CHS3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CHS3
Entrez Gene ID	852311
Full Name	chitin synthase CHS3
Synonyms	CAL1,CSD2,DIT101,KTI2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178371.1	NP_009579.1	chitin synthase CHS3

Pathways from BioSystems

Homologies

Neurospora crassa	NCU09324	XP_964028.2
Saccharomyces cerevisiae (baker's yeast)	CHS3	NP_009579.1
Kluyveromyces lactis	KLLA0D03498g	XP_453222.1
Magnaporthe oryzae (rice blast fungus)	MGG_09962	XP_003710073.1
Eremothecium gossypii	AGOS_AEL189W	NP_984672.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the mitotic spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation. The findings from our study highlight the importance of timely endocytosis of cytokinetic enzymes at the division site in safeguarding mitotic spindle integrity during mitotic exit.
Title: Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit.

data show that the oligomerization of Chs3 through its N-terminus is essential for proper protein trafficking and chitin synthesis and is therefore monitored intracellularly.
Title: Oligomerization of the chitin synthase Chs3 is monitored at the Golgi and affects its endocytic recycling.

The anchoring of Chs3 to septins through Chs4 prevents the arrival of this protein at endocytic sites.
Title: Neck compartmentalization as the molecular basis for the different endocytic behaviour of Chs3 during budding or hyperpolarized growth in yeast cells.

the essential role of Bni4 is to target Glc7 to the neck and activate it toward substrates necessary for CSIII recruitment and synthesis of chitin at the bud neck
Title: Protein phosphatase type 1 directs chitin synthesis at the bud neck in Saccharomyces cerevisiae.

Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.
Title: Hyperpolarized growth of Saccharomyces cerevisiae cak1P212S and cla4 mutants weakens cell walls and renders cells dependent on chitin synthase 3.

The following CHS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01734	NM_001178371.1 Latest version!	Saccharomyces cerevisiae S288C chitin synthase CHS3 (CHS3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHS3 ( NM_001178371.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009579.1 Saccharomyces cerevisiae S288C chitin synthase CHS3 (CHS3), partial mRNA.

Price & Availability↑

CloneID	OSi01734
Clone ID Related Accession (Same CDS sequence)	NM_001178371.1
Accession Version	NM_001178371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chitin synthase CHS3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACCGGCT TGAATGGAGA TGATCCTGAT GACTACTATC TGAACCTTAA TCAAGATGAA 
GAGTCTCTAC TTAGGTCAAG ACACAGTGTC GGCTCAGGAG CACCTCATAG ACAAGGCTCT 
TTAGTGCGGC CCGAAAGAAG CCGACTGAAC AATCCTGATA ATCCACATTT TTATTATGCG 
CAGAAAACGC AGGAGCAGAT GAATCACCTG GATGTTTTAC CATCAAGTAC CGGTGTAAAC 
CCAAATGCAA CTCGTCGGAG TGGCTCCCTG CGCTCCAAAG GCTCAGTGAG AAGCAAATTT 
AGTGGCCGCG AAACGGATAG CTATCTTTTA CAAGATATGA ATACTACTGA CAAGAAGGCT 
TCCGTTAAAA TAAGTGATGA AGGTGTTGCG GAAGACGAAT TTGATAAAGA TGGTGATGTG 
GACAATTTCG AAGAAAGCTC CACGCAGCCC ATAAATAAGT CTATCAAACC ATTAAGAAAG 
GAAACGAATG ATACATTGTC ATTTTGGCAG ATGTACTGTT ATTTCATTAC GTTTTGGGCA 
CCTGCTCCAA TTCTTGCTTT CTGCGGGATG CCAAAGAAGG AAAGACAAAT GGCGTGGAGA 
GAAAAGGTTG CTTTAATTTC TGTCATCTTG TACATTGGTG CGATTGTGGC TTTCCTGACT 
TTTGGTTTCA CTAAAACCGT TTGTAGTAGT TCGAAACTAC GTTTGAAAAA CAACGAAGTA 
TCAACAGAAT TTGTCGTAAT TAACGGTAAG GCTTATGAAT TGGATACTTC CTCGCGTTCC 
GGTATACAAG ACGTTGAAGT AGATTCAGAC ACCCTTTATG GGCCCTGGTC AGATGCTGGT 
AAAGATGCTT CGTTCTTGTT TCAAAATGTG AATGGTAACT GTCATAACCT TATAACTCCA 
AAGAGTAATT CTTCCATTCC CCATGACGAT GATAATAATT TAGCATGGTA TTTTCCTTGT 
AAGTTAAAGA ATCAAGATGG CTCTTCGAAG CCAAACTTCA CAGTTGAAAA TTACGCAGGA 
TGGAACTGTC ATACGTCTAA AGAAGATAGG GACGCATTTT ACGGTTTAAA GTCGAAAGCT 
GATGTTTACT TCACTTGGGA TGGTATAAAG AACTCTTCTA GAAACTTGAT TGTTTATAAT 
GGCGACGTTT TGGATTTAGA TCTTCTTGAT TGGTTGGAAA AGGATGACGT TGACTATCCC 
GTTGTATTCG ATGACTTGAA GACTTCAAAT TTACAAGGTT ATGATCTTTC GTTGGTTTTG 
TCAAATGGGC ATGAAAGAAA AATTGCGAGA TGTTTGAGCG AAATTATTAA AGTTGGTGAA 
GTAGACTCCA AAACCGTCGG TTGTATTGCC TCTGATGTCG TTTTGTATGT TTCTCTGGTA 
TTTATTCTTT CAGTGGTGAT AATTAAATTC ATAATTGCCT GCTACTTCCG TTGGACTGTA 
GCTAGGAAAC AAGGTGCATA TATCGTGGAC AATAAAACAA TGGATAAACA CACAAACGAT 
ATCGAGGATT GGTCTAATAA TATTCAAACA AAAGCTCCTC TAAAGGAAGT AGATCCTCAT 
TTGAGGCCAA AGAAATACTC AAAAAAGTCG TTGGGACACA AGCGTGCTTC AACCTTTGAC 
TTGCTGAAAA AACACAGCTC CAAAATGTTT CAATTTAACG AATCTGTGAT AGATCTAGAC 
ACCTCCATGA GCAGTTCACT ACAATCTTCT GGTTCATACA GAGGAATGAC AACAATGACC 
ACTCAAAATG CTTGGAAACT CTCGAATGAA AACAAAGCTG TACATTCCCG TAATCCATCT 
ACTTTGTTGC CTACATCCTC GATGTTTTGG AATAAAGCGA CTTCCTCTCC TGTACCAGGA 
TCATCGCTGA TTCAGAGTCT TGATTCGACG ATTATACATC CCGATATCGT TCAACAACCA 
CCACTGGATT TTATGCCATA CGGGTTCCCA TTGATTCATA CTATCTGTTT TGTTACTTGT 
TATTCTGAGG ATGAAGAGGG TTTAAGAACC ACTTTAGACT CTCTTTCTAC CACAGATTAT 
CCAAATTCCC ATAAACTACT GATGGTTGTT TGTGATGGTT TAATTAAGGG CTCGGGCAAC 
GATAAGACTA CTCCAGAGAT AGCGTTAGGA ATGATGGACG ACTTTGTCAC CCCACCTGAT 
GAAGTTAAAC CTTACTCCTA TGTGGCAGTG GCATCAGGCT CTAAAAGACA CAATATGGCC 
AAGATATATG CGGGTTTTTA CAAATATGAC GATTCTACAA TTCCACCAGA AAATCAACAA 
CGTGTCCCAA TCATTACAAT TGTGAAGTGC GGTACTCCTG CAGAGCAGGG GGCCGCCAAA 
CCCGGTAACA GAGGTAAGCG TGATTCTCAA ATTATTCTGA TGTCCTTTTT AGAAAAAATA 
ACATTTGATG AAAGAATGAC TCAATTGGAA TTTCAGCTTT TAAAAAATAT TTGGCAGATT 
ACGGGGCTAA TGGCAGACTT CTACGAAACG GTACTTATGG TTGATGCTGA TACTAAAGTC 
TTTCCCGATG CTTTAACTCA TATGGTCGCT GAAATGGTTA AAGATCCTTT GATTATGGGT 
CTTTGTGGTG AGACCAAGAT CGCTAATAAG GCACAATCTT GGGTAACTGC AATTCAAGTG 
TTTGAGTACT ATATTTCGCA TCATCAGGCT AAAGCTTTTG AATCTGTCTT CGGTTCGGTA 
ACTTGTTTGC CGGGATGTTT CTCAATGTAT CGTATAAAAT CTCCTAAAGG TTCAGATGGT 
TATTGGGTAC CTGTATTGGC AAATCCAGAT ATTGTTGAAA GATATTCGGA TAATGTTACA 
AACACTTTGC ATAAGAAGAA CTTATTATTA CTTGGTGAAG ATAGATTTTT ATCTTCATTA 
ATGTTAAAGA CTTTCCCTAA GAGAAAGCAA GTATTTGTTC CAAAAGCTGC TTGTAAAACT 
ATTGCCCCTG ATAAATTCAA AGTCTTACTT TCCCAGCGTC GAAGATGGAT TAATTCTACG 
GTACATAACC TTTTTGAATT AGTTCTAATC AGAGACTTAT GTGGCACTTT CTGTTTTTCC 
ATGCAATTTG TGATTGGTAT TGAATTGATT GGTACTATGG TACTGCCGTT AGCCATTTGC 
TTTACTATTT ATGTCATTAT TTTTGCCATT GTATCAAAAC CTACACCCGT AATCACTTTA 
GTTTTACTGG CAATTATTCT TGGTCTGCCC GGCTTAATTG TTGTTATAAC TGCTACGAGA 
TGGTCGTACC TATGGTGGAT GTGCGTATAT ATTTGTGCTT TGCCTATTTG GAATTTCGTA 
CTACCTTCAT ATGCGTACTG GAAATTTGAT GACTTCTCAT GGGGTGATAC GAGAACTATT 
GCGGGAGGTA ATAAAAAGGC ACAAGACGAG AATGAAGGTG AATTTGATCA CTCAAAGATT 
AAAATGAGGA CATGGAGGGA ATTTGAAAGG GAAGATATTC TCAATCGGAA GGAGGAAAGT 
GACTCCTTCG TTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009579.1
CDS	1..3498
Translation	MTGLNGDDPDDYYLNLNQDEESLLRSRHSVGSGAPHRQGSLVRPERSRLNNPDNPHFYYAQKTQEQMNHLDVLPSSTGVNPNATRRSGSLRSKGSVRSKFSGRETDSYLLQDMNTTDKKASVKISDEGVAEDEFDKDGDVDNFEESSTQPINKSIKPLRKETNDTLSFWQMYCYFITFWAPAPILAFCGMPKKERQMAWREKVALISVILYIGAIVAFLTFGFTKTVCSSSKLRLKNNEVSTEFVVINGKAYELDTSSRSGIQDVEVDSDTLYGPWSDAGKDASFLFQNVNGNCHNLITPKSNSSIPHDDDNNLAWYFPCKLKNQDGSSKPNFTVENYAGWNCHTSKEDRDAFYGLKSKADVYFTWDGIKNSSRNLIVYNGDVLDLDLLDWLEKDDVDYPVVFDDLKTSNLQGYDLSLVLSNGHERKIARCLSEIIKVGEVDSKTVGCIASDVVLYVSLVFILSVVIIKFIIACYFRWTVARKQGAYIVDNKTMDKHTNDIEDWSNNIQTKAPLKEVDPHLRPKKYSKKSLGHKRASTFDLLKKHSSKMFQFNESVIDLDTSMSSSLQSSGSYRGMTTMTTQNAWKLSNENKAVHSRNPSTLLPTSSMFWNKATSSPVPGSSLIQSLDSTIIHPDIVQQPPLDFMPYGFPLIHTICFVTCYSEDEEGLRTTLDSLSTTDYPNSHKLLMVVCDGLIKGSGNDKTTPEIALGMMDDFVTPPDEVKPYSYVAVASGSKRHNMAKIYAGFYKYDDSTIPPENQQRVPIITIVKCGTPAEQGAAKPGNRGKRDSQIILMSFLEKITFDERMTQLEFQLLKNIWQITGLMADFYETVLMVDADTKVFPDALTHMVAEMVKDPLIMGLCGETKIANKAQSWVTAIQVFEYYISHHQAKAFESVFGSVTCLPGCFSMYRIKSPKGSDGYWVPVLANPDIVERYSDNVTNTLHKKNLLLLGEDRFLSSLMLKTFPKRKQVFVPKAACKTIAPDKFKVLLSQRRRWINSTVHNLFELVLIRDLCGTFCFSMQFVIGIELIGTMVLPLAICFTIYVIIFAIVSKPTPVITLVLLAIILGLPGLIVVITATRWSYLWWMCVYICALPIWNFVLPSYAYWKFDDFSWGDTRTIAGGNKKAQDENEGEFDHSKIKMRTWREFEREDILNRKEESDSFVA

Target ORF information:

RefSeq Version	NM_001178371.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chitin synthase CHS3 (CHS3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178371.1

ORF Insert Sequence:

ATGACCGGCT TGAATGGAGA TGATCCTGAT GACTACTATC TGAACCTTAA TCAAGATGAA 
GAGTCTCTAC TTAGGTCAAG ACACAGTGTC GGCTCAGGAG CACCTCATAG ACAAGGCTCT 
TTAGTGCGGC CCGAAAGAAG CCGACTGAAC AATCCTGATA ATCCACATTT TTATTATGCG 
CAGAAAACGC AGGAGCAGAT GAATCACCTG GATGTTTTAC CATCAAGTAC CGGTGTAAAC 
CCAAATGCAA CTCGTCGGAG TGGCTCCCTG CGCTCCAAAG GCTCAGTGAG AAGCAAATTT 
AGTGGCCGCG AAACGGATAG CTATCTTTTA CAAGATATGA ATACTACTGA CAAGAAGGCT 
TCCGTTAAAA TAAGTGATGA AGGTGTTGCG GAAGACGAAT TTGATAAAGA TGGTGATGTG 
GACAATTTCG AAGAAAGCTC CACGCAGCCC ATAAATAAGT CTATCAAACC ATTAAGAAAG 
GAAACGAATG ATACATTGTC ATTTTGGCAG ATGTACTGTT ATTTCATTAC GTTTTGGGCA 
CCTGCTCCAA TTCTTGCTTT CTGCGGGATG CCAAAGAAGG AAAGACAAAT GGCGTGGAGA 
GAAAAGGTTG CTTTAATTTC TGTCATCTTG TACATTGGTG CGATTGTGGC TTTCCTGACT 
TTTGGTTTCA CTAAAACCGT TTGTAGTAGT TCGAAACTAC GTTTGAAAAA CAACGAAGTA 
TCAACAGAAT TTGTCGTAAT TAACGGTAAG GCTTATGAAT TGGATACTTC CTCGCGTTCC 
GGTATACAAG ACGTTGAAGT AGATTCAGAC ACCCTTTATG GGCCCTGGTC AGATGCTGGT 
AAAGATGCTT CGTTCTTGTT TCAAAATGTG AATGGTAACT GTCATAACCT TATAACTCCA 
AAGAGTAATT CTTCCATTCC CCATGACGAT GATAATAATT TAGCATGGTA TTTTCCTTGT 
AAGTTAAAGA ATCAAGATGG CTCTTCGAAG CCAAACTTCA CAGTTGAAAA TTACGCAGGA 
TGGAACTGTC ATACGTCTAA AGAAGATAGG GACGCATTTT ACGGTTTAAA GTCGAAAGCT 
GATGTTTACT TCACTTGGGA TGGTATAAAG AACTCTTCTA GAAACTTGAT TGTTTATAAT 
GGCGACGTTT TGGATTTAGA TCTTCTTGAT TGGTTGGAAA AGGATGACGT TGACTATCCC 
GTTGTATTCG ATGACTTGAA GACTTCAAAT TTACAAGGTT ATGATCTTTC GTTGGTTTTG 
TCAAATGGGC ATGAAAGAAA AATTGCGAGA TGTTTGAGCG AAATTATTAA AGTTGGTGAA 
GTAGACTCCA AAACCGTCGG TTGTATTGCC TCTGATGTCG TTTTGTATGT TTCTCTGGTA 
TTTATTCTTT CAGTGGTGAT AATTAAATTC ATAATTGCCT GCTACTTCCG TTGGACTGTA 
GCTAGGAAAC AAGGTGCATA TATCGTGGAC AATAAAACAA TGGATAAACA CACAAACGAT 
ATCGAGGATT GGTCTAATAA TATTCAAACA AAAGCTCCTC TAAAGGAAGT AGATCCTCAT 
TTGAGGCCAA AGAAATACTC AAAAAAGTCG TTGGGACACA AGCGTGCTTC AACCTTTGAC 
TTGCTGAAAA AACACAGCTC CAAAATGTTT CAATTTAACG AATCTGTGAT AGATCTAGAC 
ACCTCCATGA GCAGTTCACT ACAATCTTCT GGTTCATACA GAGGAATGAC AACAATGACC 
ACTCAAAATG CTTGGAAACT CTCGAATGAA AACAAAGCTG TACATTCCCG TAATCCATCT 
ACTTTGTTGC CTACATCCTC GATGTTTTGG AATAAAGCGA CTTCCTCTCC TGTACCAGGA 
TCATCGCTGA TTCAGAGTCT TGATTCGACG ATTATACATC CCGATATCGT TCAACAACCA 
CCACTGGATT TTATGCCATA CGGGTTCCCA TTGATTCATA CTATCTGTTT TGTTACTTGT 
TATTCTGAGG ATGAAGAGGG TTTAAGAACC ACTTTAGACT CTCTTTCTAC CACAGATTAT 
CCAAATTCCC ATAAACTACT GATGGTTGTT TGTGATGGTT TAATTAAGGG CTCGGGCAAC 
GATAAGACTA CTCCAGAGAT AGCGTTAGGA ATGATGGACG ACTTTGTCAC CCCACCTGAT 
GAAGTTAAAC CTTACTCCTA TGTGGCAGTG GCATCAGGCT CTAAAAGACA CAATATGGCC 
AAGATATATG CGGGTTTTTA CAAATATGAC GATTCTACAA TTCCACCAGA AAATCAACAA 
CGTGTCCCAA TCATTACAAT TGTGAAGTGC GGTACTCCTG CAGAGCAGGG GGCCGCCAAA 
CCCGGTAACA GAGGTAAGCG TGATTCTCAA ATTATTCTGA TGTCCTTTTT AGAAAAAATA 
ACATTTGATG AAAGAATGAC TCAATTGGAA TTTCAGCTTT TAAAAAATAT TTGGCAGATT 
ACGGGGCTAA TGGCAGACTT CTACGAAACG GTACTTATGG TTGATGCTGA TACTAAAGTC 
TTTCCCGATG CTTTAACTCA TATGGTCGCT GAAATGGTTA AAGATCCTTT GATTATGGGT 
CTTTGTGGTG AGACCAAGAT CGCTAATAAG GCACAATCTT GGGTAACTGC AATTCAAGTG 
TTTGAGTACT ATATTTCGCA TCATCAGGCT AAAGCTTTTG AATCTGTCTT CGGTTCGGTA 
ACTTGTTTGC CGGGATGTTT CTCAATGTAT CGTATAAAAT CTCCTAAAGG TTCAGATGGT 
TATTGGGTAC CTGTATTGGC AAATCCAGAT ATTGTTGAAA GATATTCGGA TAATGTTACA 
AACACTTTGC ATAAGAAGAA CTTATTATTA CTTGGTGAAG ATAGATTTTT ATCTTCATTA 
ATGTTAAAGA CTTTCCCTAA GAGAAAGCAA GTATTTGTTC CAAAAGCTGC TTGTAAAACT 
ATTGCCCCTG ATAAATTCAA AGTCTTACTT TCCCAGCGTC GAAGATGGAT TAATTCTACG 
GTACATAACC TTTTTGAATT AGTTCTAATC AGAGACTTAT GTGGCACTTT CTGTTTTTCC 
ATGCAATTTG TGATTGGTAT TGAATTGATT GGTACTATGG TACTGCCGTT AGCCATTTGC 
TTTACTATTT ATGTCATTAT TTTTGCCATT GTATCAAAAC CTACACCCGT AATCACTTTA 
GTTTTACTGG CAATTATTCT TGGTCTGCCC GGCTTAATTG TTGTTATAAC TGCTACGAGA 
TGGTCGTACC TATGGTGGAT GTGCGTATAT ATTTGTGCTT TGCCTATTTG GAATTTCGTA 
CTACCTTCAT ATGCGTACTG GAAATTTGAT GACTTCTCAT GGGGTGATAC GAGAACTATT 
GCGGGAGGTA ATAAAAAGGC ACAAGACGAG AATGAAGGTG AATTTGATCA CTCAAAGATT 
AAAATGAGGA CATGGAGGGA ATTTGAAAGG GAAGATATTC TCAATCGGAA GGAGGAAAGT 
GACTCCTTCG TTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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CHS3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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