SIR4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SIR4
Entrez Gene ID	851813
Full Name	chromatin-silencing protein SIR4
Synonyms	ASD1,STE9,UTH2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180535.1	NP_010513.1	chromatin-silencing protein SIR4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the silencing factor Sir4, NE-associated Esc1, the SUMO E3 ligase Siz2, and the nuclear pore complex (NPC) protein Nup170-physically and functionally interact with one another and a subset of NPC components (nucleoporins or Nups).
Title: Yeast silencing factor Sir4 and a subset of nucleoporins form a complex distinct from nuclear pore complexes.

Repair of UV-induced DNA lesions in Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.
Title: Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.

nse3-1/ sir4 Delta double mutants show additive defects for telomere shortening and TPE indicating the contribution of Smc5/6 to telomere homeostasis is only in partial overlap with SIR factor silencing. These findings support a role for Smc5/6 in telomere maintenance that is separate from its canonical role(s) in HR-mediated events during replication and telomere elongation.
Title: Smc5/6 Is a Telomere-Associated Complex that Regulates Sir4 Binding and TPE.

silencing requires Sir2, a highly-conserved NAD(+)-dependent histone deacetylase. At locations other than the rDNA, silencing also requires additional Sir proteins, Sir1, Sir3, and Sir4 that together form a repressive heterochromatin-like structure termed silent chromatin.
Title: The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.

data support a model whereby the demethylation of histone H3 K79 and changes in Sir4 abundance and availability define two rate-limiting steps that regulate de novo assembly of heterochromatin
Title: Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin.

The following SIR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01308	NM_001180535.1 Latest version!	Saccharomyces cerevisiae S288C chromatin-silencing protein SIR4 (SIR4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIR4 ( NM_001180535.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010513.1 Saccharomyces cerevisiae S288C chromatin-silencing protein SIR4 (SIR4), partial mRNA.

Price & Availability↑

CloneID	OSi01308
Clone ID Related Accession (Same CDS sequence)	NM_001180535.1
Accession Version	NM_001180535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chromatin-silencing protein SIR4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCAAATG ACAATAAGAC ACCCAATAGG TCCAGCACTC CCAAGTTTAC TAAAAAACCA 
GTAACCCCGA ATGATAAAAT TCCTGAAAGA GAAGAAAAAT CCAATGAAGT GAAGACACCT 
AAAATTCCAT TATTCACGTT TGCCAAAAGC AAAAACTATT CAAGGCCGTC AACCGCAATT 
CATACCTCAC CTCATCAACC AAGTGATGTA AAACCGACTT CCCATAAACA GTTGCAACAG 
CCAAAATCCT CACCACTGAA AAAAAATAAC TATAATTCTT TTCCTCACTC AAATCTGGAA 
AAAATAAGCA ACAGCAAACT ACTCTCCCTT CTTCGGTCCA AAACGTCAGC AGGAAGAATT 
GAATCAAACA ATCCTTCACA TGATGCTAGC AGGTCTCTAG CAAGTTTTGA ACAAACAGCA 
TTTTCCCGGC ATGCGCAGCA ACAAACTTCT ACCTTCAATT CAAAGCCTGT ACGTACCATA 
GTACCGATAT CAACATCACA AACCAATAAC TCATTTTTAT CAGGAGTAAA AAGCTTACTA 
AGTGAAGAGA AAATAAGGGA TTACTCTAAA GAGATTCTAG GCATAAACTT GGCAAACGAA 
CAGCCTGTTT TAGAGAAGCC ACTTAAAAAA GGATCAGCAG ACATCGGCGC CTCTGTGATT 
TCTTTGACCA AGGACAAATC TATCAGGAAA GATACCGTAG AAGAAAAAAA AGAAGAAAAG 
TTGAATATAG GTAAAAACTT TGCCCATTCT GATTCACTAT CAGTTCCGAA GGTTAGTGCC 
GGTGACAGTG GTATATCACC GGAGGAGAGC AAGGCAAGAA GTCCTGGAAT TGCAAAACCG 
AATGCCATAC AAACAGAAGT GTATGGAATA AATGAGGAAT CTACTAACGA GCGTTTAGAA 
ATAAATCAAG AAAAACCAGT AAAATTAGAT GAGAATAGTG CAAATAGTAC GGTCGCATCG 
GCCTTAGATA CCAATGGGAC GTCAGCCACG ACAGAAACTC TAACATCAAA GAAGATCGTT 
CCATCTCCAA AAAAAGTCGC CATTGATCAA GATAAAATAA CACTGCACGA TGAGAAAACA 
CTTGCACCTT CGAAGCATCA GCCTATAACA TCTGAACAAA AAATGAAGGA AGACGCCGAC 
CTCAAGAGGA TGGAAATCTT AAAGTCACCT CATTTGTCGA AAAGTCCAGC TGACAGACCT 
CAAGGGCGTA GAAACAGCCG AAATTTTTCC ACTAGAGATG AAGAAACTAC AAAACTTGCT 
TTTCTTGTTG AATATGAAGG CCAAGAAAAC AACTATAACT CTACTTCTCG AAGCACAGAA 
AAGAAAAATG ATATGAACAC TTCTGCAAAG AATAAAAATG GTGAAAACAA GAAAATTGGT 
AAGAGGCCGC CTGAAATCAT GAGCACTGAA GCTCACGTAA ACAAAGTAAC CGAAGAAACC 
ACAAAGCAGA TACAGAGTGT ACGAATAGAT GGTCGAAAAG TGCTTCAAAA AGTACAAGGA 
GAATCCCACA TTGATTCGAG AAACAATACC CTGAATGTTA CACCATCAAA GAGGCCCCAG 
CTAGGAGAAA TACCGAATCC TATGAAAAAG CATAAGCCTA ATGAAGGGCG AACTCCAAAT 
ATCTCAAACG GTACAATAAA CATCCAAAAG AAATTAGAGC CTAAGGAAAT TGTGCGAGAT 
ATTTTGCATA CGAAAGAATC ATCAAATGAG GCTAAGAAAA CTATTCAAAA CCCTTTAAAT 
AAATCACAAA ACACTGCTCT TCCTTCCACA CATAAAGTTA CACAAAAAAA AGATATAAAA 
ATTGGAACTA ATGACCTTTT TCAGGTTGAA TCTGCTCCAA AAATATCCTC AGAGATTGAC 
CGGGAGAATG TTAAATCAAA GGATGAACCG GTTTCCAAGG CTGTTGAAAG CAAATCTTTA 
TTAAATTTGT TTTCAAACGT ACTCAAGGCT CCTTTCATTA AAAGTGAAAG CAAACCTTTT 
TCTAGTGATG CTCTGTCAAA AGAAAAAGCC AATTTTTTGG AAACTATCGC TTCCACTGAA 
AAGCCAGAAA ATAAGACTGA TAAGGTGTCT CTATCTCAGC CAGTTAGTGC AAGTAAGCAT 
GAGTATAGCG ATAATTTTCC AGTTTCTCTA TCTCAACCTT CAAAGAAATC TTTCGCAAAT 
CATACAGAGG ATGAGCAAAT TGAAAAAAAG AAGATCTGCC GTGGGAGAAT GAATACGATA 
ATAACTCACC CGGGAAAAAT GGAGCTGGTA TACGTGTCCG ACTCAGACGA TTCTTCTTCA 
GATAATGATA GCCTAACTGA CTTGGAAAGT TTAAGCTCTG GTGAATCAAA TGAAATCAAA 
GTAACTAATG ATTTAGATAC AAGTGCTGAA AAGGACCAAA TTCAGGCAGG CAAATGGTTT 
GATCCTGTAT TGGATTGGCG AAAATCTGAT CGTGAATTGA CGAAAAACAT TCTTTGGAGG 
ATAGCTGATA AAACGACATA CGATAAAGAA ACAATAACTG ACCTTATTGA ACAAGGGATC 
CCAAAACATA GTTATTTAAG TGGCAATCCA TTAACTAGTG TGACTAACGA CATTTGCTCT 
GTTGAAAACT ATGAAACATC AAGTGCTTTC TTTTACCAAC AAGTGCACAA GAAGGACAGA 
TTACAATATT TGCCATTATA TGCAGTTTCT ACATTTGAAA ACACAAATAA CACTGAAAAA 
AATGATGTGA CCAATAAAAA TATCAATATA GGGAAACATA GCCAAGAACA AAATTCTTCT 
TCCGCTAAAC CATCCCAAAT TCCAACCGTG TCTTCTCCAT TAGGATTCGA AGAAACAAAG 
CTAAGTACCA CGCCTACTAA AAGCAATAGA AGAGTGTCGC ATAGTGATAC TAATTCTAGC 
AAACCCAAAA ACACGAAGGA GAACCTTTCA AAAAGCTCTT GGAGGCAAGA ATGGCTTGCA 
AATTTGAAAC TTATTTCCGT TTCGTTGGTT GATGAGTTCC CTTCGGAGCT TTCCGACAGT 
GATAGACAAA TAATTAACGA AAAAATGCAG TTACTTAAAG ATATATTTGC TAACAACCTT 
AAATCAGCAA TTTCCAATAA TTTTAGAGAG AGTGACATCA TTATACTGAA AGGTGAAATA 
GAAGATTACC CAATGAGTTC TGAAATTAAG ATTTACTACA ACGAATTACA GAACAAGCCT 
GATGCAAAAA AAGCCAGATT TTGGTCATTT ATGAAGACTC AGAGATTTGT TTCCAACATG 
GGATTTGATA TTCAGAAGTC CTGTGAACCT GTTTCTATAT CCACGTCGGT CAAACCACAT 
GTGGTCGAGC CCGAGCATAT GGCAGATGCC AAAATTATGC CTAAGGATAT ACTGCAAATT 
ACAAAAAAAC CGTTAATGGT TAAAAATGTG AAGCCTTCTT CTCCGCCAGA TGTGAAGTCT 
TTGGTGCAGC TGAGCACAAT GGAAACGAAA ACCCTACCAG AAAAGAAGCA ATTCGACAGT 
ATTTTTAATT CTAACAAAGC AAAAATAATC CCTGGAAATG GGAAGCACGC ATCAGAAAAC 
ATCTCACTCT CTTTCTCAAG ACCTGCGTCC TACGGCTATT TTTCTGTTGG AAAAAGGGTT 
CCAATCGTTG AAGATCGTCG AGTGAAACAA CTCGACGATA TAACAGACAG TAATACAACA 
GAAATTTTAA CTAGTGTTGA CGTTTTAGGA ACACATTCAC AAACTGGTAC TCAACAATCA 
AATATGTACA CATCAACCCA AAAAACAGAA CTTGAAATTG ATAATAAGGA TAGTGTCACC 
GAATGTTCGA AGGACATGAA AGAAGATGGT CTTTCCTTTG TGGATATCGT TTTGTCGAAA 
GCAGCATCGG CGCTGGATGA AAAGGAAAAA CAATTGGCTG TTGCAAATGA AATTATTCGG 
TCTTTGTCAG ATGAAGTTAT GAGGAATGAA ATTAGAATAA CTTCACTTCA AGGTGATTTA 
ACTTTTACAA AGAAATGTCT TGAAAATGCG AGAAGTCAAA TATCTGAAAA AGATGCTAAA 
ATTAACAAAT TGATGGAAAA AGATTTTCAA GTGAATAAGG AGATAAAACC GTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010513.1
CDS	1..4077
Translation	MPNDNKTPNRSSTPKFTKKPVTPNDKIPEREEKSNEVKTPKIPLFTFAKSKNYSRPSTAIHTSPHQPSDVKPTSHKQLQQPKSSPLKKNNYNSFPHSNLEKISNSKLLSLLRSKTSAGRIESNNPSHDASRSLASFEQTAFSRHAQQQTSTFNSKPVRTIVPISTSQTNNSFLSGVKSLLSEEKIRDYSKEILGINLANEQPVLEKPLKKGSADIGASVISLTKDKSIRKDTVEEKKEEKLNIGKNFAHSDSLSVPKVSAGDSGISPEESKARSPGIAKPNAIQTEVYGINEESTNERLEINQEKPVKLDENSANSTVASALDTNGTSATTETLTSKKIVPSPKKVAIDQDKITLHDEKTLAPSKHQPITSEQKMKEDADLKRMEILKSPHLSKSPADRPQGRRNSRNFSTRDEETTKLAFLVEYEGQENNYNSTSRSTEKKNDMNTSAKNKNGENKKIGKRPPEIMSTEAHVNKVTEETTKQIQSVRIDGRKVLQKVQGESHIDSRNNTLNVTPSKRPQLGEIPNPMKKHKPNEGRTPNISNGTINIQKKLEPKEIVRDILHTKESSNEAKKTIQNPLNKSQNTALPSTHKVTQKKDIKIGTNDLFQVESAPKISSEIDRENVKSKDEPVSKAVESKSLLNLFSNVLKAPFIKSESKPFSSDALSKEKANFLETIASTEKPENKTDKVSLSQPVSASKHEYSDNFPVSLSQPSKKSFANHTEDEQIEKKKICRGRMNTIITHPGKMELVYVSDSDDSSSDNDSLTDLESLSSGESNEIKVTNDLDTSAEKDQIQAGKWFDPVLDWRKSDRELTKNILWRIADKTTYDKETITDLIEQGIPKHSYLSGNPLTSVTNDICSVENYETSSAFFYQQVHKKDRLQYLPLYAVSTFENTNNTEKNDVTNKNINIGKHSQEQNSSSAKPSQIPTVSSPLGFEETKLSTTPTKSNRRVSHSDTNSSKPKNTKENLSKSSWRQEWLANLKLISVSLVDEFPSELSDSDRQIINEKMQLLKDIFANNLKSAISNNFRESDIIILKGEIEDYPMSSEIKIYYNELQNKPDAKKARFWSFMKTQRFVSNMGFDIQKSCEPVSISTSVKPHVVEPEHMADAKIMPKDILQITKKPLMVKNVKPSSPPDVKSLVQLSTMETKTLPEKKQFDSIFNSNKAKIIPGNGKHASENISLSFSRPASYGYFSVGKRVPIVEDRRVKQLDDITDSNTTEILTSVDVLGTHSQTGTQQSNMYTSTQKTELEIDNKDSVTECSKDMKEDGLSFVDIVLSKAASALDEKEKQLAVANEIIRSLSDEVMRNEIRITSLQGDLTFTKKCLENARSQISEKDAKINKLMEKDFQVNKEIKPY

Target ORF information:

RefSeq Version	NM_001180535.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chromatin-silencing protein SIR4 (SIR4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180535.1

ORF Insert Sequence:

ATGCCAAATG ACAATAAGAC ACCCAATAGG TCCAGCACTC CCAAGTTTAC TAAAAAACCA 
GTAACCCCGA ATGATAAAAT TCCTGAAAGA GAAGAAAAAT CCAATGAAGT GAAGACACCT 
AAAATTCCAT TATTCACGTT TGCCAAAAGC AAAAACTATT CAAGGCCGTC AACCGCAATT 
CATACCTCAC CTCATCAACC AAGTGATGTA AAACCGACTT CCCATAAACA GTTGCAACAG 
CCAAAATCCT CACCACTGAA AAAAAATAAC TATAATTCTT TTCCTCACTC AAATCTGGAA 
AAAATAAGCA ACAGCAAACT ACTCTCCCTT CTTCGGTCCA AAACGTCAGC AGGAAGAATT 
GAATCAAACA ATCCTTCACA TGATGCTAGC AGGTCTCTAG CAAGTTTTGA ACAAACAGCA 
TTTTCCCGGC ATGCGCAGCA ACAAACTTCT ACCTTCAATT CAAAGCCTGT ACGTACCATA 
GTACCGATAT CAACATCACA AACCAATAAC TCATTTTTAT CAGGAGTAAA AAGCTTACTA 
AGTGAAGAGA AAATAAGGGA TTACTCTAAA GAGATTCTAG GCATAAACTT GGCAAACGAA 
CAGCCTGTTT TAGAGAAGCC ACTTAAAAAA GGATCAGCAG ACATCGGCGC CTCTGTGATT 
TCTTTGACCA AGGACAAATC TATCAGGAAA GATACCGTAG AAGAAAAAAA AGAAGAAAAG 
TTGAATATAG GTAAAAACTT TGCCCATTCT GATTCACTAT CAGTTCCGAA GGTTAGTGCC 
GGTGACAGTG GTATATCACC GGAGGAGAGC AAGGCAAGAA GTCCTGGAAT TGCAAAACCG 
AATGCCATAC AAACAGAAGT GTATGGAATA AATGAGGAAT CTACTAACGA GCGTTTAGAA 
ATAAATCAAG AAAAACCAGT AAAATTAGAT GAGAATAGTG CAAATAGTAC GGTCGCATCG 
GCCTTAGATA CCAATGGGAC GTCAGCCACG ACAGAAACTC TAACATCAAA GAAGATCGTT 
CCATCTCCAA AAAAAGTCGC CATTGATCAA GATAAAATAA CACTGCACGA TGAGAAAACA 
CTTGCACCTT CGAAGCATCA GCCTATAACA TCTGAACAAA AAATGAAGGA AGACGCCGAC 
CTCAAGAGGA TGGAAATCTT AAAGTCACCT CATTTGTCGA AAAGTCCAGC TGACAGACCT 
CAAGGGCGTA GAAACAGCCG AAATTTTTCC ACTAGAGATG AAGAAACTAC AAAACTTGCT 
TTTCTTGTTG AATATGAAGG CCAAGAAAAC AACTATAACT CTACTTCTCG AAGCACAGAA 
AAGAAAAATG ATATGAACAC TTCTGCAAAG AATAAAAATG GTGAAAACAA GAAAATTGGT 
AAGAGGCCGC CTGAAATCAT GAGCACTGAA GCTCACGTAA ACAAAGTAAC CGAAGAAACC 
ACAAAGCAGA TACAGAGTGT ACGAATAGAT GGTCGAAAAG TGCTTCAAAA AGTACAAGGA 
GAATCCCACA TTGATTCGAG AAACAATACC CTGAATGTTA CACCATCAAA GAGGCCCCAG 
CTAGGAGAAA TACCGAATCC TATGAAAAAG CATAAGCCTA ATGAAGGGCG AACTCCAAAT 
ATCTCAAACG GTACAATAAA CATCCAAAAG AAATTAGAGC CTAAGGAAAT TGTGCGAGAT 
ATTTTGCATA CGAAAGAATC ATCAAATGAG GCTAAGAAAA CTATTCAAAA CCCTTTAAAT 
AAATCACAAA ACACTGCTCT TCCTTCCACA CATAAAGTTA CACAAAAAAA AGATATAAAA 
ATTGGAACTA ATGACCTTTT TCAGGTTGAA TCTGCTCCAA AAATATCCTC AGAGATTGAC 
CGGGAGAATG TTAAATCAAA GGATGAACCG GTTTCCAAGG CTGTTGAAAG CAAATCTTTA 
TTAAATTTGT TTTCAAACGT ACTCAAGGCT CCTTTCATTA AAAGTGAAAG CAAACCTTTT 
TCTAGTGATG CTCTGTCAAA AGAAAAAGCC AATTTTTTGG AAACTATCGC TTCCACTGAA 
AAGCCAGAAA ATAAGACTGA TAAGGTGTCT CTATCTCAGC CAGTTAGTGC AAGTAAGCAT 
GAGTATAGCG ATAATTTTCC AGTTTCTCTA TCTCAACCTT CAAAGAAATC TTTCGCAAAT 
CATACAGAGG ATGAGCAAAT TGAAAAAAAG AAGATCTGCC GTGGGAGAAT GAATACGATA 
ATAACTCACC CGGGAAAAAT GGAGCTGGTA TACGTGTCCG ACTCAGACGA TTCTTCTTCA 
GATAATGATA GCCTAACTGA CTTGGAAAGT TTAAGCTCTG GTGAATCAAA TGAAATCAAA 
GTAACTAATG ATTTAGATAC AAGTGCTGAA AAGGACCAAA TTCAGGCAGG CAAATGGTTT 
GATCCTGTAT TGGATTGGCG AAAATCTGAT CGTGAATTGA CGAAAAACAT TCTTTGGAGG 
ATAGCTGATA AAACGACATA CGATAAAGAA ACAATAACTG ACCTTATTGA ACAAGGGATC 
CCAAAACATA GTTATTTAAG TGGCAATCCA TTAACTAGTG TGACTAACGA CATTTGCTCT 
GTTGAAAACT ATGAAACATC AAGTGCTTTC TTTTACCAAC AAGTGCACAA GAAGGACAGA 
TTACAATATT TGCCATTATA TGCAGTTTCT ACATTTGAAA ACACAAATAA CACTGAAAAA 
AATGATGTGA CCAATAAAAA TATCAATATA GGGAAACATA GCCAAGAACA AAATTCTTCT 
TCCGCTAAAC CATCCCAAAT TCCAACCGTG TCTTCTCCAT TAGGATTCGA AGAAACAAAG 
CTAAGTACCA CGCCTACTAA AAGCAATAGA AGAGTGTCGC ATAGTGATAC TAATTCTAGC 
AAACCCAAAA ACACGAAGGA GAACCTTTCA AAAAGCTCTT GGAGGCAAGA ATGGCTTGCA 
AATTTGAAAC TTATTTCCGT TTCGTTGGTT GATGAGTTCC CTTCGGAGCT TTCCGACAGT 
GATAGACAAA TAATTAACGA AAAAATGCAG TTACTTAAAG ATATATTTGC TAACAACCTT 
AAATCAGCAA TTTCCAATAA TTTTAGAGAG AGTGACATCA TTATACTGAA AGGTGAAATA 
GAAGATTACC CAATGAGTTC TGAAATTAAG ATTTACTACA ACGAATTACA GAACAAGCCT 
GATGCAAAAA AAGCCAGATT TTGGTCATTT ATGAAGACTC AGAGATTTGT TTCCAACATG 
GGATTTGATA TTCAGAAGTC CTGTGAACCT GTTTCTATAT CCACGTCGGT CAAACCACAT 
GTGGTCGAGC CCGAGCATAT GGCAGATGCC AAAATTATGC CTAAGGATAT ACTGCAAATT 
ACAAAAAAAC CGTTAATGGT TAAAAATGTG AAGCCTTCTT CTCCGCCAGA TGTGAAGTCT 
TTGGTGCAGC TGAGCACAAT GGAAACGAAA ACCCTACCAG AAAAGAAGCA ATTCGACAGT 
ATTTTTAATT CTAACAAAGC AAAAATAATC CCTGGAAATG GGAAGCACGC ATCAGAAAAC 
ATCTCACTCT CTTTCTCAAG ACCTGCGTCC TACGGCTATT TTTCTGTTGG AAAAAGGGTT 
CCAATCGTTG AAGATCGTCG AGTGAAACAA CTCGACGATA TAACAGACAG TAATACAACA 
GAAATTTTAA CTAGTGTTGA CGTTTTAGGA ACACATTCAC AAACTGGTAC TCAACAATCA 
AATATGTACA CATCAACCCA AAAAACAGAA CTTGAAATTG ATAATAAGGA TAGTGTCACC 
GAATGTTCGA AGGACATGAA AGAAGATGGT CTTTCCTTTG TGGATATCGT TTTGTCGAAA 
GCAGCATCGG CGCTGGATGA AAAGGAAAAA CAATTGGCTG TTGCAAATGA AATTATTCGG 
TCTTTGTCAG ATGAAGTTAT GAGGAATGAA ATTAGAATAA CTTCACTTCA AGGTGATTTA 
ACTTTTACAA AGAAATGTCT TGAAAATGCG AGAAGTCAAA TATCTGAAAA AGATGCTAAA 
ATTAACAAAT TGATGGAAAA AGATTTTCAA GTGAATAAGG AGATAAAACC GTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SIR4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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