SLI15 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SLI15
Entrez Gene ID	852453
Full Name	Sli15p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178504.3	NP_009714.3	Sli15p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SLI15	NP_009714.3
Eremothecium gossypii	AGOS_AGL269W	NP_986398.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ipl1-dependent phosphorylation of Sli15 therefore inhibits its association with microtubules both in vivo and in vitro and may negatively regulate the tension checkpoint mechanism.
Title: Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiae.

phosphorylation of INCENP/Sli15 is required for activation of the kinase Aurora B/Ipl1 and can occur in trans.
Title: Phosphorylation regulates kinase and microtubule binding activities of the budding yeast chromosomal passenger complex in vitro.

an engineered truncation of the Sli15 subunit of budding yeast chromosomal passenger complex that eliminates association with the inner centromere nevertheless supports proper chromosome segregation during both mitosis and meiosis
Title: Tension sensing by Aurora B kinase is independent of survivin-based centromere localization.

Cdk1 and Ipl1/Aurora cooperatively modulate microtubule dynamics and that Ipl1/Aurora-dependent phosphorylation of Sli15 controls spindle function by excluding the CPC from spindle regions engaged in microtubule polymerization
Title: Ipl1/Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC-spindle interaction to ensure proper microtubule dynamics.

dephosphorylation by Cdc14 inactivates the mitotic checkpoint
Title: Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onset.

The following SLI15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLI15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01858	NM_001178504.3 Latest version!	Saccharomyces cerevisiae S288C Sli15p (SLI15), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLI15 ( NM_001178504.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009714.3 Saccharomyces cerevisiae S288C Sli15p (SLI15), partial mRNA.

Price & Availability↑

CloneID	OSi01858
Clone ID Related Accession (Same CDS sequence)	NM_001178504.3
Accession Version	NM_001178504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Sli15p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178504.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGACTGGG CAATCAAAGC AGCTAGGAAG AAAACTCAAA GGAAGCCAGG CTCTACCCGT 
TCAATCATAG AAACCCTCGA TGATCTAAAT AATCTAACAA CAGATGCACA TTCAGAAATA 
AATCAACGAT TGTACGAAAG TAGTGAATGG TTAAGAAATA ATGTTTATAT GAATACACTC 
AAGTATGAAG ACAAAAAGAT GGAAGAGTCT TTAATTAGCC CCGAAAATAC GCATAACAAA 
ATGGATGTCG AATTTCCTAA AATGAAAGGA GAATATGAAC TTTCTAACTC CCAGAATGAT 
GCCGCAAAAG ACGTTACTAA GACACCCAGA AATGGATTAC ATAACGATAA AAGTATCACG 
CCTAAATCAC TCCGCAGGAA GGAAGTCACC GAAGGAATGA ATAGATTTAG TATACATGAT 
ACCAATAAAA GCCCGGTTGA GCCATTGAAT AGCGTTAAAG TCGACGCCAA TGAAAGCGAA 
AAATCCTCAC CATGGTCACC TTACAAAGTT GAAAAGGTTC TAAGAGAATC CTCCAAGACT 
TCGGAGTCTC CGATTAATAC AAAACGCTTC GATAATCAAA CATGGGCGGC TAAGGAAGAA 
ATGGAGAATG AACCTATACT TCAAGCTTTA AAGAAAGCTG AATCAGTAAA GGTGAAACCA 
CCACCGAATT CTGGGATAGC GAGATCTCAA AGAAGGTCGA ATATGTTTGT TCCACTGCCG 
AATAAAGATC CTCTTATTAT TCAACACATT CCACCAACAA AATCTTCAGG ATCAATACCA 
AAAGTACGAA CAGTAAAGGA ATCACCAATC GCATTTAAAA AAAAATCTAC GATAAATAGT 
CCTGCTATAA GAGCTGTGGA AAACAGTGAT ACCGCGGGAT CTACAAAAGC ATCCAGTGTT 
TTCGATAGGC TATCATCTAT TCCAACAAAA TCATTTGAAA ACAAAATTTC CCGTGGAAAC 
GTTGGTCACA AATACTCCTC TTCATCAATC GATTTAACAG GCTCCCCCAT GAAAAAAGTT 
TCTCAAAAGT TTAAGTCAAT AAACTCCACG GATACTGATA TGCAAGAAGC GTTAAGAGAT 
ATATTCTCAG TAAAAAATAA AATAACCAAA AATAATTCAC CCAAAGGAAA AAACTCTCGA 
AAATCCTCTA TTCCGAGGTT TGATAAGACT TCTTTGAAGC TAACGACACA CAAAAAGCTA 
GCAATCATTG CAGAACAGAA AAAGAAGTCC AAACACTCTA GTGATGTTCA TAAAACTGGT 
TCAAGACCGC ACAGTATTTC TCCAACAAAA ATAAGTGTCG ATTCAAGCTC GCCATCTAAA 
GAAGTTAAAA ATTATTACCA AAGCCCTGTA AGAGGTTATT TGAGACCAAC AAAAGCGTCT 
ATTTCGCCTA ATAAAAATAA AAATTTAACA ACGTCGCAAA CGCCACATCG TTTGAAAATT 
AAAGAGAAAA CATTAAGAAA ACTTTCTCCG AATATTGCTG ACATCTCCAA GCCAGAGTCT 
CGTAAATCCA AAAATTATCG GCTTACAAAC CTTCAATTAC TTCCACCAGC AGAGGCAGAA 
CGAGATGACT TAAAAAAAAA GTTTGATAAA AGGTTATCAG GAATTATGAG GTCTCAACAG 
GAACATCATC GGCGTAAACA AGAGAAACAA AAAAGGATGT CGCATTTGGA ACAAGATCTA 
AAAAAGCAAA CAAGCTTTAG TAATGACTAC AAGGACATAC GTCTAAAAGA ATCTTTGGCT 
CCTTTCGATA ATCATGTGCG AGATACCATC AACAAAAATA CTGCATTTAG TACCGACAAT 
ATATTGGCCA CAATAAATAC AGTTGACCAT CGAGAAATAA TCGGAAATGT GACCCCAAAG 
ATAGCCTCTG TCAATGATTC TTTACCAGAG ATAAATACTG ATTCTGAAGA TGAGGCTAGC 
GTAACTTTAG CGGCATGGGC TAAATCCCCG TATTTGCAAG AGCAATTGAT AAGACAGCAA 
GATATAAATC CACAAACTAT CTTTGGACCA ATTCCGCCTT TGCACACCGA TGAAATTTTC 
CCAAATCCTA GGCTAAACAG GTTGAAACCG CGTCAAATTG TGCCCAAAAG GTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009714.3
CDS	1..2097
Translation	MDWAIKAARKKTQRKPGSTRSIIETLDDLNNLTTDAHSEINQRLYESSEWLRNNVYMNTLKYEDKKMEESLISPENTHNKMDVEFPKMKGEYELSNSQNDAAKDVTKTPRNGLHNDKSITPKSLRRKEVTEGMNRFSIHDTNKSPVEPLNSVKVDANESEKSSPWSPYKVEKVLRESSKTSESPINTKRFDNQTWAAKEEMENEPILQALKKAESVKVKPPPNSGIARSQRRSNMFVPLPNKDPLIIQHIPPTKSSGSIPKVRTVKESPIAFKKKSTINSPAIRAVENSDTAGSTKASSVFDRLSSIPTKSFENKISRGNVGHKYSSSSIDLTGSPMKKVSQKFKSINSTDTDMQEALRDIFSVKNKITKNNSPKGKNSRKSSIPRFDKTSLKLTTHKKLAIIAEQKKKSKHSSDVHKTGSRPHSISPTKISVDSSSPSKEVKNYYQSPVRGYLRPTKASISPNKNKNLTTSQTPHRLKIKEKTLRKLSPNIADISKPESRKSKNYRLTNLQLLPPAEAERDDLKKKFDKRLSGIMRSQQEHHRRKQEKQKRMSHLEQDLKKQTSFSNDYKDIRLKESLAPFDNHVRDTINKNTAFSTDNILATINTVDHREIIGNVTPKIASVNDSLPEINTDSEDEASVTLAAWAKSPYLQEQLIRQQDINPQTIFGPIPPLHTDEIFPNPRLNRLKPRQIVPKRS

Target ORF information:

RefSeq Version	NM_001178504.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Sli15p (SLI15), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178504.3

ORF Insert Sequence:

ATGGACTGGG CAATCAAAGC AGCTAGGAAG AAAACTCAAA GGAAGCCAGG CTCTACCCGT 
TCAATCATAG AAACCCTCGA TGATCTAAAT AATCTAACAA CAGATGCACA TTCAGAAATA 
AATCAACGAT TGTACGAAAG TAGTGAATGG TTAAGAAATA ATGTTTATAT GAATACACTC 
AAGTATGAAG ACAAAAAGAT GGAAGAGTCT TTAATTAGCC CCGAAAATAC GCATAACAAA 
ATGGATGTCG AATTTCCTAA AATGAAAGGA GAATATGAAC TTTCTAACTC CCAGAATGAT 
GCCGCAAAAG ACGTTACTAA GACACCCAGA AATGGATTAC ATAACGATAA AAGTATCACG 
CCTAAATCAC TCCGCAGGAA GGAAGTCACC GAAGGAATGA ATAGATTTAG TATACATGAT 
ACCAATAAAA GCCCGGTTGA GCCATTGAAT AGCGTTAAAG TCGACGCCAA TGAAAGCGAA 
AAATCCTCAC CATGGTCACC TTACAAAGTT GAAAAGGTTC TAAGAGAATC CTCCAAGACT 
TCGGAGTCTC CGATTAATAC AAAACGCTTC GATAATCAAA CATGGGCGGC TAAGGAAGAA 
ATGGAGAATG AACCTATACT TCAAGCTTTA AAGAAAGCTG AATCAGTAAA GGTGAAACCA 
CCACCGAATT CTGGGATAGC GAGATCTCAA AGAAGGTCGA ATATGTTTGT TCCACTGCCG 
AATAAAGATC CTCTTATTAT TCAACACATT CCACCAACAA AATCTTCAGG ATCAATACCA 
AAAGTACGAA CAGTAAAGGA ATCACCAATC GCATTTAAAA AAAAATCTAC GATAAATAGT 
CCTGCTATAA GAGCTGTGGA AAACAGTGAT ACCGCGGGAT CTACAAAAGC ATCCAGTGTT 
TTCGATAGGC TATCATCTAT TCCAACAAAA TCATTTGAAA ACAAAATTTC CCGTGGAAAC 
GTTGGTCACA AATACTCCTC TTCATCAATC GATTTAACAG GCTCCCCCAT GAAAAAAGTT 
TCTCAAAAGT TTAAGTCAAT AAACTCCACG GATACTGATA TGCAAGAAGC GTTAAGAGAT 
ATATTCTCAG TAAAAAATAA AATAACCAAA AATAATTCAC CCAAAGGAAA AAACTCTCGA 
AAATCCTCTA TTCCGAGGTT TGATAAGACT TCTTTGAAGC TAACGACACA CAAAAAGCTA 
GCAATCATTG CAGAACAGAA AAAGAAGTCC AAACACTCTA GTGATGTTCA TAAAACTGGT 
TCAAGACCGC ACAGTATTTC TCCAACAAAA ATAAGTGTCG ATTCAAGCTC GCCATCTAAA 
GAAGTTAAAA ATTATTACCA AAGCCCTGTA AGAGGTTATT TGAGACCAAC AAAAGCGTCT 
ATTTCGCCTA ATAAAAATAA AAATTTAACA ACGTCGCAAA CGCCACATCG TTTGAAAATT 
AAAGAGAAAA CATTAAGAAA ACTTTCTCCG AATATTGCTG ACATCTCCAA GCCAGAGTCT 
CGTAAATCCA AAAATTATCG GCTTACAAAC CTTCAATTAC TTCCACCAGC AGAGGCAGAA 
CGAGATGACT TAAAAAAAAA GTTTGATAAA AGGTTATCAG GAATTATGAG GTCTCAACAG 
GAACATCATC GGCGTAAACA AGAGAAACAA AAAAGGATGT CGCATTTGGA ACAAGATCTA 
AAAAAGCAAA CAAGCTTTAG TAATGACTAC AAGGACATAC GTCTAAAAGA ATCTTTGGCT 
CCTTTCGATA ATCATGTGCG AGATACCATC AACAAAAATA CTGCATTTAG TACCGACAAT 
ATATTGGCCA CAATAAATAC AGTTGACCAT CGAGAAATAA TCGGAAATGT GACCCCAAAG 
ATAGCCTCTG TCAATGATTC TTTACCAGAG ATAAATACTG ATTCTGAAGA TGAGGCTAGC 
GTAACTTTAG CGGCATGGGC TAAATCCCCG TATTTGCAAG AGCAATTGAT AAGACAGCAA 
GATATAAATC CACAAACTAT CTTTGGACCA ATTCCGCCTT TGCACACCGA TGAAATTTTC 
CCAAATCCTA GGCTAAACAG GTTGAAACCG CGTCAAATTG TGCCCAAAAG GTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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SLI15 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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