PBY1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PBY1
Entrez Gene ID	852391
Full Name	putative tubulin tyrosine ligase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178442.2	NP_009652.2	putative tubulin tyrosine ligase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADL312C	NP_983784.2
Schizosaccharomyces pombe (fission yeast)	SPAC12B10.04	NP_594636.1
Saccharomyces cerevisiae (baker's yeast)	PBY1	NP_009652.2
Kluyveromyces lactis	KLLA0B12012g	XP_452064.1
Magnaporthe oryzae (rice blast fungus)	MGG_02486	XP_003709282.1
Neurospora crassa	NCU04743	XP_960378.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PBY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PBY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01803	NM_001178442.2 Latest version!	Saccharomyces cerevisiae S288C putative tubulin tyrosine ligase (PBY1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PBY1 ( NM_001178442.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009652.2 Saccharomyces cerevisiae S288C putative tubulin tyrosine ligase (PBY1), partial mRNA.

Price & Availability↑

CloneID	OSi01803
Clone ID Related Accession (Same CDS sequence)	NM_001178442.2
Accession Version	NM_001178442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative tubulin tyrosine ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178442.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCGGGTTT TAATAACTAA CGATGATGGT CCCTTAAGTG ATCAGTTTTC ACCATACATT 
AGACCTTTTA TTCAGCACAT TAAAAGAAAT TATCCTGAAT GGAAAATCAC GGTTTGTGTA 
CCTCATGTCC AGAAATCATG GGTGGGTAAG GCTCATCTTG CTGGTAAAAA TTTGACAGCT 
CAATTCATAT ATTCTAAAGT TGACGCTGAA GACAATACTT TTTGGGGCCC ATTTATCCAG 
CCACAAATTA GGTCAGAAAA CTCTAAATTA CCTTATGTTC TCAATGCTGA AATTCCGAAA 
GATACAATTG AGTGGATATT AATCGATGGA ACTCCAGCAT CGTGCGCAAA CATCGGGCTG 
CACCTATTGT CTAATGAACC GTTTGATCTA GTACTGTCGG GTCCAAATGT TGGTAGAAAT 
ACATCTGCTG CTTATATTAC CTCTTCTGGT ACCGTAGGAG GCGCAATGGA ATCTGTTATT 
ACTGGAAATA CAAAGGCCAT TGCTATTTCT TGGGCCTATT TTAATGGATT GAAAAACGTC 
TCCCCACTTT TAATGGAGAA GGCCTCTAAG AGATCTTTAG ATGTTATCAA ACATCTTGTT 
AAGAATTGGG ACCCAAAAAC AGATCTATAT AGTATCAATA TTCCTTTAGT GGAGAGTTTA 
AGTGATGATA CAAAGGTTTA CTATGCACCA ATTTGGGAAA ATAGATGGAT TCCAATATTC 
AACGGCCCTC ACATTAACCT AGAGAATAGC TTTGCTGAGA TCGAAGACGG TAATGAATCA 
TCATCGATTT CCTTTAATTG GGCTCCAAAA TTTGGGGCAC ATAAGGATTC TATTCACTAT 
ATGGACGAAT ACAAGGACAG GACTGTTTTA ACTGATGCTG AAGTTATTGA ATCCGAAATG 
ATCAGTGTCA CTCCCATGAA AGCCACTTTC AAGGGTGTGA ACCACCTTCT TGGAGAGTTG 
AAACTGACTG AGGAAGAAAA TAATTTATCA AAAACAAATA ACCTCATCGT GGTAAGTATA 
GACCCAATGG AATATATATA CAAACCTCTA ACCCACGCCC TGAAAAAATA TCTGCCACAA 
GTGGAGATTG TATCAAACTT GCCAGAATTT GACAATGGAG GGTGTGAAAA AGAAATGAAG 
GTTTTTCATT ACGGTGATTA CGAACAGCTA GATATGGACA AACTAATGGA ACTGCCCAAT 
AATTACTTCA CAAACTCTTA TATATATAGG AAGGCATTGA TAAGAAAACA CTTTCTTTCG 
CATACCATAC AGACTTATAC TGCGAAGAAC CCAGAGTCCA TTTTAAAGAA GGCATATTTG 
GAATCATTCA CTATTGATTT AGATTATGCT GAATTTCTAG ACGACGCATT AGATGAAAAT 
TGGGAATTAC GCCAAGAGTT GGAGAATGAA AGCCAAGATA AATGGTGGAT CGTGAAACCG 
AGTATGAGCG ATAAAGGTCA AGGTATCAGG GTATTTAAGA CTATTGAAGA TTTACAGGCT 
ATTTTCGATT CCTTTGACGA TGAAGACAGC GAAGCAGAAG AGAGTGGAAA TGACGACGAT 
GCTGATGATG TAAATGGCGA ATTCATGGAT AATAACAAGG TTAACATTTC CCAATTGCGC 
CACTTTATTA TACAAGAATA TTTAACCAAT CCGTTACTAT TGGCATCTAT GGATAATAGA 
AAGTTCCACA TAAGATGTTA CGTCGTCTGT AGGGGAGATT TGCAAGTTTT TGTTTATGAT 
AGAATGCTAG CGCTCTTTGC TGCCAAGCCA TTTGTTCCTC TAGATCCATA TGCGTATTCC 
GTAACTGATT TGAAAGATTT GGAATGCCAC TTGACTAACA CATGTCTGCA GAGCAAAAAG 
AAGGATAAAG ATTCTTCTGT TTTGGAGTTT GACTCCATAG AGGAAATTCC AAATGAGAGA 
AAGTCCAATA TTAAGGAGCA AATCCATAGC ATAACGAACG ACGTTTTCTT AGCTGCTGTA 
AATGTAAATA GATTAAACTT CCAACCGTTA CCAAACGCAT TTGAAACATA TGGCGTAGAC 
TTTTTGATTG ATTCGAATTA CGAAGTTAAA TTACTAGAGA TCAACGCTTT CCCAGACTTC 
AAACAAACTG GGAAAGATTT GAAGAACCTC ATTGATGAAT TATTTGATGA CACTGTCAAA 
TACTGTGTTA CCCCCATCTT TAACGAGAAT AGAAATAAGA CAGATGATGA AACAGACCCG 
AACTTCGTAA AAGTCATTGA TTACACTTCA AACGGTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009652.2
CDS	1..2262
Translation	MRVLITNDDGPLSDQFSPYIRPFIQHIKRNYPEWKITVCVPHVQKSWVGKAHLAGKNLTAQFIYSKVDAEDNTFWGPFIQPQIRSENSKLPYVLNAEIPKDTIEWILIDGTPASCANIGLHLLSNEPFDLVLSGPNVGRNTSAAYITSSGTVGGAMESVITGNTKAIAISWAYFNGLKNVSPLLMEKASKRSLDVIKHLVKNWDPKTDLYSINIPLVESLSDDTKVYYAPIWENRWIPIFNGPHINLENSFAEIEDGNESSSISFNWAPKFGAHKDSIHYMDEYKDRTVLTDAEVIESEMISVTPMKATFKGVNHLLGELKLTEEENNLSKTNNLIVVSIDPMEYIYKPLTHALKKYLPQVEIVSNLPEFDNGGCEKEMKVFHYGDYEQLDMDKLMELPNNYFTNSYIYRKALIRKHFLSHTIQTYTAKNPESILKKAYLESFTIDLDYAEFLDDALDENWELRQELENESQDKWWIVKPSMSDKGQGIRVFKTIEDLQAIFDSFDDEDSEAEESGNDDDADDVNGEFMDNNKVNISQLRHFIIQEYLTNPLLLASMDNRKFHIRCYVVCRGDLQVFVYDRMLALFAAKPFVPLDPYAYSVTDLKDLECHLTNTCLQSKKKDKDSSVLEFDSIEEIPNERKSNIKEQIHSITNDVFLAAVNVNRLNFQPLPNAFETYGVDFLIDSNYEVKLLEINAFPDFKQTGKDLKNLIDELFDDTVKYCVTPIFNENRNKTDDETDPNFVKVIDYTSNGW

Target ORF information:

RefSeq Version	NM_001178442.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative tubulin tyrosine ligase (PBY1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178442.2

ORF Insert Sequence:

ATGCGGGTTT TAATAACTAA CGATGATGGT CCCTTAAGTG ATCAGTTTTC ACCATACATT 
AGACCTTTTA TTCAGCACAT TAAAAGAAAT TATCCTGAAT GGAAAATCAC GGTTTGTGTA 
CCTCATGTCC AGAAATCATG GGTGGGTAAG GCTCATCTTG CTGGTAAAAA TTTGACAGCT 
CAATTCATAT ATTCTAAAGT TGACGCTGAA GACAATACTT TTTGGGGCCC ATTTATCCAG 
CCACAAATTA GGTCAGAAAA CTCTAAATTA CCTTATGTTC TCAATGCTGA AATTCCGAAA 
GATACAATTG AGTGGATATT AATCGATGGA ACTCCAGCAT CGTGCGCAAA CATCGGGCTG 
CACCTATTGT CTAATGAACC GTTTGATCTA GTACTGTCGG GTCCAAATGT TGGTAGAAAT 
ACATCTGCTG CTTATATTAC CTCTTCTGGT ACCGTAGGAG GCGCAATGGA ATCTGTTATT 
ACTGGAAATA CAAAGGCCAT TGCTATTTCT TGGGCCTATT TTAATGGATT GAAAAACGTC 
TCCCCACTTT TAATGGAGAA GGCCTCTAAG AGATCTTTAG ATGTTATCAA ACATCTTGTT 
AAGAATTGGG ACCCAAAAAC AGATCTATAT AGTATCAATA TTCCTTTAGT GGAGAGTTTA 
AGTGATGATA CAAAGGTTTA CTATGCACCA ATTTGGGAAA ATAGATGGAT TCCAATATTC 
AACGGCCCTC ACATTAACCT AGAGAATAGC TTTGCTGAGA TCGAAGACGG TAATGAATCA 
TCATCGATTT CCTTTAATTG GGCTCCAAAA TTTGGGGCAC ATAAGGATTC TATTCACTAT 
ATGGACGAAT ACAAGGACAG GACTGTTTTA ACTGATGCTG AAGTTATTGA ATCCGAAATG 
ATCAGTGTCA CTCCCATGAA AGCCACTTTC AAGGGTGTGA ACCACCTTCT TGGAGAGTTG 
AAACTGACTG AGGAAGAAAA TAATTTATCA AAAACAAATA ACCTCATCGT GGTAAGTATA 
GACCCAATGG AATATATATA CAAACCTCTA ACCCACGCCC TGAAAAAATA TCTGCCACAA 
GTGGAGATTG TATCAAACTT GCCAGAATTT GACAATGGAG GGTGTGAAAA AGAAATGAAG 
GTTTTTCATT ACGGTGATTA CGAACAGCTA GATATGGACA AACTAATGGA ACTGCCCAAT 
AATTACTTCA CAAACTCTTA TATATATAGG AAGGCATTGA TAAGAAAACA CTTTCTTTCG 
CATACCATAC AGACTTATAC TGCGAAGAAC CCAGAGTCCA TTTTAAAGAA GGCATATTTG 
GAATCATTCA CTATTGATTT AGATTATGCT GAATTTCTAG ACGACGCATT AGATGAAAAT 
TGGGAATTAC GCCAAGAGTT GGAGAATGAA AGCCAAGATA AATGGTGGAT CGTGAAACCG 
AGTATGAGCG ATAAAGGTCA AGGTATCAGG GTATTTAAGA CTATTGAAGA TTTACAGGCT 
ATTTTCGATT CCTTTGACGA TGAAGACAGC GAAGCAGAAG AGAGTGGAAA TGACGACGAT 
GCTGATGATG TAAATGGCGA ATTCATGGAT AATAACAAGG TTAACATTTC CCAATTGCGC 
CACTTTATTA TACAAGAATA TTTAACCAAT CCGTTACTAT TGGCATCTAT GGATAATAGA 
AAGTTCCACA TAAGATGTTA CGTCGTCTGT AGGGGAGATT TGCAAGTTTT TGTTTATGAT 
AGAATGCTAG CGCTCTTTGC TGCCAAGCCA TTTGTTCCTC TAGATCCATA TGCGTATTCC 
GTAACTGATT TGAAAGATTT GGAATGCCAC TTGACTAACA CATGTCTGCA GAGCAAAAAG 
AAGGATAAAG ATTCTTCTGT TTTGGAGTTT GACTCCATAG AGGAAATTCC AAATGAGAGA 
AAGTCCAATA TTAAGGAGCA AATCCATAGC ATAACGAACG ACGTTTTCTT AGCTGCTGTA 
AATGTAAATA GATTAAACTT CCAACCGTTA CCAAACGCAT TTGAAACATA TGGCGTAGAC 
TTTTTGATTG ATTCGAATTA CGAAGTTAAA TTACTAGAGA TCAACGCTTT CCCAGACTTC 
AAACAAACTG GGAAAGATTT GAAGAACCTC ATTGATGAAT TATTTGATGA CACTGTCAAA 
TACTGTGTTA CCCCCATCTT TAACGAGAAT AGAAATAAGA CAGATGATGA AACAGACCCG 
AACTTCGTAA AAGTCATTGA TTACACTTCA AACGGTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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PBY1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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