CDC34 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC34
Entrez Gene ID	851624
Full Name	SCF E2 ubiquitin-protein ligase catalytic subunit CDC34
Synonyms	DNA6,UBC3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180362.1	NP_010339.1	SCF E2 ubiquitin-protein ligase catalytic subunit CDC34

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UBE2R2	NP_001193039.1
Neurospora crassa	NCU01188	XP_961554.1
Xenopus tropicalis (tropical clawed frog)	ube2r2	NP_001072702.1
Canis lupus familiaris (dog)	UBE2R2	XP_854606.3
Caenorhabditis elegans (roundworm)	ubc-3	NP_490882.3
Saccharomyces cerevisiae (baker's yeast)	CDC34	NP_010339.1
Magnaporthe oryzae (rice blast fungus)	MGG_14266	XP_003711064.1
Mus musculus (house mouse)	Ube2r2	NP_080551.1
Gallus gallus (chicken)	LOC427134	XP_424727.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008680	XP_314780.3
Pan troglodytes (chimpanzee)	UBE2R2	XP_001155644.3
Danio rerio (zebrafish)	ube2r2	NP_001002600.1
Kluyveromyces lactis	KLLA0D09196g	XP_453472.1
Eremothecium gossypii	AGOS_AGL203C	NP_986464.1
Homo sapiens (human)	UBE2R2	NP_060281.2
	LOC701507	XP_001089803.2
Rattus norvegicus (Norway rat)	LOC689226	NP_001121045.1
Drosophila melanogaster (fruit fly)	CG7656	NP_001261885.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdc34 showed a strong preference for Ub lysine 48 (K48), with lower activity towards lysine 11 (K11) and lysine 63 (K63).
Title: Molecular and structural insight into lysine selection on substrate and ubiquitin lysine 48 by the ubiquitin-conjugating enzyme Cdc34.

Cdc34 is particularly sensitive to oxidative inactivation, through sequestration of the catalytic cysteine in a disulfide complex with Uba1, by levels of oxidant that do not reduce global ubiquitinylation of proteins.
Title: Oxidative stress responses involve oxidation of a conserved ubiquitin pathway enzyme.

chromosome segregation in cdc6 mutant is caused by the deregulation of spindle dynamics induced via a regulatory network involving the ubiquitin-conjugating enzyme Cdc34, microtubule-associated proteins and the anaphase-promoting complex activator Cdh1.
Title: "Reductional anaphase" in replication-defective cells is caused by ubiquitin-conjugating enzyme Cdc34-mediated deregulation of the spindle.

Through analysis of the phenotype of a mutant missing a highly conserved sequence motif within the catalytic domain of Cdc34, we discovered previously unrecognized levels of regulation
Title: New insight into the role of the Cdc34 ubiquitin-conjugating enzyme in cell cycle regulation via Ace2 and Sic1.

Cdc34 phosphorylation by PKA and Sch9 provides a direct tether between G1 cell division events and cell growth
Title: Nutrient sensing kinases PKA and Sch9 phosphorylate the catalytic domain of the ubiquitin-conjugating enzyme Cdc34.

The following CDC34 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC34 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01143	NM_001180362.1 Latest version!	Saccharomyces cerevisiae S288C SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 (CDC34), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC34 ( NM_001180362.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010339.1 Saccharomyces cerevisiae S288C SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 (CDC34), partial mRNA.

Price & Availability↑

CloneID	OSi01143
Clone ID Related Accession (Same CDS sequence)	NM_001180362.1
Accession Version	NM_001180362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SCF E2 ubiquitin-protein ligase catalytic subunit CDC34
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTAGTC GCAAAAGCAC CGCTTCTAGC TTACTGTTAC GGCAATATAG AGAACTTACT 
GATCCTAAGA AAGCTATTCC CTCATTTCAT ATAGAACTAG AGGATGATTC AAATATTTTC 
ACTTGGAACA TAGGTGTTAT GGTGCTAAAT GAGGATTCCA TTTATCATGG AGGGTTTTTC 
AAAGCTCAAA TGAGATTTCC GGAAGACTTT CCCTTTTCTC CACCACAGTT TCGATTTACG 
CCGGCTATCT ACCATCCAAA CGTTTACAGG GATGGCAGGC TTTGTATTTC TATTTTACAT 
CAAAGTGGGG ATCCTATGAC CGACGAACCT GATGCTGAAA CGTGGTCCCC CGTGCAGACC 
GTGGAAAGTG TGTTGATCTC TATAGTATCT CTATTAGAGG ACCCCAATAT CAACTCGCCA 
GCAAATGTCG ATGCCGCTGT TGATTACAGA AAGAACCCTG AACAATACAA GCAAAGAGTC 
AAAATGGAAG TGGAAAGATC GAAACAAGAT ATCCCTAAAG GTTTCATAAT GCCCACTTCT 
GAATCGGCGT ACATATCTCA AAGTAAACTA GATGAACCAG AATCGAATAA GGATATGGCG 
GATAATTTTT GGTATGATAG TGATTTGGAC GACGATGAGA ACGGGTCCGT CATTTTACAA 
GACGACGACT ACGACGATGG AAACAACCAT ATCCCCTTTG AAGATGATGA CGTTTACAAC 
TATAACGACA ACGACGACGA TGACGAAAGA ATCGAATTCG AAGATGATGA CGATGATGAC 
GACGATAGTA TAGATAACGA CAGCGTCATG GATAGGAAAC AACCCCACAA GGCTGAAGAT 
GAAAGTGAAG ATGTGGAGGA TGTAGAAAGA GTTTCAAAGA AAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010339.1
CDS	1..888
Translation	MSSRKSTASSLLLRQYRELTDPKKAIPSFHIELEDDSNIFTWNIGVMVLNEDSIYHGGFFKAQMRFPEDFPFSPPQFRFTPAIYHPNVYRDGRLCISILHQSGDPMTDEPDAETWSPVQTVESVLISIVSLLEDPNINSPANVDAAVDYRKNPEQYKQRVKMEVERSKQDIPKGFIMPTSESAYISQSKLDEPESNKDMADNFWYDSDLDDDENGSVILQDDDYDDGNNHIPFEDDDVYNYNDNDDDDERIEFEDDDDDDDDSIDNDSVMDRKQPHKAEDESEDVEDVERVSKKI

Target ORF information:

RefSeq Version	NM_001180362.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 (CDC34), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180362.1

ORF Insert Sequence:

ATGAGTAGTC GCAAAAGCAC CGCTTCTAGC TTACTGTTAC GGCAATATAG AGAACTTACT 
GATCCTAAGA AAGCTATTCC CTCATTTCAT ATAGAACTAG AGGATGATTC AAATATTTTC 
ACTTGGAACA TAGGTGTTAT GGTGCTAAAT GAGGATTCCA TTTATCATGG AGGGTTTTTC 
AAAGCTCAAA TGAGATTTCC GGAAGACTTT CCCTTTTCTC CACCACAGTT TCGATTTACG 
CCGGCTATCT ACCATCCAAA CGTTTACAGG GATGGCAGGC TTTGTATTTC TATTTTACAT 
CAAAGTGGGG ATCCTATGAC CGACGAACCT GATGCTGAAA CGTGGTCCCC CGTGCAGACC 
GTGGAAAGTG TGTTGATCTC TATAGTATCT CTATTAGAGG ACCCCAATAT CAACTCGCCA 
GCAAATGTCG ATGCCGCTGT TGATTACAGA AAGAACCCTG AACAATACAA GCAAAGAGTC 
AAAATGGAAG TGGAAAGATC GAAACAAGAT ATCCCTAAAG GTTTCATAAT GCCCACTTCT 
GAATCGGCGT ACATATCTCA AAGTAAACTA GATGAACCAG AATCGAATAA GGATATGGCG 
GATAATTTTT GGTATGATAG TGATTTGGAC GACGATGAGA ACGGGTCCGT CATTTTACAA 
GACGACGACT ACGACGATGG AAACAACCAT ATCCCCTTTG AAGATGATGA CGTTTACAAC 
TATAACGACA ACGACGACGA TGACGAAAGA ATCGAATTCG AAGATGATGA CGATGATGAC 
GACGATAGTA TAGATAACGA CAGCGTCATG GATAGGAAAC AACCCCACAA GGCTGAAGAT 
GAAAGTGAAG ATGTGGAGGA TGTAGAAAGA GTTTCAAAGA AAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CDC34 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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