PFF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PFF1
Entrez Gene ID	852366
Full Name	Pff1p
Synonyms	YBR075W
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178422.2	NP_009630.3	Pff1p

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06752	XP_003709466.1
Saccharomyces cerevisiae (baker's yeast)	PFF1	NP_009630.3
Kluyveromyces lactis	KLLA0F11748g	XP_455613.1
Eremothecium gossypii	AGOS_AGL209W	NP_986458.1
Schizosaccharomyces pombe (fission yeast)	SPCC1919.12c	NP_588494.1
Neurospora crassa	NCU04133	XP_961289.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01782	NM_001178422.2 Latest version!	Saccharomyces cerevisiae S288C Pff1p (PFF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFF1 ( NM_001178422.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009630.3 Saccharomyces cerevisiae S288C Pff1p (PFF1), partial mRNA.

Price & Availability↑

CloneID	OSi01782
Clone ID Related Accession (Same CDS sequence)	NM_001178422.2
Accession Version	NM_001178422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Pff1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178422.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAATTAA AAAGTGTATT CAGATCAGTT CTAAAGTACC GGAAAACAAA TTTGAGTTTG 
CTTCTATTAA TCACCTATTC GATTATTACG TTACTCTATA TCTTTGATCA TGAACGTTAT 
AAACTCAATC TACCAAAAGA GGATGAGCAC CCTGAATTCA ATGACTTATT AGAAACAGCA 
TGGGGGGACT TACAGATTAT CACGGCCTCT TTTCATCCGT ACACGTCCAA AGAAAATGAT 
AAAGTACATG ATTACTTGCT CAAAAGGGTT CTTGAAATTA CAGGGAATTC TTCGTTTGCA 
AGTGTTTCAG ATGACAAAGA AAGCGAGAGA TCCATATTAT TTCAGCAACA AGATCCTTTC 
AATGAGTCAT CTAGGTTTTC GAGAGTGACA TATTTTGAAT CTTCTAATAT ATTAGTTAAG 
CTTGAGGGTA AAAATCCTGA GGAAGAAGGG TTACTTCTTT CAGCTCATTT CGACTCTGTA 
CCCACAGGAT ATGGAGCTAC CGATGACGGC ATGGGTGTAG TGTCTTTACT AGCGAACCTC 
AAGTACCATA TAAAGCACAG ACCGAACAGG ACTTTAATTT TCAACTTTAA TAATAATGAA 
GAGTTTGGCC TACTAGGTGC ATCAACTTAT TTTGATCACT CATGGTCTAA TTTAACCAAG 
TATGTGATAA ATTTAGAGGG AACTGGTGCT GGTGGTAAAG CTGTACTGTT CCGAACTTCA 
GATACTTCAA CAGCAAGAAT ATACCAACAG TCTGTTAAGG AAAATCCATT TGGTAACTCT 
ATCTACCAGC AAGGTTTTTA TAGCAGATAT GTCAGGAGTG AGACAGACTA CAAGATTTAC 
GAAGAAAATG GCATGAGAGG CTGGGATGTC GCATTCTACA AACCGAGAAA CCTTTATCAT 
ACCATAAAGG ATTCAATACA ATATACCTCC AAGGCATCTT TATGGCATAT GCTACATACT 
TCCTTACAAT TAAGTGCATA TGTGGCTTCT AATTCATTGG ATACAGCTGA CCAAACGCCG 
GCTTGTTATT TTGATTTTAT CGGTTTGAAG TTTTTCGTTA TAAGCGCTAA AACGCTTTTC 
TATTGGAACT GCATATTTCT ATTAGTTTCA CCCGTTGTGG CCATTGGCTT GTATTTAATT 
TCTCGTGATA GAATGACTTG GAAATCTTAT TCATGGCTAT CGTGGACACG CTTTCCTCTT 
TCATTAGCCG CTGGTATTAT TGTCCAAAAG CTTTTCTCTA ACGACATAAT TCGTTCCAAT 
CCTTTGACGT TCTCAAGGAA CTACTTTTGG CCTATCTCGG CTTTTTTCAC TCAAGTAATT 
TTTACCAGTT ATGTTCTGAT TAATTGCTCC AATTTTTTTT TCCCCTGTGC CGATATGAAA 
AGCCTATCAA TTATCGAATT GTTCATTATT TTATGGACCA TTTTACTTTT TACGTCGAAG 
TTATTGTATA GTTCCGACTA TAGGTATACC GGGCTTTATC CTCTCTCGAT TTTCTTTCTT 
CTGAGCACAA TAGCTGCTAT ATTGAGACTA CTTGCACTAG CGTTGGGAAT GAGAACAAGG 
AAGAGGCTTG GTAGAGAATG TAGGGACCAT CACTCCAATT ACAGCTCCCA TTCTCAAATA 
GATATGGAAC GAGACGGTCA GGAAAACTTG GAACAACCCC AAGATCAACT TACATCGTCA 
CAAGACGATC AAGCAAGTAT CCAAGATGAT AATGTGTCAA CTACTTCAGC TGGCCCATCA 
CATAATGTTG ACGAGGATCA TGGTATGGAT TCATCGTCAC AACAACATGA TGAAAGGGTA 
CCATTACTGA AGGGGTCTAA TAGCATGGAG GAAGGATTGA GTACTAGAGA AAATTCTCTA 
AAACTTGAGT ATACCGATTA TGCCTGGATC ATTCAATTTT TGCTAATTGT ACCAATACCA 
TCATTTATTT TGTTTAACAG TGTGGATGTT ATCATGGACG CACTAAACCA TACTGTCCAG 
GAAGGAAGTA AGGCTACATT TGATGTACTC AGGTTCGGAA TGGTGGGCAG TATTTTGATC 
GCGCTACCAA TTTTACCTTT TTTCTATAAG GTCAACTATA TCACAATTTC TTTAACTGCA 
CTGCTTTTCT TGATTTCCGC ATCCAAAACG CTCCTAGTGC ACCCCTTTAC CAATAGTAAC 
CCATTAAAAG TAAGGTTTTC TCAAAATATT GATTTGTCTC AAGGGAACGC TGCCAGTGTA 
CATGTCCTTG GAAGAGAAGG AAATTTTTTA AAACCTATGC TGCAAGATTT ACCAAGTATA 
AAATATAGCT CTACACATAT TAACTGTACT TCCGTAACCA ATGGAATGGA ACTTTGTATG 
TATGATGGAA TGCAACCTAA TTTATTGAGC ACGAATGGAA ATACGAACAT CAGCAGTATG 
GTCAAGGTTC ATGTTTTACA TAATAACCGA AACTCAACAG AGAGATCTCC TTACGAACCA 
ATAGTTGCAG AGTTACTTTT GGAGGTGAAG GAGAACAGGG CTTGCACTTT AACTTTTGAA 
AGTAGACATC AGGCGAAGTC ACCTGTCAGA GAAATCACGG TATATCAAAA GAAAAATTCG 
GCCCCACAGA AAGCTAATAT CACGAAGACG ATCAAGTCTG CTTCTGGCAT AAACGAACTG 
CAATTGCATA AACTTGATTT CGATCAGGAA ACTTACCATA TCGGAGTCCA GTGGTTTCCA 
AAACTTTTGA CGGATGGAAA TGTAGAGGAT GACAAGTTGG GGACAAAAGA CGAACTGTCT 
GTTTCTATTA GCTGTTATTG GGGAGAATAT GATTCGGAAT CGGTTGTAAA TGGTACCGCT 
GTTAGGAAAA TTCCAGCATT TGATGAATTG ATAAATTACG CACCTCTAAG TTTTTCCTTT 
ACCAACGAAC AGAAAGGATT AGTTATTGTC AAGGATGCTA TAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009630.3
CDS	1..2931
Translation	MKLKSVFRSVLKYRKTNLSLLLLITYSIITLLYIFDHERYKLNLPKEDEHPEFNDLLETAWGDLQIITASFHPYTSKENDKVHDYLLKRVLEITGNSSFASVSDDKESERSILFQQQDPFNESSRFSRVTYFESSNILVKLEGKNPEEEGLLLSAHFDSVPTGYGATDDGMGVVSLLANLKYHIKHRPNRTLIFNFNNNEEFGLLGASTYFDHSWSNLTKYVINLEGTGAGGKAVLFRTSDTSTARIYQQSVKENPFGNSIYQQGFYSRYVRSETDYKIYEENGMRGWDVAFYKPRNLYHTIKDSIQYTSKASLWHMLHTSLQLSAYVASNSLDTADQTPACYFDFIGLKFFVISAKTLFYWNCIFLLVSPVVAIGLYLISRDRMTWKSYSWLSWTRFPLSLAAGIIVQKLFSNDIIRSNPLTFSRNYFWPISAFFTQVIFTSYVLINCSNFFFPCADMKSLSIIELFIILWTILLFTSKLLYSSDYRYTGLYPLSIFFLLSTIAAILRLLALALGMRTRKRLGRECRDHHSNYSSHSQIDMERDGQENLEQPQDQLTSSQDDQASIQDDNVSTTSAGPSHNVDEDHGMDSSSQQHDERVPLLKGSNSMEEGLSTRENSLKLEYTDYAWIIQFLLIVPIPSFILFNSVDVIMDALNHTVQEGSKATFDVLRFGMVGSILIALPILPFFYKVNYITISLTALLFLISASKTLLVHPFTNSNPLKVRFSQNIDLSQGNAASVHVLGREGNFLKPMLQDLPSIKYSSTHINCTSVTNGMELCMYDGMQPNLLSTNGNTNISSMVKVHVLHNNRNSTERSPYEPIVAELLLEVKENRACTLTFESRHQAKSPVREITVYQKKNSAPQKANITKTIKSASGINELQLHKLDFDQETYHIGVQWFPKLLTDGNVEDDKLGTKDELSVSISCYWGEYDSESVVNGTAVRKIPAFDELINYAPLSFSFTNEQKGLVIVKDAIIL

Target ORF information:

RefSeq Version	NM_001178422.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Pff1p (PFF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178422.2

ORF Insert Sequence:

ATGAAATTAA AAAGTGTATT CAGATCAGTT CTAAAGTACC GGAAAACAAA TTTGAGTTTG 
CTTCTATTAA TCACCTATTC GATTATTACG TTACTCTATA TCTTTGATCA TGAACGTTAT 
AAACTCAATC TACCAAAAGA GGATGAGCAC CCTGAATTCA ATGACTTATT AGAAACAGCA 
TGGGGGGACT TACAGATTAT CACGGCCTCT TTTCATCCGT ACACGTCCAA AGAAAATGAT 
AAAGTACATG ATTACTTGCT CAAAAGGGTT CTTGAAATTA CAGGGAATTC TTCGTTTGCA 
AGTGTTTCAG ATGACAAAGA AAGCGAGAGA TCCATATTAT TTCAGCAACA AGATCCTTTC 
AATGAGTCAT CTAGGTTTTC GAGAGTGACA TATTTTGAAT CTTCTAATAT ATTAGTTAAG 
CTTGAGGGTA AAAATCCTGA GGAAGAAGGG TTACTTCTTT CAGCTCATTT CGACTCTGTA 
CCCACAGGAT ATGGAGCTAC CGATGACGGC ATGGGTGTAG TGTCTTTACT AGCGAACCTC 
AAGTACCATA TAAAGCACAG ACCGAACAGG ACTTTAATTT TCAACTTTAA TAATAATGAA 
GAGTTTGGCC TACTAGGTGC ATCAACTTAT TTTGATCACT CATGGTCTAA TTTAACCAAG 
TATGTGATAA ATTTAGAGGG AACTGGTGCT GGTGGTAAAG CTGTACTGTT CCGAACTTCA 
GATACTTCAA CAGCAAGAAT ATACCAACAG TCTGTTAAGG AAAATCCATT TGGTAACTCT 
ATCTACCAGC AAGGTTTTTA TAGCAGATAT GTCAGGAGTG AGACAGACTA CAAGATTTAC 
GAAGAAAATG GCATGAGAGG CTGGGATGTC GCATTCTACA AACCGAGAAA CCTTTATCAT 
ACCATAAAGG ATTCAATACA ATATACCTCC AAGGCATCTT TATGGCATAT GCTACATACT 
TCCTTACAAT TAAGTGCATA TGTGGCTTCT AATTCATTGG ATACAGCTGA CCAAACGCCG 
GCTTGTTATT TTGATTTTAT CGGTTTGAAG TTTTTCGTTA TAAGCGCTAA AACGCTTTTC 
TATTGGAACT GCATATTTCT ATTAGTTTCA CCCGTTGTGG CCATTGGCTT GTATTTAATT 
TCTCGTGATA GAATGACTTG GAAATCTTAT TCATGGCTAT CGTGGACACG CTTTCCTCTT 
TCATTAGCCG CTGGTATTAT TGTCCAAAAG CTTTTCTCTA ACGACATAAT TCGTTCCAAT 
CCTTTGACGT TCTCAAGGAA CTACTTTTGG CCTATCTCGG CTTTTTTCAC TCAAGTAATT 
TTTACCAGTT ATGTTCTGAT TAATTGCTCC AATTTTTTTT TCCCCTGTGC CGATATGAAA 
AGCCTATCAA TTATCGAATT GTTCATTATT TTATGGACCA TTTTACTTTT TACGTCGAAG 
TTATTGTATA GTTCCGACTA TAGGTATACC GGGCTTTATC CTCTCTCGAT TTTCTTTCTT 
CTGAGCACAA TAGCTGCTAT ATTGAGACTA CTTGCACTAG CGTTGGGAAT GAGAACAAGG 
AAGAGGCTTG GTAGAGAATG TAGGGACCAT CACTCCAATT ACAGCTCCCA TTCTCAAATA 
GATATGGAAC GAGACGGTCA GGAAAACTTG GAACAACCCC AAGATCAACT TACATCGTCA 
CAAGACGATC AAGCAAGTAT CCAAGATGAT AATGTGTCAA CTACTTCAGC TGGCCCATCA 
CATAATGTTG ACGAGGATCA TGGTATGGAT TCATCGTCAC AACAACATGA TGAAAGGGTA 
CCATTACTGA AGGGGTCTAA TAGCATGGAG GAAGGATTGA GTACTAGAGA AAATTCTCTA 
AAACTTGAGT ATACCGATTA TGCCTGGATC ATTCAATTTT TGCTAATTGT ACCAATACCA 
TCATTTATTT TGTTTAACAG TGTGGATGTT ATCATGGACG CACTAAACCA TACTGTCCAG 
GAAGGAAGTA AGGCTACATT TGATGTACTC AGGTTCGGAA TGGTGGGCAG TATTTTGATC 
GCGCTACCAA TTTTACCTTT TTTCTATAAG GTCAACTATA TCACAATTTC TTTAACTGCA 
CTGCTTTTCT TGATTTCCGC ATCCAAAACG CTCCTAGTGC ACCCCTTTAC CAATAGTAAC 
CCATTAAAAG TAAGGTTTTC TCAAAATATT GATTTGTCTC AAGGGAACGC TGCCAGTGTA 
CATGTCCTTG GAAGAGAAGG AAATTTTTTA AAACCTATGC TGCAAGATTT ACCAAGTATA 
AAATATAGCT CTACACATAT TAACTGTACT TCCGTAACCA ATGGAATGGA ACTTTGTATG 
TATGATGGAA TGCAACCTAA TTTATTGAGC ACGAATGGAA ATACGAACAT CAGCAGTATG 
GTCAAGGTTC ATGTTTTACA TAATAACCGA AACTCAACAG AGAGATCTCC TTACGAACCA 
ATAGTTGCAG AGTTACTTTT GGAGGTGAAG GAGAACAGGG CTTGCACTTT AACTTTTGAA 
AGTAGACATC AGGCGAAGTC ACCTGTCAGA GAAATCACGG TATATCAAAA GAAAAATTCG 
GCCCCACAGA AAGCTAATAT CACGAAGACG ATCAAGTCTG CTTCTGGCAT AAACGAACTG 
CAATTGCATA AACTTGATTT CGATCAGGAA ACTTACCATA TCGGAGTCCA GTGGTTTCCA 
AAACTTTTGA CGGATGGAAA TGTAGAGGAT GACAAGTTGG GGACAAAAGA CGAACTGTCT 
GTTTCTATTA GCTGTTATTG GGGAGAATAT GATTCGGAAT CGGTTGTAAA TGGTACCGCT 
GTTAGGAAAA TTCCAGCATT TGATGAATTG ATAAATTACG CACCTCTAAG TTTTTCCTTT 
ACCAACGAAC AGAAAGGATT AGTTATTGTC AAGGATGCTA TAATTTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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PFF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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