PCF11 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PCF11
Entrez Gene ID	851814
Full Name	Pcf11p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180536.3	NP_010514.3	Pcf11p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR190C	NP_986856.1
Saccharomyces cerevisiae (baker's yeast)	PCF11	NP_010514.3
Kluyveromyces lactis	KLLA0F13442g	XP_455685.1
Schizosaccharomyces pombe (fission yeast)	SPAC4G9.04c	NP_593686.1
Magnaporthe oryzae (rice blast fungus)	MGG_08573	XP_003716097.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study describes a novel flower-like structure for the C-terminal CCHC zinc-finger domain of ScPcf11 that is only distantly related to other zinc coordinating domains.
Title: The C terminus of Pcf11 forms a novel zinc-finger structure that plays an essential role in mRNA 3'-end processing.

Pcf11, a component of the cleavage and polyadenylation complex (CPAC), is also generally required for NRD-dependent transcription termination through the action of its C-terminal domain (CTD)-interacting domain
Title: Pcf11 orchestrates transcription termination pathways in yeast.

X-ray crystallographic evidence of an extended binding interface of Pcf11p with Clp1p which is likely to be S. cerevisiae specific.
Title: Structural basis for ATP loss by Clp1p in a G135R mutant protein.

Mutations in Pcf11 that perturb Clp1 binding cause temperature-sensitive growth and affect cleavage, polyadenylation and transcription termination.
Title: The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site.

CF IA is reconstituted from Rna14, Rna15, Pcf11, and Clp1 proteins in 2:2:1:1 stoichiometry.
Title: Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology.

The following PCF11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCF11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01309	NM_001180536.3 Latest version!	Saccharomyces cerevisiae S288C Pcf11p (PCF11), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCF11 ( NM_001180536.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010514.3 Saccharomyces cerevisiae S288C Pcf11p (PCF11), partial mRNA.

Price & Availability↑

CloneID	OSi01309
Clone ID Related Accession (Same CDS sequence)	NM_001180536.3
Accession Version	NM_001180536.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Pcf11p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180536.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATCACG ACACAGAAGT TATAGTCAAG GATTTCAACA GTATTCTTGA AGAATTAACT 
TTTAACTCGA GACCCATTAT TACCACTCTC ACGAAGTTAG CAGAAGAAAA CATTTCGTGT 
GCTCAATATT TTGTTGACGC CATTGAGAGT AGAATTGAAA AATGTATGCC TAAGCAGAAG 
CTATACGCTT TTTATGCCTT GGACTCCATT TGTAAGAATG TTGGCAGCCC ATACACAATC 
TACTTCAGTA GAAATTTGTT TAACTTGTAC AAAAGAACAT ACTTATTGGT GGACAACACC 
ACAAGAACAA AACTCATCAA TATGTTTAAG CTTTGGTTGA ATCCTAATGA CACCGGCCTG 
CCTCTGTTTG AAGGCTCAGC ACTGGAGAAA ATTGAACAGT TTTTAATAAA GGCCAGTGCT 
CTCCACCAGA AAAACCTGCA AGCAATGTTA CCAACTCCGA CTGTTCCCCT CCTATTAAGA 
GACATTGATA AATTAACTTG TTTAACTAGT GAAAGGCTAA AGAATCAACC AAATGATGAA 
AAATTAAAAA TGAAACTGTT GGTTTTATCT CAGTTGAAAC AAGAACTGAA AAGAGAAAAA 
CTAACTCTCA ATGCTCTGAA ACAAGTTCAA ATGCAACTAA GGCAGGTCTT CTCACAGGAC 
CAACAAGTTT TGCAGGAAAG AATGAGGTAT CACGAACTTC AGCAGCAACA GCAACAACAG 
CAGCAACAGC AACAACAACA ACAGCAGCAG CAGCAGCAGT ATCACGAAAC TAAGGATATG 
GTCGGTTCTT ACACACAAAA CTCTAATTCA GCGATTCCAT TGTTTGGAAA TAATTCAGAT 
ACAACGAACC AACAGAATTC TTTATCTTCC TCTCTTTTTG GTAATATTTC TGGCGTTGAA 
TCTTTTCAAG AAATTGAGAA AAAAAAGTCC CTAAACAAAA TCAATAACCT CTATGCATCT 
CTTAAGGCTG AAGGGCTCAT ATATACTCCA CCAAAAGAAT CCATCGTAAC GCTTTATAAA 
AAGCTAAATG GACACTCAAA CTACTCTCTT GATTCACACG AAAAACAATT GATGAAGAAC 
CTGCCAAAGA TACCCTTATT AAATGATATT CTTTCTGACT GCAAAGCTTA TTTTGCCACG 
GTTAACATCG ACGTTTTGAA TAACCCATCC CTGCAGCTGT CCGAACAAAC TCTTCTTCAA 
GAAAATCCCA TCGTCCAAAA TAATCTTATT CACTTATTAT ACCGATCCAA GCCAAATAAA 
TGTAGCGTGT GTGGTAAGAG ATTTGGCAAC TCAGAAAGTG AAAAACTACT ACAAAATGAG 
CATTTGGACT GGCATTTCAG AATAAATACA AGAATTAAGG GATCACAAAA CACAGCAAAT 
ACAGGCATAA GCAACTCTAA CTTAAATACA ACTACCACAA GGAAAAATAT TCAATCAAGA 
AACTGGTATT TGAGTGACTC TCAATGGGCA GCCTTCAAAG ATGATGAAAT TACCTCCACA 
AAGCATAAAA ATGATTATAC TGATCCGCAT GCCAATAAAA ATATCGATAA AAGTGCCCTA 
AATATTCACG CTGATGAAAA TGACGAAGGC TCTGTAGACA ACACTTTAGG AAGTGATAGA 
AGTAACGAGT TGGAAATACG GGGAAAATAT GTTGTTGTAC CAGAAACTTC ACAAGATATG 
GCCTTCAAAT GTCCAATTTG TAAGGAAACC GTTACTGGCG TTTATGATGA AGAATCTGGT 
GAATGGGTTT GGAAAAATAC TATTGAAGTG AATGGCAAAT ATTTCCACTC GACATGTTAT 
CATGAAACGT CGCAAAATTC AAGCAAATCT AATAGTGGCA AGGTCGGTTT GGATGACTTA 
AAGAAATTGG TCACAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010514.3
CDS	1..1881
Translation	MDHDTEVIVKDFNSILEELTFNSRPIITTLTKLAEENISCAQYFVDAIESRIEKCMPKQKLYAFYALDSICKNVGSPYTIYFSRNLFNLYKRTYLLVDNTTRTKLINMFKLWLNPNDTGLPLFEGSALEKIEQFLIKASALHQKNLQAMLPTPTVPLLLRDIDKLTCLTSERLKNQPNDEKLKMKLLVLSQLKQELKREKLTLNALKQVQMQLRQVFSQDQQVLQERMRYHELQQQQQQQQQQQQQQQQQQQQYHETKDMVGSYTQNSNSAIPLFGNNSDTTNQQNSLSSSLFGNISGVESFQEIEKKKSLNKINNLYASLKAEGLIYTPPKESIVTLYKKLNGHSNYSLDSHEKQLMKNLPKIPLLNDILSDCKAYFATVNIDVLNNPSLQLSEQTLLQENPIVQNNLIHLLYRSKPNKCSVCGKRFGNSESEKLLQNEHLDWHFRINTRIKGSQNTANTGISNSNLNTTTTRKNIQSRNWYLSDSQWAAFKDDEITSTKHKNDYTDPHANKNIDKSALNIHADENDEGSVDNTLGSDRSNELEIRGKYVVVPETSQDMAFKCPICKETVTGVYDEESGEWVWKNTIEVNGKYFHSTCYHETSQNSSKSNSGKVGLDDLKKLVTK

Target ORF information:

RefSeq Version	NM_001180536.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Pcf11p (PCF11), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180536.3

ORF Insert Sequence:

ATGGATCACG ACACAGAAGT TATAGTCAAG GATTTCAACA GTATTCTTGA AGAATTAACT 
TTTAACTCGA GACCCATTAT TACCACTCTC ACGAAGTTAG CAGAAGAAAA CATTTCGTGT 
GCTCAATATT TTGTTGACGC CATTGAGAGT AGAATTGAAA AATGTATGCC TAAGCAGAAG 
CTATACGCTT TTTATGCCTT GGACTCCATT TGTAAGAATG TTGGCAGCCC ATACACAATC 
TACTTCAGTA GAAATTTGTT TAACTTGTAC AAAAGAACAT ACTTATTGGT GGACAACACC 
ACAAGAACAA AACTCATCAA TATGTTTAAG CTTTGGTTGA ATCCTAATGA CACCGGCCTG 
CCTCTGTTTG AAGGCTCAGC ACTGGAGAAA ATTGAACAGT TTTTAATAAA GGCCAGTGCT 
CTCCACCAGA AAAACCTGCA AGCAATGTTA CCAACTCCGA CTGTTCCCCT CCTATTAAGA 
GACATTGATA AATTAACTTG TTTAACTAGT GAAAGGCTAA AGAATCAACC AAATGATGAA 
AAATTAAAAA TGAAACTGTT GGTTTTATCT CAGTTGAAAC AAGAACTGAA AAGAGAAAAA 
CTAACTCTCA ATGCTCTGAA ACAAGTTCAA ATGCAACTAA GGCAGGTCTT CTCACAGGAC 
CAACAAGTTT TGCAGGAAAG AATGAGGTAT CACGAACTTC AGCAGCAACA GCAACAACAG 
CAGCAACAGC AACAACAACA ACAGCAGCAG CAGCAGCAGT ATCACGAAAC TAAGGATATG 
GTCGGTTCTT ACACACAAAA CTCTAATTCA GCGATTCCAT TGTTTGGAAA TAATTCAGAT 
ACAACGAACC AACAGAATTC TTTATCTTCC TCTCTTTTTG GTAATATTTC TGGCGTTGAA 
TCTTTTCAAG AAATTGAGAA AAAAAAGTCC CTAAACAAAA TCAATAACCT CTATGCATCT 
CTTAAGGCTG AAGGGCTCAT ATATACTCCA CCAAAAGAAT CCATCGTAAC GCTTTATAAA 
AAGCTAAATG GACACTCAAA CTACTCTCTT GATTCACACG AAAAACAATT GATGAAGAAC 
CTGCCAAAGA TACCCTTATT AAATGATATT CTTTCTGACT GCAAAGCTTA TTTTGCCACG 
GTTAACATCG ACGTTTTGAA TAACCCATCC CTGCAGCTGT CCGAACAAAC TCTTCTTCAA 
GAAAATCCCA TCGTCCAAAA TAATCTTATT CACTTATTAT ACCGATCCAA GCCAAATAAA 
TGTAGCGTGT GTGGTAAGAG ATTTGGCAAC TCAGAAAGTG AAAAACTACT ACAAAATGAG 
CATTTGGACT GGCATTTCAG AATAAATACA AGAATTAAGG GATCACAAAA CACAGCAAAT 
ACAGGCATAA GCAACTCTAA CTTAAATACA ACTACCACAA GGAAAAATAT TCAATCAAGA 
AACTGGTATT TGAGTGACTC TCAATGGGCA GCCTTCAAAG ATGATGAAAT TACCTCCACA 
AAGCATAAAA ATGATTATAC TGATCCGCAT GCCAATAAAA ATATCGATAA AAGTGCCCTA 
AATATTCACG CTGATGAAAA TGACGAAGGC TCTGTAGACA ACACTTTAGG AAGTGATAGA 
AGTAACGAGT TGGAAATACG GGGAAAATAT GTTGTTGTAC CAGAAACTTC ACAAGATATG 
GCCTTCAAAT GTCCAATTTG TAAGGAAACC GTTACTGGCG TTTATGATGA AGAATCTGGT 
GAATGGGTTT GGAAAAATAC TATTGAAGTG AATGGCAAAT ATTTCCACTC GACATGTTAT 
CATGAAACGT CGCAAAATTC AAGCAAATCT AATAGTGGCA AGGTCGGTTT GGATGACTTA 
AAGAAATTGG TCACAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PCF11 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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