LDH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	LDH1
Entrez Gene ID	852503
Full Name	triacylglycerol lipase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178552.2	NP_009763.2	triacylglycerol lipase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E23035g	XP_454991.1
Saccharomyces cerevisiae (baker's yeast)	LDH1	NP_009763.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ldh1p is not required for the function and biogenesis of peroxisomes, and targeting of Ldh1p to lipid droplets occurs independently of the PTS1 receptor Pex5p. [Ldh1p]
Title: The putative Saccharomyces cerevisiae hydrolase Ldh1p is localized to lipid droplets.

Ldh1p is thought to play a role in maintaining the lipid homeostasis in yeast by regulating both phospholipid and nonpolar lipid levels
Title: Involvement of the Saccharomyces cerevisiae hydrolase Ldh1p in lipid homeostasis.

The following LDH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LDH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01904	NM_001178552.2 Latest version!	Saccharomyces cerevisiae S288C triacylglycerol lipase (LDH1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LDH1 ( NM_001178552.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009763.2 Saccharomyces cerevisiae S288C triacylglycerol lipase (LDH1), partial mRNA.

Price & Availability↑

CloneID	OSi01904
Clone ID Related Accession (Same CDS sequence)	NM_001178552.2
Accession Version	NM_001178552.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	triacylglycerol lipase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178552.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATATGG CAGAACGTGC AGAAGCAACA AAAAGTTGGT CCTGTGAGCC CCTTTCTGGA 
AAGACGCTAG AAGAGATTGT TCAAAATGCA GAAAATGCAG CTGATTTGGT AGCTTACATT 
CGCAAACCAG AGGTAGATTT GGACTTTAGG TTGAAGTTTA TCGCAGAACA TGAGGAGTTT 
TTTAATGTAC AACTATCGGA TCGAAATTCA AGAATAAGGA CGTGTCACAA CTTGAGCGAT 
AAAGGGATAC GGGGTGATAC TGTTTTCGTC TTCGTGCCTG GGTTGGCTGG CAACTTAGAA 
CAATTTGAAC CCTTACTCGA GCTTGTGGAC TCTGATCAAA AGGCATTTTT GACGTTAGAT 
CTGCCCGGCT TTGGTCATAG TTCAGAATGG AGCGACTACC CCATGCTGAA AGTGGTGGAA 
CTTATTTTCG TTCTTGTATG TGACGTCTTA AGAAAATGGT CTACAGCGGT GCCGAATAAT 
GATAACGTAA ATCCATTTAA CGGTCATAAG ATAGTGCTTG TAGGGCATTC TATGGGTTGT 
TTTCTGGCAT GTCATCTTTA CGAGCAGCAC ATGGCTGATA CAAAAGCGGT ACAAACGCTA 
GTTTTGTTGA CGCCCCCCAA AGCACATATC GAGCAGCTTT CTAAGGATAA ACATATTATC 
CAGTGGGCTC TCTATGGTGT GTTTAAATTG CCATGGCTAT TTGACGTTTA TAGAAACAAA 
TTCGATCAGG TAAAAGGTCT ACAAAGTTCC GGCATCAAGC AGTATTTTTA TCAACAAGGT 
GATGATGTCA AGTTGAAATA CAGGAAGTTC TGGCAATTTA AAAACAACAT AAGCAACAAA 
AGTAGAACCA TCATAGGTTA TTTACTAGGA TGGGAAACTG TTGATTGGGT TAAATTCAAT 
GGTGTATTGA CGCAAACGGA TATGAAACAG AAAATTATAA TATTTGGCGC TGAAAAAGAT 
CCTATAGCTC CTATTGAAAA TTTGGAATTT TACAAACAAA CTATAAATAA AGAATGTCTT 
CGTAAAGTGA TTATTTTACC TGACTGTTCT CATAATTTAT GCTTCGACCG TCCAGAATTG 
GTGTGTGAAA ACTTTCAACG AGAGGTGATT GATAATTCCA AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009763.2
CDS	1..1128
Translation	MNMAERAEATKSWSCEPLSGKTLEEIVQNAENAADLVAYIRKPEVDLDFRLKFIAEHEEFFNVQLSDRNSRIRTCHNLSDKGIRGDTVFVFVPGLAGNLEQFEPLLELVDSDQKAFLTLDLPGFGHSSEWSDYPMLKVVELIFVLVCDVLRKWSTAVPNNDNVNPFNGHKIVLVGHSMGCFLACHLYEQHMADTKAVQTLVLLTPPKAHIEQLSKDKHIIQWALYGVFKLPWLFDVYRNKFDQVKGLQSSGIKQYFYQQGDDVKLKYRKFWQFKNNISNKSRTIIGYLLGWETVDWVKFNGVLTQTDMKQKIIIFGAEKDPIAPIENLEFYKQTINKECLRKVIILPDCSHNLCFDRPELVCENFQREVIDNSKL

Target ORF information:

RefSeq Version	NM_001178552.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C triacylglycerol lipase (LDH1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178552.2

ORF Insert Sequence:

ATGAATATGG CAGAACGTGC AGAAGCAACA AAAAGTTGGT CCTGTGAGCC CCTTTCTGGA 
AAGACGCTAG AAGAGATTGT TCAAAATGCA GAAAATGCAG CTGATTTGGT AGCTTACATT 
CGCAAACCAG AGGTAGATTT GGACTTTAGG TTGAAGTTTA TCGCAGAACA TGAGGAGTTT 
TTTAATGTAC AACTATCGGA TCGAAATTCA AGAATAAGGA CGTGTCACAA CTTGAGCGAT 
AAAGGGATAC GGGGTGATAC TGTTTTCGTC TTCGTGCCTG GGTTGGCTGG CAACTTAGAA 
CAATTTGAAC CCTTACTCGA GCTTGTGGAC TCTGATCAAA AGGCATTTTT GACGTTAGAT 
CTGCCCGGCT TTGGTCATAG TTCAGAATGG AGCGACTACC CCATGCTGAA AGTGGTGGAA 
CTTATTTTCG TTCTTGTATG TGACGTCTTA AGAAAATGGT CTACAGCGGT GCCGAATAAT 
GATAACGTAA ATCCATTTAA CGGTCATAAG ATAGTGCTTG TAGGGCATTC TATGGGTTGT 
TTTCTGGCAT GTCATCTTTA CGAGCAGCAC ATGGCTGATA CAAAAGCGGT ACAAACGCTA 
GTTTTGTTGA CGCCCCCCAA AGCACATATC GAGCAGCTTT CTAAGGATAA ACATATTATC 
CAGTGGGCTC TCTATGGTGT GTTTAAATTG CCATGGCTAT TTGACGTTTA TAGAAACAAA 
TTCGATCAGG TAAAAGGTCT ACAAAGTTCC GGCATCAAGC AGTATTTTTA TCAACAAGGT 
GATGATGTCA AGTTGAAATA CAGGAAGTTC TGGCAATTTA AAAACAACAT AAGCAACAAA 
AGTAGAACCA TCATAGGTTA TTTACTAGGA TGGGAAACTG TTGATTGGGT TAAATTCAAT 
GGTGTATTGA CGCAAACGGA TATGAAACAG AAAATTATAA TATTTGGCGC TGAAAAAGAT 
CCTATAGCTC CTATTGAAAA TTTGGAATTT TACAAACAAA CTATAAATAA AGAATGTCTT 
CGTAAAGTGA TTATTTTACC TGACTGTTCT CATAATTTAT GCTTCGACCG TCCAGAATTG 
GTGTGTGAAA ACTTTCAACG AGAGGTGATT GATAATTCCA AATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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LDH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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