HKR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HKR1
Entrez Gene ID	852030
Full Name	Hkr1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180728.1	NP_010708.1	Hkr1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interaction between Ste20 and Sho1 contributed to the activation of Hog1 by Hkr1, but not by Msb2.
Title: Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms.

The osmosensor Hkr1 is required for activation of the yeast cell wall integrity MAPK pathway signaling but Msb2 is not.
Title: Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2.

Data showed that Msb2p had a function in cell polarization through the adaptor protein Sho1p that Hkr1p did not.
Title: The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response.

Hkr1 and Msb2 individually form a complex with Sho1, and, upon high external osmolarity stress, appear to induce Sho1 to generate an intracellular signal
Title: Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway.

The following HKR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HKR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01496	NM_001180728.1 Latest version!	Saccharomyces cerevisiae S288C Hkr1p (HKR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HKR1 ( NM_001180728.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010708.1 Saccharomyces cerevisiae S288C Hkr1p (HKR1), partial mRNA.

Price & Availability↑

CloneID	OSi01496
Clone ID Related Accession (Same CDS sequence)	NM_001180728.1
Accession Version	NM_001180728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Hkr1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTCTCAT TGAAAATAAA AAAAATTTTA CTCCTGGTGT CATTGTTAAA TGCAATCGAG 
GCCTATAGTA ACGATACAAT ATATTCAACT TCATACAATA ATGGAATAGA AAGCACACCC 
TCATATTCAA CATCCGCGAT ATCCAGTACC GGATCTAGCA ACAAAGAGAA TGCAATAACA 
TCAAGCTCTG AAACCACCAC AATGGCTGGC CAATATGGTG AAAGTGGAAG CACAACAATA 
ATGGATGAAC AAGAAACTGG TACGTCCAGC CAGTATATTA GTGTGACGAC GACAACGCAA 
ACTTCTGACA CGATGTCATC AGTAAAAAAG TCTACGGAAA TAGCGACACC AAGTAGTAGC 
ATTGTACCCA CCCCTTTGCA GTCCTATAGC GATGAATCAC AAATATCACA AACGCTTAGT 
CACAATCCGA AGTCCGTGGC GGAGTCAGAT AGCGATACAA CCTCCTCGGA ATCCTCAAGT 
AGCGTAATAA TATCCACATC CGATAGTTCA GCGGTACCGA GGGAAATAAG TCCAATAATA 
ACCACAGATT CTCAAATTTC CAAAGAAGAA GGCACATTGG CTCAAACTTC CAGTATCAGT 
GAAACAACGA GAATTGCACA AATGGTAACC AGAGTTTCAC AAATCTCCAG CATAACGGCA 
GCTTCAACAA TAGATGGCTT TAGTAGTGAA TCAACACAAA CAGATTTTTC CAACACTGTA 
TCGTTTGAAA ATTCAGTCGA AGAAGAATAT GCCATGAGCA AATCCCAGCT CTCTGAAAGT 
TACAGTTCAA GTTCAACCGT CTATTCTGGA GGGGAGTCGA CTGCAGACAA GACATCTAGC 
TCCCCCATTA CCAGCTTTTC ATCTTCTTAT AGCCAAACAA CGTCAACTGA AACCTCTGAG 
AGCAGCCGAG TGGCTGTTGG AGTATCCCGG CCCTCTAGCA TAACGCAAAC TACATCAATT 
GATAGTTTTA GTATGAGTGA AGTAGAGCTT TCCACATATT ATGACTTAAG TGCTGGCAAC 
TATCCTGACC AAGAACTGAT TGTCGATCGC CCGGCCACTT CTTCCACTGC CGAAACATCT 
TCAGAGGCAA GTCAAGGTGT AAGCCGTGAA TCTAACACTT TCGCAGTATC TTCGATTTCC 
ACGACAAACT TTATAGTTAG CAGTGCTTCG GATACTGTTG TTTCAACTTC AAGCACCAAT 
ACAGTGCCAT ACAGCTCTGT CCATTCAACC TTTGTTCATG CGACTTCCTC GTCGACTTAT 
ATTTCTTCTT CCCTTTATTC TTCCCCATCC TTATCTGCAT CCGTTTCCTC TCACTTTGGC 
GTTGCTCCTT TTCCATCTGC TTACATATCT TTCTCATCAG TACCCGTTGC AGTATCCTCG 
ACGTACACGT CTTCACCATC GGCTTCTGTT GTAGTACCTT CTGCCTATGC GTCTTCACCA 
TCAGTACCCG TTGCAGTATC CTCGACGTAC ACGTCTTCAC CATCAGCACC TGCTGCAATA 
TCCTCAACGT ACACGTCTTC ACCATCGGCA CCTGTTGCAG TATCCTCGAC GTACACGTCT 
TCACCATCGG CACCTGCTGC AATATCCTCA ACGTACACGT CTTCACCATC GGCACCTGTT 
GCAGTATCCT CGACGTACAC GTCTTCACCA TCAGCACCTG CTGCAATATC CTCAACGTAC 
ACGTCTTCAC CATCGGCACC TGTTGCAGTA TCCTCGACGT ACACGTCTTC ACCATCAGCA 
CCTGTTGCAA TATCCTCAAC GTACACGTCT TCACCATCGG TACCTGTTGC AGTATCCTCG 
ACGTACACGT CTTCACCATC AGCACCTGCT GCAATATCCT CAACGTACAC GTCTTCACCA 
TCGGCACCCG TTGCAGTATC CTCGACGTAC ACGTCTTCAC CATCAGCACC TGCTGCAATA 
TCCTCAACGT ACACGTCTTC ACCATCAGTA CCCGTTGCAG TATCCTCGAC GTACACGTCT 
TCACCATCAG CACCTGCTGC AATATCCTCA ACGTACACGT CTTCACCATC AGTACCCGTT 
GCAGTATCCT CGACGTACAC GTCTTCACCA TCAGCACCTG CTGCAATATC CTCAACGTAC 
ACGTCTTCAC CATCGGCACC CGTTGCAGTA TCCTCGACGT ACACGTCTTC ACCATCAGCA 
CCTGCTGCAA TATCCTCAAC GTACACGTCT TCACCATCGG CACCCGTTGC AGTATCCTCG 
ACGTACACGT CTTCACCATC AGCACCTGCT GCAATATCCT CAACGTACAC GTCTTCACCA 
TCGGCACCTG TTGCAGTATC CTCGACGTAC ACGTCTTCAC CATCGGCTCT TGTTGTCCTG 
TCATCTACTT CCACATCCTC TCCATATGAT ATTGTTTACT CTCCATCAAC TTTTGCTGCC 
ATATCATCCG GTTACACGCC TTCACCTTCT GCTTCTGTCG CCATGTCATC TACTTCCTCA 
TCCTCTCCAT ATGATATTGT TTACTCTCTA TCATCTTCTG CTTCCAGATC GTCCATTGCT 
ACATATGAAT TTTCACCTTC CCCATCTACT TCTTTACCCA CCTCTTCTAC TTACACATAC 
TTTTCATCTG CTTATGCGTT TGAATTTTCT TCTGAGCGGT ACTCAACAAC ATCCACAATA 
GCACCAACTC AAATACATAG CACATTGTCC AGGATTACGG ATTTCTTACT GCAGACTTCG 
ATGGCAATCC AAAGTATAGT CTCTCAGCAA ATTTCTACAA GCTCAACTTT AAATGACGAA 
ATACATTCAA GCGCGCTTTC CGTTTTCAAC CCTTCAGCTT CGAATTTGGT TGAAACTTCT 
CTGATAATTT CATCGACCCA AGCAAGCATA ACAAGCCCCA AAAACTCAGC TAAAATTAGT 
TCACTACAGA GCCAGCTGTC TAGCAGTACA AAAAATCCAT ACGATACGGC GAACAAGAAT 
ACTGAGACCA GTGGCAGATC CACAGTTGTT TCGAATTTCC TATACACATC TTCCGCGGCG 
AAACCAGACA ATGAAAAGTT TTCCGCTACT CCTACAGAGA TAACTACAAT TTCAAGTAGC 
TCGCACGCAT ATTCTTTATC TATTCCAAGT TCTCATAATT CTGTGACTGG ACTATCACAT 
AACTTTGTCG ATTCGTCAAA AAGTGCAACC TCATTTGGCT ACTCATCCTC ATCAATATCT 
TCAATAAAAC TTTCAAAAGA AACAATACCT GCAAGTAAAT CTGTTTCAAA CACACAAGAA 
AGAATCACAA GCTTTACATC AACATTACGA GCAAATAGTC AGTCTGAAAA ATCCGAAGGG 
AGGAATTCTG TAGGTTCTTT ACAAAGTTCA CATATTTCAT CTAACCCATC GCTCTCCACA 
AATACAAAGG TTGATTCGAA AAGTTTATCC AGGAAAGTAT CGAAAACCAT GGGCGAAAAT 
GGCGAGGAAA CCGGCTTAAC CACAACAAAG ACGCAATACA AAAGTTCTTC TGAAACCAGT 
GGGTCATATT CTCGGTCTTT TACAAAAATC TCAATTGGAC CTGCTACGAC TGCAGTTCAA 
ACTCAGGCAA GCACTAATTC AGTATTCACA GCCCCTGCCT TGTCTACCTA TCCTACTACT 
CCGTACCCCT CTCCAAATAG TTACGCATGG TTACCTACCG CTATTATAGT GGAATCATCC 
GAAACGGGCC CCACTACCGC ATCATTCAAT CCGTCAATCA CTGGTTCACT TCCCAATGCA 
ATTGAACCGG CCGTCGCTGT ATCGGAACCC ATTAACCACA CTTTAATTAC CATCGGATTC 
ACGGCCGCTT TGAATTATGT ATTTTTGGTT CAAAATCCAT TATCTTCGGC TCAAATCTTC 
AACTTTTTAC CTCTCGTTTT GAAGTATCCC TTTTCAAACA CCTCATCAGA ACTGGACAAT 
AGTATTGGGG AATTATCCAC TTTTATTCTT TCATACCGGT CTGGGAGCAG CACAACTACC 
CTTTCCCCAA AATCCATATC ATCATTATCA GTTGTCAAGA AAAAAAAAAA CCAGCAGAAA 
AAAAATGCCA CGAAGTCAAC CGAGGACTTA CATCCTCCAC AAGTCGACAC ATCCTCAATA 
GCGGTGAAAA AGATTGTTCC CATGGTAGAC TCTTCTAAGG CATACATTGT ATCAGTTGCA 
GAGGTATATT TTCCAACAGA AGCCGTAACA TATCTTCAGC AATTAATCCT TGATGAAAAT 
TCGACTCTTT ATAGCAATCC TCAAACCCCA CTGCGAAGTT TAGCTGGTCT AATAGATAGT 
GGGATCCCAT TAGGGGGATT GACGTTATAT GGCTCTGGAG ATGGTGGTTA TGTCCCTAGT 
TTAACCTCAT CAAGCGTCTT GGATTCTTCA AAAGGAAATT CCCAAAACAT AGATGGAACT 
TACAAGTATG GAGCTTTGGA TGACTTTATC AATTCATTCA CAGATTCTGC ATCTGCAGGA 
AAGTATGCTG TAAAAATAAT CATCTTTTTG ATTGTTTTAA CTATTGGCGT ACTCTTGTGG 
TTATTCGTTG CATTTTTTGC TTTCCGTCAT AGAAATATAT TATTGAAAAG ACACCCAAGG 
AACTGCATAG GGAAAAGCCT CAATAATGAG AGGGAACTTG AAAGTACCGA GCTTTCCCGC 
TCTTCTTCTG GAAACCAAGT TTATAATGAA AAGCCGCCAG AATCTGAAAA TGAAAGTGTT 
TACTCTGCGG TAGATGATCA TTATATTGTG ACTGGCGAAA ACACAGTCTA TAATACTATT 
CACAGATTAC ATTATACCAT TAATGATGAC GGTGATCTAT TGTACAGGGA CGCAATCCCC 
CTCGATTTTG ATCAAACCAA TGGGGATGAT GGTTCGGGAA TAGACAGTAT AGTAAGAGAC 
TGCGTGTATG ACAAAAACCA AGATGCTACG GAAGCCTTCT TGAATGATGA GGAGTCCATA 
TCAGGCATTC TAGATGTTGA TGAAAATGGT GATATTCGAT TATACGACAG TTATTCTGAC 
AATGAAGAGT CAAACAGTTT TCACTTGCCA GACGAAGTTA TTGAAAACTA TAACAAGAAC 
CATTTGTGCG AAACTAAACT TCATGGACTA GGCACTGAAT CATGTACTAC AGATGATCCC 
GATACTGGAA ATCAAATTAC GAATGAGTTT TCTACAGGGA GTCAGACATG TTTGCCAAGT 
ACCGCTTATA CCACACCCCT TCATACAAAT TCGATTAAAC TTCACACATT ACGCTACACA 
GAATCTTCAC TACCAAAGCC AAATCAGACT CTTTTCTCTA ATCTTGAAGA TTTAGAAATA 
GAGGATATTG ACGACAATGG TAGTGTTTCA GATGTACATA TCGAGGAGCT GGATGCTTTA 
GACGAAGAAC TTTATAAGAG AATGTCGAAA GTTATAAAAC AACAGAACCA TCAAACGACA 
AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010708.1
CDS	1..5409
Translation	MVSLKIKKILLLVSLLNAIEAYSNDTIYSTSYNNGIESTPSYSTSAISSTGSSNKENAITSSSETTTMAGQYGESGSTTIMDEQETGTSSQYISVTTTTQTSDTMSSVKKSTEIATPSSSIVPTPLQSYSDESQISQTLSHNPKSVAESDSDTTSSESSSSVIISTSDSSAVPREISPIITTDSQISKEEGTLAQTSSISETTRIAQMVTRVSQISSITAASTIDGFSSESTQTDFSNTVSFENSVEEEYAMSKSQLSESYSSSSTVYSGGESTADKTSSSPITSFSSSYSQTTSTETSESSRVAVGVSRPSSITQTTSIDSFSMSEVELSTYYDLSAGNYPDQELIVDRPATSSTAETSSEASQGVSRESNTFAVSSISTTNFIVSSASDTVVSTSSTNTVPYSSVHSTFVHATSSSTYISSSLYSSPSLSASVSSHFGVAPFPSAYISFSSVPVAVSSTYTSSPSASVVVPSAYASSPSVPVAVSSTYTSSPSAPAAISSTYTSSPSAPVAVSSTYTSSPSAPAAISSTYTSSPSAPVAVSSTYTSSPSAPAAISSTYTSSPSAPVAVSSTYTSSPSAPVAISSTYTSSPSVPVAVSSTYTSSPSAPAAISSTYTSSPSAPVAVSSTYTSSPSAPAAISSTYTSSPSVPVAVSSTYTSSPSAPAAISSTYTSSPSVPVAVSSTYTSSPSAPAAISSTYTSSPSAPVAVSSTYTSSPSAPAAISSTYTSSPSAPVAVSSTYTSSPSAPAAISSTYTSSPSAPVAVSSTYTSSPSALVVLSSTSTSSPYDIVYSPSTFAAISSGYTPSPSASVAMSSTSSSSPYDIVYSLSSSASRSSIATYEFSPSPSTSLPTSSTYTYFSSAYAFEFSSERYSTTSTIAPTQIHSTLSRITDFLLQTSMAIQSIVSQQISTSSTLNDEIHSSALSVFNPSASNLVETSLIISSTQASITSPKNSAKISSLQSQLSSSTKNPYDTANKNTETSGRSTVVSNFLYTSSAAKPDNEKFSATPTEITTISSSSHAYSLSIPSSHNSVTGLSHNFVDSSKSATSFGYSSSSISSIKLSKETIPASKSVSNTQERITSFTSTLRANSQSEKSEGRNSVGSLQSSHISSNPSLSTNTKVDSKSLSRKVSKTMGENGEETGLTTTKTQYKSSSETSGSYSRSFTKISIGPATTAVQTQASTNSVFTAPALSTYPTTPYPSPNSYAWLPTAIIVESSETGPTTASFNPSITGSLPNAIEPAVAVSEPINHTLITIGFTAALNYVFLVQNPLSSAQIFNFLPLVLKYPFSNTSSELDNSIGELSTFILSYRSGSSTTTLSPKSISSLSVVKKKKNQQKKNATKSTEDLHPPQVDTSSIAVKKIVPMVDSSKAYIVSVAEVYFPTEAVTYLQQLILDENSTLYSNPQTPLRSLAGLIDSGIPLGGLTLYGSGDGGYVPSLTSSSVLDSSKGNSQNIDGTYKYGALDDFINSFTDSASAGKYAVKIIIFLIVLTIGVLLWLFVAFFAFRHRNILLKRHPRNCIGKSLNNERELESTELSRSSSGNQVYNEKPPESENESVYSAVDDHYIVTGENTVYNTIHRLHYTINDDGDLLYRDAIPLDFDQTNGDDGSGIDSIVRDCVYDKNQDATEAFLNDEESISGILDVDENGDIRLYDSYSDNEESNSFHLPDEVIENYNKNHLCETKLHGLGTESCTTDDPDTGNQITNEFSTGSQTCLPSTAYTTPLHTNSIKLHTLRYTESSLPKPNQTLFSNLEDLEIEDIDDNGSVSDVHIEELDALDEELYKRMSKVIKQQNHQTTKI

Target ORF information:

RefSeq Version	NM_001180728.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Hkr1p (HKR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180728.1

ORF Insert Sequence:

ATGGTCTCAT TGAAAATAAA AAAAATTTTA CTCCTGGTGT CATTGTTAAA TGCAATCGAG 
GCCTATAGTA ACGATACAAT ATATTCAACT TCATACAATA ATGGAATAGA AAGCACACCC 
TCATATTCAA CATCCGCGAT ATCCAGTACC GGATCTAGCA ACAAAGAGAA TGCAATAACA 
TCAAGCTCTG AAACCACCAC AATGGCTGGC CAATATGGTG AAAGTGGAAG CACAACAATA 
ATGGATGAAC AAGAAACTGG TACGTCCAGC CAGTATATTA GTGTGACGAC GACAACGCAA 
ACTTCTGACA CGATGTCATC AGTAAAAAAG TCTACGGAAA TAGCGACACC AAGTAGTAGC 
ATTGTACCCA CCCCTTTGCA GTCCTATAGC GATGAATCAC AAATATCACA AACGCTTAGT 
CACAATCCGA AGTCCGTGGC GGAGTCAGAT AGCGATACAA CCTCCTCGGA ATCCTCAAGT 
AGCGTAATAA TATCCACATC CGATAGTTCA GCGGTACCGA GGGAAATAAG TCCAATAATA 
ACCACAGATT CTCAAATTTC CAAAGAAGAA GGCACATTGG CTCAAACTTC CAGTATCAGT 
GAAACAACGA GAATTGCACA AATGGTAACC AGAGTTTCAC AAATCTCCAG CATAACGGCA 
GCTTCAACAA TAGATGGCTT TAGTAGTGAA TCAACACAAA CAGATTTTTC CAACACTGTA 
TCGTTTGAAA ATTCAGTCGA AGAAGAATAT GCCATGAGCA AATCCCAGCT CTCTGAAAGT 
TACAGTTCAA GTTCAACCGT CTATTCTGGA GGGGAGTCGA CTGCAGACAA GACATCTAGC 
TCCCCCATTA CCAGCTTTTC ATCTTCTTAT AGCCAAACAA CGTCAACTGA AACCTCTGAG 
AGCAGCCGAG TGGCTGTTGG AGTATCCCGG CCCTCTAGCA TAACGCAAAC TACATCAATT 
GATAGTTTTA GTATGAGTGA AGTAGAGCTT TCCACATATT ATGACTTAAG TGCTGGCAAC 
TATCCTGACC AAGAACTGAT TGTCGATCGC CCGGCCACTT CTTCCACTGC CGAAACATCT 
TCAGAGGCAA GTCAAGGTGT AAGCCGTGAA TCTAACACTT TCGCAGTATC TTCGATTTCC 
ACGACAAACT TTATAGTTAG CAGTGCTTCG GATACTGTTG TTTCAACTTC AAGCACCAAT 
ACAGTGCCAT ACAGCTCTGT CCATTCAACC TTTGTTCATG CGACTTCCTC GTCGACTTAT 
ATTTCTTCTT CCCTTTATTC TTCCCCATCC TTATCTGCAT CCGTTTCCTC TCACTTTGGC 
GTTGCTCCTT TTCCATCTGC TTACATATCT TTCTCATCAG TACCCGTTGC AGTATCCTCG 
ACGTACACGT CTTCACCATC GGCTTCTGTT GTAGTACCTT CTGCCTATGC GTCTTCACCA 
TCAGTACCCG TTGCAGTATC CTCGACGTAC ACGTCTTCAC CATCAGCACC TGCTGCAATA 
TCCTCAACGT ACACGTCTTC ACCATCGGCA CCTGTTGCAG TATCCTCGAC GTACACGTCT 
TCACCATCGG CACCTGCTGC AATATCCTCA ACGTACACGT CTTCACCATC GGCACCTGTT 
GCAGTATCCT CGACGTACAC GTCTTCACCA TCAGCACCTG CTGCAATATC CTCAACGTAC 
ACGTCTTCAC CATCGGCACC TGTTGCAGTA TCCTCGACGT ACACGTCTTC ACCATCAGCA 
CCTGTTGCAA TATCCTCAAC GTACACGTCT TCACCATCGG TACCTGTTGC AGTATCCTCG 
ACGTACACGT CTTCACCATC AGCACCTGCT GCAATATCCT CAACGTACAC GTCTTCACCA 
TCGGCACCCG TTGCAGTATC CTCGACGTAC ACGTCTTCAC CATCAGCACC TGCTGCAATA 
TCCTCAACGT ACACGTCTTC ACCATCAGTA CCCGTTGCAG TATCCTCGAC GTACACGTCT 
TCACCATCAG CACCTGCTGC AATATCCTCA ACGTACACGT CTTCACCATC AGTACCCGTT 
GCAGTATCCT CGACGTACAC GTCTTCACCA TCAGCACCTG CTGCAATATC CTCAACGTAC 
ACGTCTTCAC CATCGGCACC CGTTGCAGTA TCCTCGACGT ACACGTCTTC ACCATCAGCA 
CCTGCTGCAA TATCCTCAAC GTACACGTCT TCACCATCGG CACCCGTTGC AGTATCCTCG 
ACGTACACGT CTTCACCATC AGCACCTGCT GCAATATCCT CAACGTACAC GTCTTCACCA 
TCGGCACCTG TTGCAGTATC CTCGACGTAC ACGTCTTCAC CATCGGCTCT TGTTGTCCTG 
TCATCTACTT CCACATCCTC TCCATATGAT ATTGTTTACT CTCCATCAAC TTTTGCTGCC 
ATATCATCCG GTTACACGCC TTCACCTTCT GCTTCTGTCG CCATGTCATC TACTTCCTCA 
TCCTCTCCAT ATGATATTGT TTACTCTCTA TCATCTTCTG CTTCCAGATC GTCCATTGCT 
ACATATGAAT TTTCACCTTC CCCATCTACT TCTTTACCCA CCTCTTCTAC TTACACATAC 
TTTTCATCTG CTTATGCGTT TGAATTTTCT TCTGAGCGGT ACTCAACAAC ATCCACAATA 
GCACCAACTC AAATACATAG CACATTGTCC AGGATTACGG ATTTCTTACT GCAGACTTCG 
ATGGCAATCC AAAGTATAGT CTCTCAGCAA ATTTCTACAA GCTCAACTTT AAATGACGAA 
ATACATTCAA GCGCGCTTTC CGTTTTCAAC CCTTCAGCTT CGAATTTGGT TGAAACTTCT 
CTGATAATTT CATCGACCCA AGCAAGCATA ACAAGCCCCA AAAACTCAGC TAAAATTAGT 
TCACTACAGA GCCAGCTGTC TAGCAGTACA AAAAATCCAT ACGATACGGC GAACAAGAAT 
ACTGAGACCA GTGGCAGATC CACAGTTGTT TCGAATTTCC TATACACATC TTCCGCGGCG 
AAACCAGACA ATGAAAAGTT TTCCGCTACT CCTACAGAGA TAACTACAAT TTCAAGTAGC 
TCGCACGCAT ATTCTTTATC TATTCCAAGT TCTCATAATT CTGTGACTGG ACTATCACAT 
AACTTTGTCG ATTCGTCAAA AAGTGCAACC TCATTTGGCT ACTCATCCTC ATCAATATCT 
TCAATAAAAC TTTCAAAAGA AACAATACCT GCAAGTAAAT CTGTTTCAAA CACACAAGAA 
AGAATCACAA GCTTTACATC AACATTACGA GCAAATAGTC AGTCTGAAAA ATCCGAAGGG 
AGGAATTCTG TAGGTTCTTT ACAAAGTTCA CATATTTCAT CTAACCCATC GCTCTCCACA 
AATACAAAGG TTGATTCGAA AAGTTTATCC AGGAAAGTAT CGAAAACCAT GGGCGAAAAT 
GGCGAGGAAA CCGGCTTAAC CACAACAAAG ACGCAATACA AAAGTTCTTC TGAAACCAGT 
GGGTCATATT CTCGGTCTTT TACAAAAATC TCAATTGGAC CTGCTACGAC TGCAGTTCAA 
ACTCAGGCAA GCACTAATTC AGTATTCACA GCCCCTGCCT TGTCTACCTA TCCTACTACT 
CCGTACCCCT CTCCAAATAG TTACGCATGG TTACCTACCG CTATTATAGT GGAATCATCC 
GAAACGGGCC CCACTACCGC ATCATTCAAT CCGTCAATCA CTGGTTCACT TCCCAATGCA 
ATTGAACCGG CCGTCGCTGT ATCGGAACCC ATTAACCACA CTTTAATTAC CATCGGATTC 
ACGGCCGCTT TGAATTATGT ATTTTTGGTT CAAAATCCAT TATCTTCGGC TCAAATCTTC 
AACTTTTTAC CTCTCGTTTT GAAGTATCCC TTTTCAAACA CCTCATCAGA ACTGGACAAT 
AGTATTGGGG AATTATCCAC TTTTATTCTT TCATACCGGT CTGGGAGCAG CACAACTACC 
CTTTCCCCAA AATCCATATC ATCATTATCA GTTGTCAAGA AAAAAAAAAA CCAGCAGAAA 
AAAAATGCCA CGAAGTCAAC CGAGGACTTA CATCCTCCAC AAGTCGACAC ATCCTCAATA 
GCGGTGAAAA AGATTGTTCC CATGGTAGAC TCTTCTAAGG CATACATTGT ATCAGTTGCA 
GAGGTATATT TTCCAACAGA AGCCGTAACA TATCTTCAGC AATTAATCCT TGATGAAAAT 
TCGACTCTTT ATAGCAATCC TCAAACCCCA CTGCGAAGTT TAGCTGGTCT AATAGATAGT 
GGGATCCCAT TAGGGGGATT GACGTTATAT GGCTCTGGAG ATGGTGGTTA TGTCCCTAGT 
TTAACCTCAT CAAGCGTCTT GGATTCTTCA AAAGGAAATT CCCAAAACAT AGATGGAACT 
TACAAGTATG GAGCTTTGGA TGACTTTATC AATTCATTCA CAGATTCTGC ATCTGCAGGA 
AAGTATGCTG TAAAAATAAT CATCTTTTTG ATTGTTTTAA CTATTGGCGT ACTCTTGTGG 
TTATTCGTTG CATTTTTTGC TTTCCGTCAT AGAAATATAT TATTGAAAAG ACACCCAAGG 
AACTGCATAG GGAAAAGCCT CAATAATGAG AGGGAACTTG AAAGTACCGA GCTTTCCCGC 
TCTTCTTCTG GAAACCAAGT TTATAATGAA AAGCCGCCAG AATCTGAAAA TGAAAGTGTT 
TACTCTGCGG TAGATGATCA TTATATTGTG ACTGGCGAAA ACACAGTCTA TAATACTATT 
CACAGATTAC ATTATACCAT TAATGATGAC GGTGATCTAT TGTACAGGGA CGCAATCCCC 
CTCGATTTTG ATCAAACCAA TGGGGATGAT GGTTCGGGAA TAGACAGTAT AGTAAGAGAC 
TGCGTGTATG ACAAAAACCA AGATGCTACG GAAGCCTTCT TGAATGATGA GGAGTCCATA 
TCAGGCATTC TAGATGTTGA TGAAAATGGT GATATTCGAT TATACGACAG TTATTCTGAC 
AATGAAGAGT CAAACAGTTT TCACTTGCCA GACGAAGTTA TTGAAAACTA TAACAAGAAC 
CATTTGTGCG AAACTAAACT TCATGGACTA GGCACTGAAT CATGTACTAC AGATGATCCC 
GATACTGGAA ATCAAATTAC GAATGAGTTT TCTACAGGGA GTCAGACATG TTTGCCAAGT 
ACCGCTTATA CCACACCCCT TCATACAAAT TCGATTAAAC TTCACACATT ACGCTACACA 
GAATCTTCAC TACCAAAGCC AAATCAGACT CTTTTCTCTA ATCTTGAAGA TTTAGAAATA 
GAGGATATTG ACGACAATGG TAGTGTTTCA GATGTACATA TCGAGGAGCT GGATGCTTTA 
GACGAAGAAC TTTATAAGAG AATGTCGAAA GTTATAAAAC AACAGAACCA TCAAACGACA 
AAAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HKR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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