VID24 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VID24
Entrez Gene ID	852402
Full Name	glucose-induced degradation complex subunit VID24
Synonyms	GID4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178453.1	NP_009663.1	glucose-induced degradation complex subunit VID24

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	VID24	NP_009663.1
Kluyveromyces lactis	KLLA0B09592g	XP_451956.1
Eremothecium gossypii	AGOS_ADL330W	NP_983766.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gid4 subunit of the ubiquitin ligase GID in the yeast Saccharomyces cerevisiae targeted the gluconeogenic enzymes Fbp1, Icl1, and Mdh2 for degradation. Gid4 recognized the N-terminal proline residue and the ~5-residue-long adjacent sequence motifs.
Title: An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes.

Vid24p and Sec28p are present at actin patches during glucose starvation.
Title: The vacuole import and degradation pathway utilizes early steps of endocytosis and actin polymerization to deliver cargo proteins to the vacuole for degradation.

the yeast GID complex is a novel ubiquitin ligase (E3) involved in the regulation of carbohydrate metabolism, which is regulated by Gid4/Vid24
Title: The yeast GID complex, a novel ubiquitin ligase (E3) involved in the regulation of carbohydrate metabolism.

The following VID24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VID24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01812	NM_001178453.1 Latest version!	Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID24 (VID24), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VID24 ( NM_001178453.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009663.1 Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID24 (VID24), partial mRNA.

Price & Availability↑

CloneID	OSi01812
Clone ID Related Accession (Same CDS sequence)	NM_001178453.1
Accession Version	NM_001178453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	glucose-induced degradation complex subunit VID24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATCAATA ATCCTAAGGT AGACAGTGTA GCGGAGAAAC CCAAAGCTGT GACATCAAAG 
CAGTCGGAGC AAGCGGCTTC GCCAGAACCA ACACCAGCCC CCCCTGTTTC TAGAAATCAG 
TATCCGATCA CGTTCAACTT GACTTCAACC GCACCCTTTC ATCTTCATGA CCGTCATCGC 
TACTTACAAG AGCAAGATCT TTACAAGTGC GCTTCTAGGG ATTCGTTGTC TTCCCTGCAG 
CAACTCGCCC ATACACCTAA TGGGTCGACA AGGAAGAAAT ATATTGTTGA GGACCAATCT 
CCCTATAGTT CGGAAAATCC AGTCATTGTG ACCTCTTCCT ATAACCATAC GGTTTGCACA 
AACTACTTAA GACCAAGAAT GCAGTTTACA GGGTACCAGA TATCAGGATA CAAACGCTAT 
CAGGTAACAG TTAACTTAAA GACTGTAGAT TTGCCAAAGA AAGATTGCAC GTCGCTGTCC 
CCTCATTTAT CTGGATTTTT GTCGATAAGA GGACTCACGA ACCAACACCC GGAAATCAGT 
ACATATTTTG AAGCCTACGC GGTAAACCAC AAGGAATTAG GGTTCTTGTC CTCTAGCTGG 
AAAGATGAAC CTGTTTTAAA CGAATTCAAA GCCACAGACC AAACAGACTT AGAACACTGG 
ATAAATTTCC CCTCCTTTAG ACAGCTTTTC CTGATGAGCC AAAAAAACGG TCTCAACTCA 
ACTGACGACA ATGGCACTAC AAATGCAGCC AAGAAGTTGC CTCCACAGCA GCTTCCTACT 
ACACCAAGCG CAGACGCTGG TAACATATCA AGAATTTTTA GCCAAGAGAA ACAATTTGAC 
AACTACTTGA ACGAACGGTT TATATTTATG AAATGGAAGG AAAAATTTTT GGTACCAGAT 
GCCCTATTAA TGGAAGGTGT AGACGGCGCA TCTTATGATG GGTTTTATTA CATTGTCCAT 
GATCAAGTTA CCGGGAACAT TCAAGGGTTT TACTATCATC AAGATGCTGA AAAGTTCCAA 
CAGCTGGAAT TAGTACCATC TTTGAAAAAT AAAGTCGAGT CCAGTGATTG TTCTTTTGAG 
TTTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009663.1
CDS	1..1089
Translation	MINNPKVDSVAEKPKAVTSKQSEQAASPEPTPAPPVSRNQYPITFNLTSTAPFHLHDRHRYLQEQDLYKCASRDSLSSLQQLAHTPNGSTRKKYIVEDQSPYSSENPVIVTSSYNHTVCTNYLRPRMQFTGYQISGYKRYQVTVNLKTVDLPKKDCTSLSPHLSGFLSIRGLTNQHPEISTYFEAYAVNHKELGFLSSSWKDEPVLNEFKATDQTDLEHWINFPSFRQLFLMSQKNGLNSTDDNGTTNAAKKLPPQQLPTTPSADAGNISRIFSQEKQFDNYLNERFIFMKWKEKFLVPDALLMEGVDGASYDGFYYIVHDQVTGNIQGFYYHQDAEKFQQLELVPSLKNKVESSDCSFEFA

Target ORF information:

RefSeq Version	NM_001178453.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID24 (VID24), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178453.1

ORF Insert Sequence:

ATGATCAATA ATCCTAAGGT AGACAGTGTA GCGGAGAAAC CCAAAGCTGT GACATCAAAG 
CAGTCGGAGC AAGCGGCTTC GCCAGAACCA ACACCAGCCC CCCCTGTTTC TAGAAATCAG 
TATCCGATCA CGTTCAACTT GACTTCAACC GCACCCTTTC ATCTTCATGA CCGTCATCGC 
TACTTACAAG AGCAAGATCT TTACAAGTGC GCTTCTAGGG ATTCGTTGTC TTCCCTGCAG 
CAACTCGCCC ATACACCTAA TGGGTCGACA AGGAAGAAAT ATATTGTTGA GGACCAATCT 
CCCTATAGTT CGGAAAATCC AGTCATTGTG ACCTCTTCCT ATAACCATAC GGTTTGCACA 
AACTACTTAA GACCAAGAAT GCAGTTTACA GGGTACCAGA TATCAGGATA CAAACGCTAT 
CAGGTAACAG TTAACTTAAA GACTGTAGAT TTGCCAAAGA AAGATTGCAC GTCGCTGTCC 
CCTCATTTAT CTGGATTTTT GTCGATAAGA GGACTCACGA ACCAACACCC GGAAATCAGT 
ACATATTTTG AAGCCTACGC GGTAAACCAC AAGGAATTAG GGTTCTTGTC CTCTAGCTGG 
AAAGATGAAC CTGTTTTAAA CGAATTCAAA GCCACAGACC AAACAGACTT AGAACACTGG 
ATAAATTTCC CCTCCTTTAG ACAGCTTTTC CTGATGAGCC AAAAAAACGG TCTCAACTCA 
ACTGACGACA ATGGCACTAC AAATGCAGCC AAGAAGTTGC CTCCACAGCA GCTTCCTACT 
ACACCAAGCG CAGACGCTGG TAACATATCA AGAATTTTTA GCCAAGAGAA ACAATTTGAC 
AACTACTTGA ACGAACGGTT TATATTTATG AAATGGAAGG AAAAATTTTT GGTACCAGAT 
GCCCTATTAA TGGAAGGTGT AGACGGCGCA TCTTATGATG GGTTTTATTA CATTGTCCAT 
GATCAAGTTA CCGGGAACAT TCAAGGGTTT TACTATCATC AAGATGCTGA AAAGTTCCAA 
CAGCTGGAAT TAGTACCATC TTTGAAAAAT AAAGTCGAGT CCAGTGATTG TTCTTTTGAG 
TTTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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VID24 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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