SHE3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SHE3
Entrez Gene ID	852427
Full Name	She3p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178478.3	NP_009688.3	She3p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SHE3	NP_009688.3
Kluyveromyces lactis	KLLA0D09042g	XP_453465.1
Eremothecium gossypii	AGOS_AAR042W	NP_982583.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The cytoplasmic transport factor She3p displaces Loc1p from the ASH1 mRNP, allowing for the maturation of the localizing ASH1 mRNP.
Title: Role of Loc1p in assembly and reorganization of nuclear ASH1 messenger ribonucleoprotein particles in yeast.

Data indicate that the degradation of She3 by Grr1 is required for optimal cell growth.
Title: Identification of She3 as an SCF(Grr1) substrate in budding yeast.

The adapter protein She3p tightly binds to the Myo4p rod, forming a single-headed motor complex.
Title: Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex.

The present study reveals that the current accepted model for ASH1 mRNA localization does not fully account for the function of She3p in ASH1 mRNA localization.
Title: She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae.

continuous transport of mRNA by Myo4p-She3p in yeast is accomplished either by multiple high duty cycle monomers or by molecules that may be dimerized by She2p, the homodimeric downstream binding partner of She3p
Title: She3p binds to the rod of yeast myosin V and prevents it from dimerizing, forming a single-headed motor complex.

The following SHE3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHE3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01834	NM_001178478.3 Latest version!	Saccharomyces cerevisiae S288C She3p (SHE3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHE3 ( NM_001178478.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009688.3 Saccharomyces cerevisiae S288C She3p (SHE3), partial mRNA.

Price & Availability↑

CloneID	OSi01834
Clone ID Related Accession (Same CDS sequence)	NM_001178478.3
Accession Version	NM_001178478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	She3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178478.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGGACC AGGATAATAC CCAGACTTCT TCAAGCAAGT TGGCACCTCA CCATAATATT 
TTTATGGCAA ACTTGGAAAG TTCCCCCACA AAAGATAGAA ATACCTCCAG CCAAAATGCC 
TCATCTTCGA GAGTCATTGA ATCACTGCAT GACCAGATTG ATATGCTGAC AAAAACAAAT 
CTACAATTGA CTACGCAATC TCAAAATTTA TTGAGTAAAT TGGAATTAGC ACAATCCAAA 
GAATCAAAAC TACTGGAAAA TTTAAATCTG CTGAAGAACG AGAACGAAAA TCTCAACTCG 
ATATTTGAAA GAAAGAACAA GAAGCTAAAA GAGCTAGAGA AAGATTATAG CGAATTGAGT 
AACCGTTACA ATGAACAAAA GGAGAAAATG GATCAATTGA GTAAATTGGC GAAAAATTCT 
AGTGCCATTG AACAATCTTG CTCTGAAAAA TTGCAAAATA TGGAAGTTAA CTATAATTCA 
TTATTGGAGT CACAAAATCT TTACAGAGAC CACTATTCTG ATGAAATTTC CAAATTAAAT 
GAAAAAATCG GTTTGTTGGA GTTAGAGTTA AGTAATCAAA ATTTAAATTA TGGTTCAGAT 
ACTAGTTCAA ATTCAGATAT AGAATTGAAT TTAAATAAAT TCAATGACTC TGTGAAAGAT 
TTGAAATCTC TGGAAACAGA AAAGGATTCA AAATTAAGCA AGATAATCAC TCACTCTTTA 
GATGAGTTAA ATCTACAAAG CTGGCTAAAT CTGTACCAGA CGAACGAAAA TCTGATATCA 
ACTTTTGCTG AAAAGATGGA TTTGAAAGAC GTCTTGAAAA GAAACGATGA GAAGATTAGT 
AATAAAGGCG CGGTAGTACA AACCTTGAAG AAGAATGTAC AAACCCAGGT GGAAAGCAAC 
AACGCTGACG CTTTGAGTAG CAATAATGCA CAGGATATGC TTCCTATTAA GATGGTCAAA 
TTAAGAAAGA CGCCGAACAC AAACGATTCA TCCTCCAATG GTAATAGCAG TAACAATAAA 
AGAAGAAGTT TCTATACTGC GTCGCCTTTG CTGTCATCGG GTTCTATTCC AAAATCTGCA 
TCCCCGGTTT TGCCTGGTGT TAAAAGAACT GCCTCAGTAA GAAAACCAAG CTCGAGCAGT 
AGTAAAACAA ATGTAACGCA TAATAACGAT CCAAGTACAT CTCCCACAAT CTCAGTGCCT 
CCCGGTGTTA CAAGAACTGT TTCCTCCACT CATAAGAAAA AGGGAAATAG TATGGTTGTT 
CACGGGGCCC AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009688.3
CDS	1..1278
Translation	MSDQDNTQTSSSKLAPHHNIFMANLESSPTKDRNTSSQNASSSRVIESLHDQIDMLTKTNLQLTTQSQNLLSKLELAQSKESKLLENLNLLKNENENLNSIFERKNKKLKELEKDYSELSNRYNEQKEKMDQLSKLAKNSSAIEQSCSEKLQNMEVNYNSLLESQNLYRDHYSDEISKLNEKIGLLELELSNQNLNYGSDTSSNSDIELNLNKFNDSVKDLKSLETEKDSKLSKIITHSLDELNLQSWLNLYQTNENLISTFAEKMDLKDVLKRNDEKISNKGAVVQTLKKNVQTQVESNNADALSSNNAQDMLPIKMVKLRKTPNTNDSSSNGNSSNNKRRSFYTASPLLSSGSIPKSASPVLPGVKRTASVRKPSSSSSKTNVTHNNDPSTSPTISVPPGVTRTVSSTHKKKGNSMVVHGAQS

Target ORF information:

RefSeq Version	NM_001178478.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C She3p (SHE3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178478.3

ORF Insert Sequence:

ATGTCGGACC AGGATAATAC CCAGACTTCT TCAAGCAAGT TGGCACCTCA CCATAATATT 
TTTATGGCAA ACTTGGAAAG TTCCCCCACA AAAGATAGAA ATACCTCCAG CCAAAATGCC 
TCATCTTCGA GAGTCATTGA ATCACTGCAT GACCAGATTG ATATGCTGAC AAAAACAAAT 
CTACAATTGA CTACGCAATC TCAAAATTTA TTGAGTAAAT TGGAATTAGC ACAATCCAAA 
GAATCAAAAC TACTGGAAAA TTTAAATCTG CTGAAGAACG AGAACGAAAA TCTCAACTCG 
ATATTTGAAA GAAAGAACAA GAAGCTAAAA GAGCTAGAGA AAGATTATAG CGAATTGAGT 
AACCGTTACA ATGAACAAAA GGAGAAAATG GATCAATTGA GTAAATTGGC GAAAAATTCT 
AGTGCCATTG AACAATCTTG CTCTGAAAAA TTGCAAAATA TGGAAGTTAA CTATAATTCA 
TTATTGGAGT CACAAAATCT TTACAGAGAC CACTATTCTG ATGAAATTTC CAAATTAAAT 
GAAAAAATCG GTTTGTTGGA GTTAGAGTTA AGTAATCAAA ATTTAAATTA TGGTTCAGAT 
ACTAGTTCAA ATTCAGATAT AGAATTGAAT TTAAATAAAT TCAATGACTC TGTGAAAGAT 
TTGAAATCTC TGGAAACAGA AAAGGATTCA AAATTAAGCA AGATAATCAC TCACTCTTTA 
GATGAGTTAA ATCTACAAAG CTGGCTAAAT CTGTACCAGA CGAACGAAAA TCTGATATCA 
ACTTTTGCTG AAAAGATGGA TTTGAAAGAC GTCTTGAAAA GAAACGATGA GAAGATTAGT 
AATAAAGGCG CGGTAGTACA AACCTTGAAG AAGAATGTAC AAACCCAGGT GGAAAGCAAC 
AACGCTGACG CTTTGAGTAG CAATAATGCA CAGGATATGC TTCCTATTAA GATGGTCAAA 
TTAAGAAAGA CGCCGAACAC AAACGATTCA TCCTCCAATG GTAATAGCAG TAACAATAAA 
AGAAGAAGTT TCTATACTGC GTCGCCTTTG CTGTCATCGG GTTCTATTCC AAAATCTGCA 
TCCCCGGTTT TGCCTGGTGT TAAAAGAACT GCCTCAGTAA GAAAACCAAG CTCGAGCAGT 
AGTAAAACAA ATGTAACGCA TAATAACGAT CCAAGTACAT CTCCCACAAT CTCAGTGCCT 
CCCGGTGTTA CAAGAACTGT TTCCTCCACT CATAAGAAAA AGGGAAATAG TATGGTTGTT 
CACGGGGCCC AATCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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SHE3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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