VPS3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS3
Entrez Gene ID	852106
Full Name	CORVET complex subunit VPS3
Synonyms	PEP6,VPL3,VPT17
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180803.3	NP_010783.3	CORVET complex subunit VPS3

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C12353g	XP_452749.1
Saccharomyces cerevisiae (baker's yeast)	VPS3	NP_010783.3
Eremothecium gossypii	AGOS_AFR341C	NP_985888.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C-terminal alpha-helical segments of VPS3 and VPS8 are necessary for CORVET assembly and function.
Title: The N-terminal domains of Vps3 and Vps8 are critical for localization and function of the CORVET tethering complex on endosomes.

Vps3 has an epistatic relationship with Pdr5, a major yeast multidrug transporter.
Title: Pdr5-mediated multidrug resistance requires the CPY-vacuolar sorting protein Vps3: are xenobiotic compounds routed from the vacuole to plasma membrane transporters for efflux?

The following VPS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01562	NM_001180803.3 Latest version!	Saccharomyces cerevisiae S288C CORVET complex subunit VPS3 (VPS3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS3 ( NM_001180803.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010783.3 Saccharomyces cerevisiae S288C CORVET complex subunit VPS3 (VPS3), partial mRNA.

Price & Availability↑

CloneID	OSi01562
Clone ID Related Accession (Same CDS sequence)	NM_001180803.3
Accession Version	NM_001180803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	CORVET complex subunit VPS3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180803.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTAAAAA AGAAAACGAA TAATGACAAA GGAAAAGAAG TAAAGGAAAA TGAAGGAAAG 
CTCGATATAG ATAGCGAATC CTCACCTCAC GAAAGAGAAA ATGACAAAAA AAAAACAGAA 
GATGATAGTC TACGTGCAAC AGAAAGTGAA GAGACGAATA CACATAATGC TAACCCAAAT 
GAAACAGTAC GAGCTGATAA ATTTTCCCAG GAAGAGAGTC GACCAATCGA AGACAGTCCA 
CATACTGATA AAAACACAGC ACAAGAAAGC TGTCAGCCTA GCAGCGCAGA AGATAATGTC 
ATAAATACAG ATATTACAAG CTTAAATGAA AAAACCTCCA CGAATGATGA ACAGGAAAAA 
GGGTTGCCAC TGAAAATAAG CGAAGGTCCT TTTACAATCT CTACCCTATT AGACAATGTA 
CCATCTGACT TGATATACAC ATGTTGTGAG GCGTATGAGA ACCACATATT CTTGGGAACA 
ACTACAGGTG ATCTTTTACA TTACTTTGAA CTTGAACGTG GAAACTATAT GCTTGTGTCG 
CAAACAAAGT TTGATGCAGA ATCAAATTCG AAGATAGACA AAATCCTTCT TTTACCTAAA 
GTAGAAGGCG CACTAATATT ATGTGATAAC GAACTAGTTT TATTTATTCT CCCAGAATTT 
GCACCAAGGC CCAACACAAC TAGACTTAAA GGTATTAGTG ATGTCGTTAT TTGTAATTTT 
TCCCGAAGCT CGAAGGCTTA TAGAATATAT GCATTTCACG CAGAAGGCGT AAGACTACTC 
AAAATATCTG CAGATTCATT AGTACTTACC AAAGCTTTTA ATTTCAAACT AATAGATAAA 
GCTTGTGCGC ACGAAGAAAC ATTAATGGTT TCGAAGTTAA ATAGTTACGA ACTTATAAAT 
CTAAAGTCGT CACAGGTAAT TCCATTATTT CGAATAAGTG AGACTGACGA AGACTTAGAA 
CCTATAATAA CCAGTTTCAA TGAACAGAGT GAATTTTTAG TTTGTTCTGG AGGAGGATCA 
TACGATAGTG GGGCGATGGC CTTAGTGGTA AATCATCATG GCGACATAAT AAAAGGAACT 
ATAGTGCTAA AAAATTATCC AAGAAATGTA ATTGTTGAGT TTCCCTACAT AATCGCTGAG 
TCTGCTTTCC AGTCGGTTGA CATATATTCT GCCTTGCCGA GTGAAAAATC CCAGTTGTTG 
CAAAGTATAA CTACTTCTGG ATCGGATTTA AAAATTTCAA AATCTGACAA TGTATTCACT 
AATACCAATA ATTCTGAAGA ATTCAAAGAA AAAATATTCA ATAAGTTGAG ACTTGAGCCT 
CTGACACATA GTGATAATAA ATTTAGAATT GAAAGAGAAC GCGCCTTTGT TGAAGAGTCT 
TATGAAGAAA AGACCTCTTT AATCGTTTAT AATAATTTAG GGATTCATTT ATTAGTTCCA 
ACGCCGATGG TCTTACGTTT TACATCATGT GAAGAGTCCG AAATTGATAA TATTGAAGAT 
CAATTGAAAA AGCTTGCGAA GAAGGATTTA ACAAAATTTG AGCACATTGA AGCGAAATAT 
TTGATGTCTC TTTTATTGTT TCTAATGACC TTACATTACG ATCACATAGA AGATGAGGTA 
ATGAAAAAGT GGTGTGACTT TTCTGACAAA GTTGACATCA GAATATTATT TTATATGTTT 
GGCTGGAAAG TCTATAGCGA GATCTGGTGC TTCCATGGGT TAATAAATAT AGTTGAAAGG 
TTGAAAAGCT TGAAGCTAAC TAACAAATGC GAGAATATTC TAAAAATGCT TCTGATGATG 
AAGAATGAAC TAAAGAAAAA GAATAAAACT GGACTCTTAA CGAATGACTT TGATGATATA 
ATGAAAACTA TTGATATAAC TTTATTCAAA TTAAGATTGG AAAAAAAGGA AACTATAACT 
GTTGATATGT TTGAACGCGA AAGTTACGAC GAAATTATCA GAGAGATTAA TTTACATGAT 
GATAAACTTC CTAGGATAGA GTTACTAATT GAGATATATA AAGAAAAAGG GGAATATCTT 
AAAGCACTAA ATCTTTTGAG GGAAGCTGGG GATTACATTT CACTAGTTTC ATTCATTGAA 
GAAAATCTCA AAAAACTTCC TGAGGATTAT ATCAAAGAAA GAATAGCCGA TGATCTTCTC 
CTCACTCTTA AACAAGGCGA CGAAAATACT GAGGAATGCG CCATCAAAAA GGTTCTTAAA 
ATATTGGACA TGGCATGTAT TAATAAAAAC GACTTTTTAA ACAAAATACC CGCAGAAGAA 
ACATCCCTGA AGGTATCATT CATAGAACAA CTCGGTGTCC AAAACAGCAA CGATTCCAAA 
TTTCTCTTTA ACTATTATTT GGCGAAATTG CGAGAAATAA TCAACCAAAG TAACATATGG 
TCCATTCTTG GAGATTTTAT CAAAGAGTAC AAAGACGATT TCGCGTATGA TAAGACAGAT 
ATAACAAATT TTATTCATAT CAAATTAAAG CATAGTCTTC AGTGCGAAAA TTTTTCCAAA 
TATTATGAAA AATGTGAAAA CTTGAAATCG GAAAATGAAA AAGATGATGA GTTTATTAAT 
TTCACCTTCG ACGAAATTTC CAAGATCGAT AAAGAACACA TATTGACCTT GTTATTTTTC 
CCCAATGAAT TAACAAACTG GGTTTCTTCC GAAGAATTGC TGAAAATATA TTTGTCATTT 
AATGATTTCA GAAGTGTAGA AAAATACATA GGCAAGCAGA ACTTGGTTGC GGTGATGAAA 
CAATACTTGG ACATATCTTC TTTGAATTAC TCAGTTGAAT TAGTGACTAA TTTGCTACAG 
AGAAATTTTG AACTTTTAGA TGATACAGAT ATTCAATTAA AGATCCTCGA AACTATACCT 
TCCGTATTTC CCGTACAAAC AATATCCGAA TTGCTTTTAA AGGTACTTAT TAAGTATCAA 
GAAAAGAAGG AGGAATCTAA TTTGCGGAAA TGTTTGTTGA AAAATCAAAT ATCCATTTCT 
GACGAACTAA GCCGGAATTT TGATAGTCAA GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010783.3
CDS	1..3036
Translation	MVKKKTNNDKGKEVKENEGKLDIDSESSPHERENDKKKTEDDSLRATESEETNTHNANPNETVRADKFSQEESRPIEDSPHTDKNTAQESCQPSSAEDNVINTDITSLNEKTSTNDEQEKGLPLKISEGPFTISTLLDNVPSDLIYTCCEAYENHIFLGTTTGDLLHYFELERGNYMLVSQTKFDAESNSKIDKILLLPKVEGALILCDNELVLFILPEFAPRPNTTRLKGISDVVICNFSRSSKAYRIYAFHAEGVRLLKISADSLVLTKAFNFKLIDKACAHEETLMVSKLNSYELINLKSSQVIPLFRISETDEDLEPIITSFNEQSEFLVCSGGGSYDSGAMALVVNHHGDIIKGTIVLKNYPRNVIVEFPYIIAESAFQSVDIYSALPSEKSQLLQSITTSGSDLKISKSDNVFTNTNNSEEFKEKIFNKLRLEPLTHSDNKFRIERERAFVEESYEEKTSLIVYNNLGIHLLVPTPMVLRFTSCEESEIDNIEDQLKKLAKKDLTKFEHIEAKYLMSLLLFLMTLHYDHIEDEVMKKWCDFSDKVDIRILFYMFGWKVYSEIWCFHGLINIVERLKSLKLTNKCENILKMLLMMKNELKKKNKTGLLTNDFDDIMKTIDITLFKLRLEKKETITVDMFERESYDEIIREINLHDDKLPRIELLIEIYKEKGEYLKALNLLREAGDYISLVSFIEENLKKLPEDYIKERIADDLLLTLKQGDENTEECAIKKVLKILDMACINKNDFLNKIPAEETSLKVSFIEQLGVQNSNDSKFLFNYYLAKLREIINQSNIWSILGDFIKEYKDDFAYDKTDITNFIHIKLKHSLQCENFSKYYEKCENLKSENEKDDEFINFTFDEISKIDKEHILTLLFFPNELTNWVSSEELLKIYLSFNDFRSVEKYIGKQNLVAVMKQYLDISSLNYSVELVTNLLQRNFELLDDTDIQLKILETIPSVFPVQTISELLLKVLIKYQEKKEESNLRKCLLKNQISISDELSRNFDSQG

Target ORF information:

RefSeq Version	NM_001180803.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C CORVET complex subunit VPS3 (VPS3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180803.3

ORF Insert Sequence:

ATGGTAAAAA AGAAAACGAA TAATGACAAA GGAAAAGAAG TAAAGGAAAA TGAAGGAAAG 
CTCGATATAG ATAGCGAATC CTCACCTCAC GAAAGAGAAA ATGACAAAAA AAAAACAGAA 
GATGATAGTC TACGTGCAAC AGAAAGTGAA GAGACGAATA CACATAATGC TAACCCAAAT 
GAAACAGTAC GAGCTGATAA ATTTTCCCAG GAAGAGAGTC GACCAATCGA AGACAGTCCA 
CATACTGATA AAAACACAGC ACAAGAAAGC TGTCAGCCTA GCAGCGCAGA AGATAATGTC 
ATAAATACAG ATATTACAAG CTTAAATGAA AAAACCTCCA CGAATGATGA ACAGGAAAAA 
GGGTTGCCAC TGAAAATAAG CGAAGGTCCT TTTACAATCT CTACCCTATT AGACAATGTA 
CCATCTGACT TGATATACAC ATGTTGTGAG GCGTATGAGA ACCACATATT CTTGGGAACA 
ACTACAGGTG ATCTTTTACA TTACTTTGAA CTTGAACGTG GAAACTATAT GCTTGTGTCG 
CAAACAAAGT TTGATGCAGA ATCAAATTCG AAGATAGACA AAATCCTTCT TTTACCTAAA 
GTAGAAGGCG CACTAATATT ATGTGATAAC GAACTAGTTT TATTTATTCT CCCAGAATTT 
GCACCAAGGC CCAACACAAC TAGACTTAAA GGTATTAGTG ATGTCGTTAT TTGTAATTTT 
TCCCGAAGCT CGAAGGCTTA TAGAATATAT GCATTTCACG CAGAAGGCGT AAGACTACTC 
AAAATATCTG CAGATTCATT AGTACTTACC AAAGCTTTTA ATTTCAAACT AATAGATAAA 
GCTTGTGCGC ACGAAGAAAC ATTAATGGTT TCGAAGTTAA ATAGTTACGA ACTTATAAAT 
CTAAAGTCGT CACAGGTAAT TCCATTATTT CGAATAAGTG AGACTGACGA AGACTTAGAA 
CCTATAATAA CCAGTTTCAA TGAACAGAGT GAATTTTTAG TTTGTTCTGG AGGAGGATCA 
TACGATAGTG GGGCGATGGC CTTAGTGGTA AATCATCATG GCGACATAAT AAAAGGAACT 
ATAGTGCTAA AAAATTATCC AAGAAATGTA ATTGTTGAGT TTCCCTACAT AATCGCTGAG 
TCTGCTTTCC AGTCGGTTGA CATATATTCT GCCTTGCCGA GTGAAAAATC CCAGTTGTTG 
CAAAGTATAA CTACTTCTGG ATCGGATTTA AAAATTTCAA AATCTGACAA TGTATTCACT 
AATACCAATA ATTCTGAAGA ATTCAAAGAA AAAATATTCA ATAAGTTGAG ACTTGAGCCT 
CTGACACATA GTGATAATAA ATTTAGAATT GAAAGAGAAC GCGCCTTTGT TGAAGAGTCT 
TATGAAGAAA AGACCTCTTT AATCGTTTAT AATAATTTAG GGATTCATTT ATTAGTTCCA 
ACGCCGATGG TCTTACGTTT TACATCATGT GAAGAGTCCG AAATTGATAA TATTGAAGAT 
CAATTGAAAA AGCTTGCGAA GAAGGATTTA ACAAAATTTG AGCACATTGA AGCGAAATAT 
TTGATGTCTC TTTTATTGTT TCTAATGACC TTACATTACG ATCACATAGA AGATGAGGTA 
ATGAAAAAGT GGTGTGACTT TTCTGACAAA GTTGACATCA GAATATTATT TTATATGTTT 
GGCTGGAAAG TCTATAGCGA GATCTGGTGC TTCCATGGGT TAATAAATAT AGTTGAAAGG 
TTGAAAAGCT TGAAGCTAAC TAACAAATGC GAGAATATTC TAAAAATGCT TCTGATGATG 
AAGAATGAAC TAAAGAAAAA GAATAAAACT GGACTCTTAA CGAATGACTT TGATGATATA 
ATGAAAACTA TTGATATAAC TTTATTCAAA TTAAGATTGG AAAAAAAGGA AACTATAACT 
GTTGATATGT TTGAACGCGA AAGTTACGAC GAAATTATCA GAGAGATTAA TTTACATGAT 
GATAAACTTC CTAGGATAGA GTTACTAATT GAGATATATA AAGAAAAAGG GGAATATCTT 
AAAGCACTAA ATCTTTTGAG GGAAGCTGGG GATTACATTT CACTAGTTTC ATTCATTGAA 
GAAAATCTCA AAAAACTTCC TGAGGATTAT ATCAAAGAAA GAATAGCCGA TGATCTTCTC 
CTCACTCTTA AACAAGGCGA CGAAAATACT GAGGAATGCG CCATCAAAAA GGTTCTTAAA 
ATATTGGACA TGGCATGTAT TAATAAAAAC GACTTTTTAA ACAAAATACC CGCAGAAGAA 
ACATCCCTGA AGGTATCATT CATAGAACAA CTCGGTGTCC AAAACAGCAA CGATTCCAAA 
TTTCTCTTTA ACTATTATTT GGCGAAATTG CGAGAAATAA TCAACCAAAG TAACATATGG 
TCCATTCTTG GAGATTTTAT CAAAGAGTAC AAAGACGATT TCGCGTATGA TAAGACAGAT 
ATAACAAATT TTATTCATAT CAAATTAAAG CATAGTCTTC AGTGCGAAAA TTTTTCCAAA 
TATTATGAAA AATGTGAAAA CTTGAAATCG GAAAATGAAA AAGATGATGA GTTTATTAAT 
TTCACCTTCG ACGAAATTTC CAAGATCGAT AAAGAACACA TATTGACCTT GTTATTTTTC 
CCCAATGAAT TAACAAACTG GGTTTCTTCC GAAGAATTGC TGAAAATATA TTTGTCATTT 
AATGATTTCA GAAGTGTAGA AAAATACATA GGCAAGCAGA ACTTGGTTGC GGTGATGAAA 
CAATACTTGG ACATATCTTC TTTGAATTAC TCAGTTGAAT TAGTGACTAA TTTGCTACAG 
AGAAATTTTG AACTTTTAGA TGATACAGAT ATTCAATTAA AGATCCTCGA AACTATACCT 
TCCGTATTTC CCGTACAAAC AATATCCGAA TTGCTTTTAA AGGTACTTAT TAAGTATCAA 
GAAAAGAAGG AGGAATCTAA TTTGCGGAAA TGTTTGTTGA AAAATCAAAT ATCCATTTCT 
GACGAACTAA GCCGGAATTT TGATAGTCAA GGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VPS3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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