DOA4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DOA4
Entrez Gene ID	851641
Full Name	ubiquitin-specific protease DOA4
Synonyms	DOS1,MUT4,NPI2,SSV7,UBP4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180377.3	NP_010354.3	ubiquitin-specific protease DOA4

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E10275g	XP_454413.1
Eremothecium gossypii	AGOS_AFR007W	NP_985554.2
Saccharomyces cerevisiae (baker's yeast)	DOA4	NP_010354.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Doa4 physically interacts with the ESCRT-III component Snf7 and preferentially cleaves Lys63-linked ubiquitin oligomers involved in membrane protein trafficking
Title: The N-terminal domains determine cellular localization and functions of the Doa4 and Ubp5 deubiquitinating enzymes.

Binding of Vps20 to the Doa4 N-terminus inhibits a non-catalytic function of Doa4 that promotes ILV formation.
Title: Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III.

identified a protein designated Rfu1 (regulator of free ubiquitin chains 1), which regulates intracellular concentrations of monomeric ubiquitins; functions as an inhibitor of Doa4
Title: An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis.

Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies.
Title: Dual mechanisms specify Doa4-mediated deubiquitination at multivesicular bodies.

Deubiquitination by Doa4 of cargo proteins and/or some components of the multivesicular body (MVB) sorting machinery is essential to correct sorting of cargoes into the MVB pathway.
Title: Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway.

The following DOA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01158	NM_001180377.3 Latest version!	Saccharomyces cerevisiae S288C ubiquitin-specific protease DOA4 (DOA4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOA4 ( NM_001180377.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010354.3 Saccharomyces cerevisiae S288C ubiquitin-specific protease DOA4 (DOA4), partial mRNA.

Price & Availability↑

CloneID	OSi01158
Clone ID Related Accession (Same CDS sequence)	NM_001180377.3
Accession Version	NM_001180377.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ubiquitin-specific protease DOA4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180377.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAGCAGA ATATTATTAG TACCATAAGG GATGAGTGTA TTCGTCACCG GTCGAAGTAC 
CTTACGATAG CACAACTAAC CGCTATTGCA GAGGCTAAAA TTAACGAATT CATCATAACT 
GGTAAGGCAA AAGATCAAGA TTTGAGCAGT CTTCTAGATA AATGCATCGA TATTTTATCT 
ATTTACAAGA AGAACTCGAA AGATATCAAA AATATTATAT CGTGCAAAAA TAAGGGTGCA 
ATGATTAGTT CAAATTCCGT AATGATTATT CAATTAAATT ATGTTTACTA CAAGGTAATT 
CACATTATTG TAACAACCAA TATTCCTCAT TTAAGTGAAT TCGCCAAGAT TAAATTACAT 
AAGAGCACGA GTGATGAGGG CAACGGTAAT AACAACAATA ATGAATTTCA ACTCATGAAC 
ATTTACAACA CTTTGCTGGA AACCTTATTA AAAGATGAAA ACATTGCAAA AATAAAAAGT 
TTCATTAAGT CTTCCATAAA ACAAACAAAA TTGAACCATG AGCAAGAAGA ATGTAACCTG 
ATGAGAACGG GTTCCTATAT CACTTCCAAT CAATTAAACT CCCTAATAAG TTCATCAGCA 
AATTCTGCTT CCTCCCAAAT GGAGATACTA CTGATAGATA TACGATCAAG GTTGGAATTC 
AACAAGTCAC ATATTGATAC AAAAAATATT ATATGCCTGG AGCCTATTTC TTTTAAAATG 
TCATATTCAG ATCATGATTT GGAGAAAAAA TCATTAATTA CTTCTCCTAA TAGTGAGATT 
AAAATGTTTC AAAGTAGAAA TCTTTTCAAG TTTATCATTC TCTATACAGA CGCAAACGAA 
TACAATGTTA AACAGCAGTC TGTCCTGTTG GACATTCTGG TGAATCATTC CTTTGAAAAA 
CCAATATCCG ATGACTTTAC CAAAATTTTC ATTCTGGAAT CTGGTTTTCC AGGTTGGCTT 
AAGTCAAATT ATGGGAGGCA AGTATCATCA TCTTTTCCAT CAAATAACAA TATTAAAGAT 
GATAGTGTTT ATATTAATGG TAACACTTCT GGCCTAAGTT TACAACATTT ACCTAAGATG 
TCTCCCAGTA TAAGACATTC AATGGACGAC TCTATGAAAG AAATGCTAGT TGCGCCTACT 
CCATTAAATC ATCTTCAACA ACAGCAACAA CAGCAATCAG ACAATGATCA TGTGCTAAAA 
AGATCTTCAA GTTTCAAAAA ATTATTCTCA AATTATACGT CTCCTAATCC GAAGAATTCA 
AATTCAAACT TATATTCTAT ATCTTCGTTG TCCATATCTA GTTCACCATC GCCTTTACCT 
CTACATTCGC CTGACCCAGT TAAGGGCAAT TCATTGCCAA TCAATTATCC GGAAACGCCA 
CATCTTTGGA AAAACAGTGA GACAGATTTT ATGACAAATC AAAGAGAACA GTTGAATCAC 
AACTCTTTTG CTCACATAGC TCCTATCAAC ACGAAGGCCA TCACTTCTCC ATCAAGAACT 
GCCACACCGA AGTTACAACG CTTCCCGCAA ACAATTAGTA TGAACCTTAA TATGAACTCC 
AATGGACACA GTTCTGCCAC CTCTACCATT CAACCTTCGT GTCTATCCTT GTCTAATAAT 
GACTCTTTAG ATCATACAGA TGTTACACCA ACTTCTTCTC ATAATTATGA CCTTGATTTC 
GCGGTTGGTT TGGAAAATCT AGGAAATTCG TGTTACATGA ACTGTATCAT TCAGTGTATC 
TTAGGTACAC ACGAATTAAC CCAAATCTTT TTGGACGATT CATATGCTAA ACACATCAAT 
ATTAATAGTA AGTTGGGATC GAAAGGTATT CTGGCAAAAT ATTTTGCAAG GTTGGTTCAT 
ATGATGTATA AGGAACAGGT TGATGGTTCA AAGAAAATTT CCATATCACC GATAAAATTT 
AAATTGGCAT GTGGATCTGT TAACTCATTA TTTAAGACTG CATCCCAACA GGACTGCCAA 
GAGTTTTGCC AATTCCTTCT AGATGGTCTT CATGAAGACT TGAACCAATG CGGTTCAAAC 
CCACCTTTGA AGGAGCTTTC TCAAGAAGCT GAGGCGAGAA GAGAAAAACT GTCTTTGCGA 
ATTGCCTCGT CAATTGAGTG GGAACGATTC TTGACTACTG ATTTCAGTGT TATTGTCGAC 
TTATTTCAGG GACAATACGC CTCACGACTA AAATGTAAAG TCTGTAGTCA TACCTCGACA 
ACATACCAAC CTTTTACGGT GCTGTCAATC CCTATTCCTA AAAAAAATTC CCGAAATAAT 
ATTACCATTG AAGATTGTTT CAGAGAGTTC ACCAAATGTG AGAACTTGGA AGTGGATGAG 
CAATGGTTGT GCCCACATTG TGAAAAAAGG CAGCCCTCCA CGAAACAATT GACAATAACG 
AGATTACCGA GGAATCTGAT AGTCCATTTA AAGAGATTTG ATAATTTATT AAACAAAAAT 
AATGACTTCG TCATATACCC TTTTTTGTTG GACTTGACTC CATTTTGGGC CAATGATTTT 
GACGGGGTTT TTCCTCCAGG TGTTAATGAC GATGAACTAC CAATAAGGGG ACAAATACCA 
CCTTTTAAGT ATGAATTATA TGGTGTAGCA TGCCACTTTG GTACTTTGTA TGGTGGTCAT 
TATACAGCCT ATGTGAAAAA GGGATTAAAG AAGGGATGGC TATATTTTGA TGATACCAAA 
TATAAACCTG TCAAAAACAA AGCCGATGCA ATTAACTCTA ATGCATACGT TTTGTTTTAT 
CACCGCGTCT ACGGTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010354.3
CDS	1..2781
Translation	MEQNIISTIRDECIRHRSKYLTIAQLTAIAEAKINEFIITGKAKDQDLSSLLDKCIDILSIYKKNSKDIKNIISCKNKGAMISSNSVMIIQLNYVYYKVIHIIVTTNIPHLSEFAKIKLHKSTSDEGNGNNNNNEFQLMNIYNTLLETLLKDENIAKIKSFIKSSIKQTKLNHEQEECNLMRTGSYITSNQLNSLISSSANSASSQMEILLIDIRSRLEFNKSHIDTKNIICLEPISFKMSYSDHDLEKKSLITSPNSEIKMFQSRNLFKFIILYTDANEYNVKQQSVLLDILVNHSFEKPISDDFTKIFILESGFPGWLKSNYGRQVSSSFPSNNNIKDDSVYINGNTSGLSLQHLPKMSPSIRHSMDDSMKEMLVAPTPLNHLQQQQQQQSDNDHVLKRSSSFKKLFSNYTSPNPKNSNSNLYSISSLSISSSPSPLPLHSPDPVKGNSLPINYPETPHLWKNSETDFMTNQREQLNHNSFAHIAPINTKAITSPSRTATPKLQRFPQTISMNLNMNSNGHSSATSTIQPSCLSLSNNDSLDHTDVTPTSSHNYDLDFAVGLENLGNSCYMNCIIQCILGTHELTQIFLDDSYAKHININSKLGSKGILAKYFARLVHMMYKEQVDGSKKISISPIKFKLACGSVNSLFKTASQQDCQEFCQFLLDGLHEDLNQCGSNPPLKELSQEAEARREKLSLRIASSIEWERFLTTDFSVIVDLFQGQYASRLKCKVCSHTSTTYQPFTVLSIPIPKKNSRNNITIEDCFREFTKCENLEVDEQWLCPHCEKRQPSTKQLTITRLPRNLIVHLKRFDNLLNKNNDFVIYPFLLDLTPFWANDFDGVFPPGVNDDELPIRGQIPPFKYELYGVACHFGTLYGGHYTAYVKKGLKKGWLYFDDTKYKPVKNKADAINSNAYVLFYHRVYGV

Target ORF information:

RefSeq Version	NM_001180377.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ubiquitin-specific protease DOA4 (DOA4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180377.3

ORF Insert Sequence:

ATGGAGCAGA ATATTATTAG TACCATAAGG GATGAGTGTA TTCGTCACCG GTCGAAGTAC 
CTTACGATAG CACAACTAAC CGCTATTGCA GAGGCTAAAA TTAACGAATT CATCATAACT 
GGTAAGGCAA AAGATCAAGA TTTGAGCAGT CTTCTAGATA AATGCATCGA TATTTTATCT 
ATTTACAAGA AGAACTCGAA AGATATCAAA AATATTATAT CGTGCAAAAA TAAGGGTGCA 
ATGATTAGTT CAAATTCCGT AATGATTATT CAATTAAATT ATGTTTACTA CAAGGTAATT 
CACATTATTG TAACAACCAA TATTCCTCAT TTAAGTGAAT TCGCCAAGAT TAAATTACAT 
AAGAGCACGA GTGATGAGGG CAACGGTAAT AACAACAATA ATGAATTTCA ACTCATGAAC 
ATTTACAACA CTTTGCTGGA AACCTTATTA AAAGATGAAA ACATTGCAAA AATAAAAAGT 
TTCATTAAGT CTTCCATAAA ACAAACAAAA TTGAACCATG AGCAAGAAGA ATGTAACCTG 
ATGAGAACGG GTTCCTATAT CACTTCCAAT CAATTAAACT CCCTAATAAG TTCATCAGCA 
AATTCTGCTT CCTCCCAAAT GGAGATACTA CTGATAGATA TACGATCAAG GTTGGAATTC 
AACAAGTCAC ATATTGATAC AAAAAATATT ATATGCCTGG AGCCTATTTC TTTTAAAATG 
TCATATTCAG ATCATGATTT GGAGAAAAAA TCATTAATTA CTTCTCCTAA TAGTGAGATT 
AAAATGTTTC AAAGTAGAAA TCTTTTCAAG TTTATCATTC TCTATACAGA CGCAAACGAA 
TACAATGTTA AACAGCAGTC TGTCCTGTTG GACATTCTGG TGAATCATTC CTTTGAAAAA 
CCAATATCCG ATGACTTTAC CAAAATTTTC ATTCTGGAAT CTGGTTTTCC AGGTTGGCTT 
AAGTCAAATT ATGGGAGGCA AGTATCATCA TCTTTTCCAT CAAATAACAA TATTAAAGAT 
GATAGTGTTT ATATTAATGG TAACACTTCT GGCCTAAGTT TACAACATTT ACCTAAGATG 
TCTCCCAGTA TAAGACATTC AATGGACGAC TCTATGAAAG AAATGCTAGT TGCGCCTACT 
CCATTAAATC ATCTTCAACA ACAGCAACAA CAGCAATCAG ACAATGATCA TGTGCTAAAA 
AGATCTTCAA GTTTCAAAAA ATTATTCTCA AATTATACGT CTCCTAATCC GAAGAATTCA 
AATTCAAACT TATATTCTAT ATCTTCGTTG TCCATATCTA GTTCACCATC GCCTTTACCT 
CTACATTCGC CTGACCCAGT TAAGGGCAAT TCATTGCCAA TCAATTATCC GGAAACGCCA 
CATCTTTGGA AAAACAGTGA GACAGATTTT ATGACAAATC AAAGAGAACA GTTGAATCAC 
AACTCTTTTG CTCACATAGC TCCTATCAAC ACGAAGGCCA TCACTTCTCC ATCAAGAACT 
GCCACACCGA AGTTACAACG CTTCCCGCAA ACAATTAGTA TGAACCTTAA TATGAACTCC 
AATGGACACA GTTCTGCCAC CTCTACCATT CAACCTTCGT GTCTATCCTT GTCTAATAAT 
GACTCTTTAG ATCATACAGA TGTTACACCA ACTTCTTCTC ATAATTATGA CCTTGATTTC 
GCGGTTGGTT TGGAAAATCT AGGAAATTCG TGTTACATGA ACTGTATCAT TCAGTGTATC 
TTAGGTACAC ACGAATTAAC CCAAATCTTT TTGGACGATT CATATGCTAA ACACATCAAT 
ATTAATAGTA AGTTGGGATC GAAAGGTATT CTGGCAAAAT ATTTTGCAAG GTTGGTTCAT 
ATGATGTATA AGGAACAGGT TGATGGTTCA AAGAAAATTT CCATATCACC GATAAAATTT 
AAATTGGCAT GTGGATCTGT TAACTCATTA TTTAAGACTG CATCCCAACA GGACTGCCAA 
GAGTTTTGCC AATTCCTTCT AGATGGTCTT CATGAAGACT TGAACCAATG CGGTTCAAAC 
CCACCTTTGA AGGAGCTTTC TCAAGAAGCT GAGGCGAGAA GAGAAAAACT GTCTTTGCGA 
ATTGCCTCGT CAATTGAGTG GGAACGATTC TTGACTACTG ATTTCAGTGT TATTGTCGAC 
TTATTTCAGG GACAATACGC CTCACGACTA AAATGTAAAG TCTGTAGTCA TACCTCGACA 
ACATACCAAC CTTTTACGGT GCTGTCAATC CCTATTCCTA AAAAAAATTC CCGAAATAAT 
ATTACCATTG AAGATTGTTT CAGAGAGTTC ACCAAATGTG AGAACTTGGA AGTGGATGAG 
CAATGGTTGT GCCCACATTG TGAAAAAAGG CAGCCCTCCA CGAAACAATT GACAATAACG 
AGATTACCGA GGAATCTGAT AGTCCATTTA AAGAGATTTG ATAATTTATT AAACAAAAAT 
AATGACTTCG TCATATACCC TTTTTTGTTG GACTTGACTC CATTTTGGGC CAATGATTTT 
GACGGGGTTT TTCCTCCAGG TGTTAATGAC GATGAACTAC CAATAAGGGG ACAAATACCA 
CCTTTTAAGT ATGAATTATA TGGTGTAGCA TGCCACTTTG GTACTTTGTA TGGTGGTCAT 
TATACAGCCT ATGTGAAAAA GGGATTAAAG AAGGGATGGC TATATTTTGA TGATACCAAA 
TATAAACCTG TCAAAAACAA AGCCGATGCA ATTAACTCTA ATGCATACGT TTTGTTTTAT 
CACCGCGTCT ACGGTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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DOA4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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