VPS41 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS41
Entrez Gene ID	851653
Full Name	Vps41p
Synonyms	CVT8,FET2,SVL2,VAM2,VPL20
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180388.3	NP_010365.3	Vps41p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	VPS41	NP_010365.3
Magnaporthe oryzae (rice blast fungus)	MGG_03313	XP_003716647.1
Kluyveromyces lactis	KLLA0C11759g	XP_452725.1
Eremothecium gossypii	AGOS_AGR149W	NP_986815.1
Neurospora crassa	NCU05902	XP_959460.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results describe a phosphorylation-based switch mechanism, which allows the homotypic vacuole fusion protein sorting effector subunit Vps41 to operate in two distinct fusion events, namely endosome-vacuole and AP-3 vesicle-vacuole fusion.
Title: Phosphorylation of a membrane curvature-sensing motif switches function of the HOPS subunit Vps41 in membrane tethering.

Data show that although the AP-3 delta subunit, Apl5, binds Vps41 directly, this interaction occurs preferentially within the context of the HOPS docking complex.
Title: HOPS interacts with Apl5 at the vacuole membrane and is required for consumption of AP-3 transport vesicles.

Both Ypt7 and Yck3-mediated phosphorylation modulate the Vps41 localization to the endosome-vacuole junction.
Title: Vps41 phosphorylation and the Rab Ypt7 control the targeting of the HOPS complex to endosome-vacuole fusion sites.

Mutants in the V0-part of the V0/V1-ATPase accumulate Vps41.
Title: The vacuolar V1/V0-ATPase is involved in the release of the HOPS subunit Vps41 from vacuoles, vacuole fragmentation and fusion.

The following VPS41 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS41 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01170	NM_001180388.3 Latest version!	Saccharomyces cerevisiae S288C Vps41p (VPS41), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS41 ( NM_001180388.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010365.3 Saccharomyces cerevisiae S288C Vps41p (VPS41), partial mRNA.

Price & Availability↑

CloneID	OSi01170
Clone ID Related Accession (Same CDS sequence)	NM_001180388.3
Accession Version	NM_001180388.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Vps41p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180388.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTACAG ATAATCATCA GAATGATTCT GTTTTGGATC AACAATCAGG CGAACGAACA 
ATTGATGAGA GTAATTCCAT CTCTGACGAG AATAATGTTG ATAATAAGAG GGAAGACGTT 
AATGTAACTT CACCAACAAA AAGTGTTAGT TGCATATCAC AAGCGGAAAA TGGAGTAGCT 
TCTCGAACAG ATGAGAGTAC AATAACAGGA AGTGCGACGG ATGCAGAAAC AGGTGATGAT 
GATGATGATG ATGATGATGA CGATGATGAA GATGAGGATG ACGAAGATGA GCCCCCCTTA 
TTGAAGTACA CACGAATTAG TCAACTCCCG AAGAACTTTT TTCAACGAGA CTCGATCTCT 
TCATGTTTAT TTGGTGATAC TTTTTTTGCA TTTGGTACAC ACTCAGGCAT TCTACACTTA 
ACTACATGCG CGTTCGAACC CATTAAAACC ATCAAATGCC ATAGATCTTC GATACTTTGT 
ATTAACACTG ACGGAAAATA TTTTGCTACG GGATCTATTG ACGGGACAGT GATTATCGGA 
TCGATGGATG ATCCACAAAA CATTACACAA TACGATTTCA AAAGACCTAT AAACTCTGTA 
GCGTTGCACT CAAATTTTCA AGCATCCAGA ATGTTTGTTT CTGGTGGAAT GGCTGGCGAC 
GTTGTACTAT CTCAGAGAAA TTGGCTAGGA AATAGAATCG ACATCGTTTT AAACAAGAAG 
AAAAAGAAAA AAACAAGAAA AGATGACCTA TCCTCTGATA TGAAGGGCCC TATCATGGGA 
ATTTATACTA TGGGTGACCT TATATTATGG ATGGATGATG ATGGAATAAC ATTTTGCGAT 
GTTCCTACAA GATCACAGCT TTTGAATATT CCATTCCCCT CCAGAATTTT TAACGTACAG 
GATGTAAGGC CAGATCTATT TAGACCCCAT GTTCATTTTT TAGAAAGTGA TCGTGTCGTT 
ATCGGATGGG GATCTAATAT TTGGCTATTT AAAGTTTCCT TTACCAAGGA TTCAAATTCG 
ATCAAATCCG GTGACTCTAA TTCTCAAAGT AATAATATGA GTCATTTTAA CCCTACGACA 
AACATCGGAT CTCTCTTGTC TAGTGCTGCA TCGAGTTTTA GAGGGACACC TGATAAGAAA 
GTTGAATTAG AATGCCATTT TACTGTGTCA ATGTTGATTA CAGGGCTTGC ATCGTTTAAG 
GATGATCAGT TATTATGCCT AGGTTTTGAT ATAGATATTG AAGAGGAAGC AACCATAGAT 
GAAGATATGA AGGAGGGGAA GAATTTTAGC AAGCGGCCAG AAAACTTATT AGCCAAAGGG 
AATGCTCCAG AATTAAAAAT TGTTGATCTT TTTAATGGTG ATGAAATCTA TAATGATGAA 
GTTATCATGA AAAACTATGA AAAATTGTCT ATAAATGATT ATCACCTCGG TAAACATATA 
GATAAAACAA CACCAGAATA CTATTTGATA AGTTCCAACG ATGCCATCAG GGTTCAAGAG 
TTATCATTGA AGGATCATTT CGATTGGTTT ATGGAAAGAA AGCAATATTA TAAAGCTTGG 
AAAATCGGTA AATATGTAAT TGGATCAGAA GAGAGGTTTA GTATAGGGTT GAAATTCCTC 
AACTCTTTAG TAACTAAAAA AGATTGGGGT ACATTAGTGG ACCACTTAAA TATTATTTTC 
GAAGAAACTT TAAACTCATT GGATTCCAAC TCATATGATG TCACTCAAAA CGTGCTGAAA 
GAATGGGCAG ATATAATAGA GATTTTGATT ACATCAGGAA ATATTGTAGA AATTGCGCCT 
TTGATTCCCA AGAAACCTGC CTTAAGAAAG TCGGTATATG ACGATGTATT ACATTACTTT 
TTGGCCAATG ACATGATCAA TAAGTTTCAT GAGTATATTA CTAAGTGGGA TTTAAAGCTT 
TTTTCTGTGG AAGATTTCGA AGAAGAGCTA GAAACAAGAA TAGAGGCTGC TAGTGAACCT 
ACAGCTTCAT CGAAGGAAGA AGGGTCTAAT ATTACCTATA GAACTGAGTT GGTGCATTTA 
TATTTGAAAG AGAATAAATA TACCAAGGCT ATACCGCACC TTTTGAAAGC TAAGGACTTA 
AGAGCTTTAA CTATTATAAA AATACAAAAT TTATTGCCGC AGTATCTAGA CCAGATAGTT 
GATATCATTC TTTTACCATA TAAGGGTGAA ATTTCCCATA TTTCCAAACT ATCAATATTT 
GAAATTCAAA CAATTTTTAA CAAACCAATA GACCTGTTGT TTGAAAATAG ACACACCATA 
TCAGTCGCTA GAATATACGA AATATTTGAA CATGATTGTC CAAAAAGTTT CAAAAAGATT 
TTATTTTGTT ATTTAATAAA ATTTTTAGAT ACGGACGACT CGTTCATGAT AAGTCCTTAT 
GAAAATCAAC TAATTGAACT TTATTCTGAA TACGACCGAC AAAGCCTTCT GCCTTTTCTG 
CAAAAGCACA ATAACTATAA TGTAGAGAGT GCCATCGAAG TCTGTTCATC GAAACTTGGT 
TTATATAATG AACTGATCTA TTTGTGGGGG AAAATCGGTG AAACGAAGAA AGCATTGTCT 
TTGATTATTG ACGAGTTAAA AAACCCGCAA TTAGCTATAG ATTTTGTTAA AAACTGGGGT 
GATTCAGAAC TTTGGGAATT CATGATTAAC TATAGTCTTG ACAAACCTAA TTTTACTAAA 
GCCATTTTAA CATGTTCTGA TGAGACCAGC GAAATTTACC TAAAAGTGAT CAGAGGTATG 
TCCGATGATC TGCAAATTGA CAACCTGCAG GATATTATTA AACATATCGT TCAAGAAAAT 
TCATTAAGCT TGGAAGTTAG AGATAATATT TTGGTTATCA TTAATGATGA AACGAAAAAA 
TTTGCCAATG AATTCCTGAA AATAAGAAGT CAAGGTAAAT TGTTTCAAGT AGACGAAAGC 
GATATTGAAA TTAATGACGA CTTAAATGGT GTTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010365.3
CDS	1..2979
Translation	MTTDNHQNDSVLDQQSGERTIDESNSISDENNVDNKREDVNVTSPTKSVSCISQAENGVASRTDESTITGSATDAETGDDDDDDDDDDDEDEDDEDEPPLLKYTRISQLPKNFFQRDSISSCLFGDTFFAFGTHSGILHLTTCAFEPIKTIKCHRSSILCINTDGKYFATGSIDGTVIIGSMDDPQNITQYDFKRPINSVALHSNFQASRMFVSGGMAGDVVLSQRNWLGNRIDIVLNKKKKKKTRKDDLSSDMKGPIMGIYTMGDLILWMDDDGITFCDVPTRSQLLNIPFPSRIFNVQDVRPDLFRPHVHFLESDRVVIGWGSNIWLFKVSFTKDSNSIKSGDSNSQSNNMSHFNPTTNIGSLLSSAASSFRGTPDKKVELECHFTVSMLITGLASFKDDQLLCLGFDIDIEEEATIDEDMKEGKNFSKRPENLLAKGNAPELKIVDLFNGDEIYNDEVIMKNYEKLSINDYHLGKHIDKTTPEYYLISSNDAIRVQELSLKDHFDWFMERKQYYKAWKIGKYVIGSEERFSIGLKFLNSLVTKKDWGTLVDHLNIIFEETLNSLDSNSYDVTQNVLKEWADIIEILITSGNIVEIAPLIPKKPALRKSVYDDVLHYFLANDMINKFHEYITKWDLKLFSVEDFEEELETRIEAASEPTASSKEEGSNITYRTELVHLYLKENKYTKAIPHLLKAKDLRALTIIKIQNLLPQYLDQIVDIILLPYKGEISHISKLSIFEIQTIFNKPIDLLFENRHTISVARIYEIFEHDCPKSFKKILFCYLIKFLDTDDSFMISPYENQLIELYSEYDRQSLLPFLQKHNNYNVESAIEVCSSKLGLYNELIYLWGKIGETKKALSLIIDELKNPQLAIDFVKNWGDSELWEFMINYSLDKPNFTKAILTCSDETSEIYLKVIRGMSDDLQIDNLQDIIKHIVQENSLSLEVRDNILVIINDETKKFANEFLKIRSQGKLFQVDESDIEINDDLNGVL

Target ORF information:

RefSeq Version	NM_001180388.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Vps41p (VPS41), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180388.3

ORF Insert Sequence:

ATGACTACAG ATAATCATCA GAATGATTCT GTTTTGGATC AACAATCAGG CGAACGAACA 
ATTGATGAGA GTAATTCCAT CTCTGACGAG AATAATGTTG ATAATAAGAG GGAAGACGTT 
AATGTAACTT CACCAACAAA AAGTGTTAGT TGCATATCAC AAGCGGAAAA TGGAGTAGCT 
TCTCGAACAG ATGAGAGTAC AATAACAGGA AGTGCGACGG ATGCAGAAAC AGGTGATGAT 
GATGATGATG ATGATGATGA CGATGATGAA GATGAGGATG ACGAAGATGA GCCCCCCTTA 
TTGAAGTACA CACGAATTAG TCAACTCCCG AAGAACTTTT TTCAACGAGA CTCGATCTCT 
TCATGTTTAT TTGGTGATAC TTTTTTTGCA TTTGGTACAC ACTCAGGCAT TCTACACTTA 
ACTACATGCG CGTTCGAACC CATTAAAACC ATCAAATGCC ATAGATCTTC GATACTTTGT 
ATTAACACTG ACGGAAAATA TTTTGCTACG GGATCTATTG ACGGGACAGT GATTATCGGA 
TCGATGGATG ATCCACAAAA CATTACACAA TACGATTTCA AAAGACCTAT AAACTCTGTA 
GCGTTGCACT CAAATTTTCA AGCATCCAGA ATGTTTGTTT CTGGTGGAAT GGCTGGCGAC 
GTTGTACTAT CTCAGAGAAA TTGGCTAGGA AATAGAATCG ACATCGTTTT AAACAAGAAG 
AAAAAGAAAA AAACAAGAAA AGATGACCTA TCCTCTGATA TGAAGGGCCC TATCATGGGA 
ATTTATACTA TGGGTGACCT TATATTATGG ATGGATGATG ATGGAATAAC ATTTTGCGAT 
GTTCCTACAA GATCACAGCT TTTGAATATT CCATTCCCCT CCAGAATTTT TAACGTACAG 
GATGTAAGGC CAGATCTATT TAGACCCCAT GTTCATTTTT TAGAAAGTGA TCGTGTCGTT 
ATCGGATGGG GATCTAATAT TTGGCTATTT AAAGTTTCCT TTACCAAGGA TTCAAATTCG 
ATCAAATCCG GTGACTCTAA TTCTCAAAGT AATAATATGA GTCATTTTAA CCCTACGACA 
AACATCGGAT CTCTCTTGTC TAGTGCTGCA TCGAGTTTTA GAGGGACACC TGATAAGAAA 
GTTGAATTAG AATGCCATTT TACTGTGTCA ATGTTGATTA CAGGGCTTGC ATCGTTTAAG 
GATGATCAGT TATTATGCCT AGGTTTTGAT ATAGATATTG AAGAGGAAGC AACCATAGAT 
GAAGATATGA AGGAGGGGAA GAATTTTAGC AAGCGGCCAG AAAACTTATT AGCCAAAGGG 
AATGCTCCAG AATTAAAAAT TGTTGATCTT TTTAATGGTG ATGAAATCTA TAATGATGAA 
GTTATCATGA AAAACTATGA AAAATTGTCT ATAAATGATT ATCACCTCGG TAAACATATA 
GATAAAACAA CACCAGAATA CTATTTGATA AGTTCCAACG ATGCCATCAG GGTTCAAGAG 
TTATCATTGA AGGATCATTT CGATTGGTTT ATGGAAAGAA AGCAATATTA TAAAGCTTGG 
AAAATCGGTA AATATGTAAT TGGATCAGAA GAGAGGTTTA GTATAGGGTT GAAATTCCTC 
AACTCTTTAG TAACTAAAAA AGATTGGGGT ACATTAGTGG ACCACTTAAA TATTATTTTC 
GAAGAAACTT TAAACTCATT GGATTCCAAC TCATATGATG TCACTCAAAA CGTGCTGAAA 
GAATGGGCAG ATATAATAGA GATTTTGATT ACATCAGGAA ATATTGTAGA AATTGCGCCT 
TTGATTCCCA AGAAACCTGC CTTAAGAAAG TCGGTATATG ACGATGTATT ACATTACTTT 
TTGGCCAATG ACATGATCAA TAAGTTTCAT GAGTATATTA CTAAGTGGGA TTTAAAGCTT 
TTTTCTGTGG AAGATTTCGA AGAAGAGCTA GAAACAAGAA TAGAGGCTGC TAGTGAACCT 
ACAGCTTCAT CGAAGGAAGA AGGGTCTAAT ATTACCTATA GAACTGAGTT GGTGCATTTA 
TATTTGAAAG AGAATAAATA TACCAAGGCT ATACCGCACC TTTTGAAAGC TAAGGACTTA 
AGAGCTTTAA CTATTATAAA AATACAAAAT TTATTGCCGC AGTATCTAGA CCAGATAGTT 
GATATCATTC TTTTACCATA TAAGGGTGAA ATTTCCCATA TTTCCAAACT ATCAATATTT 
GAAATTCAAA CAATTTTTAA CAAACCAATA GACCTGTTGT TTGAAAATAG ACACACCATA 
TCAGTCGCTA GAATATACGA AATATTTGAA CATGATTGTC CAAAAAGTTT CAAAAAGATT 
TTATTTTGTT ATTTAATAAA ATTTTTAGAT ACGGACGACT CGTTCATGAT AAGTCCTTAT 
GAAAATCAAC TAATTGAACT TTATTCTGAA TACGACCGAC AAAGCCTTCT GCCTTTTCTG 
CAAAAGCACA ATAACTATAA TGTAGAGAGT GCCATCGAAG TCTGTTCATC GAAACTTGGT 
TTATATAATG AACTGATCTA TTTGTGGGGG AAAATCGGTG AAACGAAGAA AGCATTGTCT 
TTGATTATTG ACGAGTTAAA AAACCCGCAA TTAGCTATAG ATTTTGTTAA AAACTGGGGT 
GATTCAGAAC TTTGGGAATT CATGATTAAC TATAGTCTTG ACAAACCTAA TTTTACTAAA 
GCCATTTTAA CATGTTCTGA TGAGACCAGC GAAATTTACC TAAAAGTGAT CAGAGGTATG 
TCCGATGATC TGCAAATTGA CAACCTGCAG GATATTATTA AACATATCGT TCAAGAAAAT 
TCATTAAGCT TGGAAGTTAG AGATAATATT TTGGTTATCA TTAATGATGA AACGAAAAAA 
TTTGCCAATG AATTCCTGAA AATAAGAAGT CAAGGTAAAT TGTTTCAAGT AGACGAAAGC 
GATATTGAAA TTAATGACGA CTTAAATGGT GTTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VPS41 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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