ORF » Species Summary » Saccharomyces cerevisiae S288C » HEL2 cDNA ORF clone

HEL2 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following HEL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01348 NM_001180574.3
Latest version!		 Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase HEL2 (HEL2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    HEL2 ( NM_001180574.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010552.3 Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase HEL2 (HEL2), partial mRNA.
    Price & Availability↑
    CloneID OSi01348
    Clone ID Related Accession (Same CDS sequence) NM_001180574.3
    Accession Version NM_001180574.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product E3 ubiquitin-protein ligase HEL2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180574.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGCGAAT CAGTGAAAGA AAACGTTACT CCGACACGTA ACTTCCGTCG TACTCAAGGG 
    CCCCAAAATA ATACAAAACC TCACAACGAC AGGAAGAATT TCAGAAGAAA GCAAAAGAAA 
    AATAACCTTT CTGCTGAACC CAACCTCACC ACTTCAAGCG CAGATGATAC TGATGAAGAA 
    AATGAATTAT GTGTAATTTG TGCGCGCAAG TTAACATACG TATCTCTAAC ACCATGCCAT 
    CATAAGACAT GCCATATCTG TGGTTTCAGA CAACGAGCTT TGTACAACAA GAAAAGCTGT 
    TTGATTTGCA GAACGGAGAA TGAAGAAGTC ATGTTTACCG ATCGCATAGA TGGTGATATT 
    TCAGATAAAT ATAACTTTTG CGAAAAAAAT GAAAAATATG GAATAAATTT CACTAGCGAA 
    GAGGTTGCTA CCGAAACACT AAATCTTTTA AAATTTTTTT GTCCCCTATC GAAGGATGAA 
    CAAGTTTGTG ACTTTGGTAG TTTCAAGAAA TATAATGAAC ACTTGAAATC TGAACATAAC 
    AGAATGATCT GTTTAATATG TGCAACCCAT AAGCATGCAT TCCCTTGTGA GCTTGAAATA 
    TTCACCCAAA ATCAATTACG CAACCATCAA ACTAAGGGTA ATTCAGAAGG ATTCAAAGGC 
    CACCCTATGT GTGCCTTCTG TTCCGGAAAA CGATTTTATT CAGACGACGA ATTGTACATT 
    CACATGAGAA ATCAGCATGA AAAGTGCCAT ATTTGTGATA AGATGAACCC CGCTTCTCCT 
    CAATACTTTA AGGATTATAA TCAGCTTTTT GACCACTTTA AACATTCGCA TTACGTTTGC 
    ACCGTCCAAA CTTGCTTAGA TAACAAGTTT GTCGTATTTA AAGACGAGTT GGAGTTACAA 
    GCCCACATTT TGCAGGAGCA TGGTAATATC CTGAAGGGAA AGCCAAAGTT TTTCCAGTCA 
    GAACTTTCTA CTTTTATCTC GGCTCCATCT AGAGTGATCA GGGAAAGAGA TGATTACGAC 
    CTTCCATCTA TATCTTCCCT ACCGGGTTCA TCATCCGGCT CTAGAACTGA TGTGAGATCT 
    GCTAGTTCTC CCGAGGAAAG TAGACTAAGA TTGGCAGAAA GAGCAAAATA CTATCTGGAA 
    AATTCCAAAG AAGACTTCAA TAAGTTTTCT TCTTATAACG AAGATTATTC TAAAGGTCGC 
    TTAAGTGCTG AAAAATTATT GGAATCATAT AAATTGTTAT TCACAAAACC AAATGCGGAT 
    GTATATTTAT TGATTCATAA CTTAGCTGAA ACCTTTCCCA AAAACTCCTC CAAATACAAT 
    AATCTGAACG CCATATATGA ACAAAGAGAG CAAACCCTTG CACGACAAAC TTCACTACCT 
    TCTTTATCTA GTGATTCTTC CCTTTCAATG TCAATAGGCA GGGGCCATTG GGGTGGAACA 
    AACGATGGGG GCAGCGCTGG TGCAGCATTA GGCGTTAGGA ATATCAAAAA TCTTCCAACC 
    CTGAAAAGTC CTTCAGCGTC GTATGATCCC TTTGCAACCA CCGTAAAAAA AAACACTCTG 
    AGACCCGTGC AAAACATTAA AAGAACTACA CCTCAATCAG TTAGTTATCG AACTTCCACT 
    AATACAGTCG CCTTCTCTCC AACCTATTTG GAAAGTAAAA AGGGTTCCTC TTCTACTTCT 
    CTAAATAATA GCAAAGATAA ATTGAAAAGT CTCAACCTAC CTCAACTACC TCCACCAAAG 
    CCAAAGGTGC AAATTCCAGG TCTGAACAGA CCCCAAATTG CTGATCCTAA GCAGTGGGGT 
    AAAAAGTCAA GCACACAAGA TACTAACGTG CACGATAATT TGAGGGAATT GAACACAACT 
    AGTGGAGGAA ATAAAAAGAA AGGCAAACAA AAGCAGCTGT TATTCCACAT TGGTGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010552.3
    CDS1..1920
    Translation

    Target ORF information:

    RefSeq Version NM_001180574.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C E3 ubiquitin-protein ligase HEL2 (HEL2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001180574.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAGCGAAT CAGTGAAAGA AAACGTTACT CCGACACGTA ACTTCCGTCG TACTCAAGGG 
    CCCCAAAATA ATACAAAACC TCACAACGAC AGGAAGAATT TCAGAAGAAA GCAAAAGAAA 
    AATAACCTTT CTGCTGAACC CAACCTCACC ACTTCAAGCG CAGATGATAC TGATGAAGAA 
    AATGAATTAT GTGTAATTTG TGCGCGCAAG TTAACATACG TATCTCTAAC ACCATGCCAT 
    CATAAGACAT GCCATATCTG TGGTTTCAGA CAACGAGCTT TGTACAACAA GAAAAGCTGT 
    TTGATTTGCA GAACGGAGAA TGAAGAAGTC ATGTTTACCG ATCGCATAGA TGGTGATATT 
    TCAGATAAAT ATAACTTTTG CGAAAAAAAT GAAAAATATG GAATAAATTT CACTAGCGAA 
    GAGGTTGCTA CCGAAACACT AAATCTTTTA AAATTTTTTT GTCCCCTATC GAAGGATGAA 
    CAAGTTTGTG ACTTTGGTAG TTTCAAGAAA TATAATGAAC ACTTGAAATC TGAACATAAC 
    AGAATGATCT GTTTAATATG TGCAACCCAT AAGCATGCAT TCCCTTGTGA GCTTGAAATA 
    TTCACCCAAA ATCAATTACG CAACCATCAA ACTAAGGGTA ATTCAGAAGG ATTCAAAGGC 
    CACCCTATGT GTGCCTTCTG TTCCGGAAAA CGATTTTATT CAGACGACGA ATTGTACATT 
    CACATGAGAA ATCAGCATGA AAAGTGCCAT ATTTGTGATA AGATGAACCC CGCTTCTCCT 
    CAATACTTTA AGGATTATAA TCAGCTTTTT GACCACTTTA AACATTCGCA TTACGTTTGC 
    ACCGTCCAAA CTTGCTTAGA TAACAAGTTT GTCGTATTTA AAGACGAGTT GGAGTTACAA 
    GCCCACATTT TGCAGGAGCA TGGTAATATC CTGAAGGGAA AGCCAAAGTT TTTCCAGTCA 
    GAACTTTCTA CTTTTATCTC GGCTCCATCT AGAGTGATCA GGGAAAGAGA TGATTACGAC 
    CTTCCATCTA TATCTTCCCT ACCGGGTTCA TCATCCGGCT CTAGAACTGA TGTGAGATCT 
    GCTAGTTCTC CCGAGGAAAG TAGACTAAGA TTGGCAGAAA GAGCAAAATA CTATCTGGAA 
    AATTCCAAAG AAGACTTCAA TAAGTTTTCT TCTTATAACG AAGATTATTC TAAAGGTCGC 
    TTAAGTGCTG AAAAATTATT GGAATCATAT AAATTGTTAT TCACAAAACC AAATGCGGAT 
    GTATATTTAT TGATTCATAA CTTAGCTGAA ACCTTTCCCA AAAACTCCTC CAAATACAAT 
    AATCTGAACG CCATATATGA ACAAAGAGAG CAAACCCTTG CACGACAAAC TTCACTACCT 
    TCTTTATCTA GTGATTCTTC CCTTTCAATG TCAATAGGCA GGGGCCATTG GGGTGGAACA 
    AACGATGGGG GCAGCGCTGG TGCAGCATTA GGCGTTAGGA ATATCAAAAA TCTTCCAACC 
    CTGAAAAGTC CTTCAGCGTC GTATGATCCC TTTGCAACCA CCGTAAAAAA AAACACTCTG 
    AGACCCGTGC AAAACATTAA AAGAACTACA CCTCAATCAG TTAGTTATCG AACTTCCACT 
    AATACAGTCG CCTTCTCTCC AACCTATTTG GAAAGTAAAA AGGGTTCCTC TTCTACTTCT 
    CTAAATAATA GCAAAGATAA ATTGAAAAGT CTCAACCTAC CTCAACTACC TCCACCAAAG 
    CCAAAGGTGC AAATTCCAGG TCTGAACAGA CCCCAAATTG CTGATCCTAA GCAGTGGGGT 
    AAAAAGTCAA GCACACAAGA TACTAACGTG CACGATAATT TGAGGGAATT GAACACAACT 
    AGTGGAGGAA ATAAAAAGAA AGGCAAACAA AAGCAGCTGT TATTCCACAT TGGTGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
    Nature387(6632 Suppl)75-8(1997 May)
    Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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