ORF » Species Summary » Saccharomyces cerevisiae S288C » AFR1 cDNA ORF clone

AFR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following AFR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01175 NM_001180393.1
Latest version!		 Saccharomyces cerevisiae S288C Afr1p (AFR1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    AFR1 ( NM_001180393.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010370.1 Saccharomyces cerevisiae S288C Afr1p (AFR1), partial mRNA.
    Price & Availability↑
    CloneID OSi01175
    Clone ID Related Accession (Same CDS sequence) NM_001180393.1
    Accession Version NM_001180393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Afr1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGGCT CATATCTATC TGCACAAGAA AACCAACCCA TACCGGAGCG ATTGATACCT 
    AGGTCGAACT CAACTTCGAA TTTGTTTGCT TTGTCTAGTA CATTCTCTAA ACTCAATGTT 
    AGGAATGATG CTGATTACAA TTATTCGAAC CCGAATAAGA AGAGACACAT TTATAGTGGC 
    GAGATAGACT GTAGGTCTGT GACAGCAGCT AGAAAATTTC CAGTAAGGTC CTGTTCAATG 
    ACAGCCGCCC AGCAAAGAAA GAGAACTGCG CTATTCACAG TTAGGGAAAG AAACTCTTAC 
    CATGAGGGGT TCAATAATGA TCAAGATTAT GTGAGCCAAT ACCAGAAGCC ACAGTACACT 
    TTCGGAGTAT ATAAAGAATT AACACCTTAT CAACTGCAGC GCTCTAAAAT GAAAAGAAGC 
    TTTCAATTTC CCAATGGCGA AATATACAAG CCAAAACTTG ATGGAAAATG CACCCACAGC 
    TTGAAAAAGC CTGAATTAAA TTCAAGAGAC TCTTCTTTAT TCAAATTCAG CGAGAAGAAA 
    GGTCGCAATT TATCAAAAGA TTTTGTGGGG CCTCATAATG GGACATCAGT CATACATATT 
    CCTCCCAATG ATACAGGCTA CGGTGTAAAT AGCTTGGAGC TGAACACTTC AGTTCCTTCA 
    ACAATAAAAA GTTCGGTTTC ATCAACCTCT CCGATATCTG CTGTTAATAC CCTGACCAGC 
    TTGCCAGAAA GTCAAACTGA TGATGATGAC GGTTATGAAA ATAAAACTGT AACTATATCT 
    TATTGCTTCG AAAATACGGT AAATGAAAAA CATGGTAGCC ATATAGAAAA ATTGGATTTA 
    TCAACTAAGG AAAAAACTAA GCCAACAACT AATTCAGGCC TATTTGATAG AAAGAAAAAG 
    ACTATTCTGG GCACTGAGAA ATATAGATGT ATAAAGTCAC AATCGAAGTT GAAACTGGGT 
    TCTGTTTTGA AGAAATTATG GCGTACTTCA GGAAACTCGA ATACAAAGCA TGGAAAAAAG 
    GATACAAAAA GAAGGAGAAT TCCTATCGAT GATATGGTTA CGCATTCTGA TGGAAATTCT 
    GAAGCTGAGA ATGATATTGA ATTAATGGAT GCAAATCTGG ACGGCATTGA ATTTGACGAC 
    GACGAAACTC TAATGGATAC AGATTCAATT TTTGATGATC TGTTGTCCAA GGAAAATGAT 
    AAATACGATT TGAGGCGAAG GCAATTAGAA ATACGACAAA AGCTCCACGA GACCTCGCAC 
    AACGACGATG GCAAGGTTTC TTTTCGAGAT ACCGAAAAGC ATAATGTCAA TGAAGGTTTG 
    ATCGACAAGA CCATAATCGA AGAATTTTCA AAGCTCGGTG AATATATAAT CGACACGAGA 
    AATCAACCAC CACCAAGATC GTCCAAGAGG CCTTCATTAG ATGACAATGA ATCTGCACGA 
    TACTTCTACA ACATATCGAC AGATTTGCGA CAGTCTCTGT CAGGTCCAAT AAGCTTACCA 
    ATGCATGTTG GGAACGACAT GGTAAATAGG TTGAGAAATG ACTGGGAGTA TATCAGATTC 
    GAAGATAGAA GGAATTCGCT GCCAGATTCC TCATTTGATA AAGTCGAAAC ACCTCCAAAA 
    CCAATAAAAA AAGATGTCCG GTTTGCAAAA GAAGTATGTT TAGCAAGCAC TTGGTCATCG 
    AATGCATATG AGAGAGCTAA TCCGGAATTC ATTATGAATC GCCACAGATT GTTGTGGATG 
    ATGAAAGTAC ATCCAAGCAT GAATAGTGCC ATGAATGAGA TCAAACTTGA ACTTAACTCT 
    TACAAAAAAA ATGAGATGGT TGTCCATGAG AACAGCAAGT GCTTTACACA TTATTTAATT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010370.1
    CDS1..1863
    Translation

    Target ORF information:

    RefSeq Version NM_001180393.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Afr1p (AFR1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001180393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGGGCT CATATCTATC TGCACAAGAA AACCAACCCA TACCGGAGCG ATTGATACCT 
    AGGTCGAACT CAACTTCGAA TTTGTTTGCT TTGTCTAGTA CATTCTCTAA ACTCAATGTT 
    AGGAATGATG CTGATTACAA TTATTCGAAC CCGAATAAGA AGAGACACAT TTATAGTGGC 
    GAGATAGACT GTAGGTCTGT GACAGCAGCT AGAAAATTTC CAGTAAGGTC CTGTTCAATG 
    ACAGCCGCCC AGCAAAGAAA GAGAACTGCG CTATTCACAG TTAGGGAAAG AAACTCTTAC 
    CATGAGGGGT TCAATAATGA TCAAGATTAT GTGAGCCAAT ACCAGAAGCC ACAGTACACT 
    TTCGGAGTAT ATAAAGAATT AACACCTTAT CAACTGCAGC GCTCTAAAAT GAAAAGAAGC 
    TTTCAATTTC CCAATGGCGA AATATACAAG CCAAAACTTG ATGGAAAATG CACCCACAGC 
    TTGAAAAAGC CTGAATTAAA TTCAAGAGAC TCTTCTTTAT TCAAATTCAG CGAGAAGAAA 
    GGTCGCAATT TATCAAAAGA TTTTGTGGGG CCTCATAATG GGACATCAGT CATACATATT 
    CCTCCCAATG ATACAGGCTA CGGTGTAAAT AGCTTGGAGC TGAACACTTC AGTTCCTTCA 
    ACAATAAAAA GTTCGGTTTC ATCAACCTCT CCGATATCTG CTGTTAATAC CCTGACCAGC 
    TTGCCAGAAA GTCAAACTGA TGATGATGAC GGTTATGAAA ATAAAACTGT AACTATATCT 
    TATTGCTTCG AAAATACGGT AAATGAAAAA CATGGTAGCC ATATAGAAAA ATTGGATTTA 
    TCAACTAAGG AAAAAACTAA GCCAACAACT AATTCAGGCC TATTTGATAG AAAGAAAAAG 
    ACTATTCTGG GCACTGAGAA ATATAGATGT ATAAAGTCAC AATCGAAGTT GAAACTGGGT 
    TCTGTTTTGA AGAAATTATG GCGTACTTCA GGAAACTCGA ATACAAAGCA TGGAAAAAAG 
    GATACAAAAA GAAGGAGAAT TCCTATCGAT GATATGGTTA CGCATTCTGA TGGAAATTCT 
    GAAGCTGAGA ATGATATTGA ATTAATGGAT GCAAATCTGG ACGGCATTGA ATTTGACGAC 
    GACGAAACTC TAATGGATAC AGATTCAATT TTTGATGATC TGTTGTCCAA GGAAAATGAT 
    AAATACGATT TGAGGCGAAG GCAATTAGAA ATACGACAAA AGCTCCACGA GACCTCGCAC 
    AACGACGATG GCAAGGTTTC TTTTCGAGAT ACCGAAAAGC ATAATGTCAA TGAAGGTTTG 
    ATCGACAAGA CCATAATCGA AGAATTTTCA AAGCTCGGTG AATATATAAT CGACACGAGA 
    AATCAACCAC CACCAAGATC GTCCAAGAGG CCTTCATTAG ATGACAATGA ATCTGCACGA 
    TACTTCTACA ACATATCGAC AGATTTGCGA CAGTCTCTGT CAGGTCCAAT AAGCTTACCA 
    ATGCATGTTG GGAACGACAT GGTAAATAGG TTGAGAAATG ACTGGGAGTA TATCAGATTC 
    GAAGATAGAA GGAATTCGCT GCCAGATTCC TCATTTGATA AAGTCGAAAC ACCTCCAAAA 
    CCAATAAAAA AAGATGTCCG GTTTGCAAAA GAAGTATGTT TAGCAAGCAC TTGGTCATCG 
    AATGCATATG AGAGAGCTAA TCCGGAATTC ATTATGAATC GCCACAGATT GTTGTGGATG 
    ATGAAAGTAC ATCCAAGCAT GAATAGTGCC ATGAATGAGA TCAAACTTGA ACTTAACTCT 
    TACAAAAAAA ATGAGATGGT TGTCCATGAG AACAGCAAGT GCTTTACACA TTATTTAATT 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
    Nature387(6632 Suppl)75-8(1997 May)
    Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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