LCD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	LCD1
Entrez Gene ID	852110
Full Name	Lcd1p
Synonyms	DDC2,PIE1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180807.3	NP_010787.3	Lcd1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C01892g	XP_452280.1
Eremothecium gossypii	AGOS_AFR687W	NP_986235.1
Saccharomyces cerevisiae (baker's yeast)	LCD1	NP_010787.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage.
Title: Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage.

Physical interaction between Pph3 and Mec1, mediated by cofactors Psy2 and Ddc2, is important for regulating the checkpoint response in yeast.
Title: Yeast PP4 interacts with ATR homolog Ddc2-Mec1 and regulates checkpoint signaling.

Our results suggest that Ddc2 plays a critical role in Mec1 activation as well as Mec1 localization at sites of DNA damage
Title: Ddc2 mediates Mec1 activation through a Ddc1- or Dpb11-independent mechanism.

Results point to an evolutionary conserved role for Ddc2/ATRIP in monitoring meiotic chromosome metabolism.
Title: The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates.

Results show that DNA damage response at eroded telomeres is characterized by the recruitment of Cdc13, replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus.
Title: The DNA damage response at eroded telomeres and tethering to the nuclear pore complex.

The following LCD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LCD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01566	NM_001180807.3 Latest version!	Saccharomyces cerevisiae S288C Lcd1p (LCD1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LCD1 ( NM_001180807.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010787.3 Saccharomyces cerevisiae S288C Lcd1p (LCD1), partial mRNA.

Price & Availability↑

CloneID	OSi01566
Clone ID Related Accession (Same CDS sequence)	NM_001180807.3
Accession Version	NM_001180807.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Lcd1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180807.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGACGAG AAACGGTGGG TGAATTTTCT TCAGATGACG ATGATGATAT TCTTTTGGAA 
TTAGGCACCA GGCCTCCAAG GTTTACTCAA ATACCGCCAT CATCAGCAGC ATTACAAACA 
CAAATTCCCA CTACTTTGGA GGTTACAACG ACCACATTAA ACAATAAACA GAGTAAAAAT 
GATAACCAAC TGGTTAACCA ACTGAATAAA GCTCAAGGTG AAGCAAGCAT GCTTCGTGAT 
AAAATAAACT TTTTGAACAT TGAAAGGGAA AAGGAAAAGA ATATTCAAGC CGTCAAAGTT 
AATGAATTGC AAGTCAAGCA TCTTCAAGAG TTGGCTAAAT TAAAACAAGA ATTACAGAAA 
CTGGAAGATG AGAAGAAGTT CTTACAGATG GAAGCGAGAG GAAAATCGAA AAGGGAAGTT 
ATTACGAATG TAAAACCACC GTCAACAACA TTATCAACAA ACACAAACAC TATAACGCCA 
GATTCGTCCT CAGTTGCAAT CGAAGCAAAA CCTCAATCAC CACAATCAAA AAAACGTAAG 
ATAAGTGATA ATTTACTGAA AAAAAATATG GTTCCCCTAA ACCCAAATAG GATTATTCCC 
GATGAAACGA GTTTATTTCT AGAGTCAATA TTACTTCATC AAATAATAGG CGCTGACCTG 
AGCACAATAG AAATATTAAA TAGATTGAAG CTTGACTACA TCACAGAGTT TAAATTTAAG 
AATTTCGTCA TTGCTAAAGG AGCCCCCATA GGGAAGTCCA TAGTTTCTCT ACTTTTGCGA 
TGTAAAAAGA CGTTGACCCT CGACAGGTTC ATAGATACTT TGCTAGAGGA TATAGCTGTT 
TTGATCAAGG AAATATCAGT TCATCCAAAT GAATCGAAAT TAGCTGTCCC ATTTCTCGTT 
GCATTGATGT ATCAGATTGT ACAATTTCGT CCTAGTGCCA CTCATAATTT AGCACTGAAG 
GATTGTTTTC TTTTTATTTG CGATTTAATA AGAATCTATC ATCATGTATT GAAAGTACCG 
ATACATGAAT CAAATATGAA TTTGCATGTA GAACCTCAGA TTTTCCAATA TGAACTGATA 
GACTATTTGA TAATTTCGTA TTCCTTTGAT CTCCTAGAAG GTATATTAAG GGTACTGCAG 
TCGCATCCTA AGCAAACTTA TATGGAATTT TTTGATGAAA ATATTCTAAA ATCATTTGAA 
TTTGTCTACA AACTAGCACT AACCATTTCA TACAAGCCAA TGGTAAATGT AATATTTAGT 
GCAGTCGAGG TCGTTAATAT TATCACTAGT ATAATATTAA ATATGGACAA TTCCTCAGAT 
CTGAAATCCT TGATAAGCGG TAGTTGGTGG AGAGATTGTA TTACAAGATT ATACGCTCTT 
TTGGAAAAGG AAATTAAGAG TGGCGACGTA TATAATGAAA ATGTGGATAC TACAACTCTT 
CATATGTCGA AGTACCATGA CTTTTTTGGG TTAATCCGAA ATATAGGTGA TAATGAATTG 
GGAGGATTGA TATCAAAGCT GATTTATACT GACCGATTGC AAAGTGTCCC AAGGGTAATT 
TCTAAAGAGG ATATTGGGAT GGATAGCGAC AAATTCACTG CCCCTATAAT AGGTTACAAG 
ATGGAAAAAT GGCTTTTGAA GTTAAAAGAT GAAGTTTTAA ATATTTTTGA AAATTTATTA 
ATGATCTATG GAGACGATGC GACCATAGTA AATGGAGAAA TGCTCATCCA CTCCTCTAAA 
TTCTTATCCA GGGAGCAAGC GTTAATGATA GAAAGGTACG TGGGACAAGA CTCCCCGAAC 
TTGGACCTCA GATGCCATCT TATTGAACAT ACTTTAACCA TAATATATAG GCTATGGAAA 
GACCATTTCA AACAATTGCG TGAAGAACAA ATCAAGCAGG TAGAAAGCCA ATTGATTATG 
TCACTATGGA GGTTTCTCGT ATGCCAAACC GAAACTGTGA CAGCAAACGA AAGAGAAATG 
AGAGATCATC GACACCTTGT AGATAGTTTG CATGATCTGA CGATAAAGGA TCAAGCCTCT 
TATTACGAAG ATGCCTTTGA GGATTTACCA GAATATATCG AAGAAGAATT GAAGATGCAA 
TTGAATAAAA GAACGGGGAG AATAATGCAA GTAAAGTACG ATGAGAAATT TCAAGAAATG 
GCAAGAACTA TTCTTGAGTC AAAATCATTC GATCTAACCA CACTAGAGGA GGCCGATTCA 
TTATATATCT CAATGGGACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010787.3
CDS	1..2244
Translation	MRRETVGEFSSDDDDDILLELGTRPPRFTQIPPSSAALQTQIPTTLEVTTTTLNNKQSKNDNQLVNQLNKAQGEASMLRDKINFLNIEREKEKNIQAVKVNELQVKHLQELAKLKQELQKLEDEKKFLQMEARGKSKREVITNVKPPSTTLSTNTNTITPDSSSVAIEAKPQSPQSKKRKISDNLLKKNMVPLNPNRIIPDETSLFLESILLHQIIGADLSTIEILNRLKLDYITEFKFKNFVIAKGAPIGKSIVSLLLRCKKTLTLDRFIDTLLEDIAVLIKEISVHPNESKLAVPFLVALMYQIVQFRPSATHNLALKDCFLFICDLIRIYHHVLKVPIHESNMNLHVEPQIFQYELIDYLIISYSFDLLEGILRVLQSHPKQTYMEFFDENILKSFEFVYKLALTISYKPMVNVIFSAVEVVNIITSIILNMDNSSDLKSLISGSWWRDCITRLYALLEKEIKSGDVYNENVDTTTLHMSKYHDFFGLIRNIGDNELGGLISKLIYTDRLQSVPRVISKEDIGMDSDKFTAPIIGYKMEKWLLKLKDEVLNIFENLLMIYGDDATIVNGEMLIHSSKFLSREQALMIERYVGQDSPNLDLRCHLIEHTLTIIYRLWKDHFKQLREEQIKQVESQLIMSLWRFLVCQTETVTANEREMRDHRHLVDSLHDLTIKDQASYYEDAFEDLPEYIEEELKMQLNKRTGRIMQVKYDEKFQEMARTILESKSFDLTTLEEADSLYISMGL

Target ORF information:

RefSeq Version	NM_001180807.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Lcd1p (LCD1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180807.3

ORF Insert Sequence:

ATGAGACGAG AAACGGTGGG TGAATTTTCT TCAGATGACG ATGATGATAT TCTTTTGGAA 
TTAGGCACCA GGCCTCCAAG GTTTACTCAA ATACCGCCAT CATCAGCAGC ATTACAAACA 
CAAATTCCCA CTACTTTGGA GGTTACAACG ACCACATTAA ACAATAAACA GAGTAAAAAT 
GATAACCAAC TGGTTAACCA ACTGAATAAA GCTCAAGGTG AAGCAAGCAT GCTTCGTGAT 
AAAATAAACT TTTTGAACAT TGAAAGGGAA AAGGAAAAGA ATATTCAAGC CGTCAAAGTT 
AATGAATTGC AAGTCAAGCA TCTTCAAGAG TTGGCTAAAT TAAAACAAGA ATTACAGAAA 
CTGGAAGATG AGAAGAAGTT CTTACAGATG GAAGCGAGAG GAAAATCGAA AAGGGAAGTT 
ATTACGAATG TAAAACCACC GTCAACAACA TTATCAACAA ACACAAACAC TATAACGCCA 
GATTCGTCCT CAGTTGCAAT CGAAGCAAAA CCTCAATCAC CACAATCAAA AAAACGTAAG 
ATAAGTGATA ATTTACTGAA AAAAAATATG GTTCCCCTAA ACCCAAATAG GATTATTCCC 
GATGAAACGA GTTTATTTCT AGAGTCAATA TTACTTCATC AAATAATAGG CGCTGACCTG 
AGCACAATAG AAATATTAAA TAGATTGAAG CTTGACTACA TCACAGAGTT TAAATTTAAG 
AATTTCGTCA TTGCTAAAGG AGCCCCCATA GGGAAGTCCA TAGTTTCTCT ACTTTTGCGA 
TGTAAAAAGA CGTTGACCCT CGACAGGTTC ATAGATACTT TGCTAGAGGA TATAGCTGTT 
TTGATCAAGG AAATATCAGT TCATCCAAAT GAATCGAAAT TAGCTGTCCC ATTTCTCGTT 
GCATTGATGT ATCAGATTGT ACAATTTCGT CCTAGTGCCA CTCATAATTT AGCACTGAAG 
GATTGTTTTC TTTTTATTTG CGATTTAATA AGAATCTATC ATCATGTATT GAAAGTACCG 
ATACATGAAT CAAATATGAA TTTGCATGTA GAACCTCAGA TTTTCCAATA TGAACTGATA 
GACTATTTGA TAATTTCGTA TTCCTTTGAT CTCCTAGAAG GTATATTAAG GGTACTGCAG 
TCGCATCCTA AGCAAACTTA TATGGAATTT TTTGATGAAA ATATTCTAAA ATCATTTGAA 
TTTGTCTACA AACTAGCACT AACCATTTCA TACAAGCCAA TGGTAAATGT AATATTTAGT 
GCAGTCGAGG TCGTTAATAT TATCACTAGT ATAATATTAA ATATGGACAA TTCCTCAGAT 
CTGAAATCCT TGATAAGCGG TAGTTGGTGG AGAGATTGTA TTACAAGATT ATACGCTCTT 
TTGGAAAAGG AAATTAAGAG TGGCGACGTA TATAATGAAA ATGTGGATAC TACAACTCTT 
CATATGTCGA AGTACCATGA CTTTTTTGGG TTAATCCGAA ATATAGGTGA TAATGAATTG 
GGAGGATTGA TATCAAAGCT GATTTATACT GACCGATTGC AAAGTGTCCC AAGGGTAATT 
TCTAAAGAGG ATATTGGGAT GGATAGCGAC AAATTCACTG CCCCTATAAT AGGTTACAAG 
ATGGAAAAAT GGCTTTTGAA GTTAAAAGAT GAAGTTTTAA ATATTTTTGA AAATTTATTA 
ATGATCTATG GAGACGATGC GACCATAGTA AATGGAGAAA TGCTCATCCA CTCCTCTAAA 
TTCTTATCCA GGGAGCAAGC GTTAATGATA GAAAGGTACG TGGGACAAGA CTCCCCGAAC 
TTGGACCTCA GATGCCATCT TATTGAACAT ACTTTAACCA TAATATATAG GCTATGGAAA 
GACCATTTCA AACAATTGCG TGAAGAACAA ATCAAGCAGG TAGAAAGCCA ATTGATTATG 
TCACTATGGA GGTTTCTCGT ATGCCAAACC GAAACTGTGA CAGCAAACGA AAGAGAAATG 
AGAGATCATC GACACCTTGT AGATAGTTTG CATGATCTGA CGATAAAGGA TCAAGCCTCT 
TATTACGAAG ATGCCTTTGA GGATTTACCA GAATATATCG AAGAAGAATT GAAGATGCAA 
TTGAATAAAA GAACGGGGAG AATAATGCAA GTAAAGTACG ATGAGAAATT TCAAGAAATG 
GCAAGAACTA TTCTTGAGTC AAAATCATTC GATCTAACCA CACTAGAGGA GGCCGATTCA 
TTATATATCT CAATGGGACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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LCD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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