SAN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SAN1
Entrez Gene ID	851721
Full Name	ubiquitin-protein ligase SAN1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180450.3	NP_010427.3	ubiquitin-protein ligase SAN1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdc34 and Ubc1 are both sufficient for promoting San1 activity, San1 functions preferentially with Ubc1, including when both Ubc1 and Cdc34 are present.
Title: The San1 Ubiquitin Ligase Functions Preferentially with Ubiquitin-conjugating Enzyme Ubc1 during Protein Quality Control.

Study shows that San1 is involved in ubiquitylation and proteasomal degradation of the Spt16.
Title: Fine-Tuning of FACT by the Ubiquitin Proteasome System in Regulation of Transcriptional Elongation.

This study shows that an increasing molecular mass of substrates prevents nuclear San1-triggered proteasomal degradation but renders them susceptible to cytoplasmic Ubr1-triggered degradation.
Title: Molecular mass as a determinant for nuclear San1-dependent targeting of misfolded cytosolic proteins to proteasomal degradation.

Data indicate that the Hsp70, Ssa1p, facilitates an interaction between a novel misfolded substrate and San1p.
Title: Hsp70 targets a cytoplasmic quality control substrate to the San1p ubiquitin ligase.

Data indicate that the presence of key residues in San1 have been evolutionarily minimized to avoid self-destruction either in cis or in trans.
Title: Means of self-preservation: how an intrinsically disordered ubiquitin-protein ligase averts self-destruction.

The following SAN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SAN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01228	NM_001180450.3 Latest version!	Saccharomyces cerevisiae S288C ubiquitin-protein ligase SAN1 (SAN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SAN1 ( NM_001180450.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010427.3 Saccharomyces cerevisiae S288C ubiquitin-protein ligase SAN1 (SAN1), partial mRNA.

Price & Availability↑

CloneID	OSi01228
Clone ID Related Accession (Same CDS sequence)	NM_001180450.3
Accession Version	NM_001180450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ubiquitin-protein ligase SAN1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180450.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGAAA GTGGTCAAGA ACAAAACAGA GGCACAAATA CATCACCAAA TAATGCTGAA 
AATAATAATA ATTCAAATGC AGCTTCCGGT CCACTCAATG GTGGTGCTGA GCAAACAAGA 
AACATAACCG TTTCCATTCA GTATTCCTAT TTCACTCCGG AGAGATTAGC ACATTTGAGC 
AATATATCTA ATAATGATAA TAACGAAAAC AACAGCGCAG CATCCGGTAG CACGATTGCC 
AACGGTACTG GGCCCAGCTT TGGCATCGGA AATGGGGGCC ATCAACCCGA CGGTGCTCTT 
GTTTTATCTT TTCGTGATGT TCCTGCGTCT ACTCCACAAG ACCGTTTGAA CAGCTTCATA 
TCTGTTGCTG CTCAGCTTGC AATGGAGAGA TTCAACAGAC TATTAAATAG ACCAAAAGGA 
ATATCAAAGG ATGAGTTTGA TAAGCTCCCC GTTCTGCAAG TTTCTGATCT ACCCAAGGCC 
GAAGGGCCCT TATGTAGTAT ATGTTATGAC GAATATGAAG ATGAAGTTGA TTCAACTAAA 
GCAAAAAGAA AAAGGGATTC TGAAAATGAG GAGGAATCTG AAGGAACAAA AAAAAGGAAG 
GATAATGAAG GTGCGCCCCT ACGCACAACC GCCGATAATG ACAGTAACCC ATCGATTACA 
AATGCTACGG TTGTTGAACC GCCTTCTATT CCTCTCACTG AACAACAACG CACTCTCAAT 
GATGAAGAAA CAAATCCAAG CTACAAACAC TCACCAATCA AGTTACCTTG TGGCCACATT 
TTTGGGAGGG AATGTATCTA CAAATGGTCA AGATTAGAAA ATTCTTGTCC CCTTTGTAGA 
CAAAAGATCA GCGAATCTGT AGGTGTTCAA CGTGCAGCCC AACAAGATAC GGATGAAGTA 
GCAGCTAACG AAGCTGCTTT TGAACGTATT AGACGAGTTT TATACGACCC AACTGCAGTG 
AATAGCACTA ACGAAAATAG TTCCGCCCCA TCCGAAAACA CGTCCAATAC AACGGTTCCC 
ACTATCGGAA ATGCAAGTTC TGGCGAACAG ATGTTATCAA GAACAGGCTT TTTTTTAGTG 
CCTCAAAACG GCCAACCTTT ACACAATCCA GTCCGTTTAC CGCCTAATGA TAGCGATAGA 
AACGGTGTCA ACGGGCCGAG CTCAACTACT CAAAATCCAC CCTCTAATTC TGGTGGTTCG 
AATAACAATC AAAGTCCGCG CTGGGTTCCC ATCCCTTTGA CTTTGTTCCA ATTCCACAGT 
CCAAATCCGA ATCCTTCCGC TTCTGATTCT TCGGCAAGCC CATCAGCAGC GAACGGTCCA 
AACTCAAATA ACACTAGTAG TGACGCTACA GACCCTCACC ACAACAGACT AAGAGCCGTT 
TTGGATCACA TATTCAACGT TGCTCAGAGG GGAACTTCTG ATACCTCTGC AACAACAGCA 
CCCGGAGCAC AAACTGTTCA CAACCAAGGA CGTAATGACT CATCGTCCTC TGATACAACG 
CAAGGAAGTT CCTTTTTGGA AAATATTTCA CGATTAACAG GCCATTTTAC GAATGGCTCA 
AGAGACAACA ATAACGACAA TAACCATAGC AATGATCAAC AACGAGGTGG AAGTACTGGT 
GAGAACAACA GAAATAACTT GTTTTCCTCC GGTGTTGCCA GTTATAGAAA TCAAAATGGT 
GATGTTACTA CCGTCGAACT ACGCAACAAC AATTCTGCTG CCTTTCCTCC TACAGACGAA 
AATCCCTCTC AAGGCCAAGG TTCAAGCAGT TCGGACACCA CCATTCATAA CGACGTCCCT 
AATGATAACA ATGAGCAACG ATCATCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010427.3
CDS	1..1833
Translation	MSESGQEQNRGTNTSPNNAENNNNSNAASGPLNGGAEQTRNITVSIQYSYFTPERLAHLSNISNNDNNENNSAASGSTIANGTGPSFGIGNGGHQPDGALVLSFRDVPASTPQDRLNSFISVAAQLAMERFNRLLNRPKGISKDEFDKLPVLQVSDLPKAEGPLCSICYDEYEDEVDSTKAKRKRDSENEEESEGTKKRKDNEGAPLRTTADNDSNPSITNATVVEPPSIPLTEQQRTLNDEETNPSYKHSPIKLPCGHIFGRECIYKWSRLENSCPLCRQKISESVGVQRAAQQDTDEVAANEAAFERIRRVLYDPTAVNSTNENSSAPSENTSNTTVPTIGNASSGEQMLSRTGFFLVPQNGQPLHNPVRLPPNDSDRNGVNGPSSTTQNPPSNSGGSNNNQSPRWVPIPLTLFQFHSPNPNPSASDSSASPSAANGPNSNNTSSDATDPHHNRLRAVLDHIFNVAQRGTSDTSATTAPGAQTVHNQGRNDSSSSDTTQGSSFLENISRLTGHFTNGSRDNNNDNNHSNDQQRGGSTGENNRNNLFSSGVASYRNQNGDVTTVELRNNNSAAFPPTDENPSQGQGSSSSDTTIHNDVPNDNNEQRSSQ

Target ORF information:

RefSeq Version	NM_001180450.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ubiquitin-protein ligase SAN1 (SAN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180450.3

ORF Insert Sequence:

ATGAGTGAAA GTGGTCAAGA ACAAAACAGA GGCACAAATA CATCACCAAA TAATGCTGAA 
AATAATAATA ATTCAAATGC AGCTTCCGGT CCACTCAATG GTGGTGCTGA GCAAACAAGA 
AACATAACCG TTTCCATTCA GTATTCCTAT TTCACTCCGG AGAGATTAGC ACATTTGAGC 
AATATATCTA ATAATGATAA TAACGAAAAC AACAGCGCAG CATCCGGTAG CACGATTGCC 
AACGGTACTG GGCCCAGCTT TGGCATCGGA AATGGGGGCC ATCAACCCGA CGGTGCTCTT 
GTTTTATCTT TTCGTGATGT TCCTGCGTCT ACTCCACAAG ACCGTTTGAA CAGCTTCATA 
TCTGTTGCTG CTCAGCTTGC AATGGAGAGA TTCAACAGAC TATTAAATAG ACCAAAAGGA 
ATATCAAAGG ATGAGTTTGA TAAGCTCCCC GTTCTGCAAG TTTCTGATCT ACCCAAGGCC 
GAAGGGCCCT TATGTAGTAT ATGTTATGAC GAATATGAAG ATGAAGTTGA TTCAACTAAA 
GCAAAAAGAA AAAGGGATTC TGAAAATGAG GAGGAATCTG AAGGAACAAA AAAAAGGAAG 
GATAATGAAG GTGCGCCCCT ACGCACAACC GCCGATAATG ACAGTAACCC ATCGATTACA 
AATGCTACGG TTGTTGAACC GCCTTCTATT CCTCTCACTG AACAACAACG CACTCTCAAT 
GATGAAGAAA CAAATCCAAG CTACAAACAC TCACCAATCA AGTTACCTTG TGGCCACATT 
TTTGGGAGGG AATGTATCTA CAAATGGTCA AGATTAGAAA ATTCTTGTCC CCTTTGTAGA 
CAAAAGATCA GCGAATCTGT AGGTGTTCAA CGTGCAGCCC AACAAGATAC GGATGAAGTA 
GCAGCTAACG AAGCTGCTTT TGAACGTATT AGACGAGTTT TATACGACCC AACTGCAGTG 
AATAGCACTA ACGAAAATAG TTCCGCCCCA TCCGAAAACA CGTCCAATAC AACGGTTCCC 
ACTATCGGAA ATGCAAGTTC TGGCGAACAG ATGTTATCAA GAACAGGCTT TTTTTTAGTG 
CCTCAAAACG GCCAACCTTT ACACAATCCA GTCCGTTTAC CGCCTAATGA TAGCGATAGA 
AACGGTGTCA ACGGGCCGAG CTCAACTACT CAAAATCCAC CCTCTAATTC TGGTGGTTCG 
AATAACAATC AAAGTCCGCG CTGGGTTCCC ATCCCTTTGA CTTTGTTCCA ATTCCACAGT 
CCAAATCCGA ATCCTTCCGC TTCTGATTCT TCGGCAAGCC CATCAGCAGC GAACGGTCCA 
AACTCAAATA ACACTAGTAG TGACGCTACA GACCCTCACC ACAACAGACT AAGAGCCGTT 
TTGGATCACA TATTCAACGT TGCTCAGAGG GGAACTTCTG ATACCTCTGC AACAACAGCA 
CCCGGAGCAC AAACTGTTCA CAACCAAGGA CGTAATGACT CATCGTCCTC TGATACAACG 
CAAGGAAGTT CCTTTTTGGA AAATATTTCA CGATTAACAG GCCATTTTAC GAATGGCTCA 
AGAGACAACA ATAACGACAA TAACCATAGC AATGATCAAC AACGAGGTGG AAGTACTGGT 
GAGAACAACA GAAATAACTT GTTTTCCTCC GGTGTTGCCA GTTATAGAAA TCAAAATGGT 
GATGTTACTA CCGTCGAACT ACGCAACAAC AATTCTGCTG CCTTTCCTCC TACAGACGAA 
AATCCCTCTC AAGGCCAAGG TTCAAGCAGT TCGGACACCA CCATTCATAA CGACGTCCCT 
AATGATAACA ATGAGCAACG ATCATCACAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SAN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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