SUM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SUM1
Entrez Gene ID	851905
Full Name	Sum1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180618.1	NP_010596.1	Sum1p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AAL045C	NP_982497.1
Saccharomyces cerevisiae (baker's yeast)	SUM1	NP_010596.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

high copies of Sum1p stabilize microtubules against a variety of adverse and destabilizing conditions like mutations, low temperatures and drugs.
Title: High copies of SUM1 enhance the stability of wild-type microtubules against adverse conditions in Saccharomyces cerevisiae.

These observations suggest that Sum1p is a novel regulator of microtubule function and as such, works independently from its binding partners Hst1 and Sir2 histone deacetylases.
Title: The budding yeast protein Sum1 functions independently of its binding partners Hst1 and Sir2 histone deacetylases to regulate microtubule assembly.

Midsporulation elements in the SPS18-SPS19 promoter region are targeted by the Sum1p repressor in association with Hst1p and Rfm1p.
Title: Avoiding unscheduled transcription in shared promoters: Saccharomyces cerevisiae Sum1p represses the divergent gene pair SPS18-SPS19 through a midsporulation element (MSE).

Data show that the Sum1 repressor can be removed from middle promoters in meiotic cells independent of Ndt80 expression, and that this process requires the phosphorylation of Sum1 by Ime2.
Title: The Ime2 protein kinase enhances the disassociation of the Sum1 repressor from middle meiotic promoters.

Sum1 was found to functioned in initiation as a component of the Sum1/Rfm1/Hst1 complex, implying a role for histone deacetylation in origin activity.
Title: Control of replication initiation by the Sum1/Rfm1/Hst1 histone deacetylase.

The following SUM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SUM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01387	NM_001180618.1 Latest version!	Saccharomyces cerevisiae S288C Sum1p (SUM1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SUM1 ( NM_001180618.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010596.1 Saccharomyces cerevisiae S288C Sum1p (SUM1), partial mRNA.

Price & Availability↑

CloneID	OSi01387
Clone ID Related Accession (Same CDS sequence)	NM_001180618.1
Accession Version	NM_001180618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Sum1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGAGA ACACCACAGC CCCTTCTGAT AACATAACCA ATGAACAGAG ACTTCCGTCT 
GGACCCAAAG ATGACGTAGA TACGTTAGCA CTTACCAGTG CTCAAAATCA AGCTAATAGT 
TTGAGGAAGC TAGATACAGA TGCGAACGCT AAAGCTTTGC CATCCATCAC AGACATACCA 
GTCAGTGATG ATTCTGATAT AAAACGACAA GTTGGCTCTG GTTTTGGTTC AAATCCGCTT 
CATATAAAAG ATTCCGAGGC CTTTCCACAT TCATCTATTG AAGCCCTAAA AGAGGGTATG 
GACAAAGTCA CAAAACAATG CAACGATTTG AAGACGGCTT TGCTTTCCAA AGACACTTCC 
CTCACTGATT CTGTGCAGGA TTTGTTTAAC TCATTAAAAG TTTTATCACA CAACCAATCA 
GTTTTGGAAA ATAAGCTGGA TGATGTCATG AAGAATCAAG TAAATACTGA CATATTGGTC 
AATAATTTGA ATGAACGATT GAACAAATTA TCGACAATGT TGCAAAATAC TTCGAAAGTA 
AATCACTCTA ACCTTCTCAT AGAAAATTCA TCCAACAATA CCAGTTCACA GCATAATACC 
TCTTCTTCTC GAAGGGGACC TGGCAGGCCA AGAAAAGATG CCTCGACCTC CACAATGAAT 
AAGTTAGTGT CAAACGCAGC CTCGGTCAAT CTCAAAAGTG CATCCAATCA GGGTGCTCCT 
TTCTCACCAG TAAATATTAC TTTACCGACT GCCGTAGTAC AAACGTCTAA ATCCAAAAGG 
TATTTTGTGG AACCATCAAC GAAACAAGAG TCGCTTTTAT TATCTGCCCC TTCATCATCA 
AGAGATGATG CTGATATGTC CTTGACTTCC GTACCGCAAA GAACGAACAA TGAAAATGGT 
AAAGAGCGAC CATCCACTGC TAATTCTAGC TCCATCACAC CAACACCTGT TACGCCGAAC 
AACTTAATTC AAATCAAAAG AAAAAGAGGC AGGCCCCCAA AAAAGAGAAC AGTGGAAACA 
ATGATATCCA ATTCCACGGA TACGATAGAT AAGTCAGATG CATCTAATCG GATTAAAAAC 
GAGATTCCAA TAAATTCCTT GCTTCCATCT TCCAAATTTC ATCAAATACC ATCATCTCCA 
TCCAATCCTG TGTCACAACC GGCTCCGGTT CGGACTTCAA GGTCAGCCAC ACAAGAAATA 
GACATTAAAA GTTTAGAACT GGCCTCTCTA ATTTCAACTA ATGGTGATCC AAACGCAGAG 
GATTCGAATA CTACTGATAC TGTTCATAAT AACGTGGAAG GAAAGGTAAA TGTTGAAGAA 
AATAAAACCG AGAAGGAGAA AATAATAACC ATCAAATCAT CCAGCGAAAA TAGTGGTAAT 
AATACGACCA ATAATAATAA TACTGACAAC GTCATTAAAT TTTCAGCTAA TTCAGATATC 
AATAGTGATA TTCGCCGATT AATGGTTAAC GATCAGTTTT CATTAAGTTA TGACGCCAGC 
GGTAATATTA CGGTCAAATT ACCACCCGTT TCCTCTCCAG CAGCAGCAAC AGCTGCCGCT 
GCTGCGGCGG TTACGTCAGA GATGAATAGA CAACAAAGAG AATTAGATAA AAGACGTGAT 
TCAAGGGAGA AAATGCTTGT TAATATGAAA TATAACGATC GCGATAAAGC AAAGTCATTT 
ATGGAGTCTA ATAAGAAACT CTTAAAAGCA ATGAAAGAAG AAGAGAGGAG GAAAAGAATG 
ACTTCGATAA TACATGATAA TCACTTGAAC TTAAATTTGA ATGAGATCTC CACCCGTTCA 
AAGATAAAAA GTGCAGAAAA ACCAACAACT AAAGGTTCTT CAATGTCGCC AAAACCAAGA 
TCGGCCTCCA TCAGCGGCAT TTCAGACCAC CAACAGGAAG GATATCAACC ATTAGAGCAA 
GAAAAACTCG TCGATATTGA CAATGAGGGC TCAAACGCAA ACAGCGACTC TCTCAAGATG 
GGTCTAACCA TATCCGCTGC CGATACGGTT CACAAAGTTG GAATACAGTC CATGCTAAAT 
TCTGGGGAAG AGGCAATTAC CAAGGAGAAT GCAGAATATG AAAGAAAGAC CCCGGGAGAT 
GAAGAAACTA CCACGTTCGT TCCATTAGAA AACTCACAAC CTTCGGACAC GATAAGGAAA 
AGAACAGCAG GCGATGACGG TGCATTGGAT CAAACAGAGA ATACGAGCAT ATCACCAAAG 
AAGAGACGCA CAGAAGATCA TACAAAAGGT GAAGAAGATG AGGGAGAAAG GGGTGTTGGT 
AATAGCGGAA CGCTGGCCAC CGTGGAGAAT GTCTCAGGAG ACATCTCCGC AGATTTATCC 
AAAGGTACTT CATCTATCCA TAACGATACT GAGTCTGCTA ACGATAGTAG CAATGGCAAC 
GGAAACCTCG GTTTGGGTAC AGAGTCACGC AATACATTGT TAACGGCAAC TCCCATTGAA 
TTAATCTGCC GGGAAGGCTT CTTCTACCGG AGAGATATTC CGGACGTTCC CATTACCACA 
GGAGCGTACC TGGAATTTAA ATTCAAGGCG AAAGAAGAGG AATTGATCAA CTCTAGCATC 
AATGAGGAGG ATTATGCTGC TAAATCAAAG CATGAAAAAA TGAATGCGCA TTTCTTCAAG 
CCTGATATTC AAGAAGAGAC GGAACTTGCC TTTGAAATTT TGAGCAAGAC AACGCTGACA 
GAGAAATACG TTAACAGTTT GGAGTACTTC TTGATGGAAT TCAGGTGGGA GAATAAACTA 
GTTGGTCTAG GCTTAAAACT TCGGGAATCC AAAAGAACCT GGCAAAGGAG AAAGGCGTTA 
TTTGCCCTTT TCGAATTTTG GAGAGACCAA TCAAGAGACA AAAGAAGATT CCACAACTAC 
ACCATCCTGC ATGCGGTGAA AGAGATGGAA AACTACAGAA TATTTATTAA TCGATCCGTT 
TCATGGTTCT ACAACCATAT TACCCTGCTA AAAATGATCC TCTACGACCT CTGCGACAAT 
GTGACCACTC AATGGAGAGA GTGGATGTTT CCCCATAACG AAACACTGCC GGCATTGGGT 
CAGGACGGCA TTAACGAAGA CAATCTGAAT GAAACCATCG ACAACATGTT AATTTTTGAC 
TTCCTTGATG ACGGTTCAGA AAACAACCAG GTCAAATATT CCAGAATCAT ACCGCCAGAT 
ATCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010596.1
CDS	1..3189
Translation	MSENTTAPSDNITNEQRLPSGPKDDVDTLALTSAQNQANSLRKLDTDANAKALPSITDIPVSDDSDIKRQVGSGFGSNPLHIKDSEAFPHSSIEALKEGMDKVTKQCNDLKTALLSKDTSLTDSVQDLFNSLKVLSHNQSVLENKLDDVMKNQVNTDILVNNLNERLNKLSTMLQNTSKVNHSNLLIENSSNNTSSQHNTSSSRRGPGRPRKDASTSTMNKLVSNAASVNLKSASNQGAPFSPVNITLPTAVVQTSKSKRYFVEPSTKQESLLLSAPSSSRDDADMSLTSVPQRTNNENGKERPSTANSSSITPTPVTPNNLIQIKRKRGRPPKKRTVETMISNSTDTIDKSDASNRIKNEIPINSLLPSSKFHQIPSSPSNPVSQPAPVRTSRSATQEIDIKSLELASLISTNGDPNAEDSNTTDTVHNNVEGKVNVEENKTEKEKIITIKSSSENSGNNTTNNNNTDNVIKFSANSDINSDIRRLMVNDQFSLSYDASGNITVKLPPVSSPAAATAAAAAAVTSEMNRQQRELDKRRDSREKMLVNMKYNDRDKAKSFMESNKKLLKAMKEEERRKRMTSIIHDNHLNLNLNEISTRSKIKSAEKPTTKGSSMSPKPRSASISGISDHQQEGYQPLEQEKLVDIDNEGSNANSDSLKMGLTISAADTVHKVGIQSMLNSGEEAITKENAEYERKTPGDEETTTFVPLENSQPSDTIRKRTAGDDGALDQTENTSISPKKRRTEDHTKGEEDEGERGVGNSGTLATVENVSGDISADLSKGTSSIHNDTESANDSSNGNGNLGLGTESRNTLLTATPIELICREGFFYRRDIPDVPITTGAYLEFKFKAKEEELINSSINEEDYAAKSKHEKMNAHFFKPDIQEETELAFEILSKTTLTEKYVNSLEYFLMEFRWENKLVGLGLKLRESKRTWQRRKALFALFEFWRDQSRDKRRFHNYTILHAVKEMENYRIFINRSVSWFYNHITLLKMILYDLCDNVTTQWREWMFPHNETLPALGQDGINEDNLNETIDNMLIFDFLDDGSENNQVKYSRIIPPDIR

Target ORF information:

RefSeq Version	NM_001180618.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Sum1p (SUM1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180618.1

ORF Insert Sequence:

ATGTCTGAGA ACACCACAGC CCCTTCTGAT AACATAACCA ATGAACAGAG ACTTCCGTCT 
GGACCCAAAG ATGACGTAGA TACGTTAGCA CTTACCAGTG CTCAAAATCA AGCTAATAGT 
TTGAGGAAGC TAGATACAGA TGCGAACGCT AAAGCTTTGC CATCCATCAC AGACATACCA 
GTCAGTGATG ATTCTGATAT AAAACGACAA GTTGGCTCTG GTTTTGGTTC AAATCCGCTT 
CATATAAAAG ATTCCGAGGC CTTTCCACAT TCATCTATTG AAGCCCTAAA AGAGGGTATG 
GACAAAGTCA CAAAACAATG CAACGATTTG AAGACGGCTT TGCTTTCCAA AGACACTTCC 
CTCACTGATT CTGTGCAGGA TTTGTTTAAC TCATTAAAAG TTTTATCACA CAACCAATCA 
GTTTTGGAAA ATAAGCTGGA TGATGTCATG AAGAATCAAG TAAATACTGA CATATTGGTC 
AATAATTTGA ATGAACGATT GAACAAATTA TCGACAATGT TGCAAAATAC TTCGAAAGTA 
AATCACTCTA ACCTTCTCAT AGAAAATTCA TCCAACAATA CCAGTTCACA GCATAATACC 
TCTTCTTCTC GAAGGGGACC TGGCAGGCCA AGAAAAGATG CCTCGACCTC CACAATGAAT 
AAGTTAGTGT CAAACGCAGC CTCGGTCAAT CTCAAAAGTG CATCCAATCA GGGTGCTCCT 
TTCTCACCAG TAAATATTAC TTTACCGACT GCCGTAGTAC AAACGTCTAA ATCCAAAAGG 
TATTTTGTGG AACCATCAAC GAAACAAGAG TCGCTTTTAT TATCTGCCCC TTCATCATCA 
AGAGATGATG CTGATATGTC CTTGACTTCC GTACCGCAAA GAACGAACAA TGAAAATGGT 
AAAGAGCGAC CATCCACTGC TAATTCTAGC TCCATCACAC CAACACCTGT TACGCCGAAC 
AACTTAATTC AAATCAAAAG AAAAAGAGGC AGGCCCCCAA AAAAGAGAAC AGTGGAAACA 
ATGATATCCA ATTCCACGGA TACGATAGAT AAGTCAGATG CATCTAATCG GATTAAAAAC 
GAGATTCCAA TAAATTCCTT GCTTCCATCT TCCAAATTTC ATCAAATACC ATCATCTCCA 
TCCAATCCTG TGTCACAACC GGCTCCGGTT CGGACTTCAA GGTCAGCCAC ACAAGAAATA 
GACATTAAAA GTTTAGAACT GGCCTCTCTA ATTTCAACTA ATGGTGATCC AAACGCAGAG 
GATTCGAATA CTACTGATAC TGTTCATAAT AACGTGGAAG GAAAGGTAAA TGTTGAAGAA 
AATAAAACCG AGAAGGAGAA AATAATAACC ATCAAATCAT CCAGCGAAAA TAGTGGTAAT 
AATACGACCA ATAATAATAA TACTGACAAC GTCATTAAAT TTTCAGCTAA TTCAGATATC 
AATAGTGATA TTCGCCGATT AATGGTTAAC GATCAGTTTT CATTAAGTTA TGACGCCAGC 
GGTAATATTA CGGTCAAATT ACCACCCGTT TCCTCTCCAG CAGCAGCAAC AGCTGCCGCT 
GCTGCGGCGG TTACGTCAGA GATGAATAGA CAACAAAGAG AATTAGATAA AAGACGTGAT 
TCAAGGGAGA AAATGCTTGT TAATATGAAA TATAACGATC GCGATAAAGC AAAGTCATTT 
ATGGAGTCTA ATAAGAAACT CTTAAAAGCA ATGAAAGAAG AAGAGAGGAG GAAAAGAATG 
ACTTCGATAA TACATGATAA TCACTTGAAC TTAAATTTGA ATGAGATCTC CACCCGTTCA 
AAGATAAAAA GTGCAGAAAA ACCAACAACT AAAGGTTCTT CAATGTCGCC AAAACCAAGA 
TCGGCCTCCA TCAGCGGCAT TTCAGACCAC CAACAGGAAG GATATCAACC ATTAGAGCAA 
GAAAAACTCG TCGATATTGA CAATGAGGGC TCAAACGCAA ACAGCGACTC TCTCAAGATG 
GGTCTAACCA TATCCGCTGC CGATACGGTT CACAAAGTTG GAATACAGTC CATGCTAAAT 
TCTGGGGAAG AGGCAATTAC CAAGGAGAAT GCAGAATATG AAAGAAAGAC CCCGGGAGAT 
GAAGAAACTA CCACGTTCGT TCCATTAGAA AACTCACAAC CTTCGGACAC GATAAGGAAA 
AGAACAGCAG GCGATGACGG TGCATTGGAT CAAACAGAGA ATACGAGCAT ATCACCAAAG 
AAGAGACGCA CAGAAGATCA TACAAAAGGT GAAGAAGATG AGGGAGAAAG GGGTGTTGGT 
AATAGCGGAA CGCTGGCCAC CGTGGAGAAT GTCTCAGGAG ACATCTCCGC AGATTTATCC 
AAAGGTACTT CATCTATCCA TAACGATACT GAGTCTGCTA ACGATAGTAG CAATGGCAAC 
GGAAACCTCG GTTTGGGTAC AGAGTCACGC AATACATTGT TAACGGCAAC TCCCATTGAA 
TTAATCTGCC GGGAAGGCTT CTTCTACCGG AGAGATATTC CGGACGTTCC CATTACCACA 
GGAGCGTACC TGGAATTTAA ATTCAAGGCG AAAGAAGAGG AATTGATCAA CTCTAGCATC 
AATGAGGAGG ATTATGCTGC TAAATCAAAG CATGAAAAAA TGAATGCGCA TTTCTTCAAG 
CCTGATATTC AAGAAGAGAC GGAACTTGCC TTTGAAATTT TGAGCAAGAC AACGCTGACA 
GAGAAATACG TTAACAGTTT GGAGTACTTC TTGATGGAAT TCAGGTGGGA GAATAAACTA 
GTTGGTCTAG GCTTAAAACT TCGGGAATCC AAAAGAACCT GGCAAAGGAG AAAGGCGTTA 
TTTGCCCTTT TCGAATTTTG GAGAGACCAA TCAAGAGACA AAAGAAGATT CCACAACTAC 
ACCATCCTGC ATGCGGTGAA AGAGATGGAA AACTACAGAA TATTTATTAA TCGATCCGTT 
TCATGGTTCT ACAACCATAT TACCCTGCTA AAAATGATCC TCTACGACCT CTGCGACAAT 
GTGACCACTC AATGGAGAGA GTGGATGTTT CCCCATAACG AAACACTGCC GGCATTGGGT 
CAGGACGGCA TTAACGAAGA CAATCTGAAT GAAACCATCG ACAACATGTT AATTTTTGAC 
TTCCTTGATG ACGGTTCAGA AAACAACCAG GTCAAATATT CCAGAATCAT ACCGCCAGAT 
ATCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SUM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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