FZO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FZO1
Entrez Gene ID	852477
Full Name	mitofusin
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178527.1	NP_009738.1	mitofusin

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	fzo1	NP_595241.1
Eremothecium gossypii	AGOS_ABR195C	NP_983144.2
Saccharomyces cerevisiae (baker's yeast)	FZO1	NP_009738.1
Kluyveromyces lactis	KLLA0E24179g	XP_455041.1
Neurospora crassa	NCU00436	XP_956700.1
Magnaporthe oryzae (rice blast fungus)	MGG_05209	XP_003712754.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest a sequence of events in early mitochondrial fusion where Fzo1 GTPase-domain-dependent tethering leads to recruitment of SCF(Mdm30) and ubiquitin-mediated degradation of Fzo1, which facilitates mitochondrial fusion.
Title: Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion.

Fzo1 assembles into homo-dimers, depending on Ugo1 and on GTP binding to Fzo1.
Title: Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion.

Results identify diverse effects of CMT2A mutations, including a possible role for Mfn2 ubiquitylation and degradation in CMT2A pathogenesis, and provide evidence for a link between Fzo1 GTP hydrolysis, ubiquitylation, and mitochondrial fusion.
Title: A mutation associated with CMT2A neuropathy causes defects in Fzo1 GTP hydrolysis, ubiquitylation, and protein turnover.

This study provides a framework for developing an understanding of the function of Mdm30p-mediated Fzo1p degradation in the multistep process of mitochondrial fusion.
Title: Ubiquitin-proteasome-dependent degradation of a mitofusin, a critical regulator of mitochondrial fusion.

Fzo1 oligomer domain interactions are essential for mitochondrial fusion
Title: Domain interactions within Fzo1 oligomers are essential for mitochondrial fusion.

The following FZO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FZO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01880	NM_001178527.1 Latest version!	Saccharomyces cerevisiae S288C mitofusin (FZO1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FZO1 ( NM_001178527.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009738.1 Saccharomyces cerevisiae S288C mitofusin (FZO1), partial mRNA.

Price & Availability↑

CloneID	OSi01880
Clone ID Related Accession (Same CDS sequence)	NM_001178527.1
Accession Version	NM_001178527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mitofusin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGAAG GAAAACAACA ATTCAAAGAC AGCAATAAAC CGCACAAGGA CTCCACAGAT 
CAGGACGATG ATGCCGCCAC AATAGTACCA CAAACTCTTA CTTACTCACG CAACGAAGGT 
CATTTTTTAG GCAGTAATTT CCATGGAGTA ACTGATGACC GTACCACTTT GTTTGATGGT 
GAAGAGGGGC GTAGGGAGGA TGATTTGTTG CCCTCATTGC GTTCCTCCAA CTCAAAAGCA 
CACTTAATTT CTTCACAACT TAGCCAGTGG AATTATAACA ACAATAGAGT ACTTTTAAAA 
AGATCTATAT TAAAGACACA AGCTTTTATG GATCAACTTC AAGAAGAAAA TAATATTCGC 
CCGATTTTTA TTGCTGCAAA TGATGAACGG GAAAAATTAC ATGTTCTACA ACTGAATATC 
AAGCTAGATG GTCAGTACAA TACCAAAGAA AAGAACGGTT TCAACATTGA GAAAAAAGCT 
TTATCAAAAT TATTTCACTC TCAGATCGTA TCAGTGACAA ATCATTTAAA CGCTTTGAAA 
AAAAGAGTGG ATGACGTTTC ATCTAAAGTA TTCATCACAG GTGATGTAAA TACTGGTAAA 
TCAGCTCTTT GCAACTCTCT ATTAAAGCAG CGTTTGCTAC CTGAGGATCA GCTACCATGT 
ACCAATGTAT TTTCCGAAAT ACTAGAGGCT CGGGAAAATG ACGGCATAGA GGAGGTTCAT 
GCCATACCAC TAAACATTGC TCCGACTCTC AAGGAAGCCA TTGATATGTA TTCAATACAA 
AATCCCAAGA CTTATGAAAT ACACACTTTG AAAGAACTTC CCGATTTGGT TCCCCAAAAC 
GGAAAATATG CTTTACTGAA GATTTACATA AAGGACGATA AAAGGCCTGC CTCTACTAGT 
CTATTAAGGA ATGGTACTGT TGATATTTCA TTAATTGACT CCCCTGGGTT GAACATGGAC 
TCGTTACAAA CCGCCGAAGT AATGTCCCGA CAAGAAGAAA TCGACTTAGT TATATTTGTT 
GTCAATGCAG AGAACCAGCT AACACTATCT GCCAAGGAGT TTATTTCTTT AGCATCTCGT 
GAGAAAAAAT TAATGTTTTT TGTTGTGAAA AAATTTGACA AAATCAGGGA CAAACAACGT 
TGTAAGGAGT TGATCCTAAA GCAAATCCGT GACCTGTCCC CAGAAACATA TAAACGTGCA 
GCTGATTTTG TCCACTTTGT TTCCAAAAAC GGAGATGAAT TGCCACATTA TCATAATGAA 
AATGACAATG AAGATCATGG TGACCGAAAA CCTGACGACG ACCCATATTC TAGCAGCGAT 
CCCGATCCGG ATTTTGACAG TCTGGAAGAT TCTCTACGCA ATTTTGTTTT GAAGAAAAGA 
TCACTTTCTA AATTGTTACC TGCCAAGACT TATCTATCGA AATTATTAAG CGATATAATA 
ATGATCTCCA AATCTAATAT GAAAATGTAT AGTGAAGAAG AAATAAAAAT TAACGAACAA 
CTGGAAACAT TGAGACCTGA AATTTTGAGC GCTAGAGCAA AGTGCAATGA TTTAACAACT 
TCCGTTGATC AAATGGCCGA GCAAACAATT ACAATGACTT ATAACAATAC TAAAGAAGCA 
CTTCTCAATG CGTTGGATGT TCCGCTACAC GAATATCCGA AATATCAGGG CCTCGGTCAA 
ATTTATGACT TCATATTTTC AACAGAAGCG TTCATAGCAA ATCAGATTGA TGAGTCAATA 
GGATCAAGTG AATTATTTGC CAAGCAAAAG ACAGATTTAC TGGTAAAAAA AATTTATGAG 
ATTGGAAAAA ATGAACTTGG TGATGATTTC ATGTGTGAAC GTGTTTTCAG AAGCGAACTA 
ATGTTTAGAA AAAGGAAGCA TTTGATAGGA AAAAGGCTTA AAGTATCTTT GTCAATAACT 
GATTTGTTTG CTCCAACTTG GAAAGGCTTT TTGTCTTACT TAAGCTGGCA GAAACCTGTC 
ACAGCACCAC TGCCGGATAT AGAAGGACAA ACTAACGAGG GTCAGATTGG CCTAATGAAG 
TATTTGGGTT TGAAAAATTA TCCTTTGACA CAATATTGGT CAAGACCATC ATTGCTCTTC 
ACATCAAAAA TTCCCACTCT AACGCTGTAC TTTCTAGGAA GTACAAAGGT TGTGGGGAAC 
ATTATATTAA ACGGCATTAA ATTATCGTCG TGGAGCTCAC TCAAGAAGTT ATCAGTTCCG 
GTTATAGTGG TAGGTTCCTT GTTGGGACTT ACGTATTTAA TTCATGACCT CCCCCGTGCC 
TTACCAATGA ATCTTTCCAT TAAATACAAA AGAAAGTTGC AAGAACTGGA TTACATTCAT 
CTTAACGCTC AAAGAACCTC AAATGAAGTA CGTGATGTTT TGCGTGTACC TACACGTGAA 
ATTCTAAGGT CGTGTGAAAT AATTATGGAT AAAAAACAAA TCACTAAGAA AGAATTGGAA 
AACAAAAAAG AAAGTAACTT GTTGTCAATC AAATTCTTCC AATCTCTATA CGAAGGAACC 
GTGGCTCAAA AATTGATGGT GGAAGAAATA AATTTAGACA TCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009738.1
CDS	1..2568
Translation	MSEGKQQFKDSNKPHKDSTDQDDDAATIVPQTLTYSRNEGHFLGSNFHGVTDDRTTLFDGEEGRREDDLLPSLRSSNSKAHLISSQLSQWNYNNNRVLLKRSILKTQAFMDQLQEENNIRPIFIAANDEREKLHVLQLNIKLDGQYNTKEKNGFNIEKKALSKLFHSQIVSVTNHLNALKKRVDDVSSKVFITGDVNTGKSALCNSLLKQRLLPEDQLPCTNVFSEILEARENDGIEEVHAIPLNIAPTLKEAIDMYSIQNPKTYEIHTLKELPDLVPQNGKYALLKIYIKDDKRPASTSLLRNGTVDISLIDSPGLNMDSLQTAEVMSRQEEIDLVIFVVNAENQLTLSAKEFISLASREKKLMFFVVKKFDKIRDKQRCKELILKQIRDLSPETYKRAADFVHFVSKNGDELPHYHNENDNEDHGDRKPDDDPYSSSDPDPDFDSLEDSLRNFVLKKRSLSKLLPAKTYLSKLLSDIIMISKSNMKMYSEEEIKINEQLETLRPEILSARAKCNDLTTSVDQMAEQTITMTYNNTKEALLNALDVPLHEYPKYQGLGQIYDFIFSTEAFIANQIDESIGSSELFAKQKTDLLVKKIYEIGKNELGDDFMCERVFRSELMFRKRKHLIGKRLKVSLSITDLFAPTWKGFLSYLSWQKPVTAPLPDIEGQTNEGQIGLMKYLGLKNYPLTQYWSRPSLLFTSKIPTLTLYFLGSTKVVGNIILNGIKLSSWSSLKKLSVPVIVVGSLLGLTYLIHDLPRALPMNLSIKYKRKLQELDYIHLNAQRTSNEVRDVLRVPTREILRSCEIIMDKKQITKKELENKKESNLLSIKFFQSLYEGTVAQKLMVEEINLDID

Target ORF information:

RefSeq Version	NM_001178527.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mitofusin (FZO1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178527.1

ORF Insert Sequence:

ATGTCTGAAG GAAAACAACA ATTCAAAGAC AGCAATAAAC CGCACAAGGA CTCCACAGAT 
CAGGACGATG ATGCCGCCAC AATAGTACCA CAAACTCTTA CTTACTCACG CAACGAAGGT 
CATTTTTTAG GCAGTAATTT CCATGGAGTA ACTGATGACC GTACCACTTT GTTTGATGGT 
GAAGAGGGGC GTAGGGAGGA TGATTTGTTG CCCTCATTGC GTTCCTCCAA CTCAAAAGCA 
CACTTAATTT CTTCACAACT TAGCCAGTGG AATTATAACA ACAATAGAGT ACTTTTAAAA 
AGATCTATAT TAAAGACACA AGCTTTTATG GATCAACTTC AAGAAGAAAA TAATATTCGC 
CCGATTTTTA TTGCTGCAAA TGATGAACGG GAAAAATTAC ATGTTCTACA ACTGAATATC 
AAGCTAGATG GTCAGTACAA TACCAAAGAA AAGAACGGTT TCAACATTGA GAAAAAAGCT 
TTATCAAAAT TATTTCACTC TCAGATCGTA TCAGTGACAA ATCATTTAAA CGCTTTGAAA 
AAAAGAGTGG ATGACGTTTC ATCTAAAGTA TTCATCACAG GTGATGTAAA TACTGGTAAA 
TCAGCTCTTT GCAACTCTCT ATTAAAGCAG CGTTTGCTAC CTGAGGATCA GCTACCATGT 
ACCAATGTAT TTTCCGAAAT ACTAGAGGCT CGGGAAAATG ACGGCATAGA GGAGGTTCAT 
GCCATACCAC TAAACATTGC TCCGACTCTC AAGGAAGCCA TTGATATGTA TTCAATACAA 
AATCCCAAGA CTTATGAAAT ACACACTTTG AAAGAACTTC CCGATTTGGT TCCCCAAAAC 
GGAAAATATG CTTTACTGAA GATTTACATA AAGGACGATA AAAGGCCTGC CTCTACTAGT 
CTATTAAGGA ATGGTACTGT TGATATTTCA TTAATTGACT CCCCTGGGTT GAACATGGAC 
TCGTTACAAA CCGCCGAAGT AATGTCCCGA CAAGAAGAAA TCGACTTAGT TATATTTGTT 
GTCAATGCAG AGAACCAGCT AACACTATCT GCCAAGGAGT TTATTTCTTT AGCATCTCGT 
GAGAAAAAAT TAATGTTTTT TGTTGTGAAA AAATTTGACA AAATCAGGGA CAAACAACGT 
TGTAAGGAGT TGATCCTAAA GCAAATCCGT GACCTGTCCC CAGAAACATA TAAACGTGCA 
GCTGATTTTG TCCACTTTGT TTCCAAAAAC GGAGATGAAT TGCCACATTA TCATAATGAA 
AATGACAATG AAGATCATGG TGACCGAAAA CCTGACGACG ACCCATATTC TAGCAGCGAT 
CCCGATCCGG ATTTTGACAG TCTGGAAGAT TCTCTACGCA ATTTTGTTTT GAAGAAAAGA 
TCACTTTCTA AATTGTTACC TGCCAAGACT TATCTATCGA AATTATTAAG CGATATAATA 
ATGATCTCCA AATCTAATAT GAAAATGTAT AGTGAAGAAG AAATAAAAAT TAACGAACAA 
CTGGAAACAT TGAGACCTGA AATTTTGAGC GCTAGAGCAA AGTGCAATGA TTTAACAACT 
TCCGTTGATC AAATGGCCGA GCAAACAATT ACAATGACTT ATAACAATAC TAAAGAAGCA 
CTTCTCAATG CGTTGGATGT TCCGCTACAC GAATATCCGA AATATCAGGG CCTCGGTCAA 
ATTTATGACT TCATATTTTC AACAGAAGCG TTCATAGCAA ATCAGATTGA TGAGTCAATA 
GGATCAAGTG AATTATTTGC CAAGCAAAAG ACAGATTTAC TGGTAAAAAA AATTTATGAG 
ATTGGAAAAA ATGAACTTGG TGATGATTTC ATGTGTGAAC GTGTTTTCAG AAGCGAACTA 
ATGTTTAGAA AAAGGAAGCA TTTGATAGGA AAAAGGCTTA AAGTATCTTT GTCAATAACT 
GATTTGTTTG CTCCAACTTG GAAAGGCTTT TTGTCTTACT TAAGCTGGCA GAAACCTGTC 
ACAGCACCAC TGCCGGATAT AGAAGGACAA ACTAACGAGG GTCAGATTGG CCTAATGAAG 
TATTTGGGTT TGAAAAATTA TCCTTTGACA CAATATTGGT CAAGACCATC ATTGCTCTTC 
ACATCAAAAA TTCCCACTCT AACGCTGTAC TTTCTAGGAA GTACAAAGGT TGTGGGGAAC 
ATTATATTAA ACGGCATTAA ATTATCGTCG TGGAGCTCAC TCAAGAAGTT ATCAGTTCCG 
GTTATAGTGG TAGGTTCCTT GTTGGGACTT ACGTATTTAA TTCATGACCT CCCCCGTGCC 
TTACCAATGA ATCTTTCCAT TAAATACAAA AGAAAGTTGC AAGAACTGGA TTACATTCAT 
CTTAACGCTC AAAGAACCTC AAATGAAGTA CGTGATGTTT TGCGTGTACC TACACGTGAA 
ATTCTAAGGT CGTGTGAAAT AATTATGGAT AAAAAACAAA TCACTAAGAA AGAATTGGAA 
AACAAAAAAG AAAGTAACTT GTTGTCAATC AAATTCTTCC AATCTCTATA CGAAGGAACC 
GTGGCTCAAA AATTGATGGT GGAAGAAATA AATTTAGACA TCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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FZO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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