REG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	REG1
Entrez Gene ID	851592
Full Name	protein phosphatase regulator REG1
Synonyms	HEX2,PZF240,SPP43,SRN1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180336.1	NP_010311.1	protein phosphatase regulator REG1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F04345g	XP_455276.1
Saccharomyces cerevisiae (baker's yeast)	REG1	NP_010311.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we show that the defect in glucose-repression in the absence of Ssb is due to the ability of the chaperone to bridge between the SNF1 and Glc7 complexes. Ssb performs this post-translational function in concert with the 14-3-3 protein Bmh, to which Ssb binds via its very C-terminus. Raising the intracellular concentration of Ssb or Bmh enabled Glc7 to dephosphorylate SNF1 even in the absence of Reg1
Title: The Hsp70 homolog Ssb and the 14-3-3 protein Bmh1 jointly regulate transcription of glucose repressed genes in Saccharomyces cerevisiae.

Data show that the Reg1-Glc7 protein phosphatase 1 and Sit4 type 2A-like phosphatase are largely responsible for dephosphorylation of Thr-210 of Snf1(1-309).
Title: Heterotrimer-independent regulation of activation-loop phosphorylation of Snf1 protein kinase involves two protein phosphatases.

the ability of Reg1 to promote the dephosphorylation of Snf1 is not directly related to the strength of its association with the Snf1 complex
Title: Reg1 protein regulates phosphorylation of all three Snf1 isoforms but preferentially associates with the Gal83 isoform.

Roles of two protein phosphatases, Reg1-Glc7 and Sit4, and glycogen synthesis in regulation of SNF1 protein kinase.
Title: Roles of two protein phosphatases, Reg1-Glc7 and Sit4, and glycogen synthesis in regulation of SNF1 protein kinase.

data suggest that the regulation of Snf1 dephosphorylation is imparted through a dynamic competition between the Glc7 phosphatase and the Snf1 kinase for binding to the PP1 regulatory subunit Reg1.
Title: PP1 phosphatase-binding motif in Reg1 protein of Saccharomyces cerevisiae is required for interaction with both the PP1 phosphatase Glc7 and the Snf1 protein kinase.

The following REG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the REG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01116	NM_001180336.1 Latest version!	Saccharomyces cerevisiae S288C protein phosphatase regulator REG1 (REG1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

REG1 ( NM_001180336.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010311.1 Saccharomyces cerevisiae S288C protein phosphatase regulator REG1 (REG1), partial mRNA.

Price & Availability↑

CloneID	OSi01116
Clone ID Related Accession (Same CDS sequence)	NM_001180336.1
Accession Version	NM_001180336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein phosphatase regulator REG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAACAA ATCTAGCAAA TTACTTCGCC GGTAAGAAAG ATATTGAAAA CGAGCATGTA 
AATAGAAATG CGAGCCATGA AAGTAATAGC AAAAGTGATG TCAAAATTAG TGGTAACGAT 
AACGACAACG ACGAAGATAT GGGGCCTTCA GTATCGATGG CTGTTCAAGC GAAAAATGAT 
GATGATTTCC ATAAATCAAC TTTCAACCTT AAAAGAACAA GATCAATGGG CCTTCTTGAT 
GAATATATTG ACCCTACCAA GAAATTGCTA GGAAGGTCAG ACGACTTATA TGATAACGAC 
AATGAATATT ATGACAACTC ATCTAATAAT TCTTCAAGCA ATTCTTCAGA TGATGATTAT 
GATGACGGCT ATCAGGAACA CTCAACCTCC GTTTCTCCAC CGCCGGCGGA TAATGATAGC 
TACTTAATTC CACAAGATGA TAATGACGTA GTGGTAGAGC CAGAAAGACA TGTTGACTAT 
CTGTCACATG AATGGAAAGA ATCAGAAATT TCTAATTCTT GGAAATATAT CATCCTAAAG 
AAAAAGAAAA GAGATGTTGA TCTAGTAAAT GCTGCTAGGT TAGAAAATGC CTCATGGAGA 
ACATGGGCAA AAGCAAGAAA CAACTTGAAA ACAGTGTCAC CTGAAGTGGT TAATTGGTCA 
AAAGATTCTG ATGTGACTTG GCTATATGGC CCTATTGTAA GAGATTCTGA AGGTAATGCC 
CAAAGTGAAG AAGAACATGA TCTAGAAAGA GGATATGGTT CGGATGATGA AAATTCCAAG 
AGAATTTCAA TGCCCACTAA AAATTCAAAG TCGATCGCAG CTGCTCCGAA ACCAATTTTG 
AAGAAGAGAA CCGTCACAGA AATTATAGAG GACAACGCTT TATGGAAACT GAATGAGGCA 
AGAAAACACA TGACAGAAAT GAAACATGCT TCGGTAATAA TGGATCCCAA TGGCAACAAA 
AACGTCCATG ACGATTTCGA TGCTCTGGCC GCTCAAGTTA ACGCCCAGTA CTACCATTAT 
CCCAAAGAAT CGAACAGTAG CGTTAGTTTG AAGAGTCAAC ACTCTGACAA AAAAGATAAT 
TCTACCATAC CGAATCCTGT AGGAGAAAAT TCAAATGGTG GCGGTGACAA GGGAGAAGAA 
GACCTGCATT TAAAGTCAGC GCTTCATGTT CAAAACAATA GGTCAACGGC ACAATCAAAC 
AAAAGCATAC TAGAAAATAG TACCAATGAT CGTAAGGCTA ACTTGGATCA AAACCTAAAT 
TCTCCTGATA ATAATAGGTT CCCCTCCTCA ACATCTTCCT CCAATAGAGA TAATGAAAAC 
AATTCCATGG GATTATCATC AATATTAACC TCAAACCCAA GTGAAAAATC TAACAAACCT 
ACTAAAAATA GACATATACA TTTTAATGAC AGGGTGGAAC AATGTATGGC ACTACGATAT 
CCAGCTTCAC AATCGGAGGA TGATGAAAGC GATGATGAAA ATAAACAATA TGTTGATGTT 
AATAACAATG CGAACGTTAC AACAATAAAC AACAATAGGA CTCCATTGCT AGCCATTCAG 
CATAAATCTA TTCCTATCAA CTCCGCAACT GAACATTTGA ACAAAAATAC TAGCGACGAT 
GATACATCCT CACAATCATC ATCTTCATCA CATTCTGATG ATGAAGAACA CGGCGGCCTT 
TACATAAATG CAAGATTTTC CAGGAGGTCT GACTCTGGAG TTCATTCACC AATAACAGAT 
AATTCCTCTG TGGCTTCATC TACTACTTCC AGAGCTCATG TACGTCCCAT AATAAAGTTG 
CTTCCTGATA CCACTTTGAA TTATGGATCA GACGAAGAAT CTGATAATGG CGAATTTAAT 
GGGTATGGTA ATGCTGTGTC ACATAACGTC AATACCTCCA GAGGCTATGA TTACATATAC 
GATTATAACT CGGTCTACAC TGGTGATACT TCCAGTTTTT TACCAGTAGA TTCATGTGAC 
ATTGTAGATG TTCCTGAAGG AATGGACTTA CAAACAGCCA TTGCAGATGA TAATGCATCA 
AACTATGAAT TTAACAATGC GGTAGAATCC AAGGAAAAAC ATGTTCCACA ACTACACAAA 
GCTTCAGCAA ATAATACAAC ACGTCAACAT GGATCGCATA TGTTATTATA TGACGACGAC 
AATTACAGCT CTTCATCAGA TAGCGAACAG CAATTTATTG AAGACTCACA ATACAATAGT 
AGCGACGACG AAGAGGAAGA AGATGACGAT GACCAAGAAG TAGACGATAA CCACGATGAA 
GGACTTTCAC TGAGGAGAAC ATTGTCACTT GGGAAATCAG GATCTACCAA TTCTTTGTAC 
GATCTAGCAC AACCATCACT CTCTTCGGCA ACTCCTCAAC AAAAAAATCC TACTAATTTT 
ACAGGGGGTA AAACTGATGT AGACAAAGAT GCACAACTGG CTGTCAGGCC GTATCCGTTG 
AAGCGCAATT CCTCTTCAGG GAACTTCATA TTCAATTCAG ATAGTGAAGA AGAGAGTAGC 
AGCGAGGAGG AACAAAGACC ATTACCCGCT AACAGTCAGC TGGTTAATCG AAGCGTATTG 
AAAGGCAGTG TGACACCAGC AAACATATCA TCTCAGAAGA AAAAAGCCCT TCCAAAGCAG 
CCAAAAGCGT CCGATAGTTC ACAAAGCTTT AGAATTGTAA ATAATACTCC TTCACCAGCC 
GAAGTGGGGG CGAGTGATGT TGCCATAGAG GGTTATTTCT CTCCTAGAAA TGAATCTATC 
AAGTCTGTTG TTTCCGGGGG AAATATGATG GACCATCAAG ATCACTCAGA AATGGACACT 
TTGGCAAAAG GATTTGAAAA CTGCCATATA AATAATGCTA GTAAGCTAAA GGACAAGAAA 
GTTGATAGCG TGCAAACCAC TAGAAAAGAA GCGTCTTTGA CGGATTCGAG TAATGAAAGC 
TTACACAAAG TGGTGCAGAA TGCAAGAGGT ATGGCAAGCA AGTACTTGCA CTCTTGGAAA 
AAGAGTGACG TCAAGCCACA AGAAAATGGA AATGACAGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010311.1
CDS	1..3045
Translation	MSTNLANYFAGKKDIENEHVNRNASHESNSKSDVKISGNDNDNDEDMGPSVSMAVQAKNDDDFHKSTFNLKRTRSMGLLDEYIDPTKKLLGRSDDLYDNDNEYYDNSSNNSSSNSSDDDYDDGYQEHSTSVSPPPADNDSYLIPQDDNDVVVEPERHVDYLSHEWKESEISNSWKYIILKKKKRDVDLVNAARLENASWRTWAKARNNLKTVSPEVVNWSKDSDVTWLYGPIVRDSEGNAQSEEEHDLERGYGSDDENSKRISMPTKNSKSIAAAPKPILKKRTVTEIIEDNALWKLNEARKHMTEMKHASVIMDPNGNKNVHDDFDALAAQVNAQYYHYPKESNSSVSLKSQHSDKKDNSTIPNPVGENSNGGGDKGEEDLHLKSALHVQNNRSTAQSNKSILENSTNDRKANLDQNLNSPDNNRFPSSTSSSNRDNENNSMGLSSILTSNPSEKSNKPTKNRHIHFNDRVEQCMALRYPASQSEDDESDDENKQYVDVNNNANVTTINNNRTPLLAIQHKSIPINSATEHLNKNTSDDDTSSQSSSSSHSDDEEHGGLYINARFSRRSDSGVHSPITDNSSVASSTTSRAHVRPIIKLLPDTTLNYGSDEESDNGEFNGYGNAVSHNVNTSRGYDYIYDYNSVYTGDTSSFLPVDSCDIVDVPEGMDLQTAIADDNASNYEFNNAVESKEKHVPQLHKASANNTTRQHGSHMLLYDDDNYSSSSDSEQQFIEDSQYNSSDDEEEEDDDDQEVDDNHDEGLSLRRTLSLGKSGSTNSLYDLAQPSLSSATPQQKNPTNFTGGKTDVDKDAQLAVRPYPLKRNSSSGNFIFNSDSEEESSSEEEQRPLPANSQLVNRSVLKGSVTPANISSQKKKALPKQPKASDSSQSFRIVNNTPSPAEVGASDVAIEGYFSPRNESIKSVVSGGNMMDHQDHSEMDTLAKGFENCHINNASKLKDKKVDSVQTTRKEASLTDSSNESLHKVVQNARGMASKYLHSWKKSDVKPQENGNDSS

Target ORF information:

RefSeq Version	NM_001180336.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein phosphatase regulator REG1 (REG1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180336.1

ORF Insert Sequence:

ATGTCAACAA ATCTAGCAAA TTACTTCGCC GGTAAGAAAG ATATTGAAAA CGAGCATGTA 
AATAGAAATG CGAGCCATGA AAGTAATAGC AAAAGTGATG TCAAAATTAG TGGTAACGAT 
AACGACAACG ACGAAGATAT GGGGCCTTCA GTATCGATGG CTGTTCAAGC GAAAAATGAT 
GATGATTTCC ATAAATCAAC TTTCAACCTT AAAAGAACAA GATCAATGGG CCTTCTTGAT 
GAATATATTG ACCCTACCAA GAAATTGCTA GGAAGGTCAG ACGACTTATA TGATAACGAC 
AATGAATATT ATGACAACTC ATCTAATAAT TCTTCAAGCA ATTCTTCAGA TGATGATTAT 
GATGACGGCT ATCAGGAACA CTCAACCTCC GTTTCTCCAC CGCCGGCGGA TAATGATAGC 
TACTTAATTC CACAAGATGA TAATGACGTA GTGGTAGAGC CAGAAAGACA TGTTGACTAT 
CTGTCACATG AATGGAAAGA ATCAGAAATT TCTAATTCTT GGAAATATAT CATCCTAAAG 
AAAAAGAAAA GAGATGTTGA TCTAGTAAAT GCTGCTAGGT TAGAAAATGC CTCATGGAGA 
ACATGGGCAA AAGCAAGAAA CAACTTGAAA ACAGTGTCAC CTGAAGTGGT TAATTGGTCA 
AAAGATTCTG ATGTGACTTG GCTATATGGC CCTATTGTAA GAGATTCTGA AGGTAATGCC 
CAAAGTGAAG AAGAACATGA TCTAGAAAGA GGATATGGTT CGGATGATGA AAATTCCAAG 
AGAATTTCAA TGCCCACTAA AAATTCAAAG TCGATCGCAG CTGCTCCGAA ACCAATTTTG 
AAGAAGAGAA CCGTCACAGA AATTATAGAG GACAACGCTT TATGGAAACT GAATGAGGCA 
AGAAAACACA TGACAGAAAT GAAACATGCT TCGGTAATAA TGGATCCCAA TGGCAACAAA 
AACGTCCATG ACGATTTCGA TGCTCTGGCC GCTCAAGTTA ACGCCCAGTA CTACCATTAT 
CCCAAAGAAT CGAACAGTAG CGTTAGTTTG AAGAGTCAAC ACTCTGACAA AAAAGATAAT 
TCTACCATAC CGAATCCTGT AGGAGAAAAT TCAAATGGTG GCGGTGACAA GGGAGAAGAA 
GACCTGCATT TAAAGTCAGC GCTTCATGTT CAAAACAATA GGTCAACGGC ACAATCAAAC 
AAAAGCATAC TAGAAAATAG TACCAATGAT CGTAAGGCTA ACTTGGATCA AAACCTAAAT 
TCTCCTGATA ATAATAGGTT CCCCTCCTCA ACATCTTCCT CCAATAGAGA TAATGAAAAC 
AATTCCATGG GATTATCATC AATATTAACC TCAAACCCAA GTGAAAAATC TAACAAACCT 
ACTAAAAATA GACATATACA TTTTAATGAC AGGGTGGAAC AATGTATGGC ACTACGATAT 
CCAGCTTCAC AATCGGAGGA TGATGAAAGC GATGATGAAA ATAAACAATA TGTTGATGTT 
AATAACAATG CGAACGTTAC AACAATAAAC AACAATAGGA CTCCATTGCT AGCCATTCAG 
CATAAATCTA TTCCTATCAA CTCCGCAACT GAACATTTGA ACAAAAATAC TAGCGACGAT 
GATACATCCT CACAATCATC ATCTTCATCA CATTCTGATG ATGAAGAACA CGGCGGCCTT 
TACATAAATG CAAGATTTTC CAGGAGGTCT GACTCTGGAG TTCATTCACC AATAACAGAT 
AATTCCTCTG TGGCTTCATC TACTACTTCC AGAGCTCATG TACGTCCCAT AATAAAGTTG 
CTTCCTGATA CCACTTTGAA TTATGGATCA GACGAAGAAT CTGATAATGG CGAATTTAAT 
GGGTATGGTA ATGCTGTGTC ACATAACGTC AATACCTCCA GAGGCTATGA TTACATATAC 
GATTATAACT CGGTCTACAC TGGTGATACT TCCAGTTTTT TACCAGTAGA TTCATGTGAC 
ATTGTAGATG TTCCTGAAGG AATGGACTTA CAAACAGCCA TTGCAGATGA TAATGCATCA 
AACTATGAAT TTAACAATGC GGTAGAATCC AAGGAAAAAC ATGTTCCACA ACTACACAAA 
GCTTCAGCAA ATAATACAAC ACGTCAACAT GGATCGCATA TGTTATTATA TGACGACGAC 
AATTACAGCT CTTCATCAGA TAGCGAACAG CAATTTATTG AAGACTCACA ATACAATAGT 
AGCGACGACG AAGAGGAAGA AGATGACGAT GACCAAGAAG TAGACGATAA CCACGATGAA 
GGACTTTCAC TGAGGAGAAC ATTGTCACTT GGGAAATCAG GATCTACCAA TTCTTTGTAC 
GATCTAGCAC AACCATCACT CTCTTCGGCA ACTCCTCAAC AAAAAAATCC TACTAATTTT 
ACAGGGGGTA AAACTGATGT AGACAAAGAT GCACAACTGG CTGTCAGGCC GTATCCGTTG 
AAGCGCAATT CCTCTTCAGG GAACTTCATA TTCAATTCAG ATAGTGAAGA AGAGAGTAGC 
AGCGAGGAGG AACAAAGACC ATTACCCGCT AACAGTCAGC TGGTTAATCG AAGCGTATTG 
AAAGGCAGTG TGACACCAGC AAACATATCA TCTCAGAAGA AAAAAGCCCT TCCAAAGCAG 
CCAAAAGCGT CCGATAGTTC ACAAAGCTTT AGAATTGTAA ATAATACTCC TTCACCAGCC 
GAAGTGGGGG CGAGTGATGT TGCCATAGAG GGTTATTTCT CTCCTAGAAA TGAATCTATC 
AAGTCTGTTG TTTCCGGGGG AAATATGATG GACCATCAAG ATCACTCAGA AATGGACACT 
TTGGCAAAAG GATTTGAAAA CTGCCATATA AATAATGCTA GTAAGCTAAA GGACAAGAAA 
GTTGATAGCG TGCAAACCAC TAGAAAAGAA GCGTCTTTGA CGGATTCGAG TAATGAAAGC 
TTACACAAAG TGGTGCAGAA TGCAAGAGGT ATGGCAAGCA AGTACTTGCA CTCTTGGAAA 
AAGAGTGACG TCAAGCCACA AGAAAATGGA AATGACAGCA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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REG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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