STN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STN1
Entrez Gene ID	851655
Full Name	Stn1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180390.1	NP_010367.1	Stn1p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data support a model that Stn1 and Ten1 can function in a Cdc13-independent manner and have implications for the function of CST components across eukaryotes.
Title: Interplay between nonsense-mediated mRNA decay and DNA damage response pathways reveals that Stn1 and Ten1 are the key CST telomere-cap components.

Data show that by controlling the timing of Cdc13 and Stn1 phosphorylations, Cdk1 regulates the temporal recruitment of telomerase complexes and Cdc13-Stn1-Ten1 (CST) complexes to the telomeres to facilitate telomere maintenance.
Title: Cdk1 regulates the temporal recruitment of telomerase and Cdc13-Stn1-Ten1 complex for telomere replication.

Dimerization of the OB2 domain of Cdc13 is required for proper Cdc13, Stn1, Ten1 (CST) assembly and productive telomere capping.
Title: Cdc13 OB2 dimerization required for productive Stn1 binding and efficient telomere maintenance.

the crystal structures of the C-terminal domain of the Saccharomyces cerevisiae Stn1 and the Schizosaccharomyces pombe Ten1 proteins are reported.
Title: Telomere capping proteins are structurally related to RPA with an additional telomere-specific domain.

The N terminus of Stn1 interacts with Ten1 and carries out its essential capping function. The C terminus of Stn1 binds both Cdc13 and Pol12.
Title: Distinct roles for yeast Stn1 in telomere capping and telomerase inhibition.

The following STN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01172	NM_001180390.1 Latest version!	Saccharomyces cerevisiae S288C Stn1p (STN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STN1 ( NM_001180390.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010367.1 Saccharomyces cerevisiae S288C Stn1p (STN1), partial mRNA.

Price & Availability↑

CloneID	OSi01172
Clone ID Related Accession (Same CDS sequence)	NM_001180390.1
Accession Version	NM_001180390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Stn1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATAAGT ACGGACATAT AGCTCATCAA GAAGGCGATG TTTGTTATTA TATTCCAAGG 
CTGTTCAAAT ACAACAGTTA TTATAGTGGG ACAGAGGACG TTCGAATATT TGTTGGAGAT 
TTAAAGTACC GGATGAGAGT TTCTTTGCAA ATTTGCGAAA AGTATTATGA TAGGCGGTTG 
TCGATGTTGT TCTGGAAAAA TCATCCTTTA CAGCAAATAC ACCTTATTGG CTGCATCATT 
GGACTCCAGT TCAAATGGAT TGGGAAACAA GAATACATTT TTTTTCAGTT GGACGATTGT 
ACTTCAGACT CTTCATTGGT GGGTTATACT AGTGACATGA GATTCTTAAC ATGTAAAGTT 
AAGAAGGATA GCATCCTATC ATGGGGGTTG AACATAACCG ATTTGATAGG CTTAACTCTT 
CATGTCTACG GGCAAGCAAG TTTGAACTAT CAAGAGCTTC AAGTTGAATA TTTAAGATTA 
TGCTACAGTT TAACGGAAGA AATAGATCAT TGGAAAATCA CAATGAACAT GCGTGAGCAA 
CTTGACACGC CTTGGTCCTT AAGCGATTTT GTAATTGGAG AATTGTTCAC GCAAGAGCAA 
GAGTGGACAC CTGAGACATC ACAGATTGAG GTAGTAAACC CGGATTTTGT AGGTATAGGG 
TACAAAACCC CAGAAAGTAA AAGAAATGAA ACTACTTTCA TCGAGCAACT GCAAGAAGAA 
CGCTTGAAGG ATGAATTAGA AATTATTAGT CCATACAATA GCACAGATAC TTCGAATAGT 
GTGCATTCCT TGTCATTTCG GTTTGTATCG TCGCTAAAGG ATTTTCCTGA AACTCACTTT 
TTGAATTCAG GTGATCAAAT TGACAACGGC AATGACGAAC AGTTAAAAAA ACTAGAATAT 
CAAAGCGCTA ATCTACCTGT GATGATTCCC AATAGGACAT CAGCAAAGTC AAACTTGATG 
TTGATCCTAT TAGGACTACA AATGAAAGAG ATTTCTAATT CTGACCTTTA CAAACTAAAG 
GAAGTACGCA GCGTAGTAAC TAGCTTAGCA TCGTTTTTGT TTCAACAACA AAATGTTGGC 
GTCATGAAAT CTTTTGACTC TTTGGAAAAG GAAGCGTTTC GAGACCTGGT TAATCGTCTA 
GTCAGCCAGG GTTTGATCGG ATTAAAAGAT AAAACAAGTG AGACATTTGA TTTGCTTCCT 
CTAAAAAATC TTTTTGAATA TGCTGAAAAA CGTATATCAG TTCTAATGAA GCTACAATGT 
TATACAGGCA CTGTGCAATT AAGTCATGTT CAGGAAAAGC TACATTTGCC GTACATAACA 
ACGAACGGCA TCGTTGATGT TTTCAAAGAA TGTCTTAAAA GGACAAAGAA ACAGTATCCT 
GAGGTACTAA AAAATTGGTG GATTGATCTT GATCCGAAGA ATGGTATGGA GGACCAAAAC 
AGTGGAATAT TATTACATTT GGAATACGCA GCAGCATATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010367.1
CDS	1..1485
Translation	MDKYGHIAHQEGDVCYYIPRLFKYNSYYSGTEDVRIFVGDLKYRMRVSLQICEKYYDRRLSMLFWKNHPLQQIHLIGCIIGLQFKWIGKQEYIFFQLDDCTSDSSLVGYTSDMRFLTCKVKKDSILSWGLNITDLIGLTLHVYGQASLNYQELQVEYLRLCYSLTEEIDHWKITMNMREQLDTPWSLSDFVIGELFTQEQEWTPETSQIEVVNPDFVGIGYKTPESKRNETTFIEQLQEERLKDELEIISPYNSTDTSNSVHSLSFRFVSSLKDFPETHFLNSGDQIDNGNDEQLKKLEYQSANLPVMIPNRTSAKSNLMLILLGLQMKEISNSDLYKLKEVRSVVTSLASFLFQQQNVGVMKSFDSLEKEAFRDLVNRLVSQGLIGLKDKTSETFDLLPLKNLFEYAEKRISVLMKLQCYTGTVQLSHVQEKLHLPYITTNGIVDVFKECLKRTKKQYPEVLKNWWIDLDPKNGMEDQNSGILLHLEYAAAYS

Target ORF information:

RefSeq Version	NM_001180390.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Stn1p (STN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180390.1

ORF Insert Sequence:

ATGGATAAGT ACGGACATAT AGCTCATCAA GAAGGCGATG TTTGTTATTA TATTCCAAGG 
CTGTTCAAAT ACAACAGTTA TTATAGTGGG ACAGAGGACG TTCGAATATT TGTTGGAGAT 
TTAAAGTACC GGATGAGAGT TTCTTTGCAA ATTTGCGAAA AGTATTATGA TAGGCGGTTG 
TCGATGTTGT TCTGGAAAAA TCATCCTTTA CAGCAAATAC ACCTTATTGG CTGCATCATT 
GGACTCCAGT TCAAATGGAT TGGGAAACAA GAATACATTT TTTTTCAGTT GGACGATTGT 
ACTTCAGACT CTTCATTGGT GGGTTATACT AGTGACATGA GATTCTTAAC ATGTAAAGTT 
AAGAAGGATA GCATCCTATC ATGGGGGTTG AACATAACCG ATTTGATAGG CTTAACTCTT 
CATGTCTACG GGCAAGCAAG TTTGAACTAT CAAGAGCTTC AAGTTGAATA TTTAAGATTA 
TGCTACAGTT TAACGGAAGA AATAGATCAT TGGAAAATCA CAATGAACAT GCGTGAGCAA 
CTTGACACGC CTTGGTCCTT AAGCGATTTT GTAATTGGAG AATTGTTCAC GCAAGAGCAA 
GAGTGGACAC CTGAGACATC ACAGATTGAG GTAGTAAACC CGGATTTTGT AGGTATAGGG 
TACAAAACCC CAGAAAGTAA AAGAAATGAA ACTACTTTCA TCGAGCAACT GCAAGAAGAA 
CGCTTGAAGG ATGAATTAGA AATTATTAGT CCATACAATA GCACAGATAC TTCGAATAGT 
GTGCATTCCT TGTCATTTCG GTTTGTATCG TCGCTAAAGG ATTTTCCTGA AACTCACTTT 
TTGAATTCAG GTGATCAAAT TGACAACGGC AATGACGAAC AGTTAAAAAA ACTAGAATAT 
CAAAGCGCTA ATCTACCTGT GATGATTCCC AATAGGACAT CAGCAAAGTC AAACTTGATG 
TTGATCCTAT TAGGACTACA AATGAAAGAG ATTTCTAATT CTGACCTTTA CAAACTAAAG 
GAAGTACGCA GCGTAGTAAC TAGCTTAGCA TCGTTTTTGT TTCAACAACA AAATGTTGGC 
GTCATGAAAT CTTTTGACTC TTTGGAAAAG GAAGCGTTTC GAGACCTGGT TAATCGTCTA 
GTCAGCCAGG GTTTGATCGG ATTAAAAGAT AAAACAAGTG AGACATTTGA TTTGCTTCCT 
CTAAAAAATC TTTTTGAATA TGCTGAAAAA CGTATATCAG TTCTAATGAA GCTACAATGT 
TATACAGGCA CTGTGCAATT AAGTCATGTT CAGGAAAAGC TACATTTGCC GTACATAACA 
ACGAACGGCA TCGTTGATGT TTTCAAAGAA TGTCTTAAAA GGACAAAGAA ACAGTATCCT 
GAGGTACTAA AAAATTGGTG GATTGATCTT GATCCGAAGA ATGGTATGGA GGACCAAAAC 
AGTGGAATAT TATTACATTT GGAATACGCA GCAGCATATT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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STN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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