DER1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DER1
Entrez Gene ID	852500
Full Name	derlin
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178549.1	NP_009760.1	derlin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that N-terminal acetylation of Der1 regulates its degradation by the Hrd1 ligase.
Title: N-terminal acetylation of the yeast Derlin Der1 is essential for Hrd1 ubiquitin-ligase activity toward luminal ER substrates.

Data show that Der1p appears to have a role in a pathway that is parallel to that involving Hrd3p.
Title: Recognition of an ERAD-L substrate analyzed by site-specific in vivo photocrosslinking.

Der1p degradation complex is enriched in a Cdc48p cofactor Ufd1p.
Title: The ER-associated degradation component Der1p and its homolog Dfm1p are contained in complexes with distinct cofactors of the ATPase Cdc48p.

The following DER1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DER1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01901	NM_001178549.1 Latest version!	Saccharomyces cerevisiae S288C derlin (DER1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DER1 ( NM_001178549.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009760.1 Saccharomyces cerevisiae S288C derlin (DER1), partial mRNA.

Price & Availability↑

CloneID	OSi01901
Clone ID Related Accession (Same CDS sequence)	NM_001178549.1
Accession Version	NM_001178549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	derlin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATGCTG TAATACTGAA TCTCTTAGGC GACATTCCTT TGGTCACAAG ATTATGGACA 
ATTGGCTGTC TTGTACTATC AGGTCTCACA AGTCTCCGGA TTGTGGATCC AGGGAAGGTA 
GTGTACAGTT ATGATTTAGT ATTCAAAAAG GGACAATATG GAAGACTACT TTATTCGATA 
TTCGATTACG GCGCATTTAA TTGGATATCC ATGATAAACA TCTTTGTCAG CGCTAATCAC 
TTATCAACTT TGGAAAACTC ATTCAATCTG AGAAGAAAAT TCTGTTGGAT AATATTTTTA 
CTGTTGGTGA TACTGGTAAA GATGACCAGC ATTGAACAAC CTGCAGCATC ACTCGGTGTG 
TTATTGCATG AGAATCTCGT GTACTACGAA CTGAAAAAGA ACGGAAACCA AATGAACGTA 
CGATTCTTCG GTGCCATTGA TGTTTCACCA TCTATATTCC CAATCTACAT GAATGCAGTA 
ATGTATTTTG TATATAAGCG TAGCTGGTTA GAAATTGCCA TGAATTTCAT GCCAGGTCAC 
GTAATTTACT ACATGGATGA TATAATAGGG AAGATTTATG GCATCGATTT GTGTAAATCT 
CCGTACGACT GGTTCCGCAA CACTGAAACA CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009760.1
CDS	1..636
Translation	MDAVILNLLGDIPLVTRLWTIGCLVLSGLTSLRIVDPGKVVYSYDLVFKKGQYGRLLYSIFDYGAFNWISMINIFVSANHLSTLENSFNLRRKFCWIIFLLLVILVKMTSIEQPAASLGVLLHENLVYYELKKNGNQMNVRFFGAIDVSPSIFPIYMNAVMYFVYKRSWLEIAMNFMPGHVIYYMDDIIGKIYGIDLCKSPYDWFRNTETP

Target ORF information:

RefSeq Version	NM_001178549.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C derlin (DER1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178549.1

ORF Insert Sequence:

ATGGATGCTG TAATACTGAA TCTCTTAGGC GACATTCCTT TGGTCACAAG ATTATGGACA 
ATTGGCTGTC TTGTACTATC AGGTCTCACA AGTCTCCGGA TTGTGGATCC AGGGAAGGTA 
GTGTACAGTT ATGATTTAGT ATTCAAAAAG GGACAATATG GAAGACTACT TTATTCGATA 
TTCGATTACG GCGCATTTAA TTGGATATCC ATGATAAACA TCTTTGTCAG CGCTAATCAC 
TTATCAACTT TGGAAAACTC ATTCAATCTG AGAAGAAAAT TCTGTTGGAT AATATTTTTA 
CTGTTGGTGA TACTGGTAAA GATGACCAGC ATTGAACAAC CTGCAGCATC ACTCGGTGTG 
TTATTGCATG AGAATCTCGT GTACTACGAA CTGAAAAAGA ACGGAAACCA AATGAACGTA 
CGATTCTTCG GTGCCATTGA TGTTTCACCA TCTATATTCC CAATCTACAT GAATGCAGTA 
ATGTATTTTG TATATAAGCG TAGCTGGTTA GAAATTGCCA TGAATTTCAT GCCAGGTCAC 
GTAATTTACT ACATGGATGA TATAATAGGG AAGATTTATG GCATCGATTT GTGTAAATCT 
CCGTACGACT GGTTCCGCAA CACTGAAACA CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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DER1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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