ORF » Species Summary » Saccharomyces cerevisiae S288C » ILS1 cDNA ORF clone

ILS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following ILS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ILS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01638 NM_001178316.1
Latest version!		 Saccharomyces cerevisiae S288C isoleucine--tRNA ligase ILS1 (ILS1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ILS1 ( NM_001178316.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009477.1 Saccharomyces cerevisiae S288C isoleucine--tRNA ligase ILS1 (ILS1), partial mRNA.
    Price & Availability↑
    CloneID OSi01638
    Clone ID Related Accession (Same CDS sequence) NM_001178316.1
    Accession Version NM_001178316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3219bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product isoleucine--tRNA ligase ILS1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGAGA GTAACGCACA CTTCTCATTT CCAAAGGAGG AAGAAAAAGT TCTATCTCTT 
    TGGGATGAAA TAGATGCCTT TCATACTTCA TTAGAATTAA CAAAAGACAA ACCGGAGTTT 
    TCCTTCTTCG ATGGGCCTCC ATTTGCCACC GGTACTCCTC ATTACGGTCA TATTCTTGCT 
    TCCACTATTA AGGATATTGT TCCAAGATAC GCTACCATGA CAGGCCACCA CGTGGAAAGA 
    AGATTCGGTT GGGATACACA CGGTGTTCCA ATTGAACATA TCATTGACAA GAAATTAGGT 
    ATCACGGGTA AAGATGACGT CTTCAAGTAT GGTCTTGAAA ACTACAACAA TGAGTGTAGA 
    TCCATTGTTA TGACTTATGC CAGTGATTGG AGAAAAACTA TTGGTCGTTT GGGTCGTTGG 
    ATTGATTTCG ACAACGATTA CAAGACTATG TATCCATCCT TTATGGAGTC CACATGGTGG 
    GCTTTCAAGC AACTTCATGA AAAAGGTCAA GTTTACCGTG GATTCAAAGT TATGCCTTAT 
    TCTACTGGCC TAACCACTCC CTTGAGTAAC TTTGAAGCTC AGCAAAACTA TAAAGATGTT 
    AACGACCCAG CTGTGACCAT TGGTTTCAAT GTTATTGGCC AGGAAAAAAC TCAATTGGTC 
    GCATGGACTA CGACTCCATG GACTTTACCT TCCAATTTGT CGTTATGTGT TAACGCTGAT 
    TTCGAATATG TAAAGATTTA CGACGAAACC AGAGATCGTT ATTTCATCTT ATTAGAATCT 
    TTGATTAAAA CCTTGTATAA GAAGCCTAAG AATGAAAAAT ATAAGATTGT GGAAAAAATT 
    AAAGGTTCTG ATTTGGTTGG TTTGAAGTAT GAACCATTGT TCCCATATTT CGCTGAACAG 
    TTCCATGAAA CAGCTTTTAG AGTTATTTCA GATGATTATG TTACTAGTGA TTCCGGTACT 
    GGTATTGTTC ATAACGCTCC CGCTTTCGGT GAAGAGGATA ATGCTGCTTG TTTGAAGAAC 
    GGCGTCATAT CCGAAGATTC AGTGTTACCT AACGCCATTG ATGACCTTGG TAGATTCACG 
    AAAGACGTTC CTGATTTTGA GGGTGTTTAT GTCAAGGACG CTGATAAGTT GATTATTAAG 
    TACTTAACTA ATACTGGAAA TTTGTTATTG GCATCCCAAA TTCGCCATTC CTATCCATTC 
    TGTTGGAGAT CCGATACCCC ATTGTTATAC CGTTCTGTTC CAGCTTGGTT CGTTCGTGTT 
    AAAAACATTG TCCCTCAAAT GTTGGATTCT GTGATGAAAT CTCACTGGGT TCCTAACACC 
    ATCAAGGAAA AGAGGTTCGC CAACTGGATC GCCAATGCCC GTGACTGGAA CGTTTCCAGA 
    AATAGATATT GGGGTACTCC AATTCCTTTA TGGGTTTCAG ACGATTTTGA GGAGGTCGTC 
    TGTGTTGGTT CTATCAAAGA ATTGGAAGAA TTGACCGGTG TGCGTAACAT CACTGACTTG 
    CATCGTGATG TCATTGACAA ATTGACAATT CCATCCAAGC AAGGTAAGGG TGACTTAAAG 
    AGAATTGAAG AAGTTTTTGA TTGTTGGTTT GAATCTGGTT CTATGCCTTA TGCTTCTCAA 
    CATTATCCAT TTGAAAACAC AGAAAAATTT GACGAAAGAG TTCCAGCTAA TTTCATCTCT 
    GAAGGTTTGG ATCAAACAAG AGGTTGGTTC TACACGTTAG CTGTCTTAGG TACCCATCTA 
    TTTGGCTCTG TTCCATACAA GAACGTCATC GTCTCTGGTA TTGTCTTAGC TGCCGATGGT 
    AGAAAGATGT CTAAATCCTT GAAAAATTAC CCTGATCCAT CCATTGTTCT GAACAAATAT 
    GGTGCGGATG CATTAAGATT GTACTTGATA AACTCACCTG TTTTAAAAGC TGAAAGTTTG 
    AAATTCAAAG AAGAGGGTGT TAAGGAGGTT GTTTCAAAGG TCTTACTACC ATGGTGGAAC 
    TCCTTTAAAT TTTTGGACGG TCAAATTGCC TTGTTGAAAA AGATGTCTAA CATCGACTTC 
    CAATATGATG ATTCTGTGAA AAGTGATAAT GTCATGGACA GATGGATTTT AGCTTCTATG 
    CAATCTCTAG TACAATTCAT TCACGAAGAA ATGGGTCAGT ACAAATTATA CACTGTCGTT 
    CCAAAACTTT TGAATTTCAT TGATGAATTG ACAAACTGGT ATATTAGATT TAATCGTCGT 
    CGTTTGAAGG GTGAAAACGG TGTTGAGGAC TGTTTGAAAG CATTAAATTC TTTATTCGAT 
    GCCTTATTCA CATTTGTCCG TGCCATGGCT CCATTCACCC CATTTTTGTC AGAAAGCATT 
    TATTTGAGAC TGAAGGAATA CATCCCAGAA GCTGTCTTAG CAAAATACGG TAAGGACGGA 
    AGATCAGTCC ATTTCTTATC TTACCCAGTG GTCAAGAAAG AATACTTTGA CGAGGCCATC 
    GAAACTGCAG TTTCTAGAAT GCAATCCGTT ATCGATTTGG GTAGAAACAT TCGTGAAAAG 
    AAAACTATTT CCTTAAAAAC TCCATTGAAG ACTTTGGTCA TCCTTCACAG TGATGAATCA 
    TACTTGAAGG ATGTAGAAGC CTTGAAAAAC TACATTATCG AGGAATTAAA TGTTCGTGAT 
    GTTGTTATCA CCTCCGATGA GGCAAAATAT GGTGTTGAGT ATAAAGCAGT TGCTGATTGG 
    CCTGTATTGG GTAAGAAGTT GAAAAAAGAC GCAAAGAAGG TTAAAGATGC CCTACCATCA 
    GTCACTTCTG AGCAAGTACG GGAATATTTG GAAAGCGGAA AGTTGGAGGT TGCCGGTATT 
    GAACTGGTTA AAGGAGATTT GAATGCTATT AGAGGCTTGC CAGAATCTGC TGTCCAAGCT 
    GGACAAGAAA CCAGAACCGA CCAAGACGTG TTAATCATCA TGGATACAAA TATTTACTCT 
    GAACTAAAGA GTGAAGGTCT CGCAAGAGAG CTGGTCAACA GAATCCAAAA ATTGAGAAAG 
    AAGTGTGGTT TGGAAGCCAC CGACGATGTT TTAGTGGAGT ACGAATTAGT TAAAGATACT 
    ATCGACTTTG AAGCCATTGT CAAAGAACAT TTTGATATGT TAAGCAAGAC CTGTAGATCC 
    GACATTGCCA AATATGACGG CTCAAAGACA GACCCAATTG GTGATGAAGA ACAATCTATT 
    AATGACACCA TTTTCAAATT AAAAGTGTTC AAATTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009477.1
    CDS1..3219
    Translation

    Target ORF information:

    RefSeq Version NM_001178316.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C isoleucine--tRNA ligase ILS1 (ILS1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGAGA GTAACGCACA CTTCTCATTT CCAAAGGAGG AAGAAAAAGT TCTATCTCTT 
    TGGGATGAAA TAGATGCCTT TCATACTTCA TTAGAATTAA CAAAAGACAA ACCGGAGTTT 
    TCCTTCTTCG ATGGGCCTCC ATTTGCCACC GGTACTCCTC ATTACGGTCA TATTCTTGCT 
    TCCACTATTA AGGATATTGT TCCAAGATAC GCTACCATGA CAGGCCACCA CGTGGAAAGA 
    AGATTCGGTT GGGATACACA CGGTGTTCCA ATTGAACATA TCATTGACAA GAAATTAGGT 
    ATCACGGGTA AAGATGACGT CTTCAAGTAT GGTCTTGAAA ACTACAACAA TGAGTGTAGA 
    TCCATTGTTA TGACTTATGC CAGTGATTGG AGAAAAACTA TTGGTCGTTT GGGTCGTTGG 
    ATTGATTTCG ACAACGATTA CAAGACTATG TATCCATCCT TTATGGAGTC CACATGGTGG 
    GCTTTCAAGC AACTTCATGA AAAAGGTCAA GTTTACCGTG GATTCAAAGT TATGCCTTAT 
    TCTACTGGCC TAACCACTCC CTTGAGTAAC TTTGAAGCTC AGCAAAACTA TAAAGATGTT 
    AACGACCCAG CTGTGACCAT TGGTTTCAAT GTTATTGGCC AGGAAAAAAC TCAATTGGTC 
    GCATGGACTA CGACTCCATG GACTTTACCT TCCAATTTGT CGTTATGTGT TAACGCTGAT 
    TTCGAATATG TAAAGATTTA CGACGAAACC AGAGATCGTT ATTTCATCTT ATTAGAATCT 
    TTGATTAAAA CCTTGTATAA GAAGCCTAAG AATGAAAAAT ATAAGATTGT GGAAAAAATT 
    AAAGGTTCTG ATTTGGTTGG TTTGAAGTAT GAACCATTGT TCCCATATTT CGCTGAACAG 
    TTCCATGAAA CAGCTTTTAG AGTTATTTCA GATGATTATG TTACTAGTGA TTCCGGTACT 
    GGTATTGTTC ATAACGCTCC CGCTTTCGGT GAAGAGGATA ATGCTGCTTG TTTGAAGAAC 
    GGCGTCATAT CCGAAGATTC AGTGTTACCT AACGCCATTG ATGACCTTGG TAGATTCACG 
    AAAGACGTTC CTGATTTTGA GGGTGTTTAT GTCAAGGACG CTGATAAGTT GATTATTAAG 
    TACTTAACTA ATACTGGAAA TTTGTTATTG GCATCCCAAA TTCGCCATTC CTATCCATTC 
    TGTTGGAGAT CCGATACCCC ATTGTTATAC CGTTCTGTTC CAGCTTGGTT CGTTCGTGTT 
    AAAAACATTG TCCCTCAAAT GTTGGATTCT GTGATGAAAT CTCACTGGGT TCCTAACACC 
    ATCAAGGAAA AGAGGTTCGC CAACTGGATC GCCAATGCCC GTGACTGGAA CGTTTCCAGA 
    AATAGATATT GGGGTACTCC AATTCCTTTA TGGGTTTCAG ACGATTTTGA GGAGGTCGTC 
    TGTGTTGGTT CTATCAAAGA ATTGGAAGAA TTGACCGGTG TGCGTAACAT CACTGACTTG 
    CATCGTGATG TCATTGACAA ATTGACAATT CCATCCAAGC AAGGTAAGGG TGACTTAAAG 
    AGAATTGAAG AAGTTTTTGA TTGTTGGTTT GAATCTGGTT CTATGCCTTA TGCTTCTCAA 
    CATTATCCAT TTGAAAACAC AGAAAAATTT GACGAAAGAG TTCCAGCTAA TTTCATCTCT 
    GAAGGTTTGG ATCAAACAAG AGGTTGGTTC TACACGTTAG CTGTCTTAGG TACCCATCTA 
    TTTGGCTCTG TTCCATACAA GAACGTCATC GTCTCTGGTA TTGTCTTAGC TGCCGATGGT 
    AGAAAGATGT CTAAATCCTT GAAAAATTAC CCTGATCCAT CCATTGTTCT GAACAAATAT 
    GGTGCGGATG CATTAAGATT GTACTTGATA AACTCACCTG TTTTAAAAGC TGAAAGTTTG 
    AAATTCAAAG AAGAGGGTGT TAAGGAGGTT GTTTCAAAGG TCTTACTACC ATGGTGGAAC 
    TCCTTTAAAT TTTTGGACGG TCAAATTGCC TTGTTGAAAA AGATGTCTAA CATCGACTTC 
    CAATATGATG ATTCTGTGAA AAGTGATAAT GTCATGGACA GATGGATTTT AGCTTCTATG 
    CAATCTCTAG TACAATTCAT TCACGAAGAA ATGGGTCAGT ACAAATTATA CACTGTCGTT 
    CCAAAACTTT TGAATTTCAT TGATGAATTG ACAAACTGGT ATATTAGATT TAATCGTCGT 
    CGTTTGAAGG GTGAAAACGG TGTTGAGGAC TGTTTGAAAG CATTAAATTC TTTATTCGAT 
    GCCTTATTCA CATTTGTCCG TGCCATGGCT CCATTCACCC CATTTTTGTC AGAAAGCATT 
    TATTTGAGAC TGAAGGAATA CATCCCAGAA GCTGTCTTAG CAAAATACGG TAAGGACGGA 
    AGATCAGTCC ATTTCTTATC TTACCCAGTG GTCAAGAAAG AATACTTTGA CGAGGCCATC 
    GAAACTGCAG TTTCTAGAAT GCAATCCGTT ATCGATTTGG GTAGAAACAT TCGTGAAAAG 
    AAAACTATTT CCTTAAAAAC TCCATTGAAG ACTTTGGTCA TCCTTCACAG TGATGAATCA 
    TACTTGAAGG ATGTAGAAGC CTTGAAAAAC TACATTATCG AGGAATTAAA TGTTCGTGAT 
    GTTGTTATCA CCTCCGATGA GGCAAAATAT GGTGTTGAGT ATAAAGCAGT TGCTGATTGG 
    CCTGTATTGG GTAAGAAGTT GAAAAAAGAC GCAAAGAAGG TTAAAGATGC CCTACCATCA 
    GTCACTTCTG AGCAAGTACG GGAATATTTG GAAAGCGGAA AGTTGGAGGT TGCCGGTATT 
    GAACTGGTTA AAGGAGATTT GAATGCTATT AGAGGCTTGC CAGAATCTGC TGTCCAAGCT 
    GGACAAGAAA CCAGAACCGA CCAAGACGTG TTAATCATCA TGGATACAAA TATTTACTCT 
    GAACTAAAGA GTGAAGGTCT CGCAAGAGAG CTGGTCAACA GAATCCAAAA ATTGAGAAAG 
    AAGTGTGGTT TGGAAGCCAC CGACGATGTT TTAGTGGAGT ACGAATTAGT TAAAGATACT 
    ATCGACTTTG AAGCCATTGT CAAAGAACAT TTTGATATGT TAAGCAAGAC CTGTAGATCC 
    GACATTGCCA AATATGACGG CTCAAAGACA GACCCAATTG GTGATGAAGA ACAATCTATT 
    AATGACACCA TTTTCAAATT AAAAGTGTTC AAATTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome II.
    The EMBO journal13(24)5795-809(1994 Dec)
    Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K


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