAAG ATTACCGATG GCTTTCGAAG ACTCGAAGAC TTTGGCATGT 
GTTCAAAGGT ATCAAGAAAC AATTCGTTCG TCTGCTCCAT GGTTGCCAAA TAACGTTGAT 
TTCATTGCCA ATGTAAATGG AATTTCAAGG AATGAAGTTT ATGATATGTT GTATTCTGCC 
AGATTTATGG TTTTAGGTTT AGGTGATGTC TTCCTAGGGT CGCCTTGTGC TGTTCCATTA 
GATCCTCGTC ACAGATTTTT GGGAAGCAAG TACAACCCAA GTAGAACATA TACAGAAAGA 
GGTGCAGTCG GTATTGGCGG TATGTATATG TGCATATATG CTGCTAACAG TCCTGGTGGG 
TACCAATTAG TGGGTAGAAC AATACCAATT TGGGACAAAC TATGTCTGGC CGCATCTTCT 
GAGGTTCCGT GGTTGATGAA CCCATTTGAC CAAGTCGAAT TTTACCCAGT TTCTGAAGAA 
GATTTGGATA AAATGACTGA AGATTGTGAT AATGGTGTTT ATAAAGTCAA TATCGAAAAG 
AGTGTTTTTG ATCATCAAGA ATACTTGAGA TGGATCAACG CAAACAAAGA TTCCATCACA 
GCATTCCAGG AGGGCCAGCT TGGTGAAAGA GCAGAGGAAT TTGCCAAATT GATTCAAAAT 
GCAAACTCTG AACTAAAAGA AAGTGTCACA GTCAAACCTG ACGAGGAAGA AGACTTCCCA 
GAAGGTGCAG AAATTGTATA TTCTGAGTAT TCTGGGCGTT TTTGGAAATC CATAGCATCT 
GTTGGAGATG TTATTGAAGC AGGTCAAGGG CTACTAATTA TTGAAGCCAT GAAAGCGGAA 
ATGATTATAT CCGCTCCTAA ATCGGGTAAG ATTATCAAGA TTTGCCATGG CAATGGTGAT 
ATGGTTGATT CTGGTGACAT AGTGGCCGTC ATAGAGACAT TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009767.1
CDS	1..5508
Translation	MTVSSDTTAEISLGWSIQDWIDFHKSSSSQASLRLLESLLDSQNVAPVDNAWISLISKENLLHQFQILKSRENKETLPLYGVPIAVKDNIDVRGLPTTAACPSFAYEPSKDSKVVELLRNAGAIIVGKTNLDQFATGLVGTRSPYGKTPCAFSKEHVSGGSSAGSASVVARGIVPIALGTDTAGSGRVPAALNNLIGLKPTKGVFSCQGVVPACKSLDCVSIFALNLSDAERCFRIMCQPDPDNDEYSRPYVSNPLKKFSSNVTIAIPKNIPWYGETKNPVLFSNAVENLSRTGANVIEIDFEPLLELARCLYEGTWVAERYQAIQSFLDSKPPKESLDPTVISIIEGAKKYSAVDCFSFEYKRQGILQKVRRLLESVDVLCVPTCPLNPTMQQVADEPVLVNSRQGTWTNFVNLADLAALAVPAGFRDDGLPNGITLIGKKFTDYALLELANRYFQNIFPNGSRTYGTFTSSSVKPANDQLVGPDYDPSTSIKLAVVGAHLKGLPLHWQLEKVNATYLCTTKTSKAYQLFALPKNGPVLKPGLRRVQDSNGSQIELEVYSVPKELFGAFISMVPEPLGIGSVELESGEWIKSFICEESGYKAKGTVDITKYGGFRAYFEMLKKKESQKKKLFDTVLIANRGEIAVRIIKTLKKLGIRSVAVYSDPDKYSQHVTDADVSVPLHGTTAAQTYLDMNKIIDAAKQTNAQAIIPGYGFLSENADFSDACTSAGITFVGPSGDIIRGLGLKHSARQIAQKAGVPLVPGSLLITSVEEAKKVAAELEYPVMVKSTAGGGGIGLQKVDSEEDIEHIFETVKHQGETFFGDAGVFLERFIENARHVEVQLMGDGFGKAIALGERDCSLQRRNQKVIEETPAPNLPEKTRLALRKAAESLGSLLNYKCAGTVEFIYDEKKDEFYFLEVNTRLQVEHPITEMVTGLDLVEWMIRIAANDAPDFDSTKVEVNGVSMEARLYAENPLKNFRPSPGLLVDVKFPDWARVDTWVKKGTNISPEYDPTLAKIIVHGKDRDDAISKLNQALEETKVYGCITNIDYLKSIITSDFFAKAKVSTNILNSYQYEPTAIEITLPGAHTSIQDYPGRVGYWRIGVPPSGPMDAYSFRLANRIVGNDYRTPAIEVTLTGPSIVFHCETVIAITGGTALCTLDGQEIPQHKPVEVKRGSTLSIGKLTSGCRAYLGIRGGIDVPKYLGSYSTFTLGNVGGYNGRVLKLGDVLFLPSNEENKSVECLPQNIPQSLIPQISETKEWRIGVTCGPHGSPDFFKPESIEEFFSEKWKVHYNSNRFGVRLIGPKPKWARSNGGEGGMHPSNTHDYVYSLGAINFTGDEPVIITCDGPSLGGFVCQAVVPEAELWKVGQVKPGDSIQFVPLSYESSRSLKESQDVAIKSLDGTKLRRLDSVSILPSFETPILAQMEKVNELSPKVVYRQAGDRYVLVEYGDNEMNFNISYRIECLISLVKKNKTIGIVEMSQGVRSVLIEFDGYKVTQKELLKVLVAYETEIQFDENWKITSNIIRLPMAFEDSKTLACVQRYQETIRSSAPWLPNNVDFIANVNGISRNEVYDMLYSARFMVLGLGDVFLGSPCAVPLDPRHRFLGSKYNPSRTYTERGAVGIGGMYMCIYAANSPGGYQLVGRTIPIWDKLCLAASSEVPWLMNPFDQVEFYPVSEEDLDKMTEDCDNGVYKVNIEKSVFDHQEYLRWINANKDSITAFQEGQLGERAEEFAKLIQNANSELKESVTVKPDEEEDFPEGAEIVYSEYSGRFWKSIASVGDVIEAGQGLLIIEAMKAEMIISAPKSGKIIKICHGNGDMVDSGDIVAVIETLA

Target ORF information:

RefSeq Version	NM_001178556.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C bifunctional urea carboxylase/allophanate hydrolase (DUR1,2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178556.1

ORF Insert Sequence:

ATGACAGTTA GTTCCGATAC AACTGCTGAA ATATCGTTAG GTTGGTCAAT CCAAGACTGG 
ATTGATTTCC ACAAGTCATC AAGCTCCCAG GCTTCACTAA GGCTTCTTGA ATCACTACTA 
GACTCTCAAA ATGTTGCGCC AGTCGATAAT GCGTGGATAT CGCTAATTTC AAAGGAAAAT 
TTACTGCACC AATTCCAAAT TTTAAAGAGC AGAGAAAATA AAGAAACTCT ACCTCTCTAC 
GGTGTCCCTA TTGCTGTTAA GGACAACATC GACGTTAGAG GTCTACCCAC CACCGCTGCA 
TGTCCATCCT TTGCATATGA GCCTTCCAAA GACTCTAAAG TAGTAGAACT ACTAAGAAAT 
GCAGGTGCGA TAATCGTGGG TAAGACAAAC TTGGACCAAT TTGCCACAGG ATTAGTCGGC 
ACACGGTCTC CATATGGGAA AACACCTTGC GCTTTTAGCA AAGAGCATGT ATCTGGTGGT 
TCCTCCGCTG GGTCAGCATC GGTGGTCGCC AGAGGTATCG TACCAATTGC ATTGGGTACT 
GATACAGCAG GTTCTGGTAG AGTCCCAGCC GCCTTGAACA ACCTGATTGG CCTAAAGCCA 
ACAAAGGGCG TCTTTTCCTG TCAAGGTGTA GTTCCCGCTT GTAAATCTTT AGACTGCGTC 
TCCATCTTTG CATTAAACCT AAGTGATGCT GAACGCTGCT TCCGCATCAT GTGCCAGCCA 
GATCCTGATA ATGATGAATA TTCTAGACCC TATGTTTCCA ACCCTTTGAA AAAATTTTCA 
AGCAATGTAA CGATTGCTAT TCCTAAAAAT ATCCCATGGT ATGGTGAAAC CAAGAATCCT 
GTACTGTTTT CCAATGCTGT CGAAAATCTA TCAAGAACGG GCGCTAACGT CATAGAAATT 
GATTTTGAGC CTCTTTTAGA GTTAGCTCGC TGTTTATACG AAGGTACTTG GGTGGCCGAG 
CGTTATCAAG CTATTCAATC GTTTTTGGAC AGTAAACCAC CAAAGGAATC TTTGGACCCT 
ACTGTTATTT CAATTATAGA AGGGGCCAAG AAATACAGTG CAGTAGACTG CTTCAGTTTT 
GAATACAAAA GACAAGGCAT CTTGCAAAAA GTGAGACGAC TTCTCGAATC AGTCGATGTA 
TTGTGTGTGC CCACATGTCC TTTAAATCCT ACTATGCAAC AAGTTGCGGA TGAACCAGTC 
CTAGTCAATT CAAGACAAGG CACATGGACT AATTTTGTCA ACTTGGCAGA TTTGGCAGCC 
CTTGCTGTTC CCGCAGGGTT CCGAGACGAT GGTTTGCCAA ATGGTATTAC TTTAATCGGT 
AAAAAATTCA CAGATTACGC ACTATTAGAG TTGGCTAACC GCTATTTCCA AAATATATTC 
CCCAACGGTT CCAGAACATA CGGTACTTTT ACCTCTTCTT CAGTAAAGCC AGCAAACGAT 
CAATTAGTGG GACCAGACTA TGACCCATCT ACGTCCATAA AATTGGCTGT TGTCGGTGCA 
CATCTTAAGG GTCTGCCTCT ACATTGGCAA TTGGAAAAGG TCAATGCAAC ATATTTATGT 
ACAACAAAAA CATCAAAAGC TTACCAGCTT TTTGCTTTGC CCAAAAATGG ACCAGTTTTA 
AAACCTGGTT TGAGAAGAGT TCAAGATAGC AATGGCTCTC AAATCGAATT AGAAGTGTAC 
AGTGTTCCAA AAGAACTGTT CGGTGCTTTT ATTTCCATGG TTCCTGAACC ATTAGGAATA 
GGTTCAGTGG AGTTAGAATC TGGTGAATGG ATCAAATCCT TTATTTGTGA AGAATCTGGT 
TACAAAGCCA AAGGTACAGT TGATATCACA AAGTATGGTG GATTTAGAGC ATATTTTGAA 
ATGTTGAAGA AAAAAGAGTC CCAAAAGAAG AAGTTATTTG ATACCGTGTT AATTGCCAAT 
AGAGGTGAAA TTGCCGTTCG TATTATCAAG ACATTAAAAA AATTGGGTAT TAGATCAGTT 
GCAGTTTATT CCGACCCTGA TAAATATTCT CAACACGTTA CTGATGCAGA TGTTTCTGTA 
CCCCTTCATG GCACAACCGC AGCCCAAACT TATTTAGACA TGAATAAGAT CATAGATGCC 
GCTAAGCAAA CTAATGCACA GGCCATTATT CCTGGTTATG GTTTCTTGTC GGAAAATGCG 
GATTTTTCTG ATGCGTGCAC CAGTGCTGGC ATTACCTTTG TTGGTCCTTC GGGAGATATT 
ATCAGAGGTT TAGGGTTAAA ACATTCTGCT AGACAGATTG CACAGAAGGC TGGCGTTCCT 
CTAGTGCCAG GCTCTTTGCT TATCACATCA GTTGAAGAGG CTAAGAAAGT CGCAGCGGAA 
TTGGAATACC CAGTTATGGT GAAGTCAACT GCTGGTGGCG GTGGTATTGG TTTGCAGAAA 
GTCGATTCTG AAGAGGACAT CGAGCATATT TTTGAGACTG TGAAACATCA AGGTGAAACA 
TTTTTCGGTG ACGCTGGTGT ATTTCTGGAA CGGTTTATCG AAAATGCCAG GCATGTTGAA 
GTCCAACTTA TGGGAGATGG TTTTGGTAAG GCCATTGCTT TGGGCGAACG TGATTGTTCT 
TTACAGCGTC GTAACCAAAA AGTTATCGAA GAAACTCCTG CACCAAATTT GCCAGAAAAG 
ACGAGGTTGG CGTTAAGAAA GGCAGCTGAA AGTTTGGGAT CTTTATTGAA TTACAAGTGT 
GCTGGTACGG TTGAATTTAT TTACGATGAG AAAAAGGACG AGTTTTACTT TTTAGAAGTT 
AATACAAGAT TACAAGTTGA ACATCCAATA ACAGAAATGG TTACAGGGTT AGACTTGGTC 
GAGTGGATGA TCAGGATTGC CGCTAATGAT GCACCTGATT TTGATTCTAC AAAGGTAGAA 
GTCAATGGGG TTTCAATGGA GGCACGTTTA TATGCTGAAA ATCCATTGAA AAATTTCAGA 
CCTTCTCCAG GTTTACTTGT CGATGTGAAA TTTCCTGATT GGGCAAGAGT GGATACTTGG 
GTTAAGAAAG GTACTAATAT TTCTCCCGAA TATGATCCAA CATTGGCCAA AATTATCGTT 
CATGGGAAAG ACCGTGATGA TGCAATTTCC AAGTTAAATC AAGCGTTAGA AGAAACAAAA 
GTTTACGGAT GTATTACTAA CATTGACTAC CTGAAGTCTA TCATTACCAG TGATTTCTTT 
GCTAAAGCAA AAGTTTCTAC AAACATTTTG AACTCTTATC AATATGAGCC TACCGCCATC 
GAAATTACTT TGCCCGGTGC ACACACTAGT ATTCAGGATT ACCCCGGTAG AGTTGGGTAC 
TGGAGAATTG GTGTTCCGCC CTCTGGTCCA ATGGACGCAT ATTCGTTTAG ATTGGCGAAC 
AGAATTGTTG GTAATGACTA CAGGACTCCT GCCATTGAAG TAACGTTGAC TGGTCCATCC 
ATCGTTTTCC ATTGTGAAAC TGTCATTGCC ATTACTGGTG GTACCGCTCT ATGTACATTA 
GACGGCCAAG AAATTCCCCA ACACAAACCG GTCGAAGTTA AGAGGGGATC TACTTTATCC 
ATTGGCAAGT TGACAAGCGG CTGTAGAGCA TACTTAGGTA TCAGGGGTGG CATTGATGTG 
CCTAAATACT TGGGCTCTTA TTCTACTTTC ACTCTAGGAA ATGTCGGTGG ATACAATGGA 
AGGGTGCTAA AACTTGGAGA CGTACTATTC TTACCAAGCA ATGAAGAAAA TAAATCAGTT 
GAGTGCCTTC CACAGAATAT TCCTCAATCA TTAATTCCTC AAATTTCCGA AACTAAGGAA 
TGGAGAATTG GTGTAACATG TGGTCCCCAT GGGTCTCCAG ATTTTTTTAA ACCTGAGTCC 
ATCGAAGAAT TTTTCAGTGA GAAGTGGAAG GTTCATTACA ACTCCAATAG ATTTGGTGTC 
CGTTTGATTG GACCTAAACC TAAGTGGGCA AGAAGTAATG GTGGTGAAGG TGGTATGCAT 
CCTTCAAACA CTCACGATTA CGTTTATTCT CTGGGTGCAA TTAATTTCAC GGGTGATGAG 
CCAGTTATTA TTACTTGCGA TGGTCCTTCC TTAGGTGGTT TTGTGTGTCA AGCTGTTGTC 
CCAGAAGCAG AACTGTGGAA GGTTGGACAG GTTAAACCCG GTGATTCCAT TCAGTTTGTG 
CCACTTTCTT ACGAAAGCTC GAGATCCTTA AAGGAATCTC AGGATGTTGC AATTAAATCA 
TTGGATGGTA CTAAGTTAAG GCGCTTAGAC TCTGTTTCAA TTTTACCATC ATTCGAAACG 
CCTATTCTTG CACAAATGGA AAAAGTGAAT GAGCTTTCAC CAAAGGTTGT ATACAGACAA 
GCAGGTGATC GTTATGTTTT GGTGGAATAC GGTGATAATG AAATGAATTT TAATATTTCC 
TATAGAATTG AATGCCTGAT CTCCCTTGTG AAAAAGAATA AGACTATTGG TATTGTTGAA 
ATGTCCCAAG GTGTTAGATC TGTATTGATA GAATTTGATG GTTACAAAGT CACTCAAAAA 
GAATTGCTTA AAGTATTGGT GGCATATGAA ACAGAAATCC AGTTTGATGA AAATTGGAAG 
ATAACTTCTA ATATAATAAG ATTACCGATG GCTTTCGAAG ACTCGAAGAC TTTGGCATGT 
GTTCAAAGGT ATCAAGAAAC AATTCGTTCG TCTGCTCCAT GGTTGCCAAA TAACGTTGAT 
TTCATTGCCA ATGTAAATGG AATTTCAAGG AATGAAGTTT ATGATATGTT GTATTCTGCC 
AGATTTATGG TTTTAGGTTT AGGTGATGTC TTCCTAGGGT CGCCTTGTGC TGTTCCATTA 
GATCCTCGTC ACAGATTTTT GGGAAGCAAG TACAACCCAA GTAGAACATA TACAGAAAGA 
GGTGCAGTCG GTATTGGCGG TATGTATATG TGCATATATG CTGCTAACAG TCCTGGTGGG 
TACCAATTAG TGGGTAGAAC AATACCAATT TGGGACAAAC TATGTCTGGC CGCATCTTCT 
GAGGTTCCGT GGTTGATGAA CCCATTTGAC CAAGTCGAAT TTTACCCAGT TTCTGAAGAA 
GATTTGGATA AAATGACTGA AGATTGTGAT AATGGTGTTT ATAAAGTCAA TATCGAAAAG 
AGTGTTTTTG ATCATCAAGA ATACTTGAGA TGGATCAACG CAAACAAAGA TTCCATCACA 
GCATTCCAGG AGGGCCAGCT TGGTGAAAGA GCAGAGGAAT TTGCCAAATT GATTCAAAAT 
GCAAACTCTG AACTAAAAGA AAGTGTCACA GTCAAACCTG ACGAGGAAGA AGACTTCCCA 
GAAGGTGCAG AAATTGTATA TTCTGAGTAT TCTGGGCGTT TTTGGAAATC CATAGCATCT 
GTTGGAGATG TTATTGAAGC AGGTCAAGGG CTACTAATTA TTGAAGCCAT GAAAGCGGAA 
ATGATTATAT CCGCTCCTAA ATCGGGTAAG ATTATCAAGA TTTGCCATGG CAATGGTGAT 
ATGGTTGATT CTGGTGACAT AGTGGCCGTC ATAGAGACAT TGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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DUR1,2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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