IRA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	IRA1
Entrez Gene ID	852437
Full Name	GTPase-activating protein IRA1
Synonyms	GLC1,PPD1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178488.3	NP_009698.3	GTPase-activating protein IRA1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	IRA1	NP_009698.3
Eremothecium gossypii	AGOS_AER025C	NP_984885.2
Saccharomyces cerevisiae (baker's yeast)	IRA2	NP_014560.1
Kluyveromyces lactis	KLLA0E17887g	XP_454757.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Roles of two protein phosphatases, Reg1-Glc7 and Sit4, and glycogen synthesis in regulation of SNF1 protein kinase.
Title: Roles of two protein phosphatases, Reg1-Glc7 and Sit4, and glycogen synthesis in regulation of SNF1 protein kinase.

Ira1 and Ira2 affect glycerophosphoinositol production and transport.
Title: Neurofibromin homologs Ira1 and Ira2 affect glycerophosphoinositol production and transport in Saccharomyces cerevisiae.

Gpb1/2 bind to a conserved C-terminal domain of Ira1/2, and loss of Gpb1/2 results in a destabilization of Ira1 and Ira2, leading to elevated levels of Ras2-GTP and unbridled cAMP-PKA signaling.
Title: The kelch proteins Gpb1 and Gpb2 inhibit Ras activity via association with the yeast RasGAP neurofibromin homologs Ira1 and Ira2.

The following IRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01843	NM_001178488.3 Latest version!	Saccharomyces cerevisiae S288C GTPase-activating protein IRA1 (IRA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRA1 ( NM_001178488.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009698.3 Saccharomyces cerevisiae S288C GTPase-activating protein IRA1 (IRA1), partial mRNA.

Price & Availability↑

CloneID	OSi01843
Clone ID Related Accession (Same CDS sequence)	NM_001178488.3
Accession Version	NM_001178488.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	GTPase-activating protein IRA1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178488.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATCAAA GCGATCCGCA AGACAAAAAA AATTTCCCAA TGGAATACTC TTTGACCAAG 
CATCTTTTTT TTGATAGGCT TCTACTTGTT CTTCCCATAG AATCTAATTT GAAAACATAT 
GCTGATGTGG AGGCAGATTC AGTTTTCAAT TCGTGTCGGT CCATCATTTT GAATATAGCC 
ATCACTAAGG ACTTGAACCC GATTATCGAA AACACATTAG GTTTAATTGA CTTGATTGTG 
CAAGATGAAG AAATTACGTC TGACAATATT ACAGATGATA TTGCCCATTC TATATTGGTT 
CTTTTGAGAT TACTGAGTGA TGTTTTTGAG TATTACTGGG ATCAAAACAA TGACTTCAAG 
AAAATTAGAA ACGATAATTA CAAACCGGGA TTTTCAAGTC ACAGGCCAAA CTTCCATACA 
TCTAGGCCAA AGCACACGAG AATCAATCCA GCTTTGGCGA CGATGTTACT ATGTAAAATT 
TCTAAGCTGA AGTTCAATAC AAGAACTTTA AAGGTTTTAC AGAACATGTC TCACCATCTT 
TCTGGCAGCG CTACTATCTC AAAATCGAGT ATTTTACCCG ATTCACAGGA ATTTTTACAA 
AAGAGAAACT ATCCAGCATA TACCGAGAAA ATAGATTTAA CAATAGATTA TATCCAGAGA 
TTCATATCTG CTTCCAATCA TGTTGAATTC ACAAAGTGTG TCAAAACAAA AGTTGTTGCA 
CCTTTATTGA TATCACACAC CTCAACCGAA TTGGGCGTAG TAAACCACTT GGATTTATTT 
GGTTGTGAGT ATTTGACTGA TAAGAATCTG CTAGCATATC TGGACATACT ACAACACCTG 
TCAAGTTACA TGAAGAGGAC CATTTTTCAT TCGCTTTTGT TATATTATGC TTCCAAAGCT 
TTTTTATTTT GGATAATGGC AAGGCCAAAA GAATACGTCA AAATTTATAA CAATCTAATA 
TCATCAGATT ATAATAGTCC GTCTTCTTCA TCTGATAATG GTGGTTCGAA TAATTCTGAT 
AAAACGTCTA TATCCCAACT AGTCTCACTG TTATTCGATG ACGTTTATTC CACTTTTAGT 
GTATCATCAT TATTAACAAA TGTCAATAAT GACCACCACT ACCATCTTCA TCATTCATCT 
TCTTCATCAA AGACGACCAA CACTAATAGT CCAAACTCTA TATCAAAAAC GTCGATAAAG 
CAGTCGAGTG TGAATGCTTC TGGCAATGTT TCTCCGTCTC AGTTTTCTAC TGGGAATGAT 
GCATCGCCTA CTTCCCCTAT GGCATCATTG AGTTCACCCT TAAACACGAA CATCCTAGGG 
TATCCGTTAT CTCCAATCAC TTCAACACTA GGACAGGCGA ATACTTCCAC ATCGACTACG 
GCTGCAACTA CCAAAACAGA TGCAGATACG CCCTCTACTA TGAATACTAA CAACAATAAT 
AACAATAACA ACAGCGCTAA TCTTAATAAT ATTCCACAAC GCATATTTTC CTTAGATGAC 
ATTTCATCCT TTAACTCGAG TAGAAAATCA CTCAATCTAG ATGATAGTAA CTCCTTGTTT 
CTTTGGGATA CTTCTCAGCA TTCTAATGCA TCGATGACAA ATACAAATAT GCATGCAGGA 
GTTAATAATT CTCAGTCTCA GAACGATCAG TCTTCTTTAA ACTATATGGA AAATATTATG 
GAGCTGTATT CCAACTATAC CGGATCAGAA CTATCCTCCC ATACTGCCAT ATTAAGGTTT 
TTGGTGGTTC TGACCTTATT AGACAGTGAA GTATATGATG AGATGAACTC AAATTCGTAT 
AGAAAAATTT CGGAACCGAT AATGAATATT AATCCGAAGG ACTCTAATAC TTCAAGTTGG 
GGCTCAGCAT CCAAAAACCC AAGTATCAGG CACCTCACCC ATGGCTTAAA AAAACTTACT 
TTACAGCAAG GCAGGAAACG TAACGTTAAA TTTTTGACAT ATTTGATTAG AAATTTGAAT 
GGGGGACAGT TCGTTTCAGA TGTTTCCTTG ATTGACTCTA TCAGGTCCAT TCTATTCTTA 
ATGACAATGA CGTCTTCTAT ATCCCAAATC GATTCAAATA TTGCTTCTGT TATTTTTTCG 
AAGAGATTCT ACAACTTGTT GGGTCAAAAT TTAGAGGTCG GCACCAATTG GAATTCTGCC 
ACTGCAAATA CTTTTATTTC TCATTGTGTT GAAAGGAATC CCCTTACACA TAGGCGTTTA 
CAATTAGAGT TTTTTGCAAG CGGTTTACAG CTGGATTCTG ATTTATTTTT AAGACATTTA 
CAACTGGAAA AAGAACTCAA TCACATAGAC CTTCCCAAAA TATCGTTATA CACTGAAGGA 
TTTAGGGTAT TTTTTCACCT AGTAAGCACC AAAAAACTTC ATGAGGATAT TGCAGAAAAA 
ACCTCCTCTG TGTTAAAGAG ACTTTTCTGC ATAATTGCTG ATATTTTGTT GAAAGCAACG 
CCTTATTTTG ACGATAATGT AACCAAGATT ATTGCTTCTA TATTGGATGG GCATATTTTA 
GATCAATTTG ACGCTGCGCG AACACTTTCT AATGATGATC ATGTCAGTTT TGATGCTGCC 
ACAAGCGTTT ACACTGAGCC AACCGAAATT ATTCATAACT CATCGGATGC CTCGTTAGTC 
TCTTCACTTT CCCAATCACC CTTATCAATT AACTCAGGAA GCAATATCAC CAATACGCGC 
ACCTGGGATA TTCAATCAAT CTTACCAACC TTATCGAACA GATCAAGTGC TTCTGATTTG 
AGCTTGTCTA ACATTTTGAC TAATCCGTTG GAGGCACAAC AAAATAATAA TGCAAACTTG 
TTAGCCCATA GATTATCTGG GGTTCCTACT ACTAAGAGAT ACGCTTCACC GAACGACTCT 
GAAAGATCAC GACAAAGTCC ATATTCTTCT CCGCCGCAAT TGCAACAAAG TGACTTGCCT 
TCTCCGCTCT CAGTCCTCTC GTCAAGTGCA GGATTTTCTT CTAATCATTC GATTACGGCA 
ACCCCAACTA TTTTGAAAAA CATCAAATCT CCAAAACCAA ACAAAACAAA AAAAATTGCT 
GATGATAAAC AATTGAAACA GCCTTCTTAT TCAAGAGTAA TACTGAGTGA CAATGATGAA 
GCAAGAAAGA TTATGATGAA CATTTTCAGC ATTTTCAAAA GAATGACCAA CTGGTTTATA 
CGCCCAGATG CTAATACAGA ATTCCCGAAG ACTTTTACGG ATATTATAAA ACCACTTTTT 
GTCTCTATAT TGGATTCTAA TCAAAGACTA CAAGTTACAG CGCGGGCTTT TATTGAAATC 
CCATTAAGTT ATATAGCTAC TTTTGAAGAC ATTGATAATG ATCTTGACCC AAGAGTACTG 
AATGACCATT ATTTGTTATG TACATATGCC GTTACTTTGT TTGCTTCATC ATTGTTTGAT 
TTGAAGTTAG AAAATGCGAA GAGAGAGATG CTACTAGACA TTATTGTTAA ATTTCAACGA 
GTCCGTTCTT ATTTATCAAA TTTAGCAGAA AAACACAACC TAGTCCAGGC AATAATTACG 
ACGGAGAGGT TGACGCTGCC ATTATTAGTT GGTGCTGTAG GAAGTGGAAT TTTCATTTCA 
TTATACTGCA GTCGTGGAAA TACGCCACGC TTAATAAAAA TTTCATGTTG TGAATTTCTA 
CGATCCTTGA GATTTTATCA AAAATACGTA GGCGCTTTGG ATCAATATTC CATTTACAAT 
ATTGATTTCA TAGATGCTAT GGCCCAGGAC AATTTCACTG CCTCAGGATC AGTGGCTTTG 
CAACGGCGTC TAAGAAATAA TATTTTAACT TATATCAAAG GATCCGACTC AATCCTTTTG 
GATTCAATGG ACGTGATTTA CAAGAAGTGG TTTTACTTCA GCTGTTCGAA ATCAGTTACG 
CAAGAGGAAC TAGTAGATTT TAGAAGCTTG GCAGGCATTC TAGCTTCTAT GTCAGGTATT 
CTGTCTGATA TGCAAGAGTT GGAAAAAAGC AAGAGCGCTC CAGATAATGA AGGAGACAGC 
TTATCATTTG AGTCACGAAA TCCCGCTTAT GAGGTGCACA AAAGTCTTAA ACTCGAGTTA 
ACGAAAAAAA TGAATTTCTT TATTTCAAAA CAATGTCAAT GGTTGAATAA TCCAAATCTA 
TTAACAAGAG AAAATTCGAG AGATATATTA AGTATTGAGT TGCATCCTCT ATCTTTTAAC 
TTATTGTTTA ACAACCTAGG ACTGAAAATA GATGAACTGA TGTCAATTGA TCTTTCAAAG 
TCACATGAGG ATTCATCGTT TGTTTTACTA GAGCAGATAA TAATTATAAT AAGAACTATA 
CTAAAGAGGG ATGATGATGA AAAGATAATG CTACTCTTTT CGACGGACTT GCTTGATGCG 
GTCGATAAGT TGATCGAAAT AGTGGAGAAA ATTTCCATCA AGTCCTCCAA ATATTATAAG 
GGAATTATCC AAATGTCGAA AATGTTTAGA GCATTTGAGC ACTCTGAAAA GAACCTGGGC 
ATTTCAAATC ATTTCCATTT AAAGAATAAA TGGTTGAAGT TAGTTATTGG TTGGTTCAAA 
CTATCTATTA ATAAGGATTA TGATTTTGAA AACCTGTCAA GACCATTAAG GGAAATGGAT 
TTGCAGAAAA GGGACGAAGA TTTTTTGTAT ATCGACACTT CTATTGAATC TGCAAAAGCA 
TTGGCTTACC TAACACATAA TGTTCCTTTA GAAATACCGC CTTCAAGCTC AAAAGAAGAT 
TGGAACAGAT CTTCTACAGT ATCATTTGGC AATCACTTTA CTATTTTGTT AAAAGGTCTG 
GAGAAAAGCG CGGACCTGAA TCAGTTTCCA GTTTCATTAA GGCATAAGAT CAGTATACTT 
AATGAAAATG TAATAATAGC GCTAACGAAC TTATCTAATG CCAATGTCAA CGTTTCTTTA 
AAATTCACTT TACCAATGGG TTATTCTCCA AATAAAGATA TCAGAATCGC CTTTTTAAGA 
GTTTTCATCG ACATAGTAAC CAACTATCCA GTTAACCCTG AGAAACATGA AATGGATAAA 
ATGCTAGCTA TAGACGACTT CCTGAAATAT ATAATCAAGA ACCCAATATT AGCATTTTTC 
GGAAGTTTAG CGTGTTCTCC TGCTGATGTT GATTTATATG CTGGTGGATT CTTAAACGCC 
TTTGACACTA GAAATGCGTC TCATATCCTT GTTACTGAGC TCCTTAAACA AGAAATCAAA 
CGGGCCGCAA GATCAGACGA TATTCTCAGA AGAAATAGTT GTGCAACAAG GGCTTTGTCA 
CTTTACACTA GATCTAGAGG TAACAAATAT TTGATAAAAA CTTTGAGACC CGTTTTGCAA 
GGGATAGTGG ATAACAAGGA GTCTTTTGAA ATTGATAAGA TGAAACCAGG ATCCGAAAAC 
TCCGAAAAGA TGTTAGACTT ATTTGAGAAG TACATGACAA GATTAATTGA CGCAATTACA 
AGTTCTATTG ATGATTTCCC AATAGAATTA GTTGATATCT GTAAAACAAT TTACAATGCT 
GCTAGTGTAA ATTTTCCAGA ATACGCATAT ATTGCCGTTG GGTCATTCGT TTTCTTGAGG 
TTTATCGGGC CTGCTTTAGT TAGTCCTGAT TCGGAAAATA TCATTATTGT TACGCACGCC 
CATGACAGAA AGCCCTTTAT TACACTAGCT AAAGTTATTC AAAGTTTAGC TAATGGCAGG 
GAAAATATAT TCAAGAAAGA TATCTTAGTT TCAAAAGAAG AGTTTTTGAA AACCTGTAGT 
GATAAAATAT TCAATTTTTT GTCTGAATTG TGCAAGATAC CGACTAACAA TTTCACCGTC 
AATGTAAGAG AAGATCCGAC ACCAATAAGC TTTGACTACT CATTTTTGCA TAAATTCTTT 
TACCTCAATG AGTTTACCAT AAGAAAAGAA ATTATTAATG AATCCAAATT ACCAGGGGAG 
TTCAGCTTTT TGAAAAATAC TGTTATGCTC AACGACAAAA TTCTTGGTGT ATTGGGACAA 
CCTAGCATGG AAATAAAAAA TGAAATTCCT CCTTTTGTAG TCGAGAATCG GGAAAAATAT 
CCTTCATTGT ATGAATTCAT GAGTCGCTAT GCCTTCAAAA AAGTGGACAT GAAAGAAGAA 
GAAGAGGATA ATGCGCCATT TGTACATGAA GCAATGACAT TGGATGGCAT ACAAATCATT 
GTCGTAACTT TTACCAATTG CGAGTACAAT AATTTTGTAA TGGACTCACT GGTCTATAAA 
GTTCTGCAGA TATATGCAAG AATGTGGTGC TCTAAACATT ATGTAGTTAT CGATTGTACC 
ACCTTTTATG GGGGTAAGGC TAATTTCCAA AAATTGACTA CTCTATTTTT CAGTTTGATA 
CCAGAGCAAG CATCAAGTAA TTGTATGGGA TGTTATTACT TCAACGTCAA CAAATCATTT 
ATGGACCAAT GGGCCTCATC ATATACTGTA GAAAATCCGT ACTTGGTCAC TACAATTCCC 
CGTTGTTTCA TCAACAGCAA TACTGACCAA AGTTTGATAA AGTCCTTAGG ATTGAGTGGT 
AGGAGTTTGG AAGTTTTGAA AGATGTAAGA GTTACTTTGC ATGATATTAC CCTTTATGAC 
AAGGAAAAAA AGAAGTTTTG TCCCGTGTCC TTGAAGATAG GAAACAAATA CTTCCAAGTT 
TTACATGAGA TTCCGCAGTT GTACAAGGTT ACCGTATCAA ACAGGACATT CAGCATCAAA 
TTCAACAATG TTTACAAGAT ATCAAATTTA ATTTCAGTCG ATGTCTCTAA CACCACAGGC 
GTTTCCTCGG AATTTACGTT AAGTCTTGAT AATGAAGAAA AGTTGGTATT TTGCAGTCCG 
AAGTACCTAG AAATTGTGAA AATGTTTTAT TATGCCCAGT TAAAGATGGA AGAAGACTTT 
GGTACGGATT TTTCGAACGA TATTTCATTT TCAACATCCT CTTCAGCAGT TAATGCTTCT 
TACTGCAATG TTAAAGAAGT TGGTGAAATT ATATCACATT TGTCATTGGT GATCCTTGTA 
GGTTTATTCA ATGAGGATGA TCTCGTCAAA AACATATCAT ACAACCTTCT CGTGGCAACG 
CAAGAAGCAT TTAATTTAGA TTTTGGGACA AGGCTTCACA AATCCCCAGA GACATATGTA 
CCCGATGATA CCACCACGTT CTTGGCCCTA ATTTTCAAGG CTTTTTCAGA ATCTTCAACG 
GAACTAACTC CATATATATG GAAATATATG CTGGATGGCC TTGAAAACGA CGTGATTCCT 
CAAGAACATA TTCCTACGGT TGTCTGTTCA TTGTCATACT GGGTACCAAA CTTATATGAA 
CATGTATATT TGGCAAATGA CGAAGAGGGA CCAGAGGCGA TTTCACGTAT AATCTATAGC 
TTAATCAGGT TGACGGTCAA AGAGCCAAAT TTCACGACAG CTTACCTTCA ACAGATTTGG 
TTTTTACTGG CATTGGATGG TCGTCTCACG AACGTGATAG TTGAAGAAAT AGTAAGTCAT 
GCGCTGGATA GAGATTCAGA AAACAGAGAC TGGATGAAAG CTGTGTCAAT ACTAACCAGT 
TTTCCAACGA CAGAGATTGC TTGTCAGGTA ATAGAGAAGC TAATAAATAT GATCAAATCT 
TTTCTACCTT CTCTAGCAGT TGAGGCTTCC GCACACAGTT GGTCTGAGCT TACTATTTTA 
TCAAAAATTA GTGTGTCAAT TTTCTTTGAA TCACCCTTAC TTTCCCAGAT GTATTTACCG 
GAGATTCTTT TCGCTGTGTC TCTGTTAATT GATGTCGGTC CTTCGGAAAT AAGAGTCTCA 
TTGTACGAGT TGTTGATGAA TGTTTGTCAT TCTTTAACCA ACAATGAGTC CTTACCTGAA 
AGGAATAGGA AAAATTTGGA TATCGTCTGT GCAACATTCG CACGTCAAAA GTTGAACTTT 
ATCTCCGGTT TTAGCCAAGA AAAAGGTAGA GTTTTACCAA ATTTTGCCGC TTCCTCCTTC 
TCCAGTAAAT TCGGAACATT AGATCTCTTC ACTAAAAACA TTATGCTATT GATGGAATAT 
GGTTCTATTT CAGAGGGTGC ACAATGGGAG GCAAAATATA AGAAATATTT GATGGATGCG 
ATTTTTGGCC ATCGGTCGTT CTTCTCTGCG AGAGCTATGA TGATTCTAGG TATAATGAGT 
AAGTCGCACA CGTCCCTTTT CCTTTGTAAA GAACTTTTAG TTGAAACCAT GAAGGTCTTC 
GCAGAGCCAG TTGTGGATGA TGAACAAATG TTCATCATTA TAGCTCATGT CTTTACTTAC 
AGCAAAATTG TCGAAGGGTT AGATCCTTCT TCAGAATTAA TGAAAGAGCT ATTTTGGCTT 
GCTACAATAT GTGTTGAATC CCCTCATCCT TTACTCTTTG AAGGTGGTCT CCTGTTCATG 
GTAAATTGTT TGAAGCGACT GTACACGGTC CATCTTCAAC TTGGATTCGA TGGCAAATCG 
CTAGCCAAAA AATTAATGGA ATCTAGAAAT TTTGCTGCTA CGCTTTTGGC TAAGTTAGAG 
TCATACAATG GATGCATATG GAACGAAGAT AATTTTCCTC ATATTATTTT AGGTTTCATT 
GCAAACGGTT TATCCATTCC TGTCGTAAAA GGAGCCGCAT TAGATTGTCT ACAGGCCCTT 
TTCAAGAATA CATATTACGA AAGAAAGTCC AACCCAAAAT CCTCCGATTA TCTTTGTTAC 
CTTTTCTTAC TCCATTTGGT CTTAAGTCCT GAACAACTTT CTACCTTGTT ACTTGAAGTC 
GGCTTCGAAG ATGAACTGGT ACCTTTAAAT AATACACTAA AAGTGCCACT TACTTTGATC 
AACTGGCTAA GTTCAGACTC AGATAAATCT AATATAGTCT TATACCAAGG AGCACTTTTG 
TTTAGCTGTG TTATGTCAGA CGAACCATGT AAATTCCGTT TTGCTCTATT GATGAGGTAT 
TTGCTCAAAG TCAACCCTAT TTGTGTATTC AGGTTCTATA CGCTGACTAG AAAGGAATTC 
AGGAGGTTAT CAACCCTAGA ACAATCATCT GAAGCGGTTG CTGTCTCTTT TGAATTGATT 
GGGATGCTTG TTACACACAG TGAGTTTAAT TACCTAGAGG AATTTAATGA TGAAATGGTC 
GAACTTTTAA AAAAGAGAGG CTTGTCAGTT GTGAAGCCTC TGGATATTTT TGATCAGGAA 
CATATAGAAA AGTTAAAAGG AGAGGGTGAA CATCAAGTGG CAATTTATGA GAGAAAAAGA 
TTAGCAACAA TGATACTGGC AAGAATGTCG TGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009698.3
CDS	1..9279
Translation	MNQSDPQDKKNFPMEYSLTKHLFFDRLLLVLPIESNLKTYADVEADSVFNSCRSIILNIAITKDLNPIIENTLGLIDLIVQDEEITSDNITDDIAHSILVLLRLLSDVFEYYWDQNNDFKKIRNDNYKPGFSSHRPNFHTSRPKHTRINPALATMLLCKISKLKFNTRTLKVLQNMSHHLSGSATISKSSILPDSQEFLQKRNYPAYTEKIDLTIDYIQRFISASNHVEFTKCVKTKVVAPLLISHTSTELGVVNHLDLFGCEYLTDKNLLAYLDILQHLSSYMKRTIFHSLLLYYASKAFLFWIMARPKEYVKIYNNLISSDYNSPSSSSDNGGSNNSDKTSISQLVSLLFDDVYSTFSVSSLLTNVNNDHHYHLHHSSSSSKTTNTNSPNSISKTSIKQSSVNASGNVSPSQFSTGNDASPTSPMASLSSPLNTNILGYPLSPITSTLGQANTSTSTTAATTKTDADTPSTMNTNNNNNNNNSANLNNIPQRIFSLDDISSFNSSRKSLNLDDSNSLFLWDTSQHSNASMTNTNMHAGVNNSQSQNDQSSLNYMENIMELYSNYTGSELSSHTAILRFLVVLTLLDSEVYDEMNSNSYRKISEPIMNINPKDSNTSSWGSASKNPSIRHLTHGLKKLTLQQGRKRNVKFLTYLIRNLNGGQFVSDVSLIDSIRSILFLMTMTSSISQIDSNIASVIFSKRFYNLLGQNLEVGTNWNSATANTFISHCVERNPLTHRRLQLEFFASGLQLDSDLFLRHLQLEKELNHIDLPKISLYTEGFRVFFHLVSTKKLHEDIAEKTSSVLKRLFCIIADILLKATPYFDDNVTKIIASILDGHILDQFDAARTLSNDDHVSFDAATSVYTEPTEIIHNSSDASLVSSLSQSPLSINSGSNITNTRTWDIQSILPTLSNRSSASDLSLSNILTNPLEAQQNNNANLLAHRLSGVPTTKRYASPNDSERSRQSPYSSPPQLQQSDLPSPLSVLSSSAGFSSNHSITATPTILKNIKSPKPNKTKKIADDKQLKQPSYSRVILSDNDEARKIMMNIFSIFKRMTNWFIRPDANTEFPKTFTDIIKPLFVSILDSNQRLQVTARAFIEIPLSYIATFEDIDNDLDPRVLNDHYLLCTYAVTLFASSLFDLKLENAKREMLLDIIVKFQRVRSYLSNLAEKHNLVQAIITTERLTLPLLVGAVGSGIFISLYCSRGNTPRLIKISCCEFLRSLRFYQKYVGALDQYSIYNIDFIDAMAQDNFTASGSVALQRRLRNNILTYIKGSDSILLDSMDVIYKKWFYFSCSKSVTQEELVDFRSLAGILASMSGILSDMQELEKSKSAPDNEGDSLSFESRNPAYEVHKSLKLELTKKMNFFISKQCQWLNNPNLLTRENSRDILSIELHPLSFNLLFNNLGLKIDELMSIDLSKSHEDSSFVLLEQIIIIIRTILKRDDDEKIMLLFSTDLLDAVDKLIEIVEKISIKSSKYYKGIIQMSKMFRAFEHSEKNLGISNHFHLKNKWLKLVIGWFKLSINKDYDFENLSRPLREMDLQKRDEDFLYIDTSIESAKALAYLTHNVPLEIPPSSSKEDWNRSSTVSFGNHFTILLKGLEKSADLNQFPVSLRHKISILNENVIIALTNLSNANVNVSLKFTLPMGYSPNKDIRIAFLRVFIDIVTNYPVNPEKHEMDKMLAIDDFLKYIIKNPILAFFGSLACSPADVDLYAGGFLNAFDTRNASHILVTELLKQEIKRAARSDDILRRNSCATRALSLYTRSRGNKYLIKTLRPVLQGIVDNKESFEIDKMKPGSENSEKMLDLFEKYMTRLIDAITSSIDDFPIELVDICKTIYNAASVNFPEYAYIAVGSFVFLRFIGPALVSPDSENIIIVTHAHDRKPFITLAKVIQSLANGRENIFKKDILVSKEEFLKTCSDKIFNFLSELCKIPTNNFTVNVREDPTPISFDYSFLHKFFYLNEFTIRKEIINESKLPGEFSFLKNTVMLNDKILGVLGQPSMEIKNEIPPFVVENREKYPSLYEFMSRYAFKKVDMKEEEEDNAPFVHEAMTLDGIQIIVVTFTNCEYNNFVMDSLVYKVLQIYARMWCSKHYVVIDCTTFYGGKANFQKLTTLFFSLIPEQASSNCMGCYYFNVNKSFMDQWASSYTVENPYLVTTIPRCFINSNTDQSLIKSLGLSGRSLEVLKDVRVTLHDITLYDKEKKKFCPVSLKIGNKYFQVLHEIPQLYKVTVSNRTFSIKFNNVYKISNLISVDVSNTTGVSSEFTLSLDNEEKLVFCSPKYLEIVKMFYYAQLKMEEDFGTDFSNDISFSTSSSAVNASYCNVKEVGEIISHLSLVILVGLFNEDDLVKNISYNLLVATQEAFNLDFGTRLHKSPETYVPDDTTTFLALIFKAFSESSTELTPYIWKYMLDGLENDVIPQEHIPTVVCSLSYWVPNLYEHVYLANDEEGPEAISRIIYSLIRLTVKEPNFTTAYLQQIWFLLALDGRLTNVIVEEIVSHALDRDSENRDWMKAVSILTSFPTTEIACQVIEKLINMIKSFLPSLAVEASAHSWSELTILSKISVSIFFESPLLSQMYLPEILFAVSLLIDVGPSEIRVSLYELLMNVCHSLTNNESLPERNRKNLDIVCATFARQKLNFISGFSQEKGRVLPNFAASSFSSKFGTLDLFTKNIMLLMEYGSISEGAQWEAKYKKYLMDAIFGHRSFFSARAMMILGIMSKSHTSLFLCKELLVETMKVFAEPVVDDEQMFIIIAHVFTYSKIVEGLDPSSELMKELFWLATICVESPHPLLFEGGLLFMVNCLKRLYTVHLQLGFDGKSLAKKLMESRNFAATLLAKLESYNGCIWNEDNFPHIILGFIANGLSIPVVKGAALDCLQALFKNTYYERKSNPKSSDYLCYLFLLHLVLSPEQLSTLLLEVGFEDELVPLNNTLKVPLTLINWLSSDSDKSNIVLYQGALLFSCVMSDEPCKFRFALLMRYLLKVNPICVFRFYTLTRKEFRRLSTLEQSSEAVAVSFELIGMLVTHSEFNYLEEFNDEMVELLKKRGLSVVKPLDIFDQEHIEKLKGEGEHQVAIYERKRLATMILARMSCS

Target ORF information:

RefSeq Version	NM_001178488.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C GTPase-activating protein IRA1 (IRA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178488.3

ORF Insert Sequence:

ATGAATCAAA GCGATCCGCA AGACAAAAAA AATTTCCCAA TGGAATACTC TTTGACCAAG 
CATCTTTTTT TTGATAGGCT TCTACTTGTT CTTCCCATAG AATCTAATTT GAAAACATAT 
GCTGATGTGG AGGCAGATTC AGTTTTCAAT TCGTGTCGGT CCATCATTTT GAATATAGCC 
ATCACTAAGG ACTTGAACCC GATTATCGAA AACACATTAG GTTTAATTGA CTTGATTGTG 
CAAGATGAAG AAATTACGTC TGACAATATT ACAGATGATA TTGCCCATTC TATATTGGTT 
CTTTTGAGAT TACTGAGTGA TGTTTTTGAG TATTACTGGG ATCAAAACAA TGACTTCAAG 
AAAATTAGAA ACGATAATTA CAAACCGGGA TTTTCAAGTC ACAGGCCAAA CTTCCATACA 
TCTAGGCCAA AGCACACGAG AATCAATCCA GCTTTGGCGA CGATGTTACT ATGTAAAATT 
TCTAAGCTGA AGTTCAATAC AAGAACTTTA AAGGTTTTAC AGAACATGTC TCACCATCTT 
TCTGGCAGCG CTACTATCTC AAAATCGAGT ATTTTACCCG ATTCACAGGA ATTTTTACAA 
AAGAGAAACT ATCCAGCATA TACCGAGAAA ATAGATTTAA CAATAGATTA TATCCAGAGA 
TTCATATCTG CTTCCAATCA TGTTGAATTC ACAAAGTGTG TCAAAACAAA AGTTGTTGCA 
CCTTTATTGA TATCACACAC CTCAACCGAA TTGGGCGTAG TAAACCACTT GGATTTATTT 
GGTTGTGAGT ATTTGACTGA TAAGAATCTG CTAGCATATC TGGACATACT ACAACACCTG 
TCAAGTTACA TGAAGAGGAC CATTTTTCAT TCGCTTTTGT TATATTATGC TTCCAAAGCT 
TTTTTATTTT GGATAATGGC AAGGCCAAAA GAATACGTCA AAATTTATAA CAATCTAATA 
TCATCAGATT ATAATAGTCC GTCTTCTTCA TCTGATAATG GTGGTTCGAA TAATTCTGAT 
AAAACGTCTA TATCCCAACT AGTCTCACTG TTATTCGATG ACGTTTATTC CACTTTTAGT 
GTATCATCAT TATTAACAAA TGTCAATAAT GACCACCACT ACCATCTTCA TCATTCATCT 
TCTTCATCAA AGACGACCAA CACTAATAGT CCAAACTCTA TATCAAAAAC GTCGATAAAG 
CAGTCGAGTG TGAATGCTTC TGGCAATGTT TCTCCGTCTC AGTTTTCTAC TGGGAATGAT 
GCATCGCCTA CTTCCCCTAT GGCATCATTG AGTTCACCCT TAAACACGAA CATCCTAGGG 
TATCCGTTAT CTCCAATCAC TTCAACACTA GGACAGGCGA ATACTTCCAC ATCGACTACG 
GCTGCAACTA CCAAAACAGA TGCAGATACG CCCTCTACTA TGAATACTAA CAACAATAAT 
AACAATAACA ACAGCGCTAA TCTTAATAAT ATTCCACAAC GCATATTTTC CTTAGATGAC 
ATTTCATCCT TTAACTCGAG TAGAAAATCA CTCAATCTAG ATGATAGTAA CTCCTTGTTT 
CTTTGGGATA CTTCTCAGCA TTCTAATGCA TCGATGACAA ATACAAATAT GCATGCAGGA 
GTTAATAATT CTCAGTCTCA GAACGATCAG TCTTCTTTAA ACTATATGGA AAATATTATG 
GAGCTGTATT CCAACTATAC CGGATCAGAA CTATCCTCCC ATACTGCCAT ATTAAGGTTT 
TTGGTGGTTC TGACCTTATT AGACAGTGAA GTATATGATG AGATGAACTC AAATTCGTAT 
AGAAAAATTT CGGAACCGAT AATGAATATT AATCCGAAGG ACTCTAATAC TTCAAGTTGG 
GGCTCAGCAT CCAAAAACCC AAGTATCAGG CACCTCACCC ATGGCTTAAA AAAACTTACT 
TTACAGCAAG GCAGGAAACG TAACGTTAAA TTTTTGACAT ATTTGATTAG AAATTTGAAT 
GGGGGACAGT TCGTTTCAGA TGTTTCCTTG ATTGACTCTA TCAGGTCCAT TCTATTCTTA 
ATGACAATGA CGTCTTCTAT ATCCCAAATC GATTCAAATA TTGCTTCTGT TATTTTTTCG 
AAGAGATTCT ACAACTTGTT GGGTCAAAAT TTAGAGGTCG GCACCAATTG GAATTCTGCC 
ACTGCAAATA CTTTTATTTC TCATTGTGTT GAAAGGAATC CCCTTACACA TAGGCGTTTA 
CAATTAGAGT TTTTTGCAAG CGGTTTACAG CTGGATTCTG ATTTATTTTT AAGACATTTA 
CAACTGGAAA AAGAACTCAA TCACATAGAC CTTCCCAAAA TATCGTTATA CACTGAAGGA 
TTTAGGGTAT TTTTTCACCT AGTAAGCACC AAAAAACTTC ATGAGGATAT TGCAGAAAAA 
ACCTCCTCTG TGTTAAAGAG ACTTTTCTGC ATAATTGCTG ATATTTTGTT GAAAGCAACG 
CCTTATTTTG ACGATAATGT AACCAAGATT ATTGCTTCTA TATTGGATGG GCATATTTTA 
GATCAATTTG ACGCTGCGCG AACACTTTCT AATGATGATC ATGTCAGTTT TGATGCTGCC 
ACAAGCGTTT ACACTGAGCC AACCGAAATT ATTCATAACT CATCGGATGC CTCGTTAGTC 
TCTTCACTTT CCCAATCACC CTTATCAATT AACTCAGGAA GCAATATCAC CAATACGCGC 
ACCTGGGATA TTCAATCAAT CTTACCAACC TTATCGAACA GATCAAGTGC TTCTGATTTG 
AGCTTGTCTA ACATTTTGAC TAATCCGTTG GAGGCACAAC AAAATAATAA TGCAAACTTG 
TTAGCCCATA GATTATCTGG GGTTCCTACT ACTAAGAGAT ACGCTTCACC GAACGACTCT 
GAAAGATCAC GACAAAGTCC ATATTCTTCT CCGCCGCAAT TGCAACAAAG TGACTTGCCT 
TCTCCGCTCT CAGTCCTCTC GTCAAGTGCA GGATTTTCTT CTAATCATTC GATTACGGCA 
ACCCCAACTA TTTTGAAAAA CATCAAATCT CCAAAACCAA ACAAAACAAA AAAAATTGCT 
GATGATAAAC AATTGAAACA GCCTTCTTAT TCAAGAGTAA TACTGAGTGA CAATGATGAA 
GCAAGAAAGA TTATGATGAA CATTTTCAGC ATTTTCAAAA GAATGACCAA CTGGTTTATA 
CGCCCAGATG CTAATACAGA ATTCCCGAAG ACTTTTACGG ATATTATAAA ACCACTTTTT 
GTCTCTATAT TGGATTCTAA TCAAAGACTA CAAGTTACAG CGCGGGCTTT TATTGAAATC 
CCATTAAGTT ATATAGCTAC TTTTGAAGAC ATTGATAATG ATCTTGACCC AAGAGTACTG 
AATGACCATT ATTTGTTATG TACATATGCC GTTACTTTGT TTGCTTCATC ATTGTTTGAT 
TTGAAGTTAG AAAATGCGAA GAGAGAGATG CTACTAGACA TTATTGTTAA ATTTCAACGA 
GTCCGTTCTT ATTTATCAAA TTTAGCAGAA AAACACAACC TAGTCCAGGC AATAATTACG 
ACGGAGAGGT TGACGCTGCC ATTATTAGTT GGTGCTGTAG GAAGTGGAAT TTTCATTTCA 
TTATACTGCA GTCGTGGAAA TACGCCACGC TTAATAAAAA TTTCATGTTG TGAATTTCTA 
CGATCCTTGA GATTTTATCA AAAATACGTA GGCGCTTTGG ATCAATATTC CATTTACAAT 
ATTGATTTCA TAGATGCTAT GGCCCAGGAC AATTTCACTG CCTCAGGATC AGTGGCTTTG 
CAACGGCGTC TAAGAAATAA TATTTTAACT TATATCAAAG GATCCGACTC AATCCTTTTG 
GATTCAATGG ACGTGATTTA CAAGAAGTGG TTTTACTTCA GCTGTTCGAA ATCAGTTACG 
CAAGAGGAAC TAGTAGATTT TAGAAGCTTG GCAGGCATTC TAGCTTCTAT GTCAGGTATT 
CTGTCTGATA TGCAAGAGTT GGAAAAAAGC AAGAGCGCTC CAGATAATGA AGGAGACAGC 
TTATCATTTG AGTCACGAAA TCCCGCTTAT GAGGTGCACA AAAGTCTTAA ACTCGAGTTA 
ACGAAAAAAA TGAATTTCTT TATTTCAAAA CAATGTCAAT GGTTGAATAA TCCAAATCTA 
TTAACAAGAG AAAATTCGAG AGATATATTA AGTATTGAGT TGCATCCTCT ATCTTTTAAC 
TTATTGTTTA ACAACCTAGG ACTGAAAATA GATGAACTGA TGTCAATTGA TCTTTCAAAG 
TCACATGAGG ATTCATCGTT TGTTTTACTA GAGCAGATAA TAATTATAAT AAGAACTATA 
CTAAAGAGGG ATGATGATGA AAAGATAATG CTACTCTTTT CGACGGACTT GCTTGATGCG 
GTCGATAAGT TGATCGAAAT AGTGGAGAAA ATTTCCATCA AGTCCTCCAA ATATTATAAG 
GGAATTATCC AAATGTCGAA AATGTTTAGA GCATTTGAGC ACTCTGAAAA GAACCTGGGC 
ATTTCAAATC ATTTCCATTT AAAGAATAAA TGGTTGAAGT TAGTTATTGG TTGGTTCAAA 
CTATCTATTA ATAAGGATTA TGATTTTGAA AACCTGTCAA GACCATTAAG GGAAATGGAT 
TTGCAGAAAA GGGACGAAGA TTTTTTGTAT ATCGACACTT CTATTGAATC TGCAAAAGCA 
TTGGCTTACC TAACACATAA TGTTCCTTTA GAAATACCGC CTTCAAGCTC AAAAGAAGAT 
TGGAACAGAT CTTCTACAGT ATCATTTGGC AATCACTTTA CTATTTTGTT AAAAGGTCTG 
GAGAAAAGCG CGGACCTGAA TCAGTTTCCA GTTTCATTAA GGCATAAGAT CAGTATACTT 
AATGAAAATG TAATAATAGC GCTAACGAAC TTATCTAATG CCAATGTCAA CGTTTCTTTA 
AAATTCACTT TACCAATGGG TTATTCTCCA AATAAAGATA TCAGAATCGC CTTTTTAAGA 
GTTTTCATCG ACATAGTAAC CAACTATCCA GTTAACCCTG AGAAACATGA AATGGATAAA 
ATGCTAGCTA TAGACGACTT CCTGAAATAT ATAATCAAGA ACCCAATATT AGCATTTTTC 
GGAAGTTTAG CGTGTTCTCC TGCTGATGTT GATTTATATG CTGGTGGATT CTTAAACGCC 
TTTGACACTA GAAATGCGTC TCATATCCTT GTTACTGAGC TCCTTAAACA AGAAATCAAA 
CGGGCCGCAA GATCAGACGA TATTCTCAGA AGAAATAGTT GTGCAACAAG GGCTTTGTCA 
CTTTACACTA GATCTAGAGG TAACAAATAT TTGATAAAAA CTTTGAGACC CGTTTTGCAA 
GGGATAGTGG ATAACAAGGA GTCTTTTGAA ATTGATAAGA TGAAACCAGG ATCCGAAAAC 
TCCGAAAAGA TGTTAGACTT ATTTGAGAAG TACATGACAA GATTAATTGA CGCAATTACA 
AGTTCTATTG ATGATTTCCC AATAGAATTA GTTGATATCT GTAAAACAAT TTACAATGCT 
GCTAGTGTAA ATTTTCCAGA ATACGCATAT ATTGCCGTTG GGTCATTCGT TTTCTTGAGG 
TTTATCGGGC CTGCTTTAGT TAGTCCTGAT TCGGAAAATA TCATTATTGT TACGCACGCC 
CATGACAGAA AGCCCTTTAT TACACTAGCT AAAGTTATTC AAAGTTTAGC TAATGGCAGG 
GAAAATATAT TCAAGAAAGA TATCTTAGTT TCAAAAGAAG AGTTTTTGAA AACCTGTAGT 
GATAAAATAT TCAATTTTTT GTCTGAATTG TGCAAGATAC CGACTAACAA TTTCACCGTC 
AATGTAAGAG AAGATCCGAC ACCAATAAGC TTTGACTACT CATTTTTGCA TAAATTCTTT 
TACCTCAATG AGTTTACCAT AAGAAAAGAA ATTATTAATG AATCCAAATT ACCAGGGGAG 
TTCAGCTTTT TGAAAAATAC TGTTATGCTC AACGACAAAA TTCTTGGTGT ATTGGGACAA 
CCTAGCATGG AAATAAAAAA TGAAATTCCT CCTTTTGTAG TCGAGAATCG GGAAAAATAT 
CCTTCATTGT ATGAATTCAT GAGTCGCTAT GCCTTCAAAA AAGTGGACAT GAAAGAAGAA 
GAAGAGGATA ATGCGCCATT TGTACATGAA GCAATGACAT TGGATGGCAT ACAAATCATT 
GTCGTAACTT TTACCAATTG CGAGTACAAT AATTTTGTAA TGGACTCACT GGTCTATAAA 
GTTCTGCAGA TATATGCAAG AATGTGGTGC TCTAAACATT ATGTAGTTAT CGATTGTACC 
ACCTTTTATG GGGGTAAGGC TAATTTCCAA AAATTGACTA CTCTATTTTT CAGTTTGATA 
CCAGAGCAAG CATCAAGTAA TTGTATGGGA TGTTATTACT TCAACGTCAA CAAATCATTT 
ATGGACCAAT GGGCCTCATC ATATACTGTA GAAAATCCGT ACTTGGTCAC TACAATTCCC 
CGTTGTTTCA TCAACAGCAA TACTGACCAA AGTTTGATAA AGTCCTTAGG ATTGAGTGGT 
AGGAGTTTGG AAGTTTTGAA AGATGTAAGA GTTACTTTGC ATGATATTAC CCTTTATGAC 
AAGGAAAAAA AGAAGTTTTG TCCCGTGTCC TTGAAGATAG GAAACAAATA CTTCCAAGTT 
TTACATGAGA TTCCGCAGTT GTACAAGGTT ACCGTATCAA ACAGGACATT CAGCATCAAA 
TTCAACAATG TTTACAAGAT ATCAAATTTA ATTTCAGTCG ATGTCTCTAA CACCACAGGC 
GTTTCCTCGG AATTTACGTT AAGTCTTGAT AATGAAGAAA AGTTGGTATT TTGCAGTCCG 
AAGTACCTAG AAATTGTGAA AATGTTTTAT TATGCCCAGT TAAAGATGGA AGAAGACTTT 
GGTACGGATT TTTCGAACGA TATTTCATTT TCAACATCCT CTTCAGCAGT TAATGCTTCT 
TACTGCAATG TTAAAGAAGT TGGTGAAATT ATATCACATT TGTCATTGGT GATCCTTGTA 
GGTTTATTCA ATGAGGATGA TCTCGTCAAA AACATATCAT ACAACCTTCT CGTGGCAACG 
CAAGAAGCAT TTAATTTAGA TTTTGGGACA AGGCTTCACA AATCCCCAGA GACATATGTA 
CCCGATGATA CCACCACGTT CTTGGCCCTA ATTTTCAAGG CTTTTTCAGA ATCTTCAACG 
GAACTAACTC CATATATATG GAAATATATG CTGGATGGCC TTGAAAACGA CGTGATTCCT 
CAAGAACATA TTCCTACGGT TGTCTGTTCA TTGTCATACT GGGTACCAAA CTTATATGAA 
CATGTATATT TGGCAAATGA CGAAGAGGGA CCAGAGGCGA TTTCACGTAT AATCTATAGC 
TTAATCAGGT TGACGGTCAA AGAGCCAAAT TTCACGACAG CTTACCTTCA ACAGATTTGG 
TTTTTACTGG CATTGGATGG TCGTCTCACG AACGTGATAG TTGAAGAAAT AGTAAGTCAT 
GCGCTGGATA GAGATTCAGA AAACAGAGAC TGGATGAAAG CTGTGTCAAT ACTAACCAGT 
TTTCCAACGA CAGAGATTGC TTGTCAGGTA ATAGAGAAGC TAATAAATAT GATCAAATCT 
TTTCTACCTT CTCTAGCAGT TGAGGCTTCC GCACACAGTT GGTCTGAGCT TACTATTTTA 
TCAAAAATTA GTGTGTCAAT TTTCTTTGAA TCACCCTTAC TTTCCCAGAT GTATTTACCG 
GAGATTCTTT TCGCTGTGTC TCTGTTAATT GATGTCGGTC CTTCGGAAAT AAGAGTCTCA 
TTGTACGAGT TGTTGATGAA TGTTTGTCAT TCTTTAACCA ACAATGAGTC CTTACCTGAA 
AGGAATAGGA AAAATTTGGA TATCGTCTGT GCAACATTCG CACGTCAAAA GTTGAACTTT 
ATCTCCGGTT TTAGCCAAGA AAAAGGTAGA GTTTTACCAA ATTTTGCCGC TTCCTCCTTC 
TCCAGTAAAT TCGGAACATT AGATCTCTTC ACTAAAAACA TTATGCTATT GATGGAATAT 
GGTTCTATTT CAGAGGGTGC ACAATGGGAG GCAAAATATA AGAAATATTT GATGGATGCG 
ATTTTTGGCC ATCGGTCGTT CTTCTCTGCG AGAGCTATGA TGATTCTAGG TATAATGAGT 
AAGTCGCACA CGTCCCTTTT CCTTTGTAAA GAACTTTTAG TTGAAACCAT GAAGGTCTTC 
GCAGAGCCAG TTGTGGATGA TGAACAAATG TTCATCATTA TAGCTCATGT CTTTACTTAC 
AGCAAAATTG TCGAAGGGTT AGATCCTTCT TCAGAATTAA TGAAAGAGCT ATTTTGGCTT 
GCTACAATAT GTGTTGAATC CCCTCATCCT TTACTCTTTG AAGGTGGTCT CCTGTTCATG 
GTAAATTGTT TGAAGCGACT GTACACGGTC CATCTTCAAC TTGGATTCGA TGGCAAATCG 
CTAGCCAAAA AATTAATGGA ATCTAGAAAT TTTGCTGCTA CGCTTTTGGC TAAGTTAGAG 
TCATACAATG GATGCATATG GAACGAAGAT AATTTTCCTC ATATTATTTT AGGTTTCATT 
GCAAACGGTT TATCCATTCC TGTCGTAAAA GGAGCCGCAT TAGATTGTCT ACAGGCCCTT 
TTCAAGAATA CATATTACGA AAGAAAGTCC AACCCAAAAT CCTCCGATTA TCTTTGTTAC 
CTTTTCTTAC TCCATTTGGT CTTAAGTCCT GAACAACTTT CTACCTTGTT ACTTGAAGTC 
GGCTTCGAAG ATGAACTGGT ACCTTTAAAT AATACACTAA AAGTGCCACT TACTTTGATC 
AACTGGCTAA GTTCAGACTC AGATAAATCT AATATAGTCT TATACCAAGG AGCACTTTTG 
TTTAGCTGTG TTATGTCAGA CGAACCATGT AAATTCCGTT TTGCTCTATT GATGAGGTAT 
TTGCTCAAAG TCAACCCTAT TTGTGTATTC AGGTTCTATA CGCTGACTAG AAAGGAATTC 
AGGAGGTTAT CAACCCTAGA ACAATCATCT GAAGCGGTTG CTGTCTCTTT TGAATTGATT 
GGGATGCTTG TTACACACAG TGAGTTTAAT TACCTAGAGG AATTTAATGA TGAAATGGTC 
GAACTTTTAA AAAAGAGAGG CTTGTCAGTT GTGAAGCCTC TGGATATTTT TGATCAGGAA 
CATATAGAAA AGTTAAAAGG AGAGGGTGAA CATCAAGTGG CAATTTATGA GAGAAAAAGA 
TTAGCAACAA TGATACTGGC AAGAATGTCG TGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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IRA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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