SCT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SCT1
Entrez Gene ID	852271
Full Name	bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1
Synonyms	GAT2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178251.1	NP_009542.1	bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SCT1	NP_009542.1
Kluyveromyces lactis	KLLA0B10538g	XP_451998.1
Eremothecium gossypii	AGOS_AFR489W	NP_986036.2
Schizosaccharomyces pombe (fission yeast)	SPBC1718.04	NP_596450.1
Neurospora crassa	NCU05985	XP_958638.1
Magnaporthe oryzae (rice blast fungus)	MGG_01291	XP_003714214.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

novel mechanism for the regulation of fatty acid desaturation that is based on competition between Ole1p and the glycerol-3-phosphate acyltransferase Sct1p/Gat2p for the common substrate C16:0-CoA
Title: The yeast acyltransferase Sct1p regulates fatty acid desaturation by competing with the desaturase Ole1p.

Gat1 and Gat2 localized to both perinuclear and cortical endoplasmic reticula in actively proliferating cells.
Title: Glycerol-3-phosphate acyltransferases gat1p and gat2p are microsomal phosphoproteins with differential contributions to polarized cell growth.

The following SCT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01698	NM_001178251.1 Latest version!	Saccharomyces cerevisiae S288C bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1 (SCT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCT1 ( NM_001178251.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009542.1 Saccharomyces cerevisiae S288C bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1 (SCT1), partial mRNA.

Price & Availability↑

CloneID	OSi01698
Clone ID Related Accession (Same CDS sequence)	NM_001178251.1
Accession Version	NM_001178251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCTGCAC CAAAACTCAC GGAGAAATTT GCCTCTTCCA AGAGCACACA GAAAACTACG 
AATTACAGTT CCATCGAGGC CAAAAGCGTC AAGACGTCGG CTGATCAGGC ATACATCTAC 
CAAGAGCCTA GCGCTACCAA GAAGATACTT TACTCCATCG CCACATGGCT GTTGTACAAC 
ATCTTCCACT GCTTCTTTAG AGAAATCAGA GGCCGGGGCA GTTTCAAGGT ACCGCAACAG 
GGACCGGTGA TCTTTGTTGC GGCTCCGCAT GCTAACCAGT TCGTCGACCC TGTAATCCTT 
ATGGGCGAGG TGAAGAAATC TGTCAACAGA CGTGTGTCCT TCTTGATTGC GGAGAGCTCA 
TTAAAGCAAC CCCCCATAGG GTTTTTGGCT AGTTTCTTCA TGGCCATAGG CGTGGTAAGG 
CCGCAGGATA ATTTGAAACC GGCAGAAGGT ACTATCCGCG TAGATCCAAC AGACTACAAG 
AGAGTTATCG GCCACGACAC GCATTTCTTG ACTGATTGTA TGCCAAAGGG TCTCATCGGG 
TTACCCAAAT CAATGGGATT TGGAGAAATC CAGTCCATAG AAAGTGACAC GAGTTTGACC 
CTAAGAAAAG AGTTCAAAAT GGCCAAACCA GAGATTAAAA CTGCTTTACT CACCGGCACT 
ACTTATAAAT ATGCCGCTAA AGTCGACCAA TCTTGCGTTT ACCATAGAGT TTTTGAGCAT 
TTGGCCCATA ACAACTGCAT TGGGATCTTT CCTGAAGGTG GGTCCCACGA CAGAACAAAC 
TTGTTGCCCC TGAAAGCAGG TGTGGCGATT ATGGCTCTTG GTTGCATGGA TAAGCATCCT 
GACGTCAATG TTAAGATTGT TCCCTGCGGT ATGAATTATT TCCATCCACA TAAGTTCAGG 
TCGAGAGCGG TTGTTGAATT CGGTGACCCC ATTGAAATAC CGAAGGAACT AGTCGCCAAG 
TACCACAACC CGGAAACGAA CAGAGATGCA GTGAAAGAAT TATTAGATAC CATATCGAAG 
GGTTTACAAT CCGTTACCGT TACATGTTCT GATTATGAAA CTTTGATGGT GGTTCAAACG 
ATAAGAAGAC TATATATGAC ACAATTTAGC ACCAAGTTAC CGTTGCCCTT GATTGTGGAA 
ATGAACAGAA GAATGGTCAA AGGTTACGAA TTCTATAGAA ACGATCCTAA AATAGCGGAC 
TTGACCAAAG ATATAATGGC ATATAATGCC GCCTTGAGAC ACTATAATCT TCCTGATCAC 
CTTGTGGAGG AGGCAAAGGT AAATTTCGCA AAAAACCTCG GACTTGTTTT TTTTAGATCC 
ATCGGGCTCT GCATCCTCTT TTCGTTAGCC ATGCCAGGTA TCATTATGTT CTCACCTGTC 
TTCATATTAG CCAAGAGAAT TTCTCAAGAA AAGGCCCGTA CCGCTTTGTC CAAGTCTACA 
GTTAAAATAA AGGCTAACGA TGTCATTGCC ACGTGGAAAA TCTTGATTGG GATGGGATTT 
GCGCCCTTGC TTTACATCTT TTGGTCCGTT TTAATCACTT ATTACCTCAG ACATAAACCA 
TGGAATAAAA TATATGTTTT TTCCGGGTCT TACATCTCGT GTGTTATAGT CACGTATTCC 
GCCTTAATCG TGGGTGATAT TGGTATGGAT GGTTTCAAAT CTTTGAGACC ACTGGTTTTA 
TCTCTTACAT CTCCAAAGGG CTTGCAAAAG CTACAAAAGG ATCGTAGAAA TCTGGCAGAA 
AGAATAATCG AAGTTGTAAA TAACTTTGGA AGCGAATTAT TCCCCGATTT CGATAGTGCC 
GCCCTACGTG AAGAATTCGA CGTCATCGAT GAAGAGGAAG AAGATCGAAA AACCTCAGAA 
TTGAATCGCA GGAAAATGCT AAGAAAACAG AAAATAAAAA GACAAGAAAA AGATTCGTCA 
TCACCTATCA TCAGCCAACG TGACAACCAC GATGCCTATG AACACCATAA CCAAGATTCC 
GATGGCGTCT CATTGGTCAA TAGTGACAAT TCCCTCTCTA ACATTCCATT ATTCTCTTCT 
ACTTTTCATC GTAAGTCAGA GTCTTCCTTA GCTTCGACAT CCGTTGCACC TTCTTCTTCC 
TCCGAATTTG AGGTAGAAAA CGAAATCTTG GAGGAAAAAA ATGGATTAGC AAGTAAAATC 
GCACAGGCCG TCTTAAACAA GAGAATTGGT GAAAATACTG CCAGGGAAGA GGAAGAGGAA 
GAAGAAGAGG AAGAAGAAGA AGAGGAAGAA GAAGAAGAAG GGAAAGAAGG AGATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009542.1
CDS	1..2280
Translation	MPAPKLTEKFASSKSTQKTTNYSSIEAKSVKTSADQAYIYQEPSATKKILYSIATWLLYNIFHCFFREIRGRGSFKVPQQGPVIFVAAPHANQFVDPVILMGEVKKSVNRRVSFLIAESSLKQPPIGFLASFFMAIGVVRPQDNLKPAEGTIRVDPTDYKRVIGHDTHFLTDCMPKGLIGLPKSMGFGEIQSIESDTSLTLRKEFKMAKPEIKTALLTGTTYKYAAKVDQSCVYHRVFEHLAHNNCIGIFPEGGSHDRTNLLPLKAGVAIMALGCMDKHPDVNVKIVPCGMNYFHPHKFRSRAVVEFGDPIEIPKELVAKYHNPETNRDAVKELLDTISKGLQSVTVTCSDYETLMVVQTIRRLYMTQFSTKLPLPLIVEMNRRMVKGYEFYRNDPKIADLTKDIMAYNAALRHYNLPDHLVEEAKVNFAKNLGLVFFRSIGLCILFSLAMPGIIMFSPVFILAKRISQEKARTALSKSTVKIKANDVIATWKILIGMGFAPLLYIFWSVLITYYLRHKPWNKIYVFSGSYISCVIVTYSALIVGDIGMDGFKSLRPLVLSLTSPKGLQKLQKDRRNLAERIIEVVNNFGSELFPDFDSAALREEFDVIDEEEEDRKTSELNRRKMLRKQKIKRQEKDSSSPIISQRDNHDAYEHHNQDSDGVSLVNSDNSLSNIPLFSSTFHRKSESSLASTSVAPSSSSEFEVENEILEEKNGLASKIAQAVLNKRIGENTAREEEEEEEEEEEEEEEEEEGKEGDA

Target ORF information:

RefSeq Version	NM_001178251.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1 (SCT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178251.1

ORF Insert Sequence:

ATGCCTGCAC CAAAACTCAC GGAGAAATTT GCCTCTTCCA AGAGCACACA GAAAACTACG 
AATTACAGTT CCATCGAGGC CAAAAGCGTC AAGACGTCGG CTGATCAGGC ATACATCTAC 
CAAGAGCCTA GCGCTACCAA GAAGATACTT TACTCCATCG CCACATGGCT GTTGTACAAC 
ATCTTCCACT GCTTCTTTAG AGAAATCAGA GGCCGGGGCA GTTTCAAGGT ACCGCAACAG 
GGACCGGTGA TCTTTGTTGC GGCTCCGCAT GCTAACCAGT TCGTCGACCC TGTAATCCTT 
ATGGGCGAGG TGAAGAAATC TGTCAACAGA CGTGTGTCCT TCTTGATTGC GGAGAGCTCA 
TTAAAGCAAC CCCCCATAGG GTTTTTGGCT AGTTTCTTCA TGGCCATAGG CGTGGTAAGG 
CCGCAGGATA ATTTGAAACC GGCAGAAGGT ACTATCCGCG TAGATCCAAC AGACTACAAG 
AGAGTTATCG GCCACGACAC GCATTTCTTG ACTGATTGTA TGCCAAAGGG TCTCATCGGG 
TTACCCAAAT CAATGGGATT TGGAGAAATC CAGTCCATAG AAAGTGACAC GAGTTTGACC 
CTAAGAAAAG AGTTCAAAAT GGCCAAACCA GAGATTAAAA CTGCTTTACT CACCGGCACT 
ACTTATAAAT ATGCCGCTAA AGTCGACCAA TCTTGCGTTT ACCATAGAGT TTTTGAGCAT 
TTGGCCCATA ACAACTGCAT TGGGATCTTT CCTGAAGGTG GGTCCCACGA CAGAACAAAC 
TTGTTGCCCC TGAAAGCAGG TGTGGCGATT ATGGCTCTTG GTTGCATGGA TAAGCATCCT 
GACGTCAATG TTAAGATTGT TCCCTGCGGT ATGAATTATT TCCATCCACA TAAGTTCAGG 
TCGAGAGCGG TTGTTGAATT CGGTGACCCC ATTGAAATAC CGAAGGAACT AGTCGCCAAG 
TACCACAACC CGGAAACGAA CAGAGATGCA GTGAAAGAAT TATTAGATAC CATATCGAAG 
GGTTTACAAT CCGTTACCGT TACATGTTCT GATTATGAAA CTTTGATGGT GGTTCAAACG 
ATAAGAAGAC TATATATGAC ACAATTTAGC ACCAAGTTAC CGTTGCCCTT GATTGTGGAA 
ATGAACAGAA GAATGGTCAA AGGTTACGAA TTCTATAGAA ACGATCCTAA AATAGCGGAC 
TTGACCAAAG ATATAATGGC ATATAATGCC GCCTTGAGAC ACTATAATCT TCCTGATCAC 
CTTGTGGAGG AGGCAAAGGT AAATTTCGCA AAAAACCTCG GACTTGTTTT TTTTAGATCC 
ATCGGGCTCT GCATCCTCTT TTCGTTAGCC ATGCCAGGTA TCATTATGTT CTCACCTGTC 
TTCATATTAG CCAAGAGAAT TTCTCAAGAA AAGGCCCGTA CCGCTTTGTC CAAGTCTACA 
GTTAAAATAA AGGCTAACGA TGTCATTGCC ACGTGGAAAA TCTTGATTGG GATGGGATTT 
GCGCCCTTGC TTTACATCTT TTGGTCCGTT TTAATCACTT ATTACCTCAG ACATAAACCA 
TGGAATAAAA TATATGTTTT TTCCGGGTCT TACATCTCGT GTGTTATAGT CACGTATTCC 
GCCTTAATCG TGGGTGATAT TGGTATGGAT GGTTTCAAAT CTTTGAGACC ACTGGTTTTA 
TCTCTTACAT CTCCAAAGGG CTTGCAAAAG CTACAAAAGG ATCGTAGAAA TCTGGCAGAA 
AGAATAATCG AAGTTGTAAA TAACTTTGGA AGCGAATTAT TCCCCGATTT CGATAGTGCC 
GCCCTACGTG AAGAATTCGA CGTCATCGAT GAAGAGGAAG AAGATCGAAA AACCTCAGAA 
TTGAATCGCA GGAAAATGCT AAGAAAACAG AAAATAAAAA GACAAGAAAA AGATTCGTCA 
TCACCTATCA TCAGCCAACG TGACAACCAC GATGCCTATG AACACCATAA CCAAGATTCC 
GATGGCGTCT CATTGGTCAA TAGTGACAAT TCCCTCTCTA ACATTCCATT ATTCTCTTCT 
ACTTTTCATC GTAAGTCAGA GTCTTCCTTA GCTTCGACAT CCGTTGCACC TTCTTCTTCC 
TCCGAATTTG AGGTAGAAAA CGAAATCTTG GAGGAAAAAA ATGGATTAGC AAGTAAAATC 
GCACAGGCCG TCTTAAACAA GAGAATTGGT GAAAATACTG CCAGGGAAGA GGAAGAGGAA 
GAAGAAGAGG AAGAAGAAGA AGAGGAAGAA GAAGAAGAAG GGAAAGAAGG AGATGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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SCT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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