VPS15 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS15
Entrez Gene ID	852394
Full Name	ubiquitin-binding serine/threonine protein kinase VPS15
Synonyms	GRD8,VAC4,VPL19,VPS40,VPT15
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178445.2	NP_009655.2	ubiquitin-binding serine/threonine protein kinase VPS15

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PIK3R4	XP_004939498.1
Danio rerio (zebrafish)	pik3r4	XP_001922676.1
Oryza sativa (rice)	Os02g0796700	NP_001048391.1
Canis lupus familiaris (dog)	PIK3R4	XP_534260.1
Bos taurus (cattle)	PIK3R4	NP_001093784.1
Eremothecium gossypii	AGOS_ADL316C	NP_983780.2
Magnaporthe oryzae (rice blast fungus)	MGG_06100	XP_003711966.1
Neurospora crassa	NCU06626	XP_962368.2
	PIK3R4	XP_002802990.1
Mus musculus (house mouse)	Pik3r4	NP_001074778.1
Rattus norvegicus (Norway rat)	Pik3r4	NP_001102247.1
Saccharomyces cerevisiae (baker's yeast)	VPS15	NP_009655.2
Schizosaccharomyces pombe (fission yeast)	ppk19	NP_595288.1
Xenopus tropicalis (tropical clawed frog)	pik3r4	NP_001107701.1
Pan troglodytes (chimpanzee)	PIK3R4	XP_516746.2
Drosophila melanogaster (fruit fly)	ird1	NP_649868.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000602	XP_310476.5
Arabidopsis thaliana (thale cress)	AT4G29380	NP_194667.1
Homo sapiens (human)	PIK3R4	NP_055417.1
Kluyveromyces lactis	KLLA0B11924g	XP_452060.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutants lacking Vps15 display multiple phenotypes indicating impaired transcription elongation.
Title: Vps factors are required for efficient transcription elongation in budding yeast.

Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidylinositol 3-phosphate.
Title: Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome.

The following VPS15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01806	NM_001178445.2 Latest version!	Saccharomyces cerevisiae S288C ubiquitin-binding serine/threonine protein kinase VPS15 (VPS15), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS15 ( NM_001178445.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009655.2 Saccharomyces cerevisiae S288C ubiquitin-binding serine/threonine protein kinase VPS15 (VPS15), partial mRNA.

Price & Availability↑

CloneID	OSi01806
Clone ID Related Accession (Same CDS sequence)	NM_001178445.2
Accession Version	NM_001178445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ubiquitin-binding serine/threonine protein kinase VPS15
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178445.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGGGCAC AATTATCACT AGTGGTCCAA GCATCACCTT CCATAGCCAT TTTTTCATAT 
ATCGATGTCT TAGAGGAAGT ACACTACGTT TCACAGTTAA ACTCATCAAG ATTCTTAAAA 
ACATGCAAGG CACTGGACCC TAACGGCGAA ATTGTTATCA AAGTGTTTAT TAAACCAAAA 
GACCAATATA GCTTACGACC TTTTCTCCAA CGTATAAGGG CTCAATCGTT TAAGTTGGGA 
CAACTACCGC ACGTTTTAAA CTACAGTAAA TTGATCGAGA CAAATAGAGC CGGCTACATG 
ATACGGCAGC ACTTAAAAAA TAATTTATAT GACAGATTGA GTTTGAGACC TTACTTACAA 
GACATTGAAC TGAAATTCAT TGCTTTCCAG TTGTTAAATG CATTAAAGGA CATTCATAAT 
CTGAATATTG TCCATGGTGA TATAAAGACA GAAAATATCC TAGTAACAAG TTGGAATTGG 
TGTATATTGA CAGATTTTGC TGCATTTATC AAACCCGTAT ATTTGCCTGA AGATAATCCA 
GGTGAATTTT TATTCTACTT CGACACCTCG AAGAGAAGAA CCTGTTATCT AGCCCCGGAG 
AGGTTTAACT CTAAACTTTA CCAAGATGGA AAATCTAACA ATGGTAGGCT AACTAAAGAA 
ATGGACATAT TTAGTCTTGG ATGTGTTATT GCAGAAATAT TTGCTGAAGG AAGACCCATC 
TTCAACTTAT CACAGCTATT CAAATATAAA AGTAATTCAT ATGACGTAAA CAGGGAATTT 
CTCATGGAGG AAATGAATTC TACCGATTTA AGGAACTTGG TTCTAGACAT GATTCAACTA 
GATCCATCCA AAAGACTTTC ATGTGATGAA CTACTGAATA AATATCGTGG CATTTTCTTC 
CCCGATTATT TCTACACTTT CATTTATGAT TATTTCAGAA ATTTGGTTAC TATGACAACA 
AGCACACCGA TATCAGATAA CACTTGCACC AATAGTACCT TGGAAGACAA TGTAAAACTT 
TTAGATGAAA CTACGGAAAA AATATACAGA GATTTTTCCC AAATATGTCA TTGTTTGGAC 
TTTCCTTTAA TAAAAGACGG GGGTGAGATT GGTTCAGACC CCCCAATTTT GGAATCTTAC 
AAAATAGAGA TAGAAATTAG TCGGTTTTTA AACACAAACT TATATTTCCC CCAAAATTAC 
CATTTAGTCT TACAGCAGTT TACCAAAGTA TCCGAAAAGA TAAAATCAGT TAAAGAGGAA 
TGTGCCTTAC TCTTTATCTC TTATTTGTCT CATAGTATAA GAAGTATTGT TTCGACTGCT 
ACGAAACTTA AAAATTTAGA ACTATTAGCA GTATTTGCAC AATTCGTTTC TGATGAAAAT 
AAAATTGATC GAGTCGTACC TTATTTCGTA TGTTGTTTTG AAGATAGTGA CCAGGACGTC 
CAGGCCCTAT CTTTGTTAAC ATTAATCCAG GTACTCACCT CTGTAAGAAA ATTGAATCAA 
TTGAACGAGA ATATATTTGT GGACTACTTA CTTCCGAGAC TGAAAAGATT ACTTATTTCC 
AATAGGCAGA ATACCAATTA TTTAAGGATT GTGTTTGCTA ATTGTTTGAG CGACTTGGCC 
ATTATCATTA ATAGATTTCA AGAATTTACA TTTGCTCAGC ACTGCAATGA TAACTCAATG 
GATAACAACA CGGAAATCAT GGAAAGCAGT ACCAAGTATT CAGCAAAATT GATCCAAAGT 
GTCGAAGATT TAACTGTCTC TTTTTTAACA GATAATGATA CTTATGTAAA GATGGCACTT 
TTGCAAAACA TTCTTCCACT TTGTAAATTT TTTGGTAGGG AAAGAACAAA CGATATTATA 
CTGAGTCATT TAATAACCTA CCTTAATGAT AAGGACCCAG CATTGCGAGT TTCCTTAATT 
CAAACAATAT CCGGAATATC AATTCTTTTG GGTACCGTTA CATTAGAACA GTATATTTTA 
CCATTGTTAA TCCAGACCAT CACTGATTCG GAAGAATTAG TAGTGATCAG TGTTTTACAA 
AGCTTAAAAT CTTTGTTCAA GACTGGGTTG ATTAGGAAAA AATATTATAT TGATATATCA 
AAAACAACAT CTCCCTTGTT GTTGCATCCT AATAATTGGA TAAGACAGTT TACTTTGATG 
ATAATTATAG AAATTATTAA TAAGTTATCA AAAGCCGAAG TGTACTGCAT TCTCTATCCA 
ATAATAAGGC CTTTCTTCGA ATTTGACGTT GAGTTCAACT TCAAATCAAT GATAAGCTGT 
TGCAAGCAAC CAGTGTCAAG ATCGGTTTAC AATCTATTGT GTAGTTGGTC TGTTAGAGCG 
TCAAAATCTT TATTTTGGAA AAAAATCATC ACAAATCATG TAGATTCATT CGGAAATAAT 
AGAATCGAAT TTATAACAAA AAATTACTCA AGTAAAAATT ATGGATTTAA TAAAAGAGAT 
ACGAAATCAA GTTCCTCGCT GAAGGGTATT AAAACATCAT CCACCGTCTA TTCACATGAC 
AACAAGGAAA TTCCCTTAAC TGCTGAAGAC AGAAATTGGA TTGATAAGTT CCACATTATT 
GGGCTAACAG AAAAAGATAT TTGGAAAATT GTGGCTTTGA GGGGTTATGT AATAAGGACA 
GCGAGAGTTA TGGCAGCGAA CCCTGATTTT CCATATAATA ATAGTAATTA CCGTCCATTA 
GTACAGAACT CACCACCTAA CCTAAACCTT ACGAATATTA TGCCAAGAAA CATTTTCTTT 
GATGTAGAGT TTGCTGAAGA GTCGACAAGT GAGGGACAAG ATTCTAACTT AGAGAACCAA 
CAGATATATA AATATGATGA AAGTGAGAAA GATAGCAATA AGCTAAATAT TAACGGTAGC 
AAACAGCTAT CTACCGTCAT GGACATAAAT GGATCACTAA TATTCAAAAA TAAGTCCATT 
GCCACTACTA CTTCTAATTT GAAGAACGTT TTTGTTCAGT TAGAACCAAC GTCCTATCAC 
ATGCATTCTC CAAATCATGG CTTGAAAGAT AATGCAAATG TTAAACCAGA AAGGAAGGTA 
GTCGTCAGCA ACAGCTATGA AGGCGACGTT GAAAGCATAG AAAAATTCCT ATCGACTTTC 
AAAATTTTAC CTCCTCTGAG AGATTATAAG GAGTTTGGGC CTATTCAAGA GATTGTACGG 
AGTCCAAACA TGGGTAATTT GAGGGGCAAG TTGATAGCTA CTTTGATGGA AAACGAACCC 
AATTCTATTA CGTCTTCTGC TGTTTCTCCA GGAGAAACAC CCTATTTAAT AACAGGTTCA 
GATCAAGGTG TAATCAAGAT TTGGAACCTG AAAGAGATTA TCGTGGGCGA GGTTTACTCT 
TCTTCTTTAA CTTATGACTG CTCCTCTACC GTAACTCAGA TAACCATGAT TCCTAACTTT 
GACGCGTTTG CCGTTTCCAG TAAAGATGGA CAAATAATTG TATTAAAGGT TAATCATTAC 
CAACAAGAAA GTGAAGTCAA ATTTTTGAAT TGCGAATGCA TCAGGAAAAT TAACTTGAAG 
AATTTTGGTA AAAATGAATA CGCAGTGAGA ATGAGAGCAT TTGTGAATGA GGAAAAATCT 
CTACTAGTAG CATTGACGAA TTTGTCAAGG GTTATTATAT TTGATATTAG AACCCTGGAG 
AGGTTACAAA TTATAGAGAA TTCTCCAAGG CATGGTGCCG TTTCAAGCAT CTGTATCGAT 
GAAGAGTGTT GTGTCCTAAT TTTGGGGACG ACTAGAGGTA TTATTGATAT ATGGGATATC 
CGTTTCAACG TGCTGATAAG GAGTTGGTCC TTTGGGGACC ACGCACCAAT CACGCATGTG 
GAGGTTTGTC AGTTTTATGG AAAGAATTCT GTAATTGTTG TAGGAGGTAG TTCAAAAACA 
TTTCTAACAA TATGGAATTT TGTTAAGGGG CATTGTCAGT ATGCTTTCAT AAATTCTGAT 
GAACAGCCAT CTATGGAGCA CTTTTTACCA ATTGAGAAAG GCTTAGAAGA ATTAAATTTT 
TGTGGAATCA GGTCTTTAAA CGCACTAAGC ACTATCTCAG TATCTAATGA TAAAATTCTT 
CTTACCGATG AAGCAACAAG TTCCATTGTT ATGTTTAGCC TAAATGAGCT TTCTTCTTCT 
AAAGCAGTAA TAAGTCCTTC AAGATTCAGT GACGTTTTTA TTCCTACACA AGTTACGGCA 
AATCTCACAA TGTTATTGAG AAAAATGAAA CGTACTAGCA CTCATTCAGT AGATGATTCT 
CTATATCATC ATGATATTAT AAATTCTATA TCTACATGTG AAGTTGATGA GACACCTTTG 
CTGGTTGCTT GTGATAACTC AGGGCTTATT GGAATCTTCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009655.2
CDS	1..4365
Translation	MGAQLSLVVQASPSIAIFSYIDVLEEVHYVSQLNSSRFLKTCKALDPNGEIVIKVFIKPKDQYSLRPFLQRIRAQSFKLGQLPHVLNYSKLIETNRAGYMIRQHLKNNLYDRLSLRPYLQDIELKFIAFQLLNALKDIHNLNIVHGDIKTENILVTSWNWCILTDFAAFIKPVYLPEDNPGEFLFYFDTSKRRTCYLAPERFNSKLYQDGKSNNGRLTKEMDIFSLGCVIAEIFAEGRPIFNLSQLFKYKSNSYDVNREFLMEEMNSTDLRNLVLDMIQLDPSKRLSCDELLNKYRGIFFPDYFYTFIYDYFRNLVTMTTSTPISDNTCTNSTLEDNVKLLDETTEKIYRDFSQICHCLDFPLIKDGGEIGSDPPILESYKIEIEISRFLNTNLYFPQNYHLVLQQFTKVSEKIKSVKEECALLFISYLSHSIRSIVSTATKLKNLELLAVFAQFVSDENKIDRVVPYFVCCFEDSDQDVQALSLLTLIQVLTSVRKLNQLNENIFVDYLLPRLKRLLISNRQNTNYLRIVFANCLSDLAIIINRFQEFTFAQHCNDNSMDNNTEIMESSTKYSAKLIQSVEDLTVSFLTDNDTYVKMALLQNILPLCKFFGRERTNDIILSHLITYLNDKDPALRVSLIQTISGISILLGTVTLEQYILPLLIQTITDSEELVVISVLQSLKSLFKTGLIRKKYYIDISKTTSPLLLHPNNWIRQFTLMIIIEIINKLSKAEVYCILYPIIRPFFEFDVEFNFKSMISCCKQPVSRSVYNLLCSWSVRASKSLFWKKIITNHVDSFGNNRIEFITKNYSSKNYGFNKRDTKSSSSLKGIKTSSTVYSHDNKEIPLTAEDRNWIDKFHIIGLTEKDIWKIVALRGYVIRTARVMAANPDFPYNNSNYRPLVQNSPPNLNLTNIMPRNIFFDVEFAEESTSEGQDSNLENQQIYKYDESEKDSNKLNINGSKQLSTVMDINGSLIFKNKSIATTTSNLKNVFVQLEPTSYHMHSPNHGLKDNANVKPERKVVVSNSYEGDVESIEKFLSTFKILPPLRDYKEFGPIQEIVRSPNMGNLRGKLIATLMENEPNSITSSAVSPGETPYLITGSDQGVIKIWNLKEIIVGEVYSSSLTYDCSSTVTQITMIPNFDAFAVSSKDGQIIVLKVNHYQQESEVKFLNCECIRKINLKNFGKNEYAVRMRAFVNEEKSLLVALTNLSRVIIFDIRTLERLQIIENSPRHGAVSSICIDEECCVLILGTTRGIIDIWDIRFNVLIRSWSFGDHAPITHVEVCQFYGKNSVIVVGGSSKTFLTIWNFVKGHCQYAFINSDEQPSMEHFLPIEKGLEELNFCGIRSLNALSTISVSNDKILLTDEATSSIVMFSLNELSSSKAVISPSRFSDVFIPTQVTANLTMLLRKMKRTSTHSVDDSLYHHDIINSISTCEVDETPLLVACDNSGLIGIFQ

Target ORF information:

RefSeq Version	NM_001178445.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ubiquitin-binding serine/threonine protein kinase VPS15 (VPS15), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178445.2

ORF Insert Sequence:

ATGGGGGCAC AATTATCACT AGTGGTCCAA GCATCACCTT CCATAGCCAT TTTTTCATAT 
ATCGATGTCT TAGAGGAAGT ACACTACGTT TCACAGTTAA ACTCATCAAG ATTCTTAAAA 
ACATGCAAGG CACTGGACCC TAACGGCGAA ATTGTTATCA AAGTGTTTAT TAAACCAAAA 
GACCAATATA GCTTACGACC TTTTCTCCAA CGTATAAGGG CTCAATCGTT TAAGTTGGGA 
CAACTACCGC ACGTTTTAAA CTACAGTAAA TTGATCGAGA CAAATAGAGC CGGCTACATG 
ATACGGCAGC ACTTAAAAAA TAATTTATAT GACAGATTGA GTTTGAGACC TTACTTACAA 
GACATTGAAC TGAAATTCAT TGCTTTCCAG TTGTTAAATG CATTAAAGGA CATTCATAAT 
CTGAATATTG TCCATGGTGA TATAAAGACA GAAAATATCC TAGTAACAAG TTGGAATTGG 
TGTATATTGA CAGATTTTGC TGCATTTATC AAACCCGTAT ATTTGCCTGA AGATAATCCA 
GGTGAATTTT TATTCTACTT CGACACCTCG AAGAGAAGAA CCTGTTATCT AGCCCCGGAG 
AGGTTTAACT CTAAACTTTA CCAAGATGGA AAATCTAACA ATGGTAGGCT AACTAAAGAA 
ATGGACATAT TTAGTCTTGG ATGTGTTATT GCAGAAATAT TTGCTGAAGG AAGACCCATC 
TTCAACTTAT CACAGCTATT CAAATATAAA AGTAATTCAT ATGACGTAAA CAGGGAATTT 
CTCATGGAGG AAATGAATTC TACCGATTTA AGGAACTTGG TTCTAGACAT GATTCAACTA 
GATCCATCCA AAAGACTTTC ATGTGATGAA CTACTGAATA AATATCGTGG CATTTTCTTC 
CCCGATTATT TCTACACTTT CATTTATGAT TATTTCAGAA ATTTGGTTAC TATGACAACA 
AGCACACCGA TATCAGATAA CACTTGCACC AATAGTACCT TGGAAGACAA TGTAAAACTT 
TTAGATGAAA CTACGGAAAA AATATACAGA GATTTTTCCC AAATATGTCA TTGTTTGGAC 
TTTCCTTTAA TAAAAGACGG GGGTGAGATT GGTTCAGACC CCCCAATTTT GGAATCTTAC 
AAAATAGAGA TAGAAATTAG TCGGTTTTTA AACACAAACT TATATTTCCC CCAAAATTAC 
CATTTAGTCT TACAGCAGTT TACCAAAGTA TCCGAAAAGA TAAAATCAGT TAAAGAGGAA 
TGTGCCTTAC TCTTTATCTC TTATTTGTCT CATAGTATAA GAAGTATTGT TTCGACTGCT 
ACGAAACTTA AAAATTTAGA ACTATTAGCA GTATTTGCAC AATTCGTTTC TGATGAAAAT 
AAAATTGATC GAGTCGTACC TTATTTCGTA TGTTGTTTTG AAGATAGTGA CCAGGACGTC 
CAGGCCCTAT CTTTGTTAAC ATTAATCCAG GTACTCACCT CTGTAAGAAA ATTGAATCAA 
TTGAACGAGA ATATATTTGT GGACTACTTA CTTCCGAGAC TGAAAAGATT ACTTATTTCC 
AATAGGCAGA ATACCAATTA TTTAAGGATT GTGTTTGCTA ATTGTTTGAG CGACTTGGCC 
ATTATCATTA ATAGATTTCA AGAATTTACA TTTGCTCAGC ACTGCAATGA TAACTCAATG 
GATAACAACA CGGAAATCAT GGAAAGCAGT ACCAAGTATT CAGCAAAATT GATCCAAAGT 
GTCGAAGATT TAACTGTCTC TTTTTTAACA GATAATGATA CTTATGTAAA GATGGCACTT 
TTGCAAAACA TTCTTCCACT TTGTAAATTT TTTGGTAGGG AAAGAACAAA CGATATTATA 
CTGAGTCATT TAATAACCTA CCTTAATGAT AAGGACCCAG CATTGCGAGT TTCCTTAATT 
CAAACAATAT CCGGAATATC AATTCTTTTG GGTACCGTTA CATTAGAACA GTATATTTTA 
CCATTGTTAA TCCAGACCAT CACTGATTCG GAAGAATTAG TAGTGATCAG TGTTTTACAA 
AGCTTAAAAT CTTTGTTCAA GACTGGGTTG ATTAGGAAAA AATATTATAT TGATATATCA 
AAAACAACAT CTCCCTTGTT GTTGCATCCT AATAATTGGA TAAGACAGTT TACTTTGATG 
ATAATTATAG AAATTATTAA TAAGTTATCA AAAGCCGAAG TGTACTGCAT TCTCTATCCA 
ATAATAAGGC CTTTCTTCGA ATTTGACGTT GAGTTCAACT TCAAATCAAT GATAAGCTGT 
TGCAAGCAAC CAGTGTCAAG ATCGGTTTAC AATCTATTGT GTAGTTGGTC TGTTAGAGCG 
TCAAAATCTT TATTTTGGAA AAAAATCATC ACAAATCATG TAGATTCATT CGGAAATAAT 
AGAATCGAAT TTATAACAAA AAATTACTCA AGTAAAAATT ATGGATTTAA TAAAAGAGAT 
ACGAAATCAA GTTCCTCGCT GAAGGGTATT AAAACATCAT CCACCGTCTA TTCACATGAC 
AACAAGGAAA TTCCCTTAAC TGCTGAAGAC AGAAATTGGA TTGATAAGTT CCACATTATT 
GGGCTAACAG AAAAAGATAT TTGGAAAATT GTGGCTTTGA GGGGTTATGT AATAAGGACA 
GCGAGAGTTA TGGCAGCGAA CCCTGATTTT CCATATAATA ATAGTAATTA CCGTCCATTA 
GTACAGAACT CACCACCTAA CCTAAACCTT ACGAATATTA TGCCAAGAAA CATTTTCTTT 
GATGTAGAGT TTGCTGAAGA GTCGACAAGT GAGGGACAAG ATTCTAACTT AGAGAACCAA 
CAGATATATA AATATGATGA AAGTGAGAAA GATAGCAATA AGCTAAATAT TAACGGTAGC 
AAACAGCTAT CTACCGTCAT GGACATAAAT GGATCACTAA TATTCAAAAA TAAGTCCATT 
GCCACTACTA CTTCTAATTT GAAGAACGTT TTTGTTCAGT TAGAACCAAC GTCCTATCAC 
ATGCATTCTC CAAATCATGG CTTGAAAGAT AATGCAAATG TTAAACCAGA AAGGAAGGTA 
GTCGTCAGCA ACAGCTATGA AGGCGACGTT GAAAGCATAG AAAAATTCCT ATCGACTTTC 
AAAATTTTAC CTCCTCTGAG AGATTATAAG GAGTTTGGGC CTATTCAAGA GATTGTACGG 
AGTCCAAACA TGGGTAATTT GAGGGGCAAG TTGATAGCTA CTTTGATGGA AAACGAACCC 
AATTCTATTA CGTCTTCTGC TGTTTCTCCA GGAGAAACAC CCTATTTAAT AACAGGTTCA 
GATCAAGGTG TAATCAAGAT TTGGAACCTG AAAGAGATTA TCGTGGGCGA GGTTTACTCT 
TCTTCTTTAA CTTATGACTG CTCCTCTACC GTAACTCAGA TAACCATGAT TCCTAACTTT 
GACGCGTTTG CCGTTTCCAG TAAAGATGGA CAAATAATTG TATTAAAGGT TAATCATTAC 
CAACAAGAAA GTGAAGTCAA ATTTTTGAAT TGCGAATGCA TCAGGAAAAT TAACTTGAAG 
AATTTTGGTA AAAATGAATA CGCAGTGAGA ATGAGAGCAT TTGTGAATGA GGAAAAATCT 
CTACTAGTAG CATTGACGAA TTTGTCAAGG GTTATTATAT TTGATATTAG AACCCTGGAG 
AGGTTACAAA TTATAGAGAA TTCTCCAAGG CATGGTGCCG TTTCAAGCAT CTGTATCGAT 
GAAGAGTGTT GTGTCCTAAT TTTGGGGACG ACTAGAGGTA TTATTGATAT ATGGGATATC 
CGTTTCAACG TGCTGATAAG GAGTTGGTCC TTTGGGGACC ACGCACCAAT CACGCATGTG 
GAGGTTTGTC AGTTTTATGG AAAGAATTCT GTAATTGTTG TAGGAGGTAG TTCAAAAACA 
TTTCTAACAA TATGGAATTT TGTTAAGGGG CATTGTCAGT ATGCTTTCAT AAATTCTGAT 
GAACAGCCAT CTATGGAGCA CTTTTTACCA ATTGAGAAAG GCTTAGAAGA ATTAAATTTT 
TGTGGAATCA GGTCTTTAAA CGCACTAAGC ACTATCTCAG TATCTAATGA TAAAATTCTT 
CTTACCGATG AAGCAACAAG TTCCATTGTT ATGTTTAGCC TAAATGAGCT TTCTTCTTCT 
AAAGCAGTAA TAAGTCCTTC AAGATTCAGT GACGTTTTTA TTCCTACACA AGTTACGGCA 
AATCTCACAA TGTTATTGAG AAAAATGAAA CGTACTAGCA CTCATTCAGT AGATGATTCT 
CTATATCATC ATGATATTAT AAATTCTATA TCTACATGTG AAGTTGATGA GACACCTTTG 
CTGGTTGCTT GTGATAACTC AGGGCTTATT GGAATCTTCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
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VPS15 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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