ALK2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ALK2
Entrez Gene ID	852274
Full Name	protein kinase ALK2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178249.1	NP_009544.1	protein kinase ALK2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C11583g	XP_452717.1
Eremothecium gossypii	AGOS_AFR492W	NP_986039.1
Saccharomyces cerevisiae (baker's yeast)	ALK2	NP_009544.1
Saccharomyces cerevisiae (baker's yeast)	ALK1	NP_011494.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We show that deletion of ALK1 and ALK2 haspin paralogs causes the mislocalization of polarisome components.
Title: Yeast haspin kinase regulates polarity cues necessary for mitotic spindle positioning and is required to tolerate mitotic arrest.

Overexpression of ALK2, but not of ALK1, causes a mitotic arrest, which is correlated to the kinase activity of the protein
Title: Alk1 and Alk2 are two new cell cycle-regulated haspin-like proteins in budding yeast.

The following ALK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01700	NM_001178249.1 Latest version!	Saccharomyces cerevisiae S288C protein kinase ALK2 (ALK2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALK2 ( NM_001178249.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009544.1 Saccharomyces cerevisiae S288C protein kinase ALK2 (ALK2), partial mRNA.

Price & Availability↑

CloneID	OSi01700
Clone ID Related Accession (Same CDS sequence)	NM_001178249.1
Accession Version	NM_001178249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase ALK2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATTTTG ATGCGGTAGC AGATCAACAG ATGACTGACA GAAGGTATTT TGCTCTCGAA 
GTAGCAGAAA GCGATGATGC CGACAGCTCA TTAAATTCTT CATCCATGGG AAGCCCCGCA 
GTGGATGTAG GAAGAAAAGT TTACAAAATC ACTTCGCACA AGGGTTCAGC AGAAGATGAG 
AGTCAATCCT TTTTTACTTC TTCAGATTCT CCAACATCCA AAACAAGGCC TGTAGGTAAA 
ACCATCGAAA ACGATGATTA CTATGGTAAA AGATCTTCTA CAGGTTCATC GCTCAAACAA 
CTCTTCAATA AAATTAATAT TAATGATACC GCTCACTCTT CAAACAAAGA AAATGTATCT 
CAGTCGGTGC TATCGGAAAA CAAGCTGCTC TCTCCATCTA AAAGGTTGTC GAAGCAAGGT 
CTTACAAAGG TGACTAACTC CAAGTTTCGT ACACCCTTGA GACCTATTTC AAACCAATCG 
ACTTTATCAA GGGATGAGCC TGTTAAAGAT TTTAGATCAC TTAAGTTTCG GAGCGGTAGT 
GATTTCAAAT GCTGGGGTGA CGAGAAGACA AGTTCTCATG TTCATTCATC CAGTGTGAAC 
TCAGTTAATT CCTTTACTTC TACCACCTCT TCTTCAAAGT GGAAGTTCTG GAAAAATGAT 
AACCTATTGT CGAGGTCGCT ATCTTCCAGA TCTGTGAATG ACCAAGATCC GAACTTTGTT 
CAGCCAAAAC CAACCAATTC GTTACAAAAG AAGTCTTCAA TTTCAAGTTT TCACAATTCT 
ATTTTTGGTG GTGGCAAACA CACAGAGAAG AAGAGGAATT CTGGATTTAT TATGCCCGAT 
CATCAGAGCA CGAAGGAGCT AAACCACAAA CATTCATCAT CGAACCTTTC CTTTAGAAGT 
TTGAAGCATA AAACATCACA TTCTTCACTA AATAAGCTCA AAGTAAGGCG TAAAGGAAAT 
ACACAAGAAC TGAATCATCC GATCAAAAAA ACTTGCCAAA TATCATTGCC TGTTCCAGAC 
CAAGTCTCAA AGGACAAGAT TCAACTGAAA TTGAAAAATT CAACATCATT GGCATCCTTA 
TCTTCAGAAG TCACTCCCAT AAACACTTTG GACTACAATG ATTCAATTTT GCAACAAATA 
TTACAACTTT GTGATGTTAA GTATATATTA CATGATCTAC GTGAAGCTCA GTCATTAGGC 
TTGTTTACGT TGAATACTAG ATCGGTTCAG CTGTCTCATA ACTTTTGGCA AACTTATCAT 
AGCGATATGC AAACTTCACT CATTTGCAAG AAAGTATGTC TAGGGGCTCT AAGTGATTTG 
ACTACTTCAA ACTTGATATC CTTACATGAA TTGAAATCAT TACGGTTAAT ACAAGGAACT 
AGCGGTGTCG CCAATTTACT GCAAGCTTAT GTTGTGCCCT CAAATCAATG TGAAAATGAC 
CAAAACTTGA TACTGTACTT ATTTTTCAAA TACCAGGGAA CTCCTCTATC AAGGTGCTCT 
AACATTGATT ACTCTCAAGC ATTGTCTATT TTCTGGCAGT GCAGCAGTAT TTTATATGTT 
GCTGAATCCA AATTTCAGCT CGAACACAGG AATCTAACTT TGGACCATAT TTTGATAGAC 
TCTAAAGGGA ATGTTACTTT AATTGATATG AAGTGCTGTC GTTTCTTGAA TATAGACAAC 
AATAAGGCTT CCTATACAAG ATTAGACCAT CATTATTTCT TCCAAGGCCG GGGGACTCTT 
CAATTCGAGA TATATGAACT GATGAGAAGC ATGCTGCCTC AGCCAATATC CTGGGCTACA 
TTTGAACCAA GAACAAACCT ATTATGGTTA TATCACCTAA GTAGCAGCCT GCTAAAAATG 
GCCAAAAAAG CCGTAGTCAG CGGCGCTTTG AACCGGGAAG AAAATATCTT AATCGAGTTG 
ACGCACTTAC TCGATCCTGC TCGAAAACAT TCCAAAACAA TTTTCAAAAA GGAACTTGTT 
ATAAGAACTT GCGGTGATTT GTTATCTTTG AAGGGAGAAA TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009544.1
CDS	1..2031
Translation	MNFDAVADQQMTDRRYFALEVAESDDADSSLNSSSMGSPAVDVGRKVYKITSHKGSAEDESQSFFTSSDSPTSKTRPVGKTIENDDYYGKRSSTGSSLKQLFNKININDTAHSSNKENVSQSVLSENKLLSPSKRLSKQGLTKVTNSKFRTPLRPISNQSTLSRDEPVKDFRSLKFRSGSDFKCWGDEKTSSHVHSSSVNSVNSFTSTTSSSKWKFWKNDNLLSRSLSSRSVNDQDPNFVQPKPTNSLQKKSSISSFHNSIFGGGKHTEKKRNSGFIMPDHQSTKELNHKHSSSNLSFRSLKHKTSHSSLNKLKVRRKGNTQELNHPIKKTCQISLPVPDQVSKDKIQLKLKNSTSLASLSSEVTPINTLDYNDSILQQILQLCDVKYILHDLREAQSLGLFTLNTRSVQLSHNFWQTYHSDMQTSLICKKVCLGALSDLTTSNLISLHELKSLRLIQGTSGVANLLQAYVVPSNQCENDQNLILYLFFKYQGTPLSRCSNIDYSQALSIFWQCSSILYVAESKFQLEHRNLTLDHILIDSKGNVTLIDMKCCRFLNIDNNKASYTRLDHHYFFQGRGTLQFEIYELMRSMLPQPISWATFEPRTNLLWLYHLSSSLLKMAKKAVVSGALNREENILIELTHLLDPARKHSKTIFKKELVIRTCGDLLSLKGEIMQ

Target ORF information:

RefSeq Version	NM_001178249.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase ALK2 (ALK2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178249.1

ORF Insert Sequence:

ATGAATTTTG ATGCGGTAGC AGATCAACAG ATGACTGACA GAAGGTATTT TGCTCTCGAA 
GTAGCAGAAA GCGATGATGC CGACAGCTCA TTAAATTCTT CATCCATGGG AAGCCCCGCA 
GTGGATGTAG GAAGAAAAGT TTACAAAATC ACTTCGCACA AGGGTTCAGC AGAAGATGAG 
AGTCAATCCT TTTTTACTTC TTCAGATTCT CCAACATCCA AAACAAGGCC TGTAGGTAAA 
ACCATCGAAA ACGATGATTA CTATGGTAAA AGATCTTCTA CAGGTTCATC GCTCAAACAA 
CTCTTCAATA AAATTAATAT TAATGATACC GCTCACTCTT CAAACAAAGA AAATGTATCT 
CAGTCGGTGC TATCGGAAAA CAAGCTGCTC TCTCCATCTA AAAGGTTGTC GAAGCAAGGT 
CTTACAAAGG TGACTAACTC CAAGTTTCGT ACACCCTTGA GACCTATTTC AAACCAATCG 
ACTTTATCAA GGGATGAGCC TGTTAAAGAT TTTAGATCAC TTAAGTTTCG GAGCGGTAGT 
GATTTCAAAT GCTGGGGTGA CGAGAAGACA AGTTCTCATG TTCATTCATC CAGTGTGAAC 
TCAGTTAATT CCTTTACTTC TACCACCTCT TCTTCAAAGT GGAAGTTCTG GAAAAATGAT 
AACCTATTGT CGAGGTCGCT ATCTTCCAGA TCTGTGAATG ACCAAGATCC GAACTTTGTT 
CAGCCAAAAC CAACCAATTC GTTACAAAAG AAGTCTTCAA TTTCAAGTTT TCACAATTCT 
ATTTTTGGTG GTGGCAAACA CACAGAGAAG AAGAGGAATT CTGGATTTAT TATGCCCGAT 
CATCAGAGCA CGAAGGAGCT AAACCACAAA CATTCATCAT CGAACCTTTC CTTTAGAAGT 
TTGAAGCATA AAACATCACA TTCTTCACTA AATAAGCTCA AAGTAAGGCG TAAAGGAAAT 
ACACAAGAAC TGAATCATCC GATCAAAAAA ACTTGCCAAA TATCATTGCC TGTTCCAGAC 
CAAGTCTCAA AGGACAAGAT TCAACTGAAA TTGAAAAATT CAACATCATT GGCATCCTTA 
TCTTCAGAAG TCACTCCCAT AAACACTTTG GACTACAATG ATTCAATTTT GCAACAAATA 
TTACAACTTT GTGATGTTAA GTATATATTA CATGATCTAC GTGAAGCTCA GTCATTAGGC 
TTGTTTACGT TGAATACTAG ATCGGTTCAG CTGTCTCATA ACTTTTGGCA AACTTATCAT 
AGCGATATGC AAACTTCACT CATTTGCAAG AAAGTATGTC TAGGGGCTCT AAGTGATTTG 
ACTACTTCAA ACTTGATATC CTTACATGAA TTGAAATCAT TACGGTTAAT ACAAGGAACT 
AGCGGTGTCG CCAATTTACT GCAAGCTTAT GTTGTGCCCT CAAATCAATG TGAAAATGAC 
CAAAACTTGA TACTGTACTT ATTTTTCAAA TACCAGGGAA CTCCTCTATC AAGGTGCTCT 
AACATTGATT ACTCTCAAGC ATTGTCTATT TTCTGGCAGT GCAGCAGTAT TTTATATGTT 
GCTGAATCCA AATTTCAGCT CGAACACAGG AATCTAACTT TGGACCATAT TTTGATAGAC 
TCTAAAGGGA ATGTTACTTT AATTGATATG AAGTGCTGTC GTTTCTTGAA TATAGACAAC 
AATAAGGCTT CCTATACAAG ATTAGACCAT CATTATTTCT TCCAAGGCCG GGGGACTCTT 
CAATTCGAGA TATATGAACT GATGAGAAGC ATGCTGCCTC AGCCAATATC CTGGGCTACA 
TTTGAACCAA GAACAAACCT ATTATGGTTA TATCACCTAA GTAGCAGCCT GCTAAAAATG 
GCCAAAAAAG CCGTAGTCAG CGGCGCTTTG AACCGGGAAG AAAATATCTT AATCGAGTTG 
ACGCACTTAC TCGATCCTGC TCGAAAACAT TCCAAAACAA TTTTCAAAAA GGAACTTGTT 
ATAAGAACTT GCGGTGATTT GTTATCTTTG AAGGGAGAAA TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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ALK2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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