ORF » Species Summary » Saccharomyces cerevisiae S288C » DOT1 cDNA ORF clone

DOT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following DOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01513 NM_001180748.1
Latest version!		 Saccharomyces cerevisiae S288C histone methyltransferase DOT1 (DOT1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    DOT1 ( NM_001180748.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010728.1 Saccharomyces cerevisiae S288C histone methyltransferase DOT1 (DOT1), partial mRNA.
    Price & Availability↑
    CloneID OSi01513
    Clone ID Related Accession (Same CDS sequence) NM_001180748.1
    Accession Version NM_001180748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1749bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product histone methyltransferase DOT1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGCGGTC AAGAAAGTAT ATCAAATAAT AACTCAGACT CATTCATTAT GTCGTCCCCC 
    AACTTAGACT CTCAGGAATC TTCAATATCA CCTATTGATG AGAAGAAAGG CACCGATATG 
    CAAACCAAGT CGCTTTCAAG CTATTCTAAA GGTACGCTTC TCTCTAAGCA AGTACAAAAT 
    TTATTAGAAG AAGCTAATAA ATACGATCCA ATATATGGGT CATCTTTACC TCGAGGATTT 
    TTAAGAGATA GAAACACCAA GGGTAAGGAT AATGGGTTGG TTCCGCTGGT GGAGAAGGTT 
    ATACCTCCCA TTCACAAGAA AACCAATAAT AGAAACACAA GGAAGAAGTC ATCTACCACC 
    ACGAAGAAGG ATGTAAAGAA GCCAAAAGCT GCAAAAGTAA AAGGAAAAAA TGGCAGGACT 
    AACCATAAAC ATACCCCAAT TTCAAAGCAA GAAATAGATA CTGCACGAGA AAAAAAGCCA 
    TTGAAAAAAG GTCGGGCAAA CAAGAAGAAT GATCGCGATA GTCCTTCATC AACATTTGTT 
    GATTGGAATG GCCCGTGTCT ACGGTTACAA TATCCATTAT TTGACATAGA GTACTTAAGA 
    TCACATGAAA TATATTCTGG AACTCCTATA CAATCCATTA GTTTAAGAAC AAATTCTCCA 
    CAGCCAACGA GCTTGACATC AGATAACGAC ACTTCCTCAG TAACGACAGC AAAGTTGCAG 
    AGTATTTTAT TTTCAAATTA TATGGAAGAG TACAAAGTCG ACTTCAAAAG GTCAACAGCC 
    ATTTATAATC CAATGAGTGA AATTGGTAAA TTAATTGAAT ACAGCTGCCT GGTCTTTTTA 
    CCTTCACCTT ATGCTGAACA ATTGAAGGAA ACTATACTAC CGGACCTAAA TGCATCATTT 
    GATAACTCTG ACACGAAAGG TTTCGTGAAT GCTATAAATT TATACAACAA AATGATTCGT 
    GAAATTCCTA GGCAAAGAAT AATTGACCAT TTAGAAACAA TTGATAAAAT TCCTCGTTCA 
    TTCATTCATG ACTTCTTGCA TATCGTCTAT ACCAGGAGTA TCCATCCGCA GGCGAATAAA 
    TTGAAACATT ACAAAGCATT CAGCAATTAT GTTTATGGAG AACTTTTGCC CAATTTCCTA 
    TCTGATGTAT ATCAACAATG CCAGTTGAAG AAGGGTGACA CTTTCATGGA TCTCGGTTCG 
    GGAGTAGGTA ATTGCGTAGT ACAAGCTGCG TTGGAATGTG GATGTGCATT AAGCTTCGGA 
    TGTGAAATCA TGGATGATGC TAGCGATTTA ACTATACTGC AGTACGAGGA ACTAAAGAAG 
    AGGTGTAAGT TATATGGGAT GCGTTTGAAC AACGTGGAGT TTTCATTGAA GAAAAGCTTT 
    GTGGACAATA ACAGGGTCGC TGAACTAATT CCTCAGTGCG ATGTTATCCT CGTAAATAAT 
    TTTTTATTTG ATGAAGATTT GAATAAAAAA GTCGAAAAGA TACTACAAAC GGCAAAAGTT 
    GGATGTAAGA TCATAAGTTT GAAAAGTTTA AGAAGCCTCA CTTATCAGAT CAACTTCTAC 
    AATGTTGAGA ACATCTTCAA TAGATTAAAG GTGCAAAGGT ATGATCTTAA GGAGGATAGT 
    GTTTCATGGA CGCATAGTGG CGGAGAGTAT TATATATCAA CAGTGATGGA GGATGTGGAC 
    GAAAGTTTAT TCAGCCCTGC TGCAAGAGGT AGGAGGAACA GAGGTACGCC GGTGAAGTAT 
    ACCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010728.1
    CDS1..1749
    Translation

    Target ORF information:

    RefSeq Version NM_001180748.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C histone methyltransferase DOT1 (DOT1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001180748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGCGGTC AAGAAAGTAT ATCAAATAAT AACTCAGACT CATTCATTAT GTCGTCCCCC 
    AACTTAGACT CTCAGGAATC TTCAATATCA CCTATTGATG AGAAGAAAGG CACCGATATG 
    CAAACCAAGT CGCTTTCAAG CTATTCTAAA GGTACGCTTC TCTCTAAGCA AGTACAAAAT 
    TTATTAGAAG AAGCTAATAA ATACGATCCA ATATATGGGT CATCTTTACC TCGAGGATTT 
    TTAAGAGATA GAAACACCAA GGGTAAGGAT AATGGGTTGG TTCCGCTGGT GGAGAAGGTT 
    ATACCTCCCA TTCACAAGAA AACCAATAAT AGAAACACAA GGAAGAAGTC ATCTACCACC 
    ACGAAGAAGG ATGTAAAGAA GCCAAAAGCT GCAAAAGTAA AAGGAAAAAA TGGCAGGACT 
    AACCATAAAC ATACCCCAAT TTCAAAGCAA GAAATAGATA CTGCACGAGA AAAAAAGCCA 
    TTGAAAAAAG GTCGGGCAAA CAAGAAGAAT GATCGCGATA GTCCTTCATC AACATTTGTT 
    GATTGGAATG GCCCGTGTCT ACGGTTACAA TATCCATTAT TTGACATAGA GTACTTAAGA 
    TCACATGAAA TATATTCTGG AACTCCTATA CAATCCATTA GTTTAAGAAC AAATTCTCCA 
    CAGCCAACGA GCTTGACATC AGATAACGAC ACTTCCTCAG TAACGACAGC AAAGTTGCAG 
    AGTATTTTAT TTTCAAATTA TATGGAAGAG TACAAAGTCG ACTTCAAAAG GTCAACAGCC 
    ATTTATAATC CAATGAGTGA AATTGGTAAA TTAATTGAAT ACAGCTGCCT GGTCTTTTTA 
    CCTTCACCTT ATGCTGAACA ATTGAAGGAA ACTATACTAC CGGACCTAAA TGCATCATTT 
    GATAACTCTG ACACGAAAGG TTTCGTGAAT GCTATAAATT TATACAACAA AATGATTCGT 
    GAAATTCCTA GGCAAAGAAT AATTGACCAT TTAGAAACAA TTGATAAAAT TCCTCGTTCA 
    TTCATTCATG ACTTCTTGCA TATCGTCTAT ACCAGGAGTA TCCATCCGCA GGCGAATAAA 
    TTGAAACATT ACAAAGCATT CAGCAATTAT GTTTATGGAG AACTTTTGCC CAATTTCCTA 
    TCTGATGTAT ATCAACAATG CCAGTTGAAG AAGGGTGACA CTTTCATGGA TCTCGGTTCG 
    GGAGTAGGTA ATTGCGTAGT ACAAGCTGCG TTGGAATGTG GATGTGCATT AAGCTTCGGA 
    TGTGAAATCA TGGATGATGC TAGCGATTTA ACTATACTGC AGTACGAGGA ACTAAAGAAG 
    AGGTGTAAGT TATATGGGAT GCGTTTGAAC AACGTGGAGT TTTCATTGAA GAAAAGCTTT 
    GTGGACAATA ACAGGGTCGC TGAACTAATT CCTCAGTGCG ATGTTATCCT CGTAAATAAT 
    TTTTTATTTG ATGAAGATTT GAATAAAAAA GTCGAAAAGA TACTACAAAC GGCAAAAGTT 
    GGATGTAAGA TCATAAGTTT GAAAAGTTTA AGAAGCCTCA CTTATCAGAT CAACTTCTAC 
    AATGTTGAGA ACATCTTCAA TAGATTAAAG GTGCAAAGGT ATGATCTTAA GGAGGATAGT 
    GTTTCATGGA CGCATAGTGG CGGAGAGTAT TATATATCAA CAGTGATGGA GGATGTGGAC 
    GAAAGTTTAT TCAGCCCTGC TGCAAGAGGT AGGAGGAACA GAGGTACGCC GGTGAAGTAT 
    ACCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
    Nature387(6632 Suppl)75-8(1997 May)
    Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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