PTH2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PTH2
Entrez Gene ID	852223
Full Name	aminoacyl-tRNA hydrolase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178297.2	NP_009496.2	aminoacyl-tRNA hydrolase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E20021g	XP_454857.1
Saccharomyces cerevisiae (baker's yeast)	PTH2	NP_009496.2
Schizosaccharomyces pombe (fission yeast)	SPAC19A8.14	NP_593778.1
Eremothecium gossypii	AGOS_ABR210W	NP_983159.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Pth2 negatively regulates the UBL-UBA protein-mediated shuttling pathway in the ubiquitin-proteasome system.
Title: Yeast Pth2 is a UBL domain-binding protein that participates in the ubiquitin-proteasome pathway.

The following PTH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01654	NM_001178297.2 Latest version!	Saccharomyces cerevisiae S288C aminoacyl-tRNA hydrolase (PTH2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTH2 ( NM_001178297.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009496.2 Saccharomyces cerevisiae S288C aminoacyl-tRNA hydrolase (PTH2), partial mRNA.

Price & Availability↑

CloneID	OSi01654
Clone ID Related Accession (Same CDS sequence)	NM_001178297.2
Accession Version	NM_001178297.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	aminoacyl-tRNA hydrolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178297.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAAAGA TGACAGTTTC TTCGAATTAC ACCATAGCTT TATGGGCTAC TTTCACCGCA 
ATCTCCTTTG CCGTGGGTTA CCAGTTGGGT ACGTCGAACG CATCATCAAC TAAGAAATCC 
TCAGCTACTT TACTGCGTTC TAAAGAAATG AAGGAAGGAA AGCTACACAA TGACACTGAT 
GAGGAGGAAA GTGAAAGCGA AGATGAGAGC GATGAGGACG AAGATATTGA GTCCACTTCA 
TTGAATGATA TACCTGGAGA AGTTAGGATG GCATTGGTGA TTCGTCAAGA TCTTGGCATG 
ACAAAGGGCA AAATAGCGGC ACAATGCTGT CACGCAGCAT TGTCATGTTT TAGACATATT 
GCTACAAACC CCGCGCGTGC TTCATACAAC CCAATTATGA CACAAAGATG GCTAAATGCT 
GGACAGGCTA AAATAACGTT GAAATGTCCA GATAAGTTTA CAATGGACGA GTTGTATGCG 
AAGGCTATAT CACTTGGGGT GAATGCAGCA GTTATTCATG ATGCTGGTAG AACACAGATT 
GCTGCGGGAA GTGCCACCGT ATTGGGTTTA GGACCTGCTC CAAAAGCAGT ATTGGATCAA 
ATAACAGGTG ATTTGAAATT GTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009496.2
CDS	1..627
Translation	MEKMTVSSNYTIALWATFTAISFAVGYQLGTSNASSTKKSSATLLRSKEMKEGKLHNDTDEEESESEDESDEDEDIESTSLNDIPGEVRMALVIRQDLGMTKGKIAAQCCHAALSCFRHIATNPARASYNPIMTQRWLNAGQAKITLKCPDKFTMDELYAKAISLGVNAAVIHDAGRTQIAAGSATVLGLGPAPKAVLDQITGDLKLY

Target ORF information:

RefSeq Version	NM_001178297.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C aminoacyl-tRNA hydrolase (PTH2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178297.2

ORF Insert Sequence:

ATGGAAAAGA TGACAGTTTC TTCGAATTAC ACCATAGCTT TATGGGCTAC TTTCACCGCA 
ATCTCCTTTG CCGTGGGTTA CCAGTTGGGT ACGTCGAACG CATCATCAAC TAAGAAATCC 
TCAGCTACTT TACTGCGTTC TAAAGAAATG AAGGAAGGAA AGCTACACAA TGACACTGAT 
GAGGAGGAAA GTGAAAGCGA AGATGAGAGC GATGAGGACG AAGATATTGA GTCCACTTCA 
TTGAATGATA TACCTGGAGA AGTTAGGATG GCATTGGTGA TTCGTCAAGA TCTTGGCATG 
ACAAAGGGCA AAATAGCGGC ACAATGCTGT CACGCAGCAT TGTCATGTTT TAGACATATT 
GCTACAAACC CCGCGCGTGC TTCATACAAC CCAATTATGA CACAAAGATG GCTAAATGCT 
GGACAGGCTA AAATAACGTT GAAATGTCCA GATAAGTTTA CAATGGACGA GTTGTATGCG 
AAGGCTATAT CACTTGGGGT GAATGCAGCA GTTATTCATG ATGCTGGTAG AACACAGATT 
GCTGCGGGAA GTGCCACCGT ATTGGGTTTA GGACCTGCTC CAAAAGCAGT ATTGGATCAA 
ATAACAGGTG ATTTGAAATT GTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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PTH2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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