FUR4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FUR4
Entrez Gene ID	852309
Full Name	uracil permease
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178369.1	NP_009577.1	uracil permease

Pathways from BioSystems

Homologies

Neurospora crassa	NCU07334	XP_962492.1
Saccharomyces cerevisiae (baker's yeast)	DAL4	NP_012294.3
Schizosaccharomyces pombe (fission yeast)	fur4	NP_593511.1
Oryza sativa (rice)	Os02g0666700	NP_001047676.1
Saccharomyces cerevisiae (baker's yeast)	FUR4	NP_009577.1
Schizosaccharomyces pombe (fission yeast)	SPAC29B12.14c	NP_594991.2
Magnaporthe oryzae (rice blast fungus)	MGG_08002	XP_003714953.1
Arabidopsis thaliana (thale cress)	AT5G03555	NP_568122.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Conformation-sensing domain regulates Fur4 substrate-dependent degradation.
Title: Quality control and substrate-dependent downregulation of the nutrient transporter Fur4.

During heat stress, sphingolipids signal turnover of the uracil permease, Fur4.
Title: Slm1 and slm2 are novel substrates of the calcineurin phosphatase required for heat stress-induced endocytosis of the yeast uracil permease.

GPI-anchored proteins are required for the recruitment of membrane proteins Tat2p and Fur4p to the plasma membrane via detergent-resistant membranes.
Title: Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins Tat2p and Fur4p.

analysis of long-term stability of the subdomains of Can1p: Fur4p (another H+ symporter) and Sur7p (a protein of unknown function) occupy the Can1p subdomain
Title: Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells.

The following FUR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FUR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01732	NM_001178369.1 Latest version!	Saccharomyces cerevisiae S288C uracil permease (FUR4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FUR4 ( NM_001178369.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009577.1 Saccharomyces cerevisiae S288C uracil permease (FUR4), partial mRNA.

Price & Availability↑

CloneID	OSi01732
Clone ID Related Accession (Same CDS sequence)	NM_001178369.1
Accession Version	NM_001178369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	uracil permease
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCAGACA ATCTATCATT ACATTTAAGC GGCTCTTCAA AAAGATTGAA CTCTCGCCAA 
CTTATGGAAT CTTCCAATGA GACCTTTGCG CCAAATAATG TGGATTTGGA AAAAGAGTAT 
AAGTCATCTC AGAGTAATAT AACTACCGAA GTTTATGAGG CATCGAGCTT TGAAGAAAAA 
GTAAGCTCAG AAAAACCTCA ATACAGCTCA TTCTGGAAGA AAATCTATTA TGAATATGTG 
GTCGTTGACA AATCAATCTT GGGTGTTTCT ATTCTGGATT CATTTATGTA CAACCAGGAC 
TTGAAGCCCG TCGAAAAAGA AAGGCGGGTT TGGTCCTGGT ACAATTATTG TTACTTCTGG 
CTTGCTGAAT GTTTCAATAT CAACACTTGG CAAATTGCAG CTACAGGTCT ACAACTGGGT 
CTAAATTGGT GGCAGTGTTG GATAACAATT TGGATTGGGT ACGGTTTCGT TGGTGCTTTT 
GTTGTTTTGG CCTCTAGAGT TGGATCTGCT TATCATTTGT CATTCCCTAT ATCATCTAGA 
GCATCATTCG GTATTTTCTT CTCTTTATGG CCCGTTATTA ACAGAGTCGT CATGGCCATC 
GTTTGGTATA GTGTCCAAGC TTATATTGCG GCAACTCCCG TATCATTAAT GCTGAAATCT 
ATCTTTGGAA AAGATTTACA AGACAAAATC CCAGATCACT TTGGATCACC GAATGCTACT 
ACTTACGAGT TCATGTGTTT TTTTATCTTT TGGGCTGCCA GTCTTCCATT TTTACTGGTT 
CCACCTCACA AAATTAGACA CCTGTTTACT GTTAAAGCCG TCTTGGTTCC GTTTGCTTCT 
TTTGGTTTCT TAATTTGGGC TATCAGAAGA GCTCACGGGC GTATTGCCTT AGGATCTTTA 
ACCGATGTAC AACCTCATGG CTCTGCCTTT TCGTGGGCTT TTCTAAGATC ACTAATGGGT 
TGTATGGCTA ATTTCTCCAC AATGGTAATC AACGCTCCAG ATTTCTCTAG ATTTTCAAAA 
AACCCTAACT CCGCTTTATG GTCCCAATTA GTGTGCATTC CATTTTTGTT TTCCATCACT 
TGTTTAATTG GAATTCTAGT CACCGCAGCT GGTTATGAAA TATACGGTAT TAATTACTGG 
TCGCCACTCG ATGTGCTAGA AAAATTTTTA CAGACTACTT ATAATAAGGG CACAAGAGCT 
GGTGTTTTCT TAATCTCTTT TGTTTTCGCC GTAGCTCAGT TAGGTACTAA TATTTCTGCA 
AACTCATTAT CGTGTGGAAC TGATATGTCC GCTATTTTCC CCAAGTTTAT CAATATCAAG 
CGTGGTTCAT TATTTTGTGC AGCCATGGCG CTGTGTATTT GTCCATGGAA TTTAATGGCA 
ACATCAAGTA AATTTACAAT GGCTTTGTCC GCATATGCTA TCTTTTTGTC CAGTATTGCC 
GGTGTTGTCT GCTCAGATTA CTTTGTTGTT AGAAGAGGAT ATATCAAGCT AACACACATA 
TATTCCCATC AAAAGGGCTC TTTTTATATG TACGGAAACA GGTTTGGTAT CAATTGGAGA 
GCTTTGGCTG CATATCTATG TGGGGTGGCT CCTTGCTTGC CCGGTTTCAT CGCGGAGGTG 
GGCGCTCCCG CTATAAAGGT TTCTGATGGA GCTATGAAAC TATATTACCT AAGTTACTGG 
GTAGGATATG GCTTGAGTTT TTCTTCTTAT ACTGCTCTGT GTTACTTCTT CCCTGTACCT 
GGGTGCCCTG TTAACAACAT TATAAAGGAC AAGGGCTGGT TCCAAAGATG GGCCAATGTC 
GATGATTTTG AAGAAGAGTG GAAAGACACA ATTGAGAGGG ATGACCTGGT AGATGACAAT 
ATTAGTGTCT ACGAACACGA ACACGAAAAG ACTTTCATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009577.1
CDS	1..1902
Translation	MPDNLSLHLSGSSKRLNSRQLMESSNETFAPNNVDLEKEYKSSQSNITTEVYEASSFEEKVSSEKPQYSSFWKKIYYEYVVVDKSILGVSILDSFMYNQDLKPVEKERRVWSWYNYCYFWLAECFNINTWQIAATGLQLGLNWWQCWITIWIGYGFVGAFVVLASRVGSAYHLSFPISSRASFGIFFSLWPVINRVVMAIVWYSVQAYIAATPVSLMLKSIFGKDLQDKIPDHFGSPNATTYEFMCFFIFWAASLPFLLVPPHKIRHLFTVKAVLVPFASFGFLIWAIRRAHGRIALGSLTDVQPHGSAFSWAFLRSLMGCMANFSTMVINAPDFSRFSKNPNSALWSQLVCIPFLFSITCLIGILVTAAGYEIYGINYWSPLDVLEKFLQTTYNKGTRAGVFLISFVFAVAQLGTNISANSLSCGTDMSAIFPKFINIKRGSLFCAAMALCICPWNLMATSSKFTMALSAYAIFLSSIAGVVCSDYFVVRRGYIKLTHIYSHQKGSFYMYGNRFGINWRALAAYLCGVAPCLPGFIAEVGAPAIKVSDGAMKLYYLSYWVGYGLSFSSYTALCYFFPVPGCPVNNIIKDKGWFQRWANVDDFEEEWKDTIERDDLVDDNISVYEHEHEKTFI

Target ORF information:

RefSeq Version	NM_001178369.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C uracil permease (FUR4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178369.1

ORF Insert Sequence:

ATGCCAGACA ATCTATCATT ACATTTAAGC GGCTCTTCAA AAAGATTGAA CTCTCGCCAA 
CTTATGGAAT CTTCCAATGA GACCTTTGCG CCAAATAATG TGGATTTGGA AAAAGAGTAT 
AAGTCATCTC AGAGTAATAT AACTACCGAA GTTTATGAGG CATCGAGCTT TGAAGAAAAA 
GTAAGCTCAG AAAAACCTCA ATACAGCTCA TTCTGGAAGA AAATCTATTA TGAATATGTG 
GTCGTTGACA AATCAATCTT GGGTGTTTCT ATTCTGGATT CATTTATGTA CAACCAGGAC 
TTGAAGCCCG TCGAAAAAGA AAGGCGGGTT TGGTCCTGGT ACAATTATTG TTACTTCTGG 
CTTGCTGAAT GTTTCAATAT CAACACTTGG CAAATTGCAG CTACAGGTCT ACAACTGGGT 
CTAAATTGGT GGCAGTGTTG GATAACAATT TGGATTGGGT ACGGTTTCGT TGGTGCTTTT 
GTTGTTTTGG CCTCTAGAGT TGGATCTGCT TATCATTTGT CATTCCCTAT ATCATCTAGA 
GCATCATTCG GTATTTTCTT CTCTTTATGG CCCGTTATTA ACAGAGTCGT CATGGCCATC 
GTTTGGTATA GTGTCCAAGC TTATATTGCG GCAACTCCCG TATCATTAAT GCTGAAATCT 
ATCTTTGGAA AAGATTTACA AGACAAAATC CCAGATCACT TTGGATCACC GAATGCTACT 
ACTTACGAGT TCATGTGTTT TTTTATCTTT TGGGCTGCCA GTCTTCCATT TTTACTGGTT 
CCACCTCACA AAATTAGACA CCTGTTTACT GTTAAAGCCG TCTTGGTTCC GTTTGCTTCT 
TTTGGTTTCT TAATTTGGGC TATCAGAAGA GCTCACGGGC GTATTGCCTT AGGATCTTTA 
ACCGATGTAC AACCTCATGG CTCTGCCTTT TCGTGGGCTT TTCTAAGATC ACTAATGGGT 
TGTATGGCTA ATTTCTCCAC AATGGTAATC AACGCTCCAG ATTTCTCTAG ATTTTCAAAA 
AACCCTAACT CCGCTTTATG GTCCCAATTA GTGTGCATTC CATTTTTGTT TTCCATCACT 
TGTTTAATTG GAATTCTAGT CACCGCAGCT GGTTATGAAA TATACGGTAT TAATTACTGG 
TCGCCACTCG ATGTGCTAGA AAAATTTTTA CAGACTACTT ATAATAAGGG CACAAGAGCT 
GGTGTTTTCT TAATCTCTTT TGTTTTCGCC GTAGCTCAGT TAGGTACTAA TATTTCTGCA 
AACTCATTAT CGTGTGGAAC TGATATGTCC GCTATTTTCC CCAAGTTTAT CAATATCAAG 
CGTGGTTCAT TATTTTGTGC AGCCATGGCG CTGTGTATTT GTCCATGGAA TTTAATGGCA 
ACATCAAGTA AATTTACAAT GGCTTTGTCC GCATATGCTA TCTTTTTGTC CAGTATTGCC 
GGTGTTGTCT GCTCAGATTA CTTTGTTGTT AGAAGAGGAT ATATCAAGCT AACACACATA 
TATTCCCATC AAAAGGGCTC TTTTTATATG TACGGAAACA GGTTTGGTAT CAATTGGAGA 
GCTTTGGCTG CATATCTATG TGGGGTGGCT CCTTGCTTGC CCGGTTTCAT CGCGGAGGTG 
GGCGCTCCCG CTATAAAGGT TTCTGATGGA GCTATGAAAC TATATTACCT AAGTTACTGG 
GTAGGATATG GCTTGAGTTT TTCTTCTTAT ACTGCTCTGT GTTACTTCTT CCCTGTACCT 
GGGTGCCCTG TTAACAACAT TATAAAGGAC AAGGGCTGGT TCCAAAGATG GGCCAATGTC 
GATGATTTTG AAGAAGAGTG GAAAGACACA ATTGAGAGGG ATGACCTGGT AGATGACAAT 
ATTAGTGTCT ACGAACACGA ACACGAAAAG ACTTTCATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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FUR4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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